LOCUS       sequence-417418-       11125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  includes two standard UAS cassettes and one UAS cassette designed to
            co-express shRNAs/shRNA clusters and prodein coding sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11125)
  AUTHORS   Teague AG, Quintero M, Karimi Dermani F, Cagan RL, Bangi E
  TITLE     A polycistronic transgene design for combinatorial genetic 
            perturbations from a single transcript in Drosophila.
  JOURNAL   PLoS Genet. 2023 Jun 2;19(6):e1010792. doi: 
            10.1371/journal.pgen.1010792. eCollection 2023 Jun.
  PUBMED    37267433
REFERENCE   2  (bases 1 to 11125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2023-06-2"; volume: "19(6):e1010792. doi"; pages: " 
            10.1371/journal.pgen.1010792. eCollection 2023 Jun"
COMMENT     Sequence Label: pWALIUM-intron 3x UAS attB
FEATURES             Location/Qualifiers
     source          1..11125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    2408..2477
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     polyA_signal    2506..2640
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2912..2932)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          3062..3127
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(3541..3779)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    3803..3897
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3822..3916
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3841..3935
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3860..3954
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3879..3973
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3898..3992
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3917..4011
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    complement(4024..4057)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4076..4210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4482..4502)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4632..4697
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(4843..5081)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    5100..5194
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     polyA_signal    5210..5344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5616..5636)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5766..5831
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(5975..6213)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    6232..6326
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             complement(join(7109..7723,7794..7925,8129..8444,8506..9160,
                     9235..9508,9910..9981))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     10433..10451
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     complement(10484..10503)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10485..10503)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10510..10527)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10510..10526)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10519..10541)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10668..11123
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10755..10774)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10965..10986
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggctagaact agtgtcgaca
     2221 tgcccgccgt gaccgtcgag aacccgctga cgctgccccg cgtatccgca cccgccgacg
     2281 ccgtcgcacg tcccgtgctc accgtgacca ccgcgcccag cggtttcgag ggcgagggct
     2341 tcccggtgcg ccgcgcgttc gccgggatca actaccgcca cctcgacccg ttcatcatga
     2401 tggaccagat gggtgaggtg gagtacgcgc ccggggagcc caagggcacg ccctggcacc
     2461 cgcaccgcgg cttcgagacc gtgacctaca tcgtcgacac tagtggatcc agacatgata
     2521 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2581 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2641 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     2701 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac
     2761 tatacatcaa atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg
     2821 tgtaaatagc aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta
     2881 gcaattctga aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag
     2941 aatgttgaga gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca
     3001 ggttttcctc attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa
     3061 tctaaaatac acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta
     3121 tatttacctt agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga
     3181 agtaaggttc cttcacaaag atcctctaga acgtagcggc cgcctccagg gtctggtagc
     3241 gggtgtacgt ctgacgggtg cgtttcgagt ctttgcaatc tgtaagcata agcaaagaaa
     3301 aaatgggtta gctaatgagt tttataactg cgtattgttt gaaaatatca aaaatgtata
     3361 caaactcgag actagaggta cccatatttt caatattttc aaagtgtatt tttaggggtt
     3421 gttaatcgtg tgtgatgcct acctgatgcc aaagtctcct cgatgtgcga ccaattgaaa
     3481 tcgccggctc cgttcggggc tgtcgaattc ccaatagatc tcaattccca attccctatt
     3541 cagagttctc ttcttgtatt caataattac ttcttggcag atttcagtag ttgcagttga
     3601 tttacttggt tgctggttac ttttaattga ttcactttaa cttgcacttt actgcagatt
     3661 gtttagcttg ttcagctgcg cttgtttatt tgcttagctt tcgcttagcg acgtgttcac
     3721 tttgcttgtt tgaattgaat tgtcgctccg tagacgaagc gcctctattt atactccggc
     3781 gctcgctgtc gacgagtctc cgctcggagg acagtactcc gctcggagga cagtactccg
     3841 ctcggaggac agtactccgc tcggaggaca gtactccgct cggaggacag tactccgctc
     3901 ggaggacagt actccgctcg gaggacagta ctccgctcgg aggacagtac tccgctcgga
     3961 ggacagtact ccgctcggag gacagtactc cgctcggagg acagtactcc gacctgcctg
     4021 cagataactt cgtatagcat acattatacg aagttataag ctggtactgc atgcggatcc
     4081 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     4141 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4201 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     4261 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta
     4321 gtcaaggcac tatacatcaa atatttttcc ataattttct tgtatagcag tgcagctttt
     4381 tcctttgtgg tgtaaatagc aaagcaagca agagttctat tactaaacac agcatgactc
     4441 aaaaaactta gcaattctga aggaaagtcc ttggggtctt ctacctttct cttctttttt
     4501 ggaggagtag aatgttgaga gtcagcagta gcctcatcat cactagatgg catttcttct
     4561 gagcaaaaca ggttttcctc attaaaggca ttccaccact gctcccattc atcagttcca
     4621 taggttggaa tctaaaatac acaaacaatt agaatcagta gtttaacaca ttatacactt
     4681 aaaaatttta tatttacctt agagctttaa atctctgtag gtagtttgtc caattatgtc
     4741 acaccacaga agtaaggttc cttcacaaag atcctctagg tttaaacttc aggcgatcgc
     4801 gtatccgtac gcatatggcg cgccaattcc caattcccta ttcagagttc tcttcttgta
     4861 ttcaataatt acttcttggc agatttcagt agttgcagtt gatttacttg gttgctggtt
     4921 acttttaatt gattcacttt aacttgcact ttactgcaga ttgtttagct tgttcagctg
     4981 cgcttgttta tttgcttagc tttcgcttag cgacgtgttc actttgcttg tttgaattga
     5041 attgtcgctc cgtagacgaa gcgcctctat ttatactccg gcgctcgcta gagtctccgc
     5101 tcggaggaca gtactccgct cggaggacag tactccgctc ggaggacagt actccgctcg
     5161 gaggacagta ctccgctcgg aggacagtac tccgacctgc agaagcttgg atccagacat
     5221 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     5281 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     5341 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     5401 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc tctagtcaag
     5461 gcactataca tcaaatattt ttccataatt ttcttgtata gcagtgcagc tttttccttt
     5521 gtggtgtaaa tagcaaagca agcaagagtt ctattactaa acacagcatg actcaaaaaa
     5581 cttagcaatt ctgaaggaaa gtccttgggg tcttctacct ttctcttctt ttttggagga
     5641 gtagaatgtt gagagtcagc agtagcctca tcatcactag atggcatttc ttctgagcaa
     5701 aacaggtttt cctcattaaa ggcattccac cactgctccc attcatcagt tccataggtt
     5761 ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac acttaaaaat
     5821 tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta tgtcacacca
     5881 cagaagtaag gttccttcac aaagatcctc taggacgtct tcagttaatt aagtatcacc
     5941 ggtcatatgg ccggccaatt cccaattccc tattcagagt tctcttcttg tattcaataa
     6001 ttacttcttg gcagatttca gtagttgcag ttgatttact tggttgctgg ttacttttaa
     6061 ttgattcact ttaacttgca ctttactgca gattgtttag cttgttcagc tgcgcttgtt
     6121 tatttgctta gctttcgctt agcgacgtgt tcactttgct tgtttgaatt gaattgtcgc
     6181 tccgtagacg aagcgcctct atttatactc cggcgctcgc tagagtctcc gctcggagga
     6241 cagtactccg ctcggaggac agtactccgc tcggaggaca gtactccgct cggaggacag
     6301 tactccgctc ggaggacagt actccgacct gcaggctagc ctagttccag tgaaatccaa
     6361 gcattttcta aattaaatgt attcttatta ttatagttgt tatttttgat atatataaac
     6421 aacactatta tgcccaccat ttttttgaga tgcatctaca caaggaacaa acactggatg
     6481 tcactttcag ttcaaattgt aacgctaatc actccgaaca ggtcacaaaa aattacctta
     6541 aaaagtcata atattaaatt agaataaata tagctgtgag ggaaatatat acaaatatat
     6601 tggagcaaat aaattgtaca tacaaatatt tattactaat ttctattgag acgaaatgaa
     6661 ccactcggaa ccatttgagc gaaccgaatc gcgcggaact aacgacagtc gctccaaggt
     6721 cgtcgaacaa aaggtgaatg tgttgcggag agcgggtggg agacagcgaa agagcaacta
     6781 cgaaacgtgg tgtggtggag gtgaattatg aagagggcgc gcgatttgaa aagtatgtat
     6841 ataaaaaata tatcccggtg ttttatgtag cgataaacga gtttttgatg taaggtatgc
     6901 aggtgtgtaa gtcttttggt tagaagacaa atccaaagtc tacttgtggg gatgttcgaa
     6961 ggggaaatac ttgtattcta taggtcatat cttgttttta ttggcacaaa tataattaca
     7021 ttagcttttt gagggggcaa taaacagtaa acacgatggt aataatggta aaaaaaaaaa
     7081 acaagcagtt atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag
     7141 ccagatatgc gagcacccgg aagctcacga tgagaatggc cagacccacg tagtccagcg
     7201 gcagatcggc ggcggagaag ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg
     7261 tggtgttcga cgatgtgcag ctaatttcgc ccggctccac gtccgcccat tggttaatca
     7321 gcagaccctc gttggcgtaa cggaaccatg agaggtacga caaccatttg aggtatactg
     7381 gcaccgagcc cgagttcaag aagaagccgc caaagagcag gaatggtatg ataaccggcg
     7441 gacccacaga cagcgccatc gaggtcgagg agctggcgca ggatattaga tatccgaagg
     7501 acgttgacac attggccacc agagtgacca gcgccaggca gttgaagaag tgcagcactc
     7561 cggcccgcag tccgatcatc ggataggcaa tcgccgtgaa gaccagtggc actgtgagaa
     7621 aaagcggcaa ttcggcaatc gttttgccca gaaagtatgt gtcacagcga taaagtcgac
     7681 ttcgggcctc cctcataaaa actggcagct ctgaggtgaa cacctaaatc gaatcgattc
     7741 attagaaagt tagtaaatta ttgaaatgca aatgtattct aaacatgact tacatttatc
     7801 gtggcaaaga cgttttgaaa ggtcatgttg gtcaggaaga ggaagatggc tccgttgata
     7861 ttcatcacac ccacttgcgt gagttgttgg cccaaaaaga tgaggccaat caagatggca
     7921 accatctgca aattaaaatg ttactcgcat ctcattaata ttcgcgagtt aaatgaaatt
     7981 tatttatctt ctgcaaaact ataaactata catctcattg aaaaaaacta agaagggtgt
     8041 ggaatcaggc aattctatct aaaatctagc gaatttgttt ccaagaattg taagcgttat
     8101 atcatttgtt tccactggaa ccactcaccg ttgtctgaat aagtcgcact tttacgagga
     8161 gtggttcctt gagcaccgac agccaggatc gccacaggac cgcccggaac tgcatgaacc
     8221 aggtggcctt gtaggtgtac ccattctccg gctgctccag tggcttctcc agatttttgg
     8281 tggccaacaa ctgctccata tcccgggcta ctttgctaat ggcaaaattg tcgcatatct
     8341 tggcgatccg atcacgggac tcgatctccc gtccgggcac aacggccaac acctgtacgt
     8401 aaaagtccgc cggattgtag ttggtaggac actgggcacc cacgctggat aggagttgag
     8461 atgttatgta atactagata cccttaataa acacatcgaa ctcactagga aaagaagtcg
     8521 acggcttcgc tgggagtgcc caagaaagct accctgccct cggccatcag aaggatcttg
     8581 tcaaagagct caaacagctc ggaagacggc tgatgaatgg tcaggatgac ggtcttgccc
     8641 ttctgcgaca gcttcttcag cacctggacg acgctgtggg cggtaaagga gtccagtccg
     8701 gaggtgggct catcgcagat cagaagcggc ggatcggtta gagcctcgga ggcgaatgcc
     8761 agacgcttcc tttctccgcc ggacagacct ttcaccctgc cgggcacacc gatgatcgtg
     8821 tgctgacatt tgctgagcga aagctcctgg atcacctgat ccacgcgggc cactcgctgc
     8881 cgataggtca gatgtcgtgg catccgcacc atggcttgga aaatcaggtg ttccctggcc
     8941 gttagggagc cgataaagag gtcatcctgc tggacatagg cgcacctggc ctgcatctcc
     9001 ttggcgtcca caggttggcc attgagcagt cgcatcccgg atggcgatac ttggatgccc
     9061 tgcggcgatc gaaaggcaag ggcattcagc agggtcgtct ttccggcacc ggaactgccc
     9121 atcacggcca aaagttcgcc cggataggcc acgccgcaaa ctgagtttca aattggtaat
     9181 tggacccttt attaagattt cacacagatc agccgactgc gaatagaaac tcaccgttct
     9241 tgagcaaatg tttcctgggc gccggtatgt gtcgctcgtt gcagaatagt ccgcgtgtcc
     9301 ggttgaccag ctgccgccat ccggagcccg gctgattgac cgccccaaag atgtccatat
     9361 tgtgccaggc ataggtgagg ttctcggcta gttggccgct ccctgaaccg gagtcctccg
     9421 gcggactggg tggccggagc gtgccgtagt ttttggcctg cccgaagccc tggttaatgc
     9481 agctctgcga agccgctccg ctgtcaccct gcaatgatag gggatctcaa atatcaacta
     9541 caagcgttat gctcatctaa ccccgaacaa aaagtacccc gaagtatcct acgaagtagg
     9601 tttatacttt tatttatttt ttgtgcatct aggatcagct taaaatatct ggttgttata
     9661 ttttttgtaa aaaagaatat agtcgaaaat gaatgccttt agatgtcttg atcatgatat
     9721 gatctcaaaa attgtcttat atagcgagaa cagctaccag aataatctgt ttcgtgtcac
     9781 tatttgtttg tgcaattgcg gtttgggatt tttgtgggtc gcagttctca cgccgcagac
     9841 aatttgatgt tgcaatcgca gttcctatag atcaagtgaa cttaagatgt atgcacatgt
     9901 actactcaca ttgttcagat gctcggcaga tgggtgtttg ctgcctccgc gaattaatag
     9961 ctcctgatcc tcttggccca ttgccgggat ttttcacact ttcccctgct tacccaccca
    10021 aaaccaatca ccaccccaat cactcaaaaa acaaacaaaa ataagaagcg agaggagttt
    10081 tggcacagca ctttgtgttt aattgatggc gtaaaccgct tggagcttcg tcacgaaacc
    10141 gctgacaaaa tgcaactgaa ggcggacatt gacgctacgt aacgctacaa acggtggcga
    10201 aagagatagc ggacgcagcg gcgaaagaga cggcgatatt tctgtggaca gagaaggagg
    10261 caaacagcgc tgactttgag tggaatgtca ttttgagtga gaggtaatcg aaagaacctg
    10321 gtacatcaaa tacccttgga tcgaagtaaa tttaaaactg atcagataag ttcaatgata
    10381 tccagtgcag taaaaaaaaa aaatgttttt tttatctact ttccgcaaaa atgggtttta
    10441 ttaacttaca tacatactag gggcccggta cgtacccaat tcgccctata gtgagtcgta
    10501 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
    10561 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
    10621 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
    10681 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
    10741 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
    10801 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
    10861 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
    10921 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
    10981 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
    11041 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
    11101 caaaatatta acgcttacaa tttag
//
