LOCUS       sequence-417422-       16963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The T-DNA backbone of Cas12j2 for transcriptional activation in 
            rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16963)
  AUTHORS   Liu S, Sretenovic S, Fan T, Cheng Y, Li G, Qi A, Tang X, Xu Y, Guo 
            W, Zhong Z, He Y, Liang Y, Han Q, Zheng X, Gu X, Qi Y, Zhang Y
  TITLE     Hypercompact CRISPR-Cas12j2 (CasPhi) enables genome editing, gene 
            activation, and epigenome editing in plants.
  JOURNAL   Plant Commun. 2022 Sep 20:100453. doi: 10.1016/j.xplc.2022.100453.
  PUBMED    36127876
REFERENCE   2  (bases 1 to 16963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2022 Sep 20:100453. doi: 10.1016/j.xplc.2022.100453."
COMMENT     Sequence Label: pGEL636
FEATURES             Location/Qualifiers
     source          1..16963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(554..1348)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1773..1797
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1875..2049
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(3139..3815)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(3182..3202)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    4172..4196
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     ncRNA           complement(4216..4283)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(4726..4762)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     promoter        6322..8320
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             8335..8355
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10648..10695
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             10726..10746
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10759..10779
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10792..10812
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11875..12024
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             12037..12186
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     terminator      12197..12445
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    12466..12490
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             13791..14420
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             14849..15922
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      15988..16182
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(16418..16440)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    16516..16656
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     16571..16590
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(16671..16688)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(16842..16963,1..467))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16922..16941)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
       61 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      121 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      181 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      241 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      301 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
      361 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      421 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
      481 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
      541 tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
      601 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
      661 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
      721 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
      781 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
      841 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
      901 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
      961 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
     1021 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
     1081 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
     1141 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
     1201 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
     1261 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
     1321 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
     1381 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
     1441 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
     1501 catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca
     1561 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
     1621 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct
     1681 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
     1741 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
     1801 ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc
     1861 gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt
     1921 tattgataga agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca
     1981 catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc acacattatt
     2041 atggagaaaa tagagcttgt cgatcgttaa ttaatcacct ccaagatccc ggacgagtgc
     2101 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
     2161 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
     2221 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
     2281 gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg
     2341 gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga
     2401 agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
     2461 ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga
     2521 ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga
     2581 cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
     2641 tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
     2701 cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg
     2761 gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac
     2821 ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa
     2881 tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
     2941 cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
     3001 attcttcgcc ctccgagagc tgcatcaggt cggacacgct gtcgaacttt tcgatcagaa
     3061 acttctcgac agacgtcgcg gtgagttcag gctttttcat cctaggagag atagatttgt
     3121 agagagagac tggtgatttc agcgtgtcct ctccaaatga aatgaacttc cttatataga
     3181 ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc
     3241 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
     3301 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
     3361 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
     3421 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
     3481 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
     3541 gagagtgtcg tgctccacca tgttcacatc aatccacttg ctttgaagac gtggttggaa
     3601 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
     3661 cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac
     3721 cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga
     3781 ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg
     3841 tatctttgat attcttggag tagacgagag tgtcgtgctc caccatggga ctagtagtaa
     3901 catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat
     3961 cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt
     4021 catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat
     4081 catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg
     4141 atcggggaaa ttcgaattaa ggaattatcg aaccactttg tacaagaaag ctgggtcgaa
     4201 ttccggccgg gtaccgtccc attcgccatg ccgaagcatg ttgcccagcc ggcgccagcg
     4261 aggaggctgg gaccatgccg gcctgagacc aagccagata acagtatgcg tatttgcgcg
     4321 ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag
     4381 gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa
     4441 ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt
     4501 cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg
     4561 tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa
     4621 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
     4681 tattgacacg cccggtctcc gtcccctcgt gaggggcaat cgttggacga gcttactcgt
     4741 ttcgtcctca cggactcatc agcaacgagg atccgatctg gatctgcaca cacaattacc
     4801 gaagcatggt cagcaactat gaacatgaaa ttataacaat accaagcaag tacagcagaa
     4861 atcagtcaat caaattattc caatatgtta gatacacgat ctaaaccaca ataacatcag
     4921 tgtacctatc aacaccaaca gtgacctaac aaaatagaga gacgcaccgt gctcgagttc
     4981 acaaaaataa tctgcaggtc tatgaactac tagaattcca tcggcaaact gattcaataa
     5041 ctactagcac tatcccgaga gcacataagg caggttaatt aacataggct agaatttaca
     5101 ggtgcaacag ttgataagat aggcataagc aagtaaaata tcctgaaagc tatgctatgt
     5161 aatcactaat caacaagacg cagcccttag attaaacatg aacagagtag aatctatgca
     5221 actagaggga ctgaatcaaa aacaacctaa gcaaaatata cctgaagctg ctacttggat
     5281 caacgagaaa aacagagact aaatcctact gattgaacag atcggaacaa atcacaaccc
     5341 tagcacagtt ctacttctag atccgaacca ataaatactt ggaccacgaa cagcatcgct
     5401 cataatcaca gatccagcat accacgcgaa ccccatctaa ccctgttcaa atctaccaca
     5461 gatcgcaaaa cggagactaa caacaacatc tcacaatcac gaacaggccg ggaaatcaaa
     5521 tcaaacgcga ggaacggaga ggatcagaaa atcgcatacc ttgagaaggc gattcgacgc
     5581 ttcgcttcga ggggagattt ttttttctct gcgattttgg ggattcgatt gatggaggaa
     5641 gaggcgagga gggggtgggg tatttatagg tggggagggg gggactcgtg agtgggccca
     5701 ggggtgcggt gggtggggcc cagtgagaat atagacaaat aaagagaggg agaggcggtt
     5761 tggggagtcc gtccccacca cgcaaacgcg agaaggaaag aaaggaacgg aaagggggcg
     5821 ggtccagacg gcgccgcgga gatatcgagg aggtggaaag ctgatgccgt ttgctcacgg
     5881 cggggttggt ggccgttaac acgcagcgaa gcggcgttac tgccgtgtac gttttgtgac
     5941 ctttcgaaag agagccccag cccaagaaag gctggccagg tttttttgta ctgtgtacga
     6001 ttacgacgga agaaaacata ctcgttgttt tgtattggta gatactgggc cgtagtaaaa
     6061 ttagaatcag ttgttaattg gttattatat ataccatctt aagctaccaa aattaataaa
     6121 gttgtgatgg ctatggtata ttttggtagt gaattatatt gatttggtat atattagaat
     6181 caatccaacc acaacacaac caaaaatacc tgaagctcaa tattgatggt gcttttcgac
     6241 tatctagcaa atgtggtgaa tggggcttta taattgaagc agcccaggat gcactttgtg
     6301 cagagactga tgtgggcgcg cctgcagtgc agcgtgaccc ggtcgtgccc ctctcttgag
     6361 ataaggtgag cattgcatgt ctaagttata aaaaattacc acatattttt tttgtcacac
     6421 ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact ctacgaataa
     6481 tataatctat agtactacaa taatatcagt gttttagaga atcatataaa tgaacagtta
     6541 gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt ttatcttttt
     6601 agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat acttcatcca
     6661 ttttattagt acatccattt agggtttagg gttaatggtt tttatagact aattttttta
     6721 gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact ctattttagt
     6781 ttttttattt aataatttag atataaaata gaataaaata aagtgactaa aaattaaaca
     6841 aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg agtagataat
     6901 gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa ccagcagcgt
     6961 cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct
     7021 cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg
     7081 gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag
     7141 ctacggggga ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa
     7201 tagacacccc ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca
     7261 caaccagaac tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc
     7321 ctcccccccc ccccctctct accttctcaa gatcggcgtt ccggtccatg gttagggccc
     7381 ggtagttcta cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctac
     7441 tagcgttcgt acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag
     7501 tgtttctctt tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatttca
     7561 tgattttttt tgtttcgttg catagggttt ggtttgccct tttcctttat ttcaatatat
     7621 gccgtgcact tgtttgtcgg gtcatctttt catgcttttt tttgtcttgg ttgtgatgat
     7681 gtggtctggt tgggcggtcg ttcaagatcg gagtagaatt aattctgttt caaactacct
     7741 ggtggattta ttaattttgg atctgtatgt gtgtgccata catattcata gttacgaatt
     7801 gaagatgatg gatggaaata tcgatctagg ataggtatac atgttgatgc gggttttact
     7861 gatgcatata cagagatgct ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg
     7921 gtcgttcatt cgttcaagat cggagtagaa tactgtttca aactacctgg tgtatttatt
     7981 aattttggaa ctgtatgtgt gtgtcataca tcttcatagt tacgagttta agatggatgg
     8041 aaatatcgat ctaggatagg tatacatgtt gatgtgggtt ttactgatgc atatacatga
     8101 tggcatatgc agcatctatt catatgctct aaccttgagt acctatctat tataataaac
     8161 aagtatgttt tataattatt ttgatcttga tatacttgga tgatggcata tgcagcagct
     8221 atatgtggat ttttttagcc ctgccttcat acgctattta tttgcttggt actgtttctt
     8281 ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag cctgcaggat ggctcctaag
     8341 aagaagcgga aggttggtat tcacggggtg cctgcggctc ctaaaccggc tgtcgaaagc
     8401 gaattctcta aagtgttgaa aaagcacttc cctggcgaga gattcagatc atcctacatg
     8461 aaaagagggg ggaagattct ggcggctcaa ggagaggaag ccgtcgtggc atatctccag
     8521 ggcaaatccg aggaggaacc accgaacttt caacctccag caaagtgcca tgtggtcacg
     8581 aagtcccggg attttgcgga gtggccaatc atgaaggcaa gtgaagccat acagcgctat
     8641 atctacgccc ttagcactac tgaaagagcg gcgtgtaagc ctggaaaatc ctctgagtcg
     8701 cacgcggcat ggtttgctgc tacaggggtg tctaatcatg gatatagcca tgttcagggt
     8761 cttaacctga tctttgacca tacactggga cgctacgatg gggtgttgaa gaaggttcaa
     8821 ctgaggaatg aaaaagcaag agcgcgcctt gagagcatca acgcgagccg cgctgacgaa
     8881 ggactcccgg aaatcaaggc agaagaagaa gaagttgcaa cgaatgagac gggacacttg
     8941 ctgcagccac ccggaataaa cccttccttt tatgtttatc agactatcag ccctcaggct
     9001 tatcgccccc gcgatgagat cgtccttccg cctgagtacg ctggctacgt tagggaccca
     9061 aacgcgccta taccgttggg cgtcgttagg aatagatgcg atatacagaa aggctgccca
     9121 gggtatatac ccgagtggca gcgcgaggct gggactgcga tatcacctaa aaccggcaaa
     9181 gccgttacgg tgccgggatt gagtcctaaa aaaaataaga gaatgaggag gtattggcgg
     9241 tcagagaagg agaaggccca agatgcactc cttgtcaccg tgaggatagg cacggattgg
     9301 gtcgtcattg acgttagagg gcttttgagg aacgcacggt ggaggactat tgcgccgaag
     9361 gacattagtc tgaacgcgct tctcgatctg ttcactggcg acccagttat tgatgtgagg
     9421 cggaacatag tcacctttac ttatactctg gacgcatgcg gaacatacgc tcgcaagtgg
     9481 accctgaaag gaaagcagac taaggccaca ctggataagt tgacagccac gcagacagtc
     9541 gctttggtgg ccatagacct tgggcaaacc aacccaatta gtgccgggat cagcagggtt
     9601 actcaggaga atggtgcact tcagtgcgag cctctcgatc gctttacact ccccgatgat
     9661 cttcttaaag acatcagtgc ctacagaatc gcctgggacc ggaacgagga ggaactccgc
     9721 gctcgcagtg ttgaagcctt gccagaagca cagcaggctg aggttagggc actggatggc
     9781 gtttctaaag agactgccag aactcaattg tgtgctgatt ttgggcttga tcctaagaga
     9841 ctcccttggg ataaaatgtc gtcgaatact accttcatct ccgaggccct gcttagtaac
     9901 agcgttagtc gcgaccaagt ctttttcaca cccgccccga aaaaaggcgc gaaaaagaaa
     9961 gctccggtgg aagttatgag gaaagaccgc acatgggcac gcgcttacaa accgagactg
    10021 tcagttgaag cgcagaaatt gaagaacgaa gcactttggg ccttgaagcg gacatctcca
    10081 gagtatctta aactctccag gagaaaggaa gaactctgta gaaggtcgat taattacgtt
    10141 atagagaaga cacgccggag gactcagtgt caaattgtta ttccggtcat cgaagacctc
    10201 aatgttcgct tctttcatgg ttctggaaag cggttgcccg gatgggacaa tttctttaca
    10261 gctaaaaaag aaaaccgctg gttcatacaa ggattgcaca aggcctttag tgatctccgg
    10321 acgcatcgct ccttttatgt cttcgaggtg aggccggaaa gaacgtccat cacatgccca
    10381 aagtgtggac attgtgaagt cgggaaccgg gacggtgagg cctttcaatg cctttcgtgc
    10441 gggaagacct gtaatgcgga tcttgatgtg gctacgcata atctcactca ggtggctctc
    10501 acaggcaaaa caatgccgaa gagagaggaa cctagggatg cacaagggac ggccccggcc
    10561 agaaaaacga agaaggctag caagtccaag gcgccgccgg cggagaggga ggatcagact
    10621 ccagcccagg aaccatccca aacaagtaag cgtcctgctg ccaccaaaaa ggccggacag
    10681 gctaagaaaa agaagggcag ttccggcggg tcctcaaggg ctgatcctaa gaagaagagg
    10741 aaggtttcta gggcagatcc aaagaagaag cggaaggtgt ctagagctga tccgaagaag
    10801 aagcgcaagg tgcctagggg aggatctggt ggtcttttgg atccaggaac ccctatggat
    10861 gctgaccttg ttgcttcttc caccgttgtc tgggagcagg atgccgaccc tttcgctggt
    10921 accgcagacg acttcccagc ttttaacgag gaagagcttg cttggctcat ggaattgttg
    10981 ccacagggag gttctggtgg attgttggat cctggaactc caatggacgc tgatcttgtt
    11041 gcttcctcta ctgtcgtttg ggagcaagac gctgaccctt tcgctggaac cgctgacgat
    11101 ttcccagcct ttaacgaaga ggaattggcc tggcttatgg agttgcttcc tcaggctagg
    11161 ggaggatctg gtggtctttt ggatccagga acccctatgg atgctgacct tgttgcttct
    11221 tccaccgttg tctgggagca ggatgccgac cctttcgctg gtaccgcaga cgacttccca
    11281 gcttttaacg aggaagagct tgcttggctc atggaattgt tgccacaggg aggttctggt
    11341 ggattgttgg atcctggaac tccaatggac gctgatcttg ttgcttcctc tactgtcgtt
    11401 tgggagcaag acgctgaccc tttcgctgga accgctgacg atttcccagc ctttaacgaa
    11461 gaggaattgg cctggcttat ggagttgctt cctcaggcta ggggaggatc tggtggtctt
    11521 ttggatccag gaacccctat ggatgctgac cttgttgctt cttccaccgt tgtctgggag
    11581 caggatgccg accctttcgc tggtaccgca gacgacttcc cagcttttaa cgaggaagag
    11641 cttgcttggc tcatggaatt gttgccacag ggaggttctg gtggattgtt ggatcctgga
    11701 actccaatgg acgctgatct tgttgcttcc tctactgtcg tttgggagca agacgctgac
    11761 cctttcgctg gaaccgctga cgatttccca gcctttaacg aagaggaatt ggcctggctt
    11821 atggagttgc ttcctcaggc taggggtgga tctggaggtg gtggtagcgg aggagatgct
    11881 cttgatgatt tcgatcttga tatgcttgga tctgacgctc tcgacgactt tgacctcgac
    11941 atgttgggat ctgatgctct tgacgatttc gatttggaca tgttgggttc tgacgccttg
    12001 gacgatttcg acctcgacat gcttgctagg ggatctgacg cccttgatga tttcgacttg
    12061 gatatgcttg gatctgacgc ccttgatgat ttcgacttgg atatgcttgg atctgacgcc
    12121 cttgatgatt tcgacttgga tatgcttgga tctgacgccc ttgatgattt cgacttggat
    12181 atgctttgac tgcagatatg aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct
    12241 tgtgtgctta agtttgtgtt tttttcttgg cttgttgtgt tatgaatttg tggctttttc
    12301 taatattaaa tgaatgtaag atcacattat aatgaataaa caaatgtttc tataatccat
    12361 tgtgaatgtt ttgttggatc tcttctgcag catataacta ctgtatgtgc tatggtatgg
    12421 actatggaat atgattaaag ataaggagct caaactatca gtgtttgaca ggatatattg
    12481 gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg
    12541 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga
    12601 tcaaagtact ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt
    12661 gcagccgtct tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc
    12721 cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg
    12781 cgactagaac cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta
    12841 tgcccgcgtc agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc
    12901 cggctgcacc aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct
    12961 ggccaggatg cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg
    13021 cctggcccgc agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc
    13081 gggcctgcgt agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt
    13141 gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg
    13201 gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac
    13261 cccggcacag atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga
    13321 ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga
    13381 ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga
    13441 ggccgacgcc ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac
    13501 caggacggcc aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg
    13561 gccgggtacg tgttcgagcc gcccgcgcac ggctcaaccg tgcggctgca tgaaatcctg
    13621 gccggtttgt ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc
    13681 gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc
    13741 gctgcgtata tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta
    13801 tcgctgtact taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc
    13861 gcgccctgca actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg
    13921 cccgcgattg ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc
    13981 cgacgattga ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag
    14041 cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc
    14101 cggtgcagcc aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc
    14161 agcgcattga ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca
    14221 aaggcacgcg catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc
    14281 ttgagtcccg tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg
    14341 ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa
    14401 ttaaatcaaa actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac
    14461 gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc
    14521 agacacgcca gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct
    14581 gaagatgtac gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc
    14641 gcagctacca gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag
    14701 gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg
    14761 gaggaacggg cggttggcca ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca
    14821 ctggaacccc caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca
    14881 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
    14941 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
    15001 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
    15061 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
    15121 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
    15181 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
    15241 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
    15301 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
    15361 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
    15421 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
    15481 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
    15541 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
    15601 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
    15661 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
    15721 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
    15781 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
    15841 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
    15901 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
    15961 ggaaaaaggt cgaaaaggcc tctttcctgt ggatagcacg tacattggga acccaaagcc
    16021 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
    16081 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
    16141 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
    16201 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
    16261 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
    16321 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
    16381 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
    16441 aaacggtcac agcttgtctg taagcggatg ccgggagcag acaagcgcgt cagcgggtgt
    16501 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
    16561 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
    16621 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
    16681 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
    16741 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    16801 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    16861 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    16921 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gct
//
