LOCUS       sequence-417421-       15417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The T-DNA backbone of vCas12j2 for genome editing in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15417)
  AUTHORS   Liu S, Sretenovic S, Fan T, Cheng Y, Li G, Qi A, Tang X, Xu Y, Guo 
            W, Zhong Z, He Y, Liang Y, Han Q, Zheng X, Gu X, Qi Y, Zhang Y
  TITLE     Hypercompact CRISPR-Cas12j2 (CasPhi) enables genome editing, gene 
            activation, and epigenome editing in plants.
  JOURNAL   Plant Commun. 2022 Sep 20:100453. doi: 10.1016/j.xplc.2022.100453.
  PUBMED    36127876
REFERENCE   2  (bases 1 to 15417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2022 Sep 20:100453. doi: 10.1016/j.xplc.2022.100453."
COMMENT     Sequence Label: pGEL637
FEATURES             Location/Qualifiers
     source          1..15417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    308..448
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(374..393)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     524..546
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      782..976
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1042..2115)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(2544..3173)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4474..4498
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(4519..4767)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(4778..4825)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(7064..7084)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(7099..9096)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     ncRNA           10656..10692
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     ncRNA           11135..11202
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     protein_bind    complement(11222..11246)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        11603..12279
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     12216..12236
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    13369..13543
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    13621..13645
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             14070..14864
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      join(14951..15417,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
      361 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
      421 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgct
      481 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tttccgggag ctgcatgtgt
      541 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg
      601 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc
      661 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga
      721 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag
      781 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga
      841 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta
      901 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat
      961 gtacgtgcta tccacaggaa agaggccttt tcgacctttt tcccctgcta gggcaatttg
     1021 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg
     1081 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg
     1141 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc
     1201 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc
     1261 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg
     1321 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc
     1381 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct
     1441 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg
     1501 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg
     1561 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac
     1621 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga
     1681 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga
     1741 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg
     1801 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc
     1861 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga
     1921 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg
     1981 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc
     2041 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg
     2101 tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg
     2161 caggcaaccc agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc
     2221 acggcgtcgg tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt
     2281 catctactca tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag
     2341 cagctcggta atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc
     2401 cgccggcaac tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt
     2461 tgcagccttg ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct
     2521 cattttctct ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc
     2581 tggacctcgc gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg
     2641 gcagtgcctg ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac
     2701 ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg
     2761 acaaaggccg cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc
     2821 aggtcggcgg tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg
     2881 gctgccttga tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg
     2941 aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca
     3001 acggttagcg gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg
     3061 gaatcgacta acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg
     3121 atggtcgtct tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc
     3181 ccttgcgtat ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc
     3241 tgttttactc aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca
     3301 agctggccgg ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc
     3361 gcacggttga gccgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct
     3421 cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg
     3481 ttcctcttgg cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc
     3541 acggaaggca ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc
     3601 gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc
     3661 ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg
     3721 gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat
     3781 tagggaacgc tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg
     3841 cgtggtggtg tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat
     3901 gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac
     3961 tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg
     4021 cctggtgccg gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc
     4081 ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc
     4141 ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat
     4201 gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt
     4261 aacttaggac ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc
     4321 gactgcacta tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg
     4381 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
     4441 ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa
     4501 cactgatagt ttgagctcct tatctttaat catattccat agtccatacc atagcacata
     4561 cagtagttat atgctgcaga agagatccaa caaaacattc acaatggatt atagaaacat
     4621 ttgtttattc attataatgt gatcttacat tcatttaata ttagaaaaag ccacaaattc
     4681 ataacacaac aagccaagaa aaaaacacaa acttaagcac acaagctttt tatttgacac
     4741 accaaatatt tcatcttcat cttcatattc tagatcactt ctttttctta gcctgtccgg
     4801 cctttttggt ggcagcagga cgcttacttg tttgggatgg ttcctgggct ggagtctgat
     4861 cctccctctc cgccggcggc gccttggact tgctagcctt cttcgttttt ctggccgggg
     4921 ccgtcccttg tgcatcccta ggttcctctc tcttcggcat tgttttgcct gtgagagcca
     4981 cctgagtgag attatgcgta gccacatcaa gatccgcatt acaggtcttc ccgcacgaaa
     5041 ggcattgaaa ggcctcaccg tcccggttcc cgacttcaca atgtccacac tttgggcatg
     5101 tgatggacgt tctttccggc ctcacctcga agacataaaa ggagcgatgc gtccggagat
     5161 cactaaaggc cttgtgcaat ccttgtatga accagcggtt ttctttttta gctgtaaaga
     5221 aattgtccca tccgggcaac cgctttccag aaccatgaaa gaagcgaaca ttgaggtctt
     5281 cgatgaccgg aataacaatt tgacactgag tcctccggcg tgtcttctct ataacgtaat
     5341 taatcgacct tctacagagt tcttcctttc tcctggagag tttaagatac tctggagatg
     5401 tccgcttcaa ggcccaaagt gcttcgttct tcaatttctg cgcttcaact gacagtctcg
     5461 gtttgtaagc gcgtgcccat gtgcggtctt tcctcataac ttccaccgga gctttctttt
     5521 tcgcgccttt tttcggggcg ggtgtgaaaa agacttggtc gcgactaacg ctgttactaa
     5581 gcagggcctc ggagatgaag gtagtattcg acgacatttt atcccaaggg agtctcttag
     5641 gatcaagccc aaaatcagca cacaattgag ttctggcagt ctctttagaa acgccatcca
     5701 gtgccctaac ctcagcctgc tgtgcttctg gcaaggcttc aacactgcga gcgcggagtt
     5761 cctcctcgtt ccggtcccag gcgattctgt aggcactgat gtctttaaga agatcatcgg
     5821 ggagtgtaaa gcgatcgaga ggctcgcact gaagtgcacc attctcctga gtaaccctgc
     5881 tgatcccggc actaattggg ttggtttgcc caaggtctat ggccaccaaa gcgactgtct
     5941 gcgtggctgt caacttatcc agtgtggcct tagtctgctt tcctttcagg gtccacttgc
     6001 gagcgtatgt tccgcatgcg tccagagtat aagtaaaggt gactatgttc cgcctcacat
     6061 caataactgg gtcgccagtg aacagatcga gaagcgcgtt cagactaatg tccttcggcg
     6121 caatagtcct ccaccgtgcg ttcctcaaaa gccctctaac gtcaatgacg acccaatccg
     6181 tgcctatcct cacggtgaca aggagtgcat cttgggcctt ctccttctct gaccgccaat
     6241 acctcctcat tctcttattt tttttaggac tcaatcccgg caccgtaacg gctttgccgg
     6301 ttttaggtga tatcgcagtc ccagcctcgc gctgccactc gggtatatac cctgggcagc
     6361 ctttctgtat atcgcatcta ttcctaacga cgcccaacgg tataggcgcg tttgggtccc
     6421 taacgtagcc agcgtactca ggcggaagga cgatctcatc gcgggggcga taagcctgag
     6481 ggctgatagt ctgataaaca taaaaggaag ggtttattcc gggtggctgc agcaagtgtc
     6541 ccgtctcatt cgttgcaact tcttcttcgc cggaactgcc tgctttttca ttcctcagtt
     6601 gaaccttctt caacacccca tcgtagcgtc ccagtgtatg gtcaaagatc aggttaagac
     6661 cctgaacatg gctatatcca tgattagaca cccctgtagc agcaaaccat gccgcgtgcg
     6721 actcagagga ttttccaggc ttacacgccg ctctttcagt agtgctaagg gcgtagatat
     6781 agcgctgtat ggcttcactt gccttcatga ttggccactc cgcaaaatcc cgggacttcg
     6841 tgaccacatg gcactttgct ggaggttgaa agttcggtgg ttcctcctcg gatttgccct
     6901 ggagatatgc cacgacggct tcctctcctt gagccgccag aatcttcccc cctcttttca
     6961 tgtaggatga tctgaatctc tcgccaggga agtgcttttt caacacttta gagaattcgc
     7021 tttcgacagc cggtttagga gccgcaggca ccccgtgaat accaaccttc cgcttcttct
     7081 taggagccat cctgcaggct gcagaagtaa caccaaacaa cagggtgagc atcgacaaaa
     7141 gaaacagtac caagcaaata aatagcgtat gaaggcaggg ctaaaaaaat ccacatatag
     7201 ctgctgcata tgccatcatc caagtatatc aagatcaaaa taattataaa acatacttgt
     7261 ttattataat agataggtac tcaaggttag agcatatgaa tagatgctgc atatgccatc
     7321 atgtatatgc atcagtaaaa cccacatcaa catgtatacc tatcctagat cgatatttcc
     7381 atccatctta aactcgtaac tatgaagatg tatgacacac acatacagtt ccaaaattaa
     7441 taaatacacc aggtagtttg aaacagtatt ctactccgat cttgaacgaa tgaacgaccg
     7501 cccaaccaca ccacatcatc acaaccaagc gaacaaaaag catctctgta tatgcatcag
     7561 taaaacccgc atcaacatgt atacctatcc tagatcgata tttccatcca tcatcttcaa
     7621 ttcgtaacta tgaatatgta tggcacacac atacagatcc aaaattaata aatccaccag
     7681 gtagtttgaa acagaattaa ttctactccg atcttgaacg accgcccaac cagaccacat
     7741 catcacaacc aagacaaaaa aaagcatgaa aagatgaccc gacaaacaag tgcacggcat
     7801 atattgaaat aaaggaaaag ggcaaaccaa accctatgca acgaaacaaa aaaaatcatg
     7861 aaatcgatcc cgtctgcgga acggctagag ccatcccagg attccccaaa gagaaacact
     7921 ggcaagttag caatcagaac gtgtctgacg tacaggtcgc atccgtgtac gaacgctagt
     7981 agcacggatc taacacaaac acggatctaa cacaaacatg aacagaagta gaactaccgg
     8041 gccctaacca tggaccggaa cgccgatctt gagaaggtag agaggggggg gggggaggac
     8101 gagcggcgta ccttgaagcg gaggtgccga cgggtggatt tgggggagtt ctggttgtgt
     8161 gtgtgtgcgc tccgaacaac acgaggttgg ggaaagaggg tgtggagggg gtgtctattt
     8221 attacggcgg gcgaggaagg gaaagcgaag gagcggtggg aaaggaatcc cccgtagctg
     8281 ccggtgccgt gagaggagga ggaggccgcc tgccgtgccg gctcacgtct gccgctccgc
     8341 cacgcaattt ctggatgccg acagcggagc aagtccaacg gtggagcgga actctcgaga
     8401 ggggtccaga ggcagcgaca gagatgccgt gccgtctgct tcgcttggcc cgacgcgacg
     8461 ctgctggttc gctggttggt gtccgttaga ctcgtcgacg gcgtttaaca ggctggcatt
     8521 atctactcga aacaagaaaa atgtttcctt agttttttta atttcttaaa gggtatttgt
     8581 ttaattttta gtcactttat tttattctat tttatatcta aattattaaa taaaaaaact
     8641 aaaatagagt tttagttttc ttaatttaga ggctaaaata gaataaaata gatgtactaa
     8701 aaaaattagt ctataaaaac cattaaccct aaaccctaaa tggatgtact aataaaatgg
     8761 atgaagtatt atataggtga agctatttgc aaaaaaaaag gagaacacat gcacactaaa
     8821 aagataaaac tgtagagtcc tgttgtcaaa atactcaatt gtcctttaga ccatgtctaa
     8881 ctgttcattt atatgattct ctaaaacact gatattattg tagtactata gattatatta
     8941 ttcgtagagt aaagtttaaa tatatgtata aagatagata aactgcactt caaacaagtg
     9001 tgacaaaaaa aatatgtggt aattttttat aacttagaca tgcaatgctc accttatctc
     9061 aagagagggg cacgaccggg tcacgctgca ctgcaggcgc gcccacatca gtctctgcac
     9121 aaagtgcatc ctgggctgct tcaattataa agccccattc accacatttg ctagatagtc
     9181 gaaaagcacc atcaatattg agcttcaggt atttttggtt gtgttgtggt tggattgatt
     9241 ctaatatata ccaaatcaat ataattcact accaaaatat accatagcca tcacaacttt
     9301 attaattttg gtagcttaag atggtatata taataaccaa ttaacaactg attctaattt
     9361 tactacggcc cagtatctac caatacaaaa caacgagtat gttttcttcc gtcgtaatcg
     9421 tacacagtac aaaaaaacct ggccagcctt tcttgggctg gggctctctt tcgaaaggtc
     9481 acaaaacgta cacggcagta acgccgcttc gctgcgtgtt aacggccacc aaccccgccg
     9541 tgagcaaacg gcatcagctt tccacctcct cgatatctcc gcggcgccgt ctggacccgc
     9601 cccctttccg ttcctttctt tccttctcgc gtttgcgtgg tggggacgga ctccccaaac
     9661 cgcctctccc tctctttatt tgtctatatt ctcactgggc cccacccacc gcacccctgg
     9721 gcccactcac gagtcccccc ctccccacct ataaataccc caccccctcc tcgcctcttc
     9781 ctccatcaat cgaatcccca aaatcgcaga gaaaaaaaaa tctcccctcg aagcgaagcg
     9841 tcgaatcgcc ttctcaaggt atgcgatttt ctgatcctct ccgttcctcg cgtttgattt
     9901 gatttcccgg cctgttcgtg attgtgagat gttgttgtta gtctccgttt tgcgatctgt
     9961 ggtagatttg aacagggtta gatggggttc gcgtggtatg ctggatctgt gattatgagc
    10021 gatgctgttc gtggtccaag tatttattgg ttcggatcta gaagtagaac tgtgctaggg
    10081 ttgtgatttg ttccgatctg ttcaatcagt aggatttagt ctctgttttt ctcgttgatc
    10141 caagtagcag cttcaggtat attttgctta ggttgttttt gattcagtcc ctctagttgc
    10201 atagattcta ctctgttcat gtttaatcta agggctgcgt cttgttgatt agtgattaca
    10261 tagcatagct ttcaggatat tttacttgct tatgcctatc ttatcaactg ttgcacctgt
    10321 aaattctagc ctatgttaat taacctgcct tatgtgctct cgggatagtg ctagtagtta
    10381 ttgaatcagt ttgccgatgg aattctagta gttcatagac ctgcagatta tttttgtgaa
    10441 ctcgagcacg gtgcgtctct ctattttgtt aggtcactgt tggtgttgat aggtacactg
    10501 atgttattgt ggtttagatc gtgtatctaa catattggaa taatttgatt gactgatttc
    10561 tgctgtactt gcttggtatt gttataattt catgttcata gttgctgacc atgcttcggt
    10621 aattgtgtgt gcagatccag atcggatcct cgttgctgat gagtccgtga ggacgaaacg
    10681 agtaagctcg tccaacgatt gcccctcacg aggggacgga gaccgggcgt gtcaataata
    10741 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta
    10801 aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg
    10861 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg
    10921 ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg
    10981 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc
    11041 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc
    11101 gcaaatacgc atactgttat ctggcttggt ctcaggccgg catggtccca gcctcctcgc
    11161 tggcgccggc tgggcaacat gcttcggcat ggcgaatggg acggtacccg gccggaattc
    11221 gacccagctt tcttgtacaa agtggttcga taattcctta attcgaattt ccccgatcgt
    11281 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
    11341 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
    11401 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
    11461 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
    11521 ctactagtcc catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
    11581 tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
    11641 tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg
    11701 gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg
    11761 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
    11821 caaccacgtc ttcaaagcaa gtggattgat gtgaacatgg tggagcacga cactctcgtc
    11881 tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa
    11941 caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
    12001 aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag
    12061 gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg
    12121 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat
    12181 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct
    12241 atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtc tctctctaca
    12301 aatctatctc tcctaggatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
    12361 tgatcgaaaa gttcgacagc gtgtccgacc tgatgcagct ctcggagggc gaagaatctc
    12421 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
    12481 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
    12541 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
    12601 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
    12661 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
    12721 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
    12781 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
    12841 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
    12901 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
    12961 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
    13021 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
    13081 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
    13141 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
    13201 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
    13261 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
    13321 ctcgtccggg atcttggagg tgattaatta acgatcgaca agctctattt tctccataat
    13381 aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc gctcatgtgt
    13441 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
    13501 tttctaattc ctaaaaccaa aatccagtac taaaatccag atcccccgaa ttaattcggc
    13561 gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt
    13621 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
    13681 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
    13741 cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa
    13801 gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga
    13861 cagagcgttg ctgcctgtga tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta
    13921 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
    13981 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
    14041 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
    14101 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
    14161 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
    14221 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
    14281 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
    14341 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
    14401 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
    14461 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
    14521 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
    14581 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
    14641 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
    14701 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
    14761 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
    14821 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gaatgcatga
    14881 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    14941 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    15001 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    15061 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
    15121 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    15181 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    15241 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    15301 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    15361 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggag
//
