LOCUS       sequence-417406-        9086 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses F74A OsTir1-3X Myc from S. cerevisiae ADH1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9086)
  TITLE     Plasmids for auxin-inducible degron tagging of genes in Candida 
            glabrata
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBV514
FEATURES             Location/Qualifiers
     source          1..9086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..29)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(263..282)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(942..1733,1734..1802))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1565..1584
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1803..1907)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1975..1993
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2031..2053)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2153..2172
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2366..2388
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2380..2397
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2381..2397
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3341..3748
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             3749..4843
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        5607..6008
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             6027..7751
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein, 
                     mutated for use in the AID2 system of inducible degradation
                     (Yesbolatova et al., 2020)"
                     /label=OsTIR1(F74G)
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHAADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             7779..7808
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7815..7844
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7851..7880
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             8019..8048
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             8055..8084
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             8091..8120
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(8867..8883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8867..8883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8880..8902)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8891..8907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8915..8921,8922..8939,8940..8945))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8960..8981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1921 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     1981 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2041 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2101 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     2161 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     2221 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     2281 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     2341 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     2401 ttcgagctcg gtacttaatt aacgtcgctt tgagagaaat cagaagattc caaaagtcta
     2461 ctgaactttt gatcagaaag ttgcctttcc aaagactagt cagagaaatc gcccaagatt
     2521 tcaagaccga tctaagattc caatcctctg ccatcggtgc tctacaggaa tccgttgaag
     2581 cttacttggt ctctttgttc gaagacacca acttggccgc tatccacgct aagcgtgtta
     2641 ccatccaaaa gaaggatatc aagttggcta gaagattgag aggtgaaaga tcctaagcac
     2701 acagggattg ttgtacaatt actttttatg attttagata cacacaaatc ttttctacga
     2761 ttacctttca tttattttcg tttcatgttt tattaatcat tctttttgta tatttcacat
     2821 cgggtggggt ttttatatta aattttaata acataatctt ttaaagtttc tactttctat
     2881 cctgtatatt atttccatat ctatatctat attttcaagc ttgttcacat ccttatcgga
     2941 attgttccag cgtttttgta cagaaaatga tgttatggta acgagctata tcagacctac
     3001 tagtcttata gagctgtaca gagtcttgga aacataaccg tgttgaagaa acatgaaatt
     3061 gcccagtatt cttaacccaa ctgcacagaa caaaaacccc tcgaggagaa cttctagtat
     3121 atctacatac ctaatattat tgccttatta aaaatggaat cccaacaatt acatcaaaat
     3181 ccacattctc ttcaaaatca attgtcctgt acttccttgt tcatgtgtgt tcaaaaacgt
     3241 tatatttata ggataattat actctatttc tcaacaagta attggttgtt tggccgagcg
     3301 gtctaaggcg cctgattcaa gaaatatctt gaccgcagtt aactgtggga atactcaggt
     3361 atcgtaagat gcaagagttc gaatctctta gcaaccatta tttttttcct caacataacg
     3421 agaacacaca ggggcgctat cgcacagaat caaattcgat gactggaaat tttttgttaa
     3481 tttcagaggt cgcctgacgc atataccttt ttcaactgaa aaattgggag aaaaaggaaa
     3541 ggtgagagcg ccggaaccgg cttttcatat agaatagaga agcgttcatg actaaatgct
     3601 tgcatcacaa tacttgaagt tgacaatatt atttaaggac ctattgtttt ttccaatagg
     3661 tggttagcaa tcgtcttact ttctaacttt tcttaccttt tacatttcag caatatatat
     3721 atatatattt caaggatata ccattctaat gtctgcccct aagaagatcg tcgttttgcc
     3781 aggtgaccac gttggtcaag aaatcacagc cgaagccatt aaggttctta aagctatttc
     3841 tgatgttcgt tccaatgtca agttcgattt cgaaaatcat ttaattggtg gtgctgctat
     3901 cgatgctaca ggtgttccac ttccagatga ggcgctggaa gcctccaaga aggctgatgc
     3961 cgttttgtta ggtgctgtgg gtggtcctaa atggggtacc ggtagtgtta gacctgaaca
     4021 aggtttacta aaaatccgta aagaacttca attgtacgcc aacttaagac catgtaactt
     4081 tgcatccgac tctcttttag acttatctcc aatcaagcca caatttgcta aaggtactga
     4141 cttcgttgtt gtcagagaat tagtgggagg tatttacttt ggtaagagaa aggaagacga
     4201 tggtgatggt gtcgcttggg atagtgaaca atacaccgtt ccagaagtgc aaagaatcac
     4261 aagaatggcc gctttcatgg ccctacaaca tgagccacca ttgcctattt ggtccttgga
     4321 taaagctaat gttttggcct cttcaagatt atggagaaaa actgtggagg aaaccatcaa
     4381 gaacgaattc cctacattga aggttcaaca tcaattgatt gattctgccg ccatgatcct
     4441 agttaagaac ccaacccacc taaatggtat tataatcacc agcaacatgt ttggtgatat
     4501 catctccgat gaagcctccg ttatcccagg ttccttgggt ttgttgccat ctgcgtcctt
     4561 ggcctctttg ccagacaaga acaccgcatt tggtttgtac gaaccatgcc acggttctgc
     4621 tccagatttg ccaaagaata aggtcaaccc tatcgccact atcttgtctg ctgcaatgat
     4681 gttgaaattg tcattgaact tgcctgaaga aggtaaggcc attgaagatg cagttaaaaa
     4741 ggttttggat gcaggtatca gaactggtga tttaggtggt tccaacagta ccaccgaagt
     4801 cggtgatgct gtcgccgaag aagttaagaa aatccttgct taaaaagatt ctcttttttt
     4861 atgatatttg tacataaact ttataaatga aattcataat agaaacgaca cgaaattaca
     4921 aaatggaata tgttcatagg gtagacgaaa ctatatacgc aatctacata catttatcaa
     4981 gaaggagaaa aaggaggatg taaaggaata caggtaagca aattgatact aatggctcaa
     5041 cgtgataagg aaaaagaatt gcactttaac attaatattg acaaggagga gggcaccaca
     5101 caaaaagtta ggtgtaacag aaaatcatga aactatgatt cctaatttat atattggagg
     5161 attttctcta aaaaaaaaaa aatacaacaa ataaaaaaca ctcaatgacc tgaccatttg
     5221 atggagttta agtcaatacc ttcttgaacc atttcccata atggtgaaag ttccctcaag
     5281 aattttactc tgtcagaaac ggccttacga cgtagtcgac gggtgtacaa tatggacttc
     5341 ctcttttctg gcaaccaaac ccatacatcg ggattcctat aataccttcg ttggtctccc
     5401 taacatgtag gtggcggagg ggagatatac aatagaacag ataccagaca agacataatg
     5461 ggctaaacaa gactacacca attacactgc ctcattgatg gtggtacata acgaactaat
     5521 actgtagccc tagacttgat agccatcatc atatcgaagt ttcactaccc tttttccatt
     5581 tgccatctat tgaagtaata ataggcgcat gcaacttctt ttcttttttt ttcttttctc
     5641 tctcccccgt tgttgtctca ccatatccgc aatgacaaaa aaatgatgga agacactaaa
     5701 ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt tgttccagag ctgatgaggg
     5761 gtatctcgaa gcacacgaaa ctttttcctt ccttcattca cgcacactac tctctaatga
     5821 gcaacggtat acggccttcc ttccagttac ttgaatttga aataaaaaaa agtttgctgt
     5881 cttgctatca agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc
     5941 tcgttccctt tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac
     6001 aatcaactat ctcatataca actagaatga cgtacttccc ggaggaggtg gtggagcaca
     6061 tcttcagctt cctgccggcg cagcgcgacc gcaacacggt ctcgctcgtc tgcaaggtgt
     6121 ggtacgagat cgagaggctg agccgccgcg gcgtcttcgt gggcaactgc tacgccgtgc
     6181 gcgccggccg cgtcgccgcg cggttcccca acgtgcgggc gctcacggtg aaggggaagc
     6241 cccacgccgc cgacttcaac ctcgtgcccc ccgactgggg cggctacgcg gggccgtgga
     6301 tcgaggcggc cgcgagggga tgccacggcc tggaggagct caggatgaag cggatggtgg
     6361 tgtccgacga gagcctcgag ctgctggctc gctcgttccc gcggttcagg gctcttgttc
     6421 ttatcagctg cgaggggttc agcactgacg ggctagccgc cgtcgcgagc cattgcaagc
     6481 ttctgaggga gttggatttg caggaaaatg aagtggagga tcgagggcct aggtggcttt
     6541 cctgcttccc tgattcctgc acatcacttg tctcattgaa ttttgcctgc atcaaagggg
     6601 aggttaatgc tggttcactg gagagacttg ttagcaggtc cccaaacctg cggagtttga
     6661 ggctgaatcg atctgtatcg gtagatacac ttgcaaagat actactgcgt acccctaact
     6721 tggaggattt ggggacaggg aatttgacag atgacttcca aactgagtcc tactttaagc
     6781 ttaccagtgc tctggagaaa tgcaagatgt tgaggagttt gtctggattc tgggatgctt
     6841 ctcctgtttg cctgtcattt atctaccccc tgtgtgctca actgacagga ttgaacttga
     6901 gctatgcacc cacacttgat gcttctgacc ttacaaaaat gattagccgc tgtgtgaagc
     6961 tccaacgcct ttgggtactg gattgtatct cggacaaagg cttgcaagtg gtggcctcca
     7021 gttgcaaaga cttgcaagaa ctcagggtat ttccatcaga tttctacgta gctggttatt
     7081 ctgcagtgac agaggaggga cttgttgcag tatccttggg ctgtccaaaa ctgaactcac
     7141 tactgtactt ctgtcaccaa atgactaatg ctgcactagt tactgtcgcc aagaactgtc
     7201 caaatttcac acgattcaga ctttgtattc ttgagccagg gaagcctgat gttgtgacaa
     7261 gccaaccatt agatgaaggc tttggagcta ttgttcgtga gtgcaaggga ttacaacgtt
     7321 tgtcaatatc tggtcttctc acagacaaag ttttcatgta tattgggaaa tatgcaaaac
     7381 aacttgagat gctttctata gcatttgctg gtgacagtga taagggtatg atgcatgtta
     7441 tgaatggatg caagaattta aggaaactgg agataagaga tagcccgttt ggtgatgctg
     7501 cactcttggg gaattttgct aggtacgaga caatgcgatc cctttggatg tcatcttgca
     7561 atgtcacgtt aaaggggtgc caagtccttg cgtcaaagat gccgatgctc aatgttgagg
     7621 tcataaatga gcgggatggt agcaatgaaa tggaggaaaa ccatggagat ctgcctaaag
     7681 tggagaaatt atatgtgtac cgcacaactg ctggggcgag ggatgatgca ccaaattttg
     7741 ttaaaatcct agtcgactcc ggttctgctg ctagtggtga acaaaagttg atttctgaag
     7801 aagatttgaa cggtgaacaa aagctaatct ccgaggaaga cttgaacggt gaacaaaaat
     7861 taatctcaga agaagacttg aacggatcca ctagctaaaa agtggaacga tcattcaaga
     7921 gatccgtctc aatgaggttt tacaaacaat cctataatac atatgatgca ccaaattttg
     7981 ttaaaatcct agtcgactcc ggttctgctg ctagtggtga acaaaagttg atttctgaag
     8041 aagatttgaa cggtgaacaa aagctaatct ccgaggaaga cttgaacggt gaacaaaaat
     8101 taatctcaga agaagacttg aacggatcca ctagctaaaa agtggaacga tcattcaaga
     8161 gatccgtctc aatgaggttt tacaaacaat cctataatac atatatgcca cttagatata
     8221 ctttgaatac tgaatatact gactcattat attctttaat tgtagtttct caaaaatatt
     8281 catacaggtt agctatgaat tcatgctata atttccattt tgttcatgta agatacacat
     8341 agggataaaa tgtgataact aatcagccaa cttccgggta ccgtttatat tatgcgtact
     8401 tgttatcatg cgttgcgtta ttcaccctct ttttaaatcg tcagtcgaat tcgtaaactt
     8461 cttatataat atcaatattg ataacatctg tttattaatc aaaacattcg ataagtcgga
     8521 attcattcaa tgcataaaaa tctgtcaata ctgaggaaca actggagttt cagtaaaaag
     8581 tcttgcaaag catgccaagt tcagtgactg tcttacacat attaccacag aagtatcttc
     8641 accaaattta gtcaattaat ataagtggat gtaattgtta aatagtattt caggagcaac
     8701 atatgcgatt cgaatcttca catccaaata gcctctcatt cacggcagta agaaaatgtg
     8761 ctatttgcct cagtagagat gtatagtcta cataaaccga atacttgata tagaccgaca
     8821 tccctctaga gtcgacctgc aggcatgcaa gcttggcgta atcatggtca tagctgtttc
     8881 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8941 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     9001 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     9061 ggagaggcgg tttgcgtatt gggcgc
//
