LOCUS       sequence-417425-       15471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The T-DNA backbone of Cas12j2 for genome editing in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15471)
  AUTHORS   Liu S, Sretenovic S, Fan T, Cheng Y, Li G, Qi A, Tang X, Xu Y, Guo 
            W, Zhong Z, He Y, Liang Y, Han Q, Zheng X, Gu X, Qi Y, Zhang Y
  TITLE     Hypercompact CRISPR-Cas12j2 (CasPhi) enables genome editing, gene 
            activation, and epigenome editing in plants.
  JOURNAL   Plant Commun. 2022 Sep 20:100453. doi: 10.1016/j.xplc.2022.100453.
  PUBMED    36127876
REFERENCE   2  (bases 1 to 15471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2022 Sep 20:100453. doi: 10.1016/j.xplc.2022.100453."
COMMENT     Sequence Label: pGEL635
FEATURES             Location/Qualifiers
     source          1..15471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(554..1348)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1773..1797
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1875..2049
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(3139..3815)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(3182..3202)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    4172..4196
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     ncRNA           complement(4216..4283)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(4726..4762)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     promoter        6322..8319
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             8334..8354
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10647..10694
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      10705..10953
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_feature    10974..10998
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12299..12928
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13357..14430
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14496..14690
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(14926..14948)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    15024..15164
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     15079..15098
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(15179..15196)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(15350..15471,1..467))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15430..15449)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
       61 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      121 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      181 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      241 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      301 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
      361 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      421 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
      481 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
      541 tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
      601 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
      661 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
      721 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
      781 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
      841 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
      901 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
      961 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
     1021 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
     1081 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
     1141 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
     1201 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
     1261 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
     1321 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
     1381 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
     1441 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
     1501 catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca
     1561 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
     1621 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct
     1681 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
     1741 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
     1801 ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc
     1861 gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt
     1921 tattgataga agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca
     1981 catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc acacattatt
     2041 atggagaaaa tagagcttgt cgatcgttaa ttaatcacct ccaagatccc ggacgagtgc
     2101 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
     2161 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
     2221 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
     2281 gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg
     2341 gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga
     2401 agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
     2461 ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga
     2521 ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga
     2581 cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
     2641 tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
     2701 cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg
     2761 gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac
     2821 ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa
     2881 tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
     2941 cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
     3001 attcttcgcc ctccgagagc tgcatcaggt cggacacgct gtcgaacttt tcgatcagaa
     3061 acttctcgac agacgtcgcg gtgagttcag gctttttcat cctaggagag atagatttgt
     3121 agagagagac tggtgatttc agcgtgtcct ctccaaatga aatgaacttc cttatataga
     3181 ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc
     3241 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
     3301 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
     3361 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
     3421 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
     3481 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
     3541 gagagtgtcg tgctccacca tgttcacatc aatccacttg ctttgaagac gtggttggaa
     3601 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
     3661 cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac
     3721 cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga
     3781 ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg
     3841 tatctttgat attcttggag tagacgagag tgtcgtgctc caccatggga ctagtagtaa
     3901 catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat
     3961 cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt
     4021 catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat
     4081 catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg
     4141 atcggggaaa ttcgaattaa ggaattatcg aaccactttg tacaagaaag ctgggtcgaa
     4201 ttccggccgg gtaccgtccc attcgccatg ccgaagcatg ttgcccagcc ggcgccagcg
     4261 aggaggctgg gaccatgccg gcctgagacc aagccagata acagtatgcg tatttgcgcg
     4321 ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag
     4381 gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa
     4441 ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt
     4501 cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg
     4561 tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa
     4621 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
     4681 tattgacacg cccggtctcc gtcccctcgt gaggggcaat cgttggacga gcttactcgt
     4741 ttcgtcctca cggactcatc agcaacgagg atccgatctg gatctgcaca cacaattacc
     4801 gaagcatggt cagcaactat gaacatgaaa ttataacaat accaagcaag tacagcagaa
     4861 atcagtcaat caaattattc caatatgtta gatacacgat ctaaaccaca ataacatcag
     4921 tgtacctatc aacaccaaca gtgacctaac aaaatagaga gacgcaccgt gctcgagttc
     4981 acaaaaataa tctgcaggtc tatgaactac tagaattcca tcggcaaact gattcaataa
     5041 ctactagcac tatcccgaga gcacataagg caggttaatt aacataggct agaatttaca
     5101 ggtgcaacag ttgataagat aggcataagc aagtaaaata tcctgaaagc tatgctatgt
     5161 aatcactaat caacaagacg cagcccttag attaaacatg aacagagtag aatctatgca
     5221 actagaggga ctgaatcaaa aacaacctaa gcaaaatata cctgaagctg ctacttggat
     5281 caacgagaaa aacagagact aaatcctact gattgaacag atcggaacaa atcacaaccc
     5341 tagcacagtt ctacttctag atccgaacca ataaatactt ggaccacgaa cagcatcgct
     5401 cataatcaca gatccagcat accacgcgaa ccccatctaa ccctgttcaa atctaccaca
     5461 gatcgcaaaa cggagactaa caacaacatc tcacaatcac gaacaggccg ggaaatcaaa
     5521 tcaaacgcga ggaacggaga ggatcagaaa atcgcatacc ttgagaaggc gattcgacgc
     5581 ttcgcttcga ggggagattt ttttttctct gcgattttgg ggattcgatt gatggaggaa
     5641 gaggcgagga gggggtgggg tatttatagg tggggagggg gggactcgtg agtgggccca
     5701 ggggtgcggt gggtggggcc cagtgagaat atagacaaat aaagagaggg agaggcggtt
     5761 tggggagtcc gtccccacca cgcaaacgcg agaaggaaag aaaggaacgg aaagggggcg
     5821 ggtccagacg gcgccgcgga gatatcgagg aggtggaaag ctgatgccgt ttgctcacgg
     5881 cggggttggt ggccgttaac acgcagcgaa gcggcgttac tgccgtgtac gttttgtgac
     5941 ctttcgaaag agagccccag cccaagaaag gctggccagg tttttttgta ctgtgtacga
     6001 ttacgacgga agaaaacata ctcgttgttt tgtattggta gatactgggc cgtagtaaaa
     6061 ttagaatcag ttgttaattg gttattatat ataccatctt aagctaccaa aattaataaa
     6121 gttgtgatgg ctatggtata ttttggtagt gaattatatt gatttggtat atattagaat
     6181 caatccaacc acaacacaac caaaaatacc tgaagctcaa tattgatggt gcttttcgac
     6241 tatctagcaa atgtggtgaa tggggcttta taattgaagc agcccaggat gcactttgtg
     6301 cagagactga tgtgggcgcg cctgcagtgc agcgtgaccc ggtcgtgccc ctctcttgag
     6361 ataaggtgag cattgcatgt ctaagttata aaaaattacc acatattttt tttgtcacac
     6421 ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact ctacgaataa
     6481 tataatctat agtactacaa taatatcagt gttttagaga atcatataaa tgaacagtta
     6541 gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt ttatcttttt
     6601 agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat acttcatcca
     6661 ttttattagt acatccattt agggtttagg gttaatggtt tttatagact aattttttta
     6721 gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact ctattttagt
     6781 ttttttattt aataatttag atataaaata gaataaaata aagtgactaa aaattaaaca
     6841 aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg agtagataat
     6901 gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa ccagcagcgt
     6961 cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct
     7021 cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg
     7081 gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag
     7141 ctacggggga ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa
     7201 tagacacccc ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca
     7261 caaccagaac tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc
     7321 ctcccccccc cccctctcta ccttctcaag atcggcgttc cggtccatgg ttagggcccg
     7381 gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctact
     7441 agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt
     7501 gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat
     7561 gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg
     7621 ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg
     7681 tggtctggtt gggcggtcgt tcaagatcgg agtagaatta attctgtttc aaactacctg
     7741 gtggatttat taattttgga tctgtatgtg tgtgccatac atattcatag ttacgaattg
     7801 aagatgatgg atggaaatat cgatctagga taggtataca tgttgatgcg ggttttactg
     7861 atgcatatac agagatgctt tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg
     7921 tcgttcattc gttcaagatc ggagtagaat actgtttcaa actacctggt gtatttatta
     7981 attttggaac tgtatgtgtg tgtcatacat cttcatagtt acgagtttaa gatggatgga
     8041 aatatcgatc taggataggt atacatgttg atgtgggttt tactgatgca tatacatgat
     8101 ggcatatgca gcatctattc atatgctcta accttgagta cctatctatt ataataaaca
     8161 agtatgtttt ataattattt tgatcttgat atacttggat gatggcatat gcagcagcta
     8221 tatgtggatt tttttagccc tgccttcata cgctatttat ttgcttggta ctgtttcttt
     8281 tgtcgatgct caccctgttg tttggtgtta cttctgcagc ctgcaggatg gctcctaaga
     8341 agaagcggaa ggttggtatt cacggggtgc ctgcggctcc taaaccggct gtcgaaagcg
     8401 aattctctaa agtgttgaaa aagcacttcc ctggcgagag attcagatca tcctacatga
     8461 aaagaggggg gaagattctg gcggctcaag gagaggaagc cgtcgtggca tatctccagg
     8521 gcaaatccga ggaggaacca ccgaactttc aacctccagc aaagtgccat gtggtcacga
     8581 agtcccggga ttttgcggag tggccaatca tgaaggcaag tgaagccata cagcgctata
     8641 tctacgccct tagcactact gaaagagcgg cgtgtaagcc tggaaaatcc tctgagtcgc
     8701 acgcggcatg gtttgctgct acaggggtgt ctaatcatgg atatagccat gttcagggtc
     8761 ttaacctgat ctttgaccat acactgggac gctacgatgg ggtgttgaag aaggttcaac
     8821 tgaggaatga aaaagcaaga gcgcgccttg agagcatcaa cgcgagccgc gctgacgaag
     8881 gactcccgga aatcaaggca gaagaagaag aagttgcaac gaatgagacg ggacacttgc
     8941 tgcagccacc cggaataaac ccttcctttt atgtttatca gactatcagc cctcaggctt
     9001 atcgcccccg cgatgagatc gtccttccgc ctgagtacgc tggctacgtt agggacccaa
     9061 acgcgcctat accgttgggc gtcgttagga atagatgcga tatacagaaa ggctgcccag
     9121 ggtatatacc cgagtggcag cgcgaggctg ggactgcgat atcacctaaa accggcaaag
     9181 ccgttacggt gccgggattg agtcctaaaa aaaataagag aatgaggagg tattggcggt
     9241 cagagaagga gaaggcccaa gatgcactcc ttgtcaccgt gaggataggc acggattggg
     9301 tcgtcattga cgttagaggg cttttgagga acgcacggtg gaggactatt gcgccgaagg
     9361 acattagtct gaacgcgctt ctcgatctgt tcactggcga cccagttatt gatgtgaggc
     9421 ggaacatagt cacctttact tatactctgg acgcatgcgg aacatacgct cgcaagtgga
     9481 ccctgaaagg aaagcagact aaggccacac tggataagtt gacagccacg cagacagtcg
     9541 ctttggtggc catagacctt gggcaaacca acccaattag tgccgggatc agcagggtta
     9601 ctcaggagaa tggtgcactt cagtgcgagc ctctcgatcg ctttacactc cccgatgatc
     9661 ttcttaaaga catcagtgcc tacagaatcg cctgggaccg gaacgaggag gaactccgcg
     9721 ctcgcagtgt tgaagccttg ccagaagcac agcaggctga ggttagggca ctggatggcg
     9781 tttctaaaga gactgccaga actcaattgt gtgctgattt tgggcttgat cctaagagac
     9841 tcccttggga taaaatgtcg tcgaatacta ccttcatctc cgaggccctg cttagtaaca
     9901 gcgttagtcg cgaccaagtc tttttcacac ccgccccgaa aaaaggcgcg aaaaagaaag
     9961 ctccggtgga agttatgagg aaagaccgca catgggcacg cgcttacaaa ccgagactgt
    10021 cagttgaagc gcagaaattg aagaacgaag cactttgggc cttgaagcgg acatctccag
    10081 agtatcttaa actctccagg agaaaggaag aactctgtag aaggtcgatt aattacgtta
    10141 tagagaagac acgccggagg actcagtgtc aaattgttat tccggtcatc gaagacctca
    10201 atgttcgctt ctttcatggt tctggaaagc ggttgcccgg atgggacaat ttctttacag
    10261 ctaaaaaaga aaaccgctgg ttcatacaag gattgcacaa ggcctttagt gatctccgga
    10321 cgcatcgctc cttttatgtc ttcgaggtga ggccggaaag aacgtccatc acatgcccaa
    10381 agtgtggaca ttgtgaagtc gggaaccggg acggtgaggc ctttcaatgc ctttcgtgcg
    10441 ggaagacctg taatgcggat cttgatgtgg ctacgcataa tctcactcag gtggctctca
    10501 caggcaaaac aatgccgaag agagaggaac ctagggatgc acaagggacg gccccggcca
    10561 gaaaaacgaa gaaggctagc aagtccaagg cgccgccggc ggagagggag gatcagactc
    10621 cagcccagga accatcccaa acaagtaagc gtcctgctgc caccaaaaag gccggacagg
    10681 ctaagaaaaa gaagtgatct agaatatgaa gatgaagatg aaatatttgg tgtgtcaaat
    10741 aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta tgaatttgtg
    10801 gctttttcta atattaaatg aatgtaagat cacattataa tgaataaaca aatgtttcta
    10861 taatccattg tgaatgtttt gttggatctc ttctgcagca tataactact gtatgtgcta
    10921 tggtatggac tatggaatat gattaaagat aaggagctca aactatcagt gtttgacagg
    10981 atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa
    11041 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
    11101 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
    11161 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
    11221 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
    11281 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
    11341 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
    11401 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
    11461 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
    11521 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
    11581 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
    11641 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
    11701 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
    11761 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
    11821 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
    11881 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
    11941 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
    12001 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
    12061 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg
    12121 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
    12181 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
    12241 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
    12301 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
    12361 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
    12421 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
    12481 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
    12541 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
    12601 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
    12661 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
    12721 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
    12781 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
    12841 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
    12901 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
    12961 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
    13021 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
    13081 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
    13141 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
    13201 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
    13261 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg
    13321 caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc
    13381 ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag
    13441 gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc
    13501 gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg
    13561 aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc
    13621 acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga
    13681 cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg
    13741 ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta
    13801 accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt
    13861 ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac
    13921 gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag
    13981 aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac
    14041 aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg
    14101 atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac
    14161 tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc
    14221 aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag
    14281 ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac
    14341 gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg
    14401 atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc
    14461 ctagcagggg aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta cattgggaac
    14521 ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg
    14581 aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa
    14641 aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc
    14701 cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc
    14761 cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg
    14821 ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca
    14881 catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
    14941 gctcccggaa acggtcacag cttgtctgta agcggatgcc gggagcagac aagcgcgtca
    15001 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
    15061 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    15121 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
    15181 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    15241 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    15301 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    15361 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    15421 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc t
//
