LOCUS       sequence-417408-       10340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SNG-1::CRY2olig(535) in cholinergic motor neurons of 
            C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Vettkotter D, Schneider M, Goulden BD, Dill H, Liewald J, Zeiler S, 
            Guldan J, Ates YA, Watanabe S, Gottschalk A
  TITLE     Rapid and reversible optogenetic silencing of synaptic transmission 
            by clustering of synaptic vesicles.
  JOURNAL   Nat Commun. 2022 Dec 19;13(1):7827. doi: 10.1038/s41467-022-35324-z.
  PUBMED    36535932
REFERENCE   2  (bases 1 to 10340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-19"; volume: "13(1):7827. doi"; pages: " 
            10.1038/s41467-022-35324-z"
COMMENT     Sequence Label: pDV06 [punc-17::SNG-1::CRY2(535)]
FEATURES             Location/Qualifiers
     source          1..10340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5172..6665
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTRGAQIMIGAA"
     primer_bind     complement(7726..7748)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7848..7867
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7945..7963)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8031..8135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8136..8204,8205..8996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8354..8373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9167..9755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9656..9675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9909..9926
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10043..10064
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10079..10084,10085..10102,10103..10109)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10117..10133
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10122..10144
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10141..10157
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10141..10157
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     regulatory      10304..10313
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cctccgaaat gtgtagtcgt catgacaaag tggtgacact ggattacggt aggctccgcc
       61 cacttcttgg aaaagtgacg tgggctcggt gagccctaaa gcttttggtt ttcacaattt
      121 tctggttttt gggatttttg cgggaatttt gagaggagaa agttgggaga gaagatattg
      181 gaatggtccc cggggagctg tacatttata catctagact gaagctgggg tattgcaaaa
      241 agagaaaagc ctatcacttt ctttccatcc tgcatacttg ccaaaaagtt ttggctgagc
      301 ttatttttaa aattggaatt ttttggaatt ttcagaaaag aaaaagtttt ttcttttttt
      361 ttaatttaaa aaatttttgc tatttattgc atggtactac agaaaaactt gaatgaaata
      421 aaaaatttgg aaaattcaag aaaacttttt ttttcatagt ttatttattc gagttttaat
      481 gtggctttta ttttgttcgg tttttgtttt aaaaagattt tatacaaaat ttcaagaatt
      541 tacaattttt ccgttgtaaa aaatccaaaa aaaaatccaa aaaaatagtt cagacctttt
      601 tttaaatttt aaatttaaaa attcaatacc aaaaaattaa aaaaaattag tttttccgag
      661 aataaaggaa aaatgttgaa aaaatattat atataaaatt tccagaaaat gtgaaaactc
      721 caaaaaactg cagacttttc cccaaactag ctattttttg aattcattac ctttaaaggt
      781 ggagtaccga aatttaagac tttgcttttt tagacccaaa atggtccaaa actaccaaat
      841 ttcgtaatga gacgttctga aaatttttca aaaaaatgtt atgggagctc aaagtttttg
      901 aaaaaatggt atatttttag ctaattttaa ccacttcttg gcgtcgcagc ggttggaact
      961 caattttaat taaattatca tccttcaatg tccttcaatc tttgcaattt caatcgacca
     1021 tacgcgttca gagcattttt ttttttcgtt tttcctcccg tccagcacat atttaaatat
     1081 tctctctccc cctcccctgg ttcctaaaaa cttgggaaag gggaatttcg aaggaggtgg
     1141 aagcttcttc ttctccccga gcacacacac acatcctctc ttcaaaatct ttaagaaata
     1201 gtgcgtgtga atgactagac gctgaagcta acttggtttc cgcgaacaat cccaatcagt
     1261 gcctgtgccg aggcttcgga gaagaagaga ttgacgtcgt ctttttcttt ttttgtgaaa
     1321 cagaataaaa aagaatacga aatggacttt ttcacacatt ttcgttactt ttccagaaaa
     1381 aaacgaagct ctagccatgg aagtttgaat tgaaaaccta aaccccgatg tgtattcatt
     1441 tgagcttaag cctaagccta attctacgcc taagcataag cctaagccta tgttcaagcc
     1501 taagccaaat tgtagaacgt taccaaaaga tcaaacctat ttttcctaca gaaaaggtct
     1561 aatgataact gttaagcgag agacaaggag agatatatat caaaaactag cccaaactgt
     1621 gattaacatc tattaactga cctcaaaaaa aaaaatagta gagattaaat actgctcaaa
     1681 aagtttcttg gaaaagtaaa ttgttaggtt tctggggcct gtaaaactgg accgagtttt
     1741 gaagaatttt tttaactttt caaagtttgg cgtcactgaa agctttatat tgtacatgtt
     1801 ccaccaattt tcttttgtat ccgttttttc tgtctcaaaa cggagctcga gcccatgacg
     1861 tgtggatacc gtgagaatcc gcccaattcc ttcacatcga tctcatattt ctttcccagt
     1921 ttatattgaa ttcccgttga attgcggctt tgcaggggag aagatgtgcg tctttatatt
     1981 tttttttcta tccttacccg ccgtgaatcc ggctcgaatt gagccaattt ttatgaatag
     2041 tagagctaat tgacttcgtc tccctagaat tgatgaggta aagctcgtgg ggagatatgg
     2101 agatttttcg attttttttc cagtaatttt cagttcaaaa tttgagcttt tgagcaggag
     2161 ttttgagatt tgtgccaaaa tttcctatta ttttttcaaa tatttttgct tttgtaaaaa
     2221 aggtgtagta gaagttttgt agtgccacta tttttttttt gaaaattatg ttttggcgga
     2281 aagttcttta aaaaagttaa tcaaaatttt tggcgcttta gtcaaattaa ttttctggca
     2341 aaaatattag taattttggc gtgagttcaa ttttcccatt cttaaaattt cacacaatta
     2401 agaattttaa gatttgggtt tttttttcaa caaaaaatca aatatttaca tgaaaattcg
     2461 gcgggaaata aaaaaaaata ttccgattca atgtgtgaat aactttttgc ggggaattca
     2521 aatttgcatt atttaaactt tttttaagct cgaaaaattg aaattcttaa ataaataaat
     2581 aaaattttcc tcgaattttg aatgtttcga ttttttagcg ttattttttc aaaatttcca
     2641 ctaaaactct agttctaaag ttctaaaact ttaaaaactt cctaatgttg tggtcttgtg
     2701 tttttttggc agcgatttcc aaatttctga aattccaaca aaaaaaccac tacgtcagtt
     2761 tgcatatcca accccccccc cccccccccc ccctctgagc caatcaatca tgtgcatttt
     2821 gaattggcgc caaggcaatc ttcgtaaaaa ataacaatag agtcatccct cagaacagaa
     2881 aagaaacccg cccgggggct caaatacaca tgtcattttt cttttttcgc ctgattttta
     2941 gcctaaatta gtgtttagca attttttaag gtaactcggt ggagtcattt gaacttttta
     3001 aactaaaatt tcagaaaaaa ttcttttttt ttgtcaaaaa ataattttcc cgccggaatt
     3061 tttcaaaaaa ataattttta ttttattttt caagttcctt gtttcctctt tattttttta
     3121 ttttgccccc aaaagtttta cactaaaata tttaattttc aggtaattca tcgaaagtct
     3181 ttctattttc cgcatctctt gttcaaagga ttggccaaag gacccaaagg tatgtttcga
     3241 atgatactaa cataacatag aacattttca ggaggaccct tggctagcac cggtgaaatg
     3301 gagaacgtgc gtgcttatgg tgccggacta gctggcgcca atttcgacaa gaacacattt
     3361 ttcaaaaaac caacggtttt gttcaggtgt gctgcattgg tgagttattt tttctgtctt
     3421 tgtggcaaat aatagacgta aaaagtattt ctaaaaaaca acgaaaaaca aaattgttct
     3481 gcctatactt tttgaatttg aaataaatat ttttcagctc ttcgggctca tactttggta
     3541 ctcggtgcca aaaggtggtt ggcacaaacc aagcgacgcc attcatccgg tatcaaatta
     3601 cccacccaga gacgaatttg gggaagaaaa attattattt ttgacaggtg tgcctttatg
     3661 gaaggagctc gtcaacatgc tcgttcgcca ccgccgttgg cttttttgcc gtctgtggag
     3721 ccattgttct aattgttctt gacgccaaga tggaccaaat cagttcggtg ccaacaagaa
     3781 ggagagctgt cctagcagat ttagttgtat cgggtattta atttgtgtgt ggaaaatttg
     3841 aaagtaagag aatttcagca attttcactg ccatattttt gatcggtttt ttcacattct
     3901 ggtccaaatt atccgccttc gaagtcgacg aagacgacga aaatccaata aagacaaaca
     3961 acgcgaaatt cggaattctc agtgctcttc tctccttttt ggcttgggtg cgtcatgaaa
     4021 aaagtataga ggaataggga aaacggagag gcatctgtta tcttttgaca gaatcttgag
     4081 ttgaaaagca acctttttgg ccactgttat cgagtgttac acatcgtctt ttaagtgtca
     4141 acgtgaaagg ttggagagaa tttagtgaaa tatgatataa tttagtccat cagaaagaga
     4201 tttgatactc aagcggtttt cagtagagca tcaaatagag aatacttgca aatcaatttt
     4261 gcacatttta gggaggtgcc gcatttttcg catggcgtcg ttacgaagaa ggaaaccaag
     4321 caactcatga gcccaattac gatgaacatt ttggacaagt gtcgacagac gtgcaagatg
     4381 gttatggata cggaggagac tcaaccggtt agaataattt taaacaaata gagaaaaaac
     4441 tagttgaaaa ttcttatgaa aatcggatct caaattcagt ttgaaatttc aaaattctgt
     4501 gcagaacaca actactcaat tttttagttc accacttttt cattgtattc ttatcacatt
     4561 ttgttcaaca aatctttaga aaaacgtttt acacgtatta tataattttt aagtttatga
     4621 ctcttgagat attacatgtt aaaattgagc aaaatagcgt agtactagta gaacaaaaaa
     4681 ttgatatatc aaatataccg gtgaaaccac aaatacttca aactttgtta gaagaataca
     4741 caaaaataat atttacaata aacttatctg aaacttccca ggaagtttgt tcactttttc
     4801 aaaaaattat atataagcta gttgaaactt gttttacaat ttaaaaaaaa gcaatttttt
     4861 tgagttttct ctggaagaaa tatttttcat aagtgaatat atgattttag tggttttgag
     4921 attttctgca ttgaaaaaat tcatttttct taacattttt tagcaaaaac ttttaaaaaa
     4981 atcacacgtt gtttttttca ggaatcggac atgttggcgc acctccacct caatcctcat
     5041 accaatctgg tgctgcacca caaaccatgc aacaaccacc atcaaaccca tacactcagt
     5101 cggaaggata tggttatggt ggcggagggt ctggtggcgg agggtccggt ggcggagggt
     5161 caggggtacc gatgaagatg gacaaaaaga ctatagtttg gtttagaaga gacctaagga
     5221 ttgaggataa tcctgcatta gcagcagctg ctcacgaagg atctgttttt cctgtcttca
     5281 tttggtgtcc tgaagaagaa ggacagtttt atcctggaag agcttcaaga tggtggatga
     5341 aacaatcact tgctcactta tctcaatcct tgaaggctct tggatctgac ctcactttaa
     5401 tcaaaaccca caacacgatt tcagcgatct tggattgtat ccgcgttacc ggtgctacaa
     5461 aagtcgtctt taaccacctc tatgatcctg tttcgttagt tcgggaccat accgtaaagg
     5521 agaagctggt ggaacgtggg atctctgtgc aaagctacaa tggagatcta ttgtatgaac
     5581 cgtgggagat atactgcgaa aagggcaaac cttttacgag tttcaattct tactggaaga
     5641 aatgcttaga tatgtcgatt gaatccgtta tgcttcctcc tccttggcgg ttgatgccaa
     5701 taactgcagc ggctgaagcg atttgggcgt gttcgattga agaactaggg ctggagaatg
     5761 aggccgagaa accgagcaat gcgttgttaa ctagagcttg gtctccagga tggagcaatg
     5821 ctgataagtt actaaatgag ttcatcgaga agcagttgat agattatgca aagaacagca
     5881 agaaagttgt tgggaattct acttcactac tttctccgta tctccatttc ggggaaataa
     5941 gcgtcagaca cgttttccag tgtgcccgga tgaaacaaat tatatgggca agagataaga
     6001 acagtgaagg agaagaaagt gcagatcttt ttcttagggg aatcggttta agagagtatt
     6061 ctcggtatat atgtttcaac ttcccgttta ctcacgagca atcgttgttg agtcatcttc
     6121 ggtttttccc ttgggatgct gatgttgata agttcaaggc ctggagacaa ggcaggaccg
     6181 gttatccgtt ggtggatgcc ggaatgagag agctttgggc taccggatgg atgcataaca
     6241 gaataagagt gattgtttca agctttgctg tgaagtttct tctccttcca tggaaatggg
     6301 gaatgaagta tttctgggat acacttttgg atgctgattt ggaatgtgac atccttggct
     6361 ggcagtatat ctctgggagt atccccgatg gccacgagct tgatcgcttg gacaatcccg
     6421 cgttacaagg cgccaaatat gacccagaag gtgagtacat aaggcaatgg cttcccgagc
     6481 ttgcgagatt gccaactgaa tggatccatc atccatggga cgctccttta accgtactca
     6541 aagcttctgg tgtggaactc ggaacaaact atgcgaaacc cattgtagac atcgacacag
     6601 ctcgtgagct actagctaaa gctatttcaa gaacccgtgg agcacagatc atgatcggag
     6661 cagcacctga tgagattgta gcagatagct tcgaggcctt aggggctaat accattaaag
     6721 aacctggtct ttgcccatct gtgtcttcta atgaccaaca agtaccttcg gctgtttaac
     6781 ctagggcctg caggcccggg agcattcgta gaattccaac tgagcgccgg tcgctaccat
     6841 taccaacttg tctggtgtca aaaataatag gggccgctgt catcagagta agtttaaact
     6901 gagttctact aactaacgag taatatttaa attttcagca tctcgcgccc gtgcctctga
     6961 cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt gctcccaccc
     7021 cctatttttg ttattatcaa aaaacttctc ttaatttctt tgttttttag cttcttttaa
     7081 gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt
     7141 cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat
     7201 gttctgtgta cacttcttat gttttttact tctgataaat ttttttgaaa catcatagaa
     7261 aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc gcaattttta
     7321 tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg
     7381 gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt cctgcttttg
     7441 ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa
     7501 atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct
     7561 cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat ggggtttttg
     7621 ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttccta ctagtcggcc
     7681 gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     7741 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     7801 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     7861 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     7921 ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag gttaatgtca
     7981 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     8041 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8101 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8161 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8221 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8281 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8341 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     8401 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8461 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8521 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8581 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8641 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8701 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8761 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8821 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8881 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8941 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9001 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9061 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9121 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9181 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9241 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     9301 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     9361 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     9421 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9481 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9541 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9601 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9661 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9721 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9781 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     9841 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     9901 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     9961 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    10021 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    10081 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    10141 caggaaacag ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac
    10201 taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg
    10261 atacgctaac aacttggaaa tgaaataagc ttgcatgctc ccggccgcca tggccgcggg
    10321 attacaccaa tcatttctcc
//
