LOCUS       sequence-417424-       19583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The T-DNA backbone of Cas12j2 for DNA methylation-based gene 
            silencing in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19583)
  AUTHORS   Liu S, Sretenovic S, Fan T, Cheng Y, Li G, Qi A, Tang X, Xu Y, Guo 
            W, Zhong Z, He Y, Liang Y, Han Q, Zheng X, Gu X, Qi Y, Zhang Y
  TITLE     Hypercompact CRISPR-Cas12j2 (CasPhi) enables genome editing, gene 
            activation, and epigenome editing in plants.
  JOURNAL   Plant Commun. 2022 Sep 20:100453. doi: 10.1016/j.xplc.2022.100453.
  PUBMED    36127876
REFERENCE   2  (bases 1 to 19583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2022 Sep 20:100453. doi: 10.1016/j.xplc.2022.100453."
COMMENT     Sequence Label: pGEL639
FEATURES             Location/Qualifiers
     source          1..19583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1629..2423)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    2848..2872
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    2950..3124
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3165..4190)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             complement(3165..4190)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(4257..4933)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(4300..4320)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    5124..5145
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5160..5165,5166..5183,5184..5190)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5198..5214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5203..5225
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5222..5238
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5222..5238
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5285..7282
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             9176..9196
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11489..11536
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             11576..11761
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             11774..11794
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11807..11827
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11840..11860
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      11876..12124
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     ncRNA           13692..13728
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     ncRNA           14171..14238
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(14594..14611)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14594..14610)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14603..14625)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    14813..14837
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             16138..16767
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             17196..18269
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      18335..18529
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(18765..18787)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    18873..19013
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     18928..18947
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(19028..19045)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(19199..19583,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(19279..19298)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtgctgag agatcccctc ataatttccc caaagcgtaa ccatgtgtga ataaattttg
      301 agctagtagg gttgcagcca cgagtaagtc ttcccttgtt attgtgtagc cagaatgccg
      361 caaaacttcc atgcctaagc gaactgttga gagtacgttt cgatttctga ctgtgttagc
      421 ctggaagtgc ttgtcccaac cttgtttctg agcatgaacg cccgcaagcc aacatgttag
      481 ttgaagcatc agggcgatta gcagcatgat atcaaaacgc tctgagctgc tcgttcggct
      541 atggcgtagg cctagtccgt aggcaggact tttcaagtct cggaaggttt cttcaatctg
      601 cattcgcttc gaatagatat taacaagttg tttgggtgtt cgaatttcaa caggtaagtt
      661 agttgctaga atccatggct cctttgccga cgctgagtag attttaggtg acgggtggtg
      721 acaatgagtc cgtgtcgagc gctgattttt tcggccttta gagcgagatt tatacaatag
      781 aatttggcat gagattggat tgcttttagt cagcctctta tagcctaaag tctttgagtg
      841 actagatgac atatcatgta agttgctgat aggtttccag ttttccgctc ctaggtctgc
      901 atattgtact tttcctctta ctcgacttaa ccagtaccaa cccagcttct caacggattt
      961 ataccatggc actttaaagc cagcatcact gacaatgagc ggtgtggtgt tactcggtag
     1021 aatgctcgca aggtcggcta gaaattggtc atgagctttc tttgaacatt gctctgaaag
     1081 cgggaacgct ttctcataaa gagtaacaga acgaccgtgt agtgcgactg aagctcgcaa
     1141 taccataagc cgtttttgct cacggatatc agaccagtca acaagtacaa tgggcatcgt
     1201 attgcccgaa cagataaagc tagcatgcca acggtataca gcgagtcgct ctttgtggag
     1261 gtgacgatta cctaacaatc ggtcgattcg tttgatgtta tgttttgttc tcgctttggt
     1321 tggcaggtta cggccaagtt cggtaagagt gagagtttta cagtcaagta aggcgtggca
     1381 agccaacgtt aagctgttga gtcgttttaa gtgtaattcg gggcagaatt ggtaaagaga
     1441 gtcgtgtaaa atatcgagtt cgcacatttt gttgtctgat tattgatttt tggcgaaacc
     1501 atttgatcat atgacaagat gtgtatctac cttaacttaa tgattttgat aaaaatcatt
     1561 aggggattca tcagcgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat
     1621 tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc
     1681 ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg
     1741 atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga
     1801 tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg
     1861 tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc
     1921 ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta
     1981 ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc
     2041 gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca
     2101 gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc
     2161 agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct
     2221 tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg
     2281 ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata taccttagca
     2341 ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc
     2401 ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga
     2461 taccccaaga agctaattat aacaagacga actccaattc actgttcctt gcattctaaa
     2521 accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag
     2581 tatcgacgga gccgattttg aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt
     2641 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
     2701 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
     2761 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
     2821 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
     2881 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt
     2941 aattcggggg atctggattt tagtactgga ttttggtttt aggaattaga aattttattg
     3001 atagaagtat tttacaaata caaatacata ctaagggttt cttatatgct caacacatga
     3061 gcgaaaccct ataggaaccc taattccctt atctgggaac tactcacaca ttattatgga
     3121 gaaactcgag cttgtcgatc gacagatccc ggtcggcatc tactctattt ctttgccctc
     3181 ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc
     3241 cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg
     3301 acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag
     3361 ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagtcg tggcgatcct
     3421 gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac
     3481 ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc
     3541 cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc
     3601 gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga
     3661 gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg
     3721 ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt
     3781 ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc
     3841 tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca
     3901 ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc
     3961 acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag
     4021 aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg
     4081 aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggacacgct gtcgaacttt
     4141 tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat atctcattgc
     4201 cccccggatc tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag
     4261 cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag
     4321 tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa
     4381 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
     4441 ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat
     4501 ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa
     4561 tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg
     4621 tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg
     4681 ttcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
     4741 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc
     4801 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
     4861 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
     4921 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
     4981 gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct
     5041 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     5101 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     5161 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     5221 acaggaaaca gctatgacat gattacgaat tcgagctcgg tacccgggga tcggcgcgcc
     5281 ggagctgcag tgcagcgtga cccggtcgtg cccctctctt gagataaggt gagcattgca
     5341 tgtctaagtt ataaaaaatt accacatatt ttttttgtca cacttgtttg aagtgcagtt
     5401 tatctatctt tatacatata tttaaacttt actctacgaa taatataatc tatagtacta
     5461 caataatatc agtgttttag agaatcatat aaatgaacag ttagacatgg tctaaaggac
     5521 aattgagtat tttgacaaca ggactctaca gttttatctt tttagtgtgc atgtgttctc
     5581 cttttttttt gcaaatagct tcacctatat aatacttcat ccattttatt agtacatcca
     5641 tttagggttt agggttaatg gtttttatag actaattttt ttagtacatc tattttattc
     5701 tattttagcc tctaaattaa gaaaactaaa actctatttt agttttttta tttaataatt
     5761 tagatataaa atagaataaa ataaagtgac taaaaattaa acaaataccc tttaagaaat
     5821 taaaaaaact aaggaaacat ttttcttgtt tcgagtagat aatgccagcc tgttaaacgc
     5881 cgtcgacgag tctaacggac accaaccagc gaaccagcag cgtcgcgtcg ggccaagcga
     5941 agcagacggc acggcatctc tgtcgctgcc tctggacccc tctcgagagt tccgctccac
     6001 cgttggactt gctccgctgt cggcatccag aaattgcgtg gcggagcggc agacgtgagc
     6061 cggcacggca ggcggcctcc tcctcctctc acggcaccgg cagctacggg ggattccttt
     6121 cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagacac cccctccaca
     6181 ccctctttcc ccaacctcgt gttgttcgga gcgcacacac acacaaccag aactccccca
     6241 aatccacccg tcggcacctc cgcttcaagg tacgccgctc gtcctccccc ccccccctct
     6301 ctaccttctc aagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt
     6361 catgtttgtg ttagatccgt gtttgtgtta gatccgtgct actagcgttc gtacacggat
     6421 gcgacctgta cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat
     6481 cctgggatgg ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt
     6541 tgcatagggt ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc
     6601 gggtcatctt ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt
     6661 cgttcaagat cggagtagaa ttaattctgt ttcaaactac ctggtggatt tattaatttt
     6721 ggatctgtat gtgtgtgcca tacatattca tagttacgaa ttgaagatga tggatggaaa
     6781 tatcgatcta ggataggtat acatgttgat gcgggtttta ctgatgcata tacagagatg
     6841 ctttttgttc gcttggttgt gatgatgtgg tgtggttggg cggtcgttca ttcgttcaag
     6901 atcggagtag aatactgttt caaactacct ggtgtattta ttaattttgg aactgtatgt
     6961 gtgtgtcata catcttcata gttacgagtt taagatggat ggaaatatcg atctaggata
     7021 ggtatacatg ttgatgtggg ttttactgat gcatatacat gatggcatat gcagcatcta
     7081 ttcatatgct ctaaccttga gtacctatct attataataa acaagtatgt tttataatta
     7141 ttttgatctt gatatacttg gatgatggca tatgcagcag ctatatgtgg atttttttag
     7201 ccctgccttc atacgctatt tatttgcttg gtactgtttc ttttgtcgat gctcaccctg
     7261 ttgtttggtg ttacttctgc agcctgcagg aatgaaccat gatcaagaat ttgatcctcc
     7321 caaggtgtac ccgcccgttc cagccgagaa aaggaagccg atccgagttc ttagtttatt
     7381 cgacggcatc gccaccgggc tgctggtatt gaaggatctc ggcattcaag ttgacaggta
     7441 catagcatct gaggtctgtg aagattcaat aactgtcggc atggttcgtc atcagggcaa
     7501 gattatgtac gtgggagatg ttagaagtgt aacgcagaag catatccagg agtggggccc
     7561 ttttgatctg gtaatcggtg gttcaccttg caacgacttg tccattgtga acccggcgcg
     7621 gaagggcctc tatgagggga ccggccggct cttcttcgag ttctacagac tgctccacga
     7681 cgctcgtcca aaagagggtg acgaccgccc tttcttttgg ctctttgaga atgtcgtcgc
     7741 catgggggta agcgacaaac gcgacattag cagatttctt gaatccaacc ctgtgatgat
     7801 cgacgccaag gaggtgtcag cggcgcacag agcacggtac ttctggggta atttgcctgg
     7861 gatgaacagg cctcttgcat cgaccgtcaa tgataaactg gagctgcagg agtgccttga
     7921 gcatggaaga attgctaagt tctcgaaagt tcgcaccatc accacaagat ccaattccat
     7981 caagcagggg aaggaccagc atttcccggt ctttatgaat gaaaaggagg acatactatg
     8041 gtgcactgag atggagcggg tgtttggctt ccccgtgcac tacaccgatg tgtccaacat
     8101 gagccggctc gcccggcaga ggctgctggg acgatcatgg tccgtgccgg tgattaggca
     8161 cctttttgca ccattgaaag aatattttgc ttgtgtctcg tctggcaatt caaatgccaa
     8221 ctctagggga ccgtctttct catcaggcct ggtccccctc tctctgcgcg gcagccacat
     8281 ggggcccatg gaaatataca agacggtgtc ggcgtggaag aggcaaccag tgagggtctt
     8341 gagtctgttc cgcaacattg ataaggtcct gaaatctttg gggttcttgg aatctggatc
     8401 gggatcaggt ggtggcaccc tcaaatatgt ggaggatgtg acaaatgttg ttcgaagaga
     8461 tgttgagaag tggggaccat ttgacctcgt ctatggtagt acacagcccc ttgggtcatc
     8521 ttgtgatcgc tgcccagggt ggtacatgtt ccagttccac cgcatactac aatatgctct
     8581 gccgcgtcaa gagtctcaaa ggccattttt ctggatcttc atggataatc ttttattgac
     8641 tgaagatgat caggaaacaa ctactcgctt cctccaaaca gaagcagtca cactccaaga
     8701 tgtacgcggg agggactacc agaatgctat gagggtgtgg tcgaacatcc caggactgaa
     8761 atccaagcat gctccgctca ccccaaagga agaggagtac ctacaagcac aggtgcggtc
     8821 aaggagcaag ctcgacgcgc ccaaagttga tcttttagtg aagaactgcc tcctaccctt
     8881 aagagagtat ttcaagtatt tttctcagaa cagcctgccc ttgggtggcc cgtccagcgg
     8941 cgcccctcct ccatcaggtg ggagccctgc cggcagccct acttccacag aggagggaac
     9001 gtccgagagc gcgacgccgg agtcaggacc aggcacctcc acagaaccat ctgagggctc
     9061 ggcgccgggc agcccagctg gttcgccgac ttccactgaa gaaggtacga gtaccgaacc
     9121 ttcagaaggc tccgcccccg gcacgtcgac ggagccgagt gagggttcga tggctccgaa
     9181 gaagaagagg aaggttggca tccacggggt gccagctgct cctaaaccgg ctgtcgaaag
     9241 cgaattctct aaagtgttga aaaagcactt ccctggcgag agattcagat catcctacat
     9301 gaaaagaggg gggaagattc tggcggctca aggagaggaa gccgtcgtgg catatctcca
     9361 gggcaaatcc gaggaggaac caccgaactt tcaacctcca gcaaagtgcc atgtggtcac
     9421 gaagtcccgg gattttgcgg agtggccaat catgaaggca agtgaagcca tacagcgcta
     9481 tatctacgcc cttagcacta ctgaaagagc ggcgtgtaag cctggaaaat cctctgagtc
     9541 gcacgcggca tggtttgctg ctacaggggt gtctaatcat ggatatagcc atgttcaggg
     9601 tcttaacctg atctttgacc atacactggg acgctacgat ggggtgttga agaaggttca
     9661 actgaggaat gaaaaagcaa gagcgcgcct tgagagcatc aacgcgagcc gcgctgacga
     9721 aggactcccg gaaatcaagg cagaagaaga agaagttgca acgaatgaga cgggacactt
     9781 gctgcagcca cccggaataa acccttcctt ttatgtttat cagactatca gccctcaggc
     9841 ttatcgcccc cgcgatgaga tcgtccttcc gcctgagtac gctggctacg ttagggaccc
     9901 aaacgcgcct ataccgttgg gcgtcgttag gaatagatgc gatatacaga aaggctgccc
     9961 agggtatata cccgagtggc agcgcgaggc tgggactgcg atatcaccta aaaccggcaa
    10021 agccgttacg gtgccgggat tgagtcctaa aaaaaataag agaatgagga ggtattggcg
    10081 gtcagagaag gagaaggccc aagatgcact ccttgtcacc gtgaggatag gcacggattg
    10141 ggtcgtcatt gacgttagag ggcttttgag gaacgcacgg tggaggacta ttgcgccgaa
    10201 ggacattagt ctgaacgcgc ttctcgatct gttcactggc gacccagtta ttgatgtgag
    10261 gcggaacata gtcaccttta cttatactct ggacgcatgc ggaacatacg ctcgcaagtg
    10321 gaccctgaaa ggaaagcaga ctaaggccac actggataag ttgacagcca cgcagacagt
    10381 cgctttggtg gccatagacc ttgggcaaac caacccaatt agtgccggga tcagcagggt
    10441 tactcaggag aatggtgcac ttcagtgcga gcctctcgat cgctttacac tccccgatga
    10501 tcttcttaaa gacatcagtg cctacagaat cgcctgggac cggaacgagg aggaactccg
    10561 cgctcgcagt gttgaagcct tgccagaagc acagcaggct gaggttaggg cactggatgg
    10621 cgtttctaaa gagactgcca gaactcaatt gtgtgctgat tttgggcttg atcctaagag
    10681 actcccttgg gataaaatgt cgtcgaatac taccttcatc tccgaggccc tgcttagtaa
    10741 cagcgttagt cgcgaccaag tctttttcac acccgccccg aaaaaaggcg cgaaaaagaa
    10801 agctccggtg gaagttatga ggaaagaccg cacatgggca cgcgcttaca aaccgagact
    10861 gtcagttgaa gcgcagaaat tgaagaacga agcactttgg gccttgaagc ggacatctcc
    10921 agagtatctt aaactctcca ggagaaagga agaactctgt agaaggtcga ttaattacgt
    10981 tatagagaag acacgccgga ggactcagtg tcaaattgtt attccggtca tcgaagacct
    11041 caatgttcgc ttctttcatg gttctggaaa gcggttgccc ggatgggaca atttctttac
    11101 agctaaaaaa gaaaaccgct ggttcataca aggattgcac aaggccttta gtgatctccg
    11161 gacgcatcgc tccttttatg tcttcgaggt gaggccggaa agaacgtcca tcacatgccc
    11221 aaagtgtgga cattgtgaag tcgggaaccg ggacggtgag gcctttcaat gcctttcgtg
    11281 cgggaagacc tgtaatgcgg atcttgatgt ggctacgcat aatctcactc aggtggctct
    11341 cacaggcaaa acaatgccga agagagagga acctagggat gcacaaggga cggccccggc
    11401 cagaaaaacg aagaaggcta gcaagtccaa ggcgccgccg gcggagaggg aggatcagac
    11461 tccagcccag gaaccatccc aaacaagtaa gcggccagcg gcgacgaaga aggcggggca
    11521 ggcgaagaag aagaaggcag gatcgatgga tgccaagagc ctgacagcgt ggtcacgcac
    11581 cctcgtcaca ttcaaggacg tgttcgtcga tttcacccgg gaggagtgga agctcctgga
    11641 cacagcccag cagatcgtgt acaggaatgt catgctggag aactacaaga atctcgtgtc
    11701 cctgggctac cagctcacca agccggatgt cattctccgc ctggagaagg gcgaggagcc
    11761 atccggcggc agcccaaaga agaagcggaa ggtgtctgga ggttctccta agaaaaagag
    11821 aaaagtgtcc ggcggctccc cgaagaagaa gcgcaaggtg tgagcttctc gagtatatga
    11881 agatgaagat gaaatatttg gtgtgtcaaa taaaaagctt gtgtgcttaa gtttgtgttt
    11941 ttttcttggc ttgttgtgtt atgaatttgt ggctttttct aatattaaat gaatgtaaga
    12001 tcacattata atgaataaac aaatgtttct ataatccatt gtgaatgttt tgttggatct
    12061 cttctgcagc atataactac tgtatgtgct atggtatgga ctatggaata tgattaaaga
    12121 taagcgctga gctcggacgg cgcgcccaca tcagtctctg cacaaagtgc atcctgggct
    12181 gcttcaatta taaagcccca ttcaccacat ttgctagata gtcgaaaagc accatcaata
    12241 ttgagcttca ggtatttttg gttgtgttgt ggttggattg attctaatat ataccaaatc
    12301 aatataattc actaccaaaa tataccatag ccatcacaac tttattaatt ttggtagctt
    12361 aagatggtat atataataac caattaacaa ctgattctaa ttttactacg gcccagtatc
    12421 taccaataca aaacaacgag tatgttttct tccgtcgtaa tcgtacacag tacaaaaaaa
    12481 cctggccagc ctttcttggg ctggggctct ctttcgaaag gtcacaaaac gtacacggca
    12541 gtaacgccgc ttcgctgcgt gttaacggcc accaaccccg ccgtgagcaa acggcatcag
    12601 ctttccacct cctcgatatc tccgcggcgc cgtctggacc cgcccccttt ccgttccttt
    12661 ctttccttct cgcgtttgcg tggtggggac ggactcccca aaccgcctct ccctctcttt
    12721 atttgtctat attctcactg ggccccaccc accgcacccc tgggcccact cacgagtccc
    12781 cccctcccca cctataaata ccccaccccc tcctcgcctc ttcctccatc aatcgaatcc
    12841 ccaaaatcgc agagaaaaaa aaatctcccc tcgaagcgaa gcgtcgaatc gccttctcaa
    12901 ggtatgcgat tttctgatcc tctccgttcc tcgcgtttga tttgatttcc cggcctgttc
    12961 gtgattgtga gatgttgttg ttagtctccg ttttgcgatc tgtggtagat ttgaacaggg
    13021 ttagatgggg ttcgcgtggt atgctggatc tgtgattatg agcgatgctg ttcgtggtcc
    13081 aagtatttat tggttcggat ctagaagtag aactgtgcta gggttgtgat ttgttccgat
    13141 ctgttcaatc agtaggattt agtctctgtt tttctcgttg atccaagtag cagcttcagg
    13201 tatattttgc ttaggttgtt tttgattcag tccctctagt tgcatagatt ctactctgtt
    13261 catgtttaat ctaagggctg cgtcttgttg attagtgatt acatagcata gctttcagga
    13321 tattttactt gcttatgcct atcttatcaa ctgttgcacc tgtaaattct agcctatgtt
    13381 aattaacctg ccttatgtgc tctcgggata gtgctagtag ttattgaatc agtttgccga
    13441 tggaattcta gtagttcata gacctgcaga ttatttttgt gaactcgagc acggtgcgac
    13501 tctctatttt gttaggtcac tgttggtgtt gataggtaca ctgatgttat tgtggtttag
    13561 atcgtgtatc taacatattg gaataatttg attgactgat ttctgctgta cttgcttggt
    13621 attgttataa tttcatgttc atagttgctg accatgcttc ggtaattgtg tgtgcagcct
    13681 gcaggtcgtt gctgatgagt ccgtgaggac gaaacgagta agctcgtcca acgattgccc
    13741 ctcacgaggg gacggagacc gggcgtgtca ataatatcac tctgtacatc cacaaacaga
    13801 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt
    13861 ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg
    13921 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct
    13981 taccagaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca
    14041 actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca
    14101 tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg
    14161 cttggtctca ggccggcatg gtcccagcct cctcgctggc gccggctggg caacatgctt
    14221 cggcatggcg aatgggacac tcgagtccta gacttgtcca tcttctggat tggccaagtt
    14281 aattaatgta tgaaataaaa ggatgcacac atagtgacat gctaatcact ataatgtggg
    14341 catcaaagtt gtgtgttatg tgtaattact aattatctga ataagagaaa gagatcatcc
    14401 atatttctta tcctaaatga atgtcacgtg tctttataat tctttgatga accagatgca
    14461 ttttattaac caattccata tacatataaa tattaatcat atataattaa tatcaattgg
    14521 gttagcaaaa caaatctagt ctaggtgtgt tttgcgagct cccggggcgc gccgatatcg
    14581 agctcagtgt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
    14641 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
    14701 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgc tagagcagct
    14761 tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga
    14821 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa
    14881 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc
    14941 ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac
    15001 gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg
    15061 ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga
    15121 atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc
    15181 tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc
    15241 acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc
    15301 cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc
    15361 tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg
    15421 ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc
    15481 gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc
    15541 gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta
    15601 ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg
    15661 tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc
    15721 gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat
    15781 tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa
    15841 accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat
    15901 gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacggc tcaaccgtgc ggctgcatga
    15961 aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga
    16021 agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca
    16081 tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg
    16141 aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat
    16201 ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag
    16261 ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc
    16321 gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc
    16381 gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg
    16441 ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg
    16501 gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg
    16561 gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg
    16621 cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc
    16681 acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc
    16741 gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca
    16801 caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca
    16861 gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca
    16921 ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat
    16981 acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg
    17041 gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc
    17101 atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgcctg ccggccctgc
    17161 aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc
    17221 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg
    17281 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg
    17341 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga
    17401 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca
    17461 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac
    17521 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc
    17581 cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa
    17641 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc
    17701 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg
    17761 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga
    17821 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca
    17881 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga
    17941 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact
    18001 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca
    18061 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt
    18121 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg
    18181 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga
    18241 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc
    18301 tagcagggga aaaaggtcga aaaggcctct ttcctgtgga tagcacgtac attgggaacc
    18361 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga
    18421 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa
    18481 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc
    18541 agcgcacagc ccaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc
    18601 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc
    18661 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac
    18721 atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
    18781 ctcccggaaa cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
    18841 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
    18901 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
    18961 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggccctctt
    19021 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    19081 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    19141 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    19201 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    19261 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    19321 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    19381 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    19441 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    19501 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    19561 acaggattag cagagcgagg tat
//
