LOCUS       sequence-417395-       15861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac plasmid with dCas9 docking array with four tag domains 
            (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15861)
  AUTHORS   Swain T, Pflueger C, Freytag S, Poppe D, Pflueger J, Nguyen TV, Li 
            JK, Lister R
  TITLE     A modular dCas9-based recruitment platform for combinatorial 
            epigenome editing.
  JOURNAL   Nucleic Acids Res. 2023 Nov 24:gkad1108. doi: 10.1093/nar/gkad1108.
  PUBMED    38000387
REFERENCE   2  (bases 1 to 15861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Nov 24:gkad1108. doi: 10.1093/nar/gkad1108."
COMMENT     Sequence Label: LLP791_PB_dCas9-SSSavi_BirA
FEATURES             Location/Qualifiers
     source          1..15861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   2340..2374
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        2753..3132
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3412..4428
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4352..4374
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4436..4455
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    4522..4546
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4552..4572
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4573..4599
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4603..4629
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4633..4659
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(4693..4719,4720..4722,4723..7206,7207..7212,7213..7278,
                     7279..7281,7282..8796)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(4693..4719,4720..4722,4723..7209,7210..7212,
                     7213..8796)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4693..8796
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8833..8853
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8893..8913
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8935..8973
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     CDS             9043..9078
                     /label=SnoopTag
     CDS             9145..9201
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9271..9327
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9394..9450
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9517..9573
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9640..9696
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9757..9801
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             9826..9846
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9866..9885)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9910..9957
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    10124..10247
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        10301..11479
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          10532..11470
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     11427..11447
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        11496..11514
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             11566..12531
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             11566..12528
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     protein_bind    complement(12533..12557)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    12599..13171
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    12621..13173
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(12765..12782)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     12992..13011
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             13173..14198
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDEIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(14287..14306)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    14352..14407
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(14406..14425)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     repeat_region   15094..15156
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(15223..15241)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(15248..15265)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(15248..15264)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15257..15279)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      15406..15834
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15493..15512)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15703..15724
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggtacctc gcgcgacttg
     2221 gtttgccatt ctttagcgcg cgtcgcgtca cacagcttgg ccacaatgtg gtttttgtca
     2281 aacgaagatt ctatgacgtg tttaaagttt aggtcgagta aagcgcaaat cttttttaac
     2341 cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg ctctctcttt
     2401 ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc ttggagctcc
     2461 cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa cgaccgcgtg
     2521 agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc atacgataat
     2581 tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat tttcttgtta
     2641 tagatatcgt gactaatata taataaaatg ggtagttctt tagacgatga gcatatcctc
     2701 tctgctcttc tgcaaagcga tgacgagctt gttggctagc ggtggcggcc tcgacattga
     2761 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     2821 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     2881 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     2941 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     3001 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     3061 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     3121 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
     3181 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
     3241 gggggggggg gggggcgcgc gccggggggg ggggggsggg gsggggggsg gggsggggsg
     3301 rggsggrgag gkgsggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg
     3361 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct
     3421 gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc
     3481 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt
     3541 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag
     3601 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg
     3661 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc
     3721 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc
     3781 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg
     3841 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc
     3901 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg
     3961 ctcgccgtgc cgggcggggg gtggcggcag gkgggggtgc cgggcggggc ggggccgccy
     4021 cgggccgggg ggggckcggg ggrggggcgc ggcggccccc ggagcgccgg cggctgtcga
     4081 ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt
     4141 cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc
     4201 gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt
     4261 gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac
     4321 ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc
     4381 tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac
     4441 gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcggccgc ggtaccggcg
     4501 cgccggatcc tgcaggttat cacaagtttg tacaaaaaag caggcttaat gccaaagaag
     4561 aagcggaagg tctaccccta tgacgtgcct gattacgccg cttatcctta cgatgtgccc
     4621 gactatgcag cctacccata cgatgtgccc gactacgccg caatggccgg tatccacgga
     4681 gtcccagcag ccatggacaa gaagtactcc attgggctcg ctatcggcac aaacagcgtc
     4741 ggctgggccg tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc
     4801 aataccgatc gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg
     4861 gagacggccg aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag
     4921 aatcggatct gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct
     4981 ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac
     5041 ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat
     5101 catctgagga agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc
     5161 gcgctggcgc atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca
     5221 gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa tcagcttttc
     5281 gaagagaacc cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg
     5341 tccaaatccc ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc
     5401 ctgtttggta atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc
     5461 gacctggccg aagatgccaa gcttcaactg agcaaagaca cctacgatga tgatctcgac
     5521 aatctgctgg cccagatcgg cgaccagtac gcagaccttt ttttggcggc aaagaacctg
     5581 tcagacgcca ttctgctgag tgatattctg cgagtgaaca cggagatcac caaagctccg
     5641 ctgagcgcta gtatgatcaa gcgctatgat gagcaccacc aagacttgac tttgctgaag
     5701 gcccttgtca gacagcaact gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa
     5761 aatggctacg ccggatacat tgacggcgga gcaagccagg aggaatttta caaatttatt
     5821 aagcccatct tggaaaaaat ggacggcacc gaggagctgc tggtaaagct taacagagaa
     5881 gatctgttgc gcaaacagcg cactttcgac aatggaagca tcccccacca gattcacctg
     5941 ggcgaactgc acgctatcct caggcggcaa gaggatttct accccttttt gaaagataac
     6001 agggaaaaga ttgagaaaat cctcacattt cggataccct actatgtggg ccccctcgcc
     6061 cggggaaatt ccagattcgc gtggatgact cgcaaatcag aagagaccat cactccctgg
     6121 aacttcgagg aagtcgtgga taagggggcc tctgcccagt ccttcatcga aaggatgact
     6181 aactttgata aaaatctgcc taacgaaaag gtgcttccta aacactctct gctgtacgag
     6241 tacttcacag tttataacga gctcaccaag gtcaaatacg tcacagaagg gatgagaaag
     6301 ccagcattcc tgtctggaga gcagaagaaa gctatcgtgg acctcctctt caagacgaac
     6361 cggaaagtta ccgtgaaaca gctcaaagaa gactatttca aaaagattga atgtttcgac
     6421 tctgttgaaa tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat
     6481 ctcctgaaaa tcattaaaga caaggacttc ctggacaatg aggagaacga ggacattctt
     6541 gaggacattg tcctcaccct tacgttgttt gaagataggg agatgattga agaacgcttg
     6601 aaaacttacg ctcatctctt cgacgacaaa gtcatgaaac agctcaagag gcgccgatat
     6661 acaggatggg ggcggctgtc aagaaaactg atcaatggga tccgagacaa gcagagtgga
     6721 aagacaatcc tggattttct taagtccgat ggatttgcca acaggaactt catgcagttg
     6781 atccatgatg actctctcac ctttaaggag gacatccaga aagcacaagt ttctggccag
     6841 ggggacagtc ttcacgagca catcgctaat cttgcaggta gcccagctat caaaaaggga
     6901 atactgcaga ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag gcataagccc
     6961 gagaatatcg ttatcgagat ggcccgagag aaccaaacta cccagaaggg acagaagaac
     7021 agtagggaaa ggatgaagag gattgaagag ggtataaaag aactggggtc ccaaatcctt
     7081 aaggaacacc cagttgaaaa cacccagctt cagaatgaga agctctacct gtactacctg
     7141 cagaacggca gggacatgta cgtggatcag gaactggaca tcaatcggct ctccgactac
     7201 gacgtggatg caatcgtgcc ccagtctttt ctcaaagatg attctattga taataaagtg
     7261 ttgacaagat ccgataaaaa tagagggaag agtgataacg tcccctcaga agaagttgtc
     7321 aagaaaatga aaaattattg gcggcagctg ctgaacgcca aactgatcac acaacggaag
     7381 ttcgataatc tgactaaggc tgaacgaggt ggcctgtctg agttggataa agccggcttc
     7441 atcaaaaggc agcttgttga gacacgccag atcaccaagc acgtggccca aattctcgat
     7501 tcacgcatga acaccaagta cgatgaaaat gacaaactga ttcgagaggt gaaagttatt
     7561 actctgaagt ctaagctggt ctcagatttc agaaaggact ttcagtttta taaggtgaga
     7621 gagatcaaca attaccacca tgcgcatgat gcctacctga atgcagtggt aggcactgca
     7681 cttatcaaaa aatatcccaa gcttgaatct gaatttgttt acggagacta taaagtgtac
     7741 gatgttagga aaatgatcgc aaagtctgag caggaaatag gcaaggccac cgctaagtac
     7801 ttcttttaca gcaatattat gaattttttc aagaccgaga ttacactggc caatggagag
     7861 attcggaagc gaccacttat cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag
     7921 ggtagggatt tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa
     7981 aagaccgaag tacagaccgg aggcttctcc aaggaaagta tcctcccgaa aaggaacagc
     8041 gacaagctga tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg attcgattct
     8101 cctacagtcg cttacagtgt actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa
     8161 ctcaaaagcg tcaaggaact gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa
     8221 aaccccatcg actttctcga ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt
     8281 aagcttccca agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt
     8341 gcgggcgagc tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg
     8401 tatctggcca gccactatga aaagctcaaa gggtctcccg aagataatga gcagaagcag
     8461 ctgttcgtgg aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgaattc
     8521 tccaaaagag tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag
     8581 cacagggata agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc
     8641 aacttgggcg cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac
     8701 acctctacaa aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat
     8761 gaaacaagaa tcgacctctc tcagctcggt ggagacagca gggctgacgg gggaggatca
     8821 gggggagggt cccccaaaaa gaaacgcaaa gtgaccggca agaaaaccag cggccaggcc
     8881 ggacaggcct ctcctaagaa aaaaagaaaa gtgggacgcg ctggcggcgg aagcgcccac
     8941 atcgtgatgg tggacgccta caagccgacg aagggaagcg ggtccggtgg aagtggctcc
     9001 ggatctggag gttctggcag cggaggtagc ggcagtggcg ggaaactggg cgatattgaa
     9061 tttattaaag tgaacaaagg gtcgggaagt ggcggcagcg gaagtgggag tggagggagc
     9121 ggttctggcg gttccggcag tggagaggag cttctgagca aaaactatca cctcgaaaac
     9181 gaggttgcgc gactgaagaa aggttccggc tccaacggtg gaagtggctc cggatctgga
     9241 ggttctggca gcggaggtag cggcagtggc gaagagctcc ttagtaagaa ctatcatctg
     9301 gaaaatgagg tagcgcgctt aaagaaaggg tcgggaagtg gcggcagcgg aagtgggagt
     9361 ggagggagcg gttctggcgg ttccggcagt ggagaggagt tgctgtctaa gaactaccac
     9421 ttagaaaacg aagtcgcacg gctaaaaaaa ggttccggct ccggcggctc cggttctgga
     9481 agcgggggct cgggatcagg tggatctgga tcaggagagg aattgctttc caaaaactac
     9541 caccttgaga atgaggtggc caggttaaag aaggggagcg gctcgggggg tagtggatcg
     9601 gggtcgggcg ggtcaggaag cggtggtagc ggatctgggg aggagctgct ctcgaagaat
     9661 taccatttgg agaacgaagt ggcgagacta aagaagggaa gcggtagttc cggttctgga
     9721 agcgggggct cgggatcagg tggatctgga tcaggaggcc tgaacgatat ttttgaagcg
     9781 cagaaaattg aatggcatga aggcggtggt tccggaggag gtagtccgaa gaagaaacgt
     9841 aaggtcggat gagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     9901 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
     9961 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
    10021 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
    10081 cagggctcgc agccaacgtc ggggcggcag gccctgccat agacaacttt gtatacaaaa
    10141 gttgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
    10201 aaacagacta cataatactg taaaacacaa catatccagt cactatgaat caactactta
    10261 gatggtatta gtgacctgta ctgcagggcg gccgcttgta ggctccggtg cccgtcagtg
    10321 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
    10381 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
    10441 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
    10501 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
    10561 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
    10621 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
    10681 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
    10741 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
    10801 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
    10861 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
    10921 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
    10981 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
    11041 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
    11101 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
    11161 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
    11221 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
    11281 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
    11341 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
    11401 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
    11461 tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta tagggagacc
    11521 caagctggct aggtaagctt ggtaccgagc tcggatccgc cagccatgaa ggataacacc
    11581 gtgccactga aattgattgc cctgttagcg aacggtgaat ttcactctgg cgagcagttg
    11641 ggtgaaacgc tgggaatgag ccgggcggct attaataaac acattcagac actgcgtgac
    11701 tggggcgttg atgtctttac cgttccgggt aaaggataca gcctgcctga gcctatccag
    11761 ttacttaatg ctaaacagat attgggtcag ctggatggcg gtagtgtagc cgtgctgcca
    11821 gtgattgact ccacgaatca gtaccttctt gatcgtatcg gagagcttaa atcgggcgat
    11881 gcttgcattg cagaatacca gcaggctggc cgtggtcgcc ggggtcggaa atggttttcg
    11941 ccttttggcg caaacttata tttgtcgatg ttctggcgtc tggaacaagg cccggcggcg
    12001 gcgattggtt taagtctggt tatcggtatc gtgatggcgg aagtattacg caagctgggt
    12061 gcagataaag ttcgtgttaa atggcctaat gacctctatc tgcaggatcg caagctggca
    12121 ggcattctgg tggagctgac tggcaaaact ggcgatgcgg cgcaaatagt cattggagcc
    12181 gggatcaaca tggcaatgcg ccgtgttgaa gagagtgtcg ttaatcaggg gtggatcacg
    12241 ctgcaggaag cggggatcaa tctcgatcgt aatacgttgg cggccatgct aatacgtgaa
    12301 ttacgtgctg cgttggaact cttcgaacaa gaaggattgg caccttatct gtcgcgctgg
    12361 gaaaagctgg ataattttat taatcgccca gtgaaactta tcattggtga taaagaaata
    12421 tttggcattt cacgcggaat agacaaacag ggggctttat tacttgagca ggatggaata
    12481 ataaaaccct ggatgggcgg tgaaatatcc ctgcgtagtg cagaaaaata gaacccagct
    12541 ttcttgtaca aagtggtgat ctagctaaaa cgcggcctcg aatcgaattc cgcccccccc
    12601 cctctccctc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
    12661 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
    12721 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
    12781 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
    12841 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
    12901 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
    12961 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
    13021 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
    13081 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
    13141 cgtggttttc ctttgaaaaa cacgatgata atatgaaaaa gcctgaactc accgcgacgt
    13201 ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg
    13261 agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg
    13321 taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg
    13381 ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt
    13441 gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg
    13501 ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga
    13561 cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt
    13621 tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg
    13681 tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg
    13741 aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc
    13801 gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg
    13861 ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg
    13921 agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg
    13981 gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc
    14041 agggtcgatg cgacgaaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg
    14101 cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa
    14161 accgacgccc cagcactcgt ccgagggcaa aggaatagca cgcaccctcg tggaacagta
    14221 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtaaagcgg
    14281 ccgcgatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
    14341 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
    14401 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
    14461 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
    14521 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
    14581 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
    14641 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
    14701 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcaggaat tcgattaacg
    14761 agagcataat attgatatgt gccaaagttg tttctgactg actaataagt ataatttgtt
    14821 tctattatgt ataggttaag ctaattactt attttataat acaacatgac tgtttttaaa
    14881 gtacaaaata agtttatttt tgtaaaagag agaatgttta aaagttttgt tactttatag
    14941 aagaaatttt gagtttttgt ttttttttaa taaataaata aacataaata aattgtttgt
    15001 tgaatttatt attagtatgt aagtgtaaat ataataaaac ttaatatcta ttcaaattaa
    15061 taaataaacc tcgatataca gaccgataaa acacatgcgt caattttacg catgattatc
    15121 tttaacgtac gtcacaatat gattatcttt ctagggttaa ataatagttt ctaatttttt
    15181 tattattcag cctgctgtcg tgaataccga gctccaattc gccctatagt gagtcgtatt
    15241 acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
    15301 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
    15361 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
    15421 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
    15481 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
    15541 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
    15601 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
    15661 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
    15721 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
    15781 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
    15841 aaatattaac gcttacaatt t
//
