LOCUS       sequence-417423-        9563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Dox-inducible Pax7 together with a GFP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9563)
  AUTHORS   Rao L, Qian Y, Khodabukus A, Ribar T, Bursac N
  TITLE     Engineering human pluripotent stem cells into a functional skeletal 
            muscle tissue.
  JOURNAL   Nat Commun. 2018 Jan 9;9(1):126. doi: 10.1038/s41467-017-02636-4.
  PUBMED    29317646
REFERENCE   2  (bases 1 to 9563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-01-9"; volume: "9(1):126. doi"; pages: " 
            10.1038/s41467-017-02636-4"
COMMENT     Sequence Label: pRRL-TetO-Pax7-IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..9563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..234
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             235..415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    462..587
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1080..1313
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1498..1542
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1840..1957
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2010..2028,2029..2051,2052..2070,2071..2093,2094..2112,
                     2113..2135,2136..2154,2155..2177,2178..2196,2197..2219,
                     2220..2238,2239..2261,2262..2280)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2313..2351
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2348..2372
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2463..2472
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2467..2476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4175..4637
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4231..4248)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4458..4477
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4651..4653,4654..4656,4657..5370)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4651..4653,4654..4656,4657..5367)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4696..4717)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4957..4976)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5304..5325
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5410..5998
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5463..5483)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5881..5892)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6089..6322
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6394..6515
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6431..6450)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6485..6504
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6555..6690
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6710..6729)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6711..6729)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6739..6756)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6739..6755)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6748..6770)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6897..7352
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6984..7003)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7194..7215
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7378..7482
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7483..7551,7552..8343)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7701..7720)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8514..9102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9003..9022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9256..9273
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9390..9411
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9426..9431,9432..9449,9450..9456)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9464..9480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9469..9491
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9488..9504
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9488..9504
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9523..9543
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9525..9543
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
      241 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      301 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      361 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
      421 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
      481 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
      541 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
      601 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
      661 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
      721 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
      781 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      841 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      901 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      961 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1021 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1081 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1141 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1201 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1261 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1321 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1381 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     1441 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     1501 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     1561 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     1621 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     1681 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1741 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     1801 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     1861 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     1921 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     1981 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2041 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2101 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2161 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2221 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2281 aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata
     2341 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2401 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccccgaattc gttaacggat
     2461 ccgccaccat ggcggccctt cccggcacgg taccgagaat gatgcggccg gctccggggc
     2521 agaactaccc ccgcacggga ttccctttgg aagtgtccac cccgcttggc caaggccggg
     2581 tcaatcagct gggaggggtc ttcatcaatg ggcgacccct gcctaaccac atccgccaca
     2641 agatagtgga gatggcccac catggcatcc ggccctgtgt catctcccga cagctgcgtg
     2701 tctcccacgg ctgcgtctcc aagattcttt gccgctacca ggagaccggg tccatccggc
     2761 ctggggccat cggcggcagc aagcccagac aggtggcgac tccggatgta gagaaaaaga
     2821 ttgaggagta caagagggaa aacccaggca tgttcagctg ggagatccgg gacaggctgc
     2881 tgaaggatgg gcactgtgac cgaagcactg tgccctcagt gagttcgatt agccgcgtgc
     2941 tcagaatcaa gttcgggaag aaagaggagg aggatgaagc ggacaagaag gaggacgacg
     3001 gcgaaaagaa ggccaaacac agcatcgacg gcatcctggg cgacaaaggg aaccggctgg
     3061 acgagggctc ggatgtggag tcggaacctg acctcccact gaagcgcaag cagcgacgca
     3121 gtcggaccac attcacggcc gagcagctgg aggagctgga gaaggccttt gagaggaccc
     3181 actacccaga catatacacc cgcgaggagc tggcgcagag gaccaagctg acagaggcgc
     3241 gtgtgcaggt ctggttcagt aaccgccgcg cccgttggcg taagcaggca ggagccaacc
     3301 agctggcggc gttcaaccac cttctgccag gaggcttccc gcccaccggc atgcccacgc
     3361 tgccccccta ccagctgccg gactccacct accccaccac caccatctcc caagatgggg
     3421 gcagcactgt gcaccggcct cagcccctgc caccgtccac catgcaccag ggcgggctgg
     3481 ctgcagcggc tgcagccgcc gacaccagct ctgcctacgg agcccgccac agcttctcca
     3541 gctactctga cagcttcatg aatccggcgg cgccctccaa ccacatgaac ccggtcagca
     3601 acggcctgtc tcctcaggtg atgagcatct tgggcaaccc cagtgcggtg cccccgcagc
     3661 cacaggctga cttctccatc tccccgctgc atggcggcct ggactcggcc acctccatct
     3721 cagccagctg cagccagcgg gccgactcca tcaagccagg agacagcctg cccacctccc
     3781 aggcctactg cccacccacc tacagcacca ccggctacag cgtggacccc gtggccggct
     3841 atcagtacgg ccagtacggc cagagtgagt gcctggtgcc ctgggcgtcc cccgtcccca
     3901 ttccttctcc cacccccagg gcctcctgct tgtttatgga gagctacaag gtggtgtcag
     3961 ggtggggaat gtccatttca cagatggaaa aattgaagtc cagccagatg gaacagttca
     4021 cctaactcga gttcgaaaac ggatctccgc ggccccgaat tccscccycy cccycccccc
     4081 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     4141 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     4201 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     4261 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     4321 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     4381 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     4441 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     4501 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     4561 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     4621 gaaaaacacg atgataagct tgccacaacc atggtgagca agggcgagga gctgttcacc
     4681 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     4741 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     4801 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     4861 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     4921 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     4981 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     5041 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     5101 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     5161 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     5221 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     5281 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     5341 actctcggca tggacgagct gtacaagtaa gggatcatcg ctagcaccgg tctgcagtct
     5401 agagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5461 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5521 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5581 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5641 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5701 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5761 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     5821 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5881 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     5941 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     6001 ggaattaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6061 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     6121 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6181 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6241 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6301 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     6361 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     6421 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6481 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     6541 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6601 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6661 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     6721 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     6781 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     6841 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     6901 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     6961 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7021 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7081 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7141 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7201 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     7261 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     7321 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     7381 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7441 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7501 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7561 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7621 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7681 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     7741 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7801 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7861 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     7921 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7981 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     8041 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8101 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8161 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8221 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8281 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8341 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8401 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8461 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8521 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8581 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8641 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     8701 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8761 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8821 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8881 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8941 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9001 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9061 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9121 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9181 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9241 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     9301 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     9361 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     9421 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     9481 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     9541 agggaacaaa agctggagct gca
//
