LOCUS       sequence-417437-       13273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of T7RNA polymerase and CRISPR endonunclease Fncas12a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13273)
  AUTHORS   Randazzo P, Bennis NX, Daran JM, Daran-Lapujade P
  TITLE     gEL DNA: A Cloning- and Polymerase Chain Reaction-Free Method for 
            CRISPR-Based Multiplexed Genome Editing.
  JOURNAL   CRISPR J. 2021 Apr 23. doi: 10.1089/crispr.2020.0028.
  PUBMED    33900846
REFERENCE   2  (bases 1 to 13273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "CRISPR J. 
            2021 Apr 23. doi: 10.1089/crispr.2020.0028."
COMMENT     Sequence Label: pUDE1084
FEATURES             Location/Qualifiers
     source          1..13273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      781..978
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      983..1965
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(join(2090..2881,2882..2950))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2713..2732
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2951..3055)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3158..3746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3647..3666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3818..4216
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             8121..8168
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8175..8255
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      8342..8588
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        8781..9447
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     9317..9340
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             9461..9481
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     gene            join(12677..13273,1..978)
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     CDS             join(13021..13273,1..776)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             join(13021..13273,1..776)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
ORIGIN
        1 gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc
       61 ctgaaaccga actgcccgct gttctgcaac cggtcgcgga ggccatggat gcgatcgctg
      121 cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat
      181 acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa
      241 ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt
      301 gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg
      361 tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg
      421 attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc
      481 agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg
      541 cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg
      601 atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg
      661 tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag
      721 tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaataaagta
      781 actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt
      841 gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc
      901 atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga
      961 atgctggtcg ctatactgga gtaacgaagc atctgtgctt cattttgtag aacaaaaatg
     1021 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa
     1081 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa
     1141 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     1201 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc
     1261 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt
     1321 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa
     1381 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac
     1441 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc
     1501 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg
     1561 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata
     1621 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct
     1681 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc
     1741 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc
     1801 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat
     1861 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcatcagag
     1921 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac
     1981 ttcccgagcg gccgcgatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2041 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2101 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2161 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2221 tgataccgcg ggacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2281 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2341 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2401 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2461 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2521 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2581 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2641 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2701 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2761 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2821 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2881 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2941 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3001 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3061 ttccccgaaa agtgccacct gtcatgacca aaatccctta acgtgagttt tcgttccact
     3121 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3181 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3241 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3301 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3361 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3421 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3481 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3541 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3601 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3661 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3721 cgtcaggggg gcggagccta tggaaaaacg ccagactata tgtgaaggca tggctatggc
     3781 acggcagaca ttccgccaga tcatcaatag gcacgctcat agcttcaaaa tgtttctact
     3841 ccttttttac tcttccagat tttctcggac tccgcgcatc gccgtaccac ttcaaaacac
     3901 ccaagcacag catactaaat ttcccctctt tcttcctcta gggtgtcgtt aattacccgt
     3961 actaaaggtt tggaaaagaa aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg
     4021 caataaaaat ttttatcacg tttctttttc ttgaaaattt tttttttgat ttttttctct
     4081 ttcgatgacc tcccattgat atttaagtta ataaacggtc ttcaatttct caagtttcag
     4141 tttcattttt cttgttctat tacaactttt tttacttctt gctcattaga aagaaagcat
     4201 agcaatctaa tctaagtttt atgagcatct accaggagtt cgtcaacaag tattcactga
     4261 gtaagacact gcggttcgag ctgatcccac agggcaagac actggagaac atcaaggccc
     4321 gaggcctgat tctggacgat gagaagcggg caaaagacta taagaaagcc aagcagatca
     4381 ttgataaata ccaccagttc tttatcgagg aaattctgag ctccgtgtgc atcagtgagg
     4441 atctgctgca gaattactca gacgtgtact tcaagctgaa gaagagcgac gatgacaacc
     4501 tgcagaagga cttcaagtcc gccaaggaca ccatcaagaa acagattagc gagtacatca
     4561 aggactccga aaagtttaaa aatctgttca accagaatct gatcgatgct aagaaaggcc
     4621 aggagtccga cctgatcctg tggctgaaac agtctaagga caatgggatt gaactgttca
     4681 aggctaactc cgatatcact gatattgacg aggcactgga aatcatcaag agcttcaagg
     4741 gatggaccac atactttaaa ggcttccacg agaaccgcaa gaacgtgtac tccagcaacg
     4801 acattcctac ctccatcatc taccgaatcg tcgatgacaa tctgccaaag ttcctggaga
     4861 acaaggccaa atatgaatct ctgaaggaca aagctcccga ggcaattaat tacgaacaga
     4921 tcaagaaaga tctggctgag gaactgacat tcgatatcga ctataagact agcgaggtga
     4981 accagagggt cttttccctg gacgaggtgt ttgaaatcgc caatttcaac aattacctga
     5041 accagtccgg cattactaaa ttcaatacca tcattggcgg gaagtttgtg aacggggaga
     5101 ataccaagcg caagggaatt aacgaataca tcaatctgta tagccagcag atcaacgaca
     5161 aaactctgaa gaaatacaag atgtctgtgc tgttcaaaca gatcctgagt gataccgagt
     5221 ccaagtcttt tgtcattgat aaactggaag atgactcaga cgtggtcact accatgcaga
     5281 gcttttatga gcagatcgcc gctttcaaga cagtggagga aaaatctatt aaggaaactc
     5341 tgagtctgct gttcgatgac ctgaaagccc agaagctgga cctgagtaag atctacttca
     5401 aaaacgataa gagtctgaca gacctgtcac agcaggtgtt tgatgactat tccgtgattg
     5461 ggaccgccgt cctggagtac attacacagc agatcgctcc aaagaacctg gataatccct
     5521 ctaagaaaga gcaggaactg atcgctaaga aaaccgagaa ggcaaaatat ctgagtctgg
     5581 aaacaattaa gctggcactg gaggagttca acaagcacag ggatattgac aaacagtgcc
     5641 gctttgagga aatcctggcc aacttcgcag ccatccccat gatttttgat gagatcgccc
     5701 agaacaaaga caatctggct cagatcagta ttaagtacca gaaccagggc aagaaagacc
     5761 tgctgcaggc ttcagcagaa gatgacgtga aagccatcaa ggatctgctg gaccagacca
     5821 acaatctgct gcacaagctg aaaatcttcc atattagtca gtcagaggat aaggctaata
     5881 tcctggataa agacgaacac ttctacctgg tgttcgagga atgttacttc gagctggcaa
     5941 acattgtccc cctgtataac aagattagga actacatcac acagaagcct tactctgacg
     6001 agaagtttaa actgaacttc gaaaatagta ccctggccaa cgggtgggat aagaacaagg
     6061 agcctgacaa cacagctatc ctgttcatca aggatgacaa gtactatctg ggagtgatga
     6121 ataagaaaaa caataagatc ttcgatgaca aagccattaa ggagaacaaa ggggaaggat
     6181 acaagaaaat cgtgtataag ctgctgcccg gcgcaaataa gatgctgcct aaggtgttct
     6241 tcagcgccaa gagtatcaaa ttctacaacc catccgagga catcctgcgg attagaaatc
     6301 actcaacaca tactaagaac gggagccccc agaagggata tgagaaattt gagttcaaca
     6361 tcgaggattg caggaagttt attgacttct acaagcagag catctccaaa caccctgaat
     6421 ggaaggattt tggcttccgg ttttccgaca cacagagata taactctatc gacgagttct
     6481 accgcgaggt ggaaaatcag gggtataagc tgacttttga gaacatttct gaaagttaca
     6541 tcgacagcgt ggtcaatcag ggaaagctgt acctgttcca gatctataac aaagattttt
     6601 cagcatacag caagggcaga ccaaacctgc atacactgta ctggaaggcc ctgttcgatg
     6661 agaggaatct gcaggacgtg gtctataaac tgaacggaga ggccgaactg ttttaccgga
     6721 agcagtctat tcctaagaaa atcactcacc cagctaagga ggccatcgct aacaagaaca
     6781 aggacaatcc taagaaagag agcgtgttcg aatacgatct gattaaggac aagcggttca
     6841 ccgaagataa gttctttttc cattgtccaa tcaccattaa cttcaagtca agcggcgcta
     6901 acaagttcaa cgacgagatc aatctgctgc tgaaggaaaa agcaaacgat gtgcacatcc
     6961 tgagcattga ccgaggagag cggcatctgg cctactatac cctggtggat ggcaaaggga
     7021 atatcattaa gcaggataca ttcaacatca ttggcaatga ccggatgaaa accaactacc
     7081 acgataaact ggctgcaatc gagaaggata gagactcagc taggaaggac tggaagaaaa
     7141 tcaacaacat taaggagatg aaggaaggct atctgagcca ggtggtccat gagattgcaa
     7201 agctggtcat cgaatacaat gccattgtgg tgttcgagga tctgaacttc ggctttaaga
     7261 gggggcgctt taaggtggaa aaacaggtct atcagaagct ggagaaaatg ctgatcgaaa
     7321 agctgaatta cctggtgttt aaagataacg agttcgacaa gaccggaggc gtcctgagag
     7381 cctaccagct gacagctccc tttgaaactt tcaagaaaat gggaaaacag acaggcatca
     7441 tctactatgt gccagccgga ttcacttcca agatctgccc cgtgaccggc tttgtcaacc
     7501 agctgtaccc taaatatgag tcagtgagca agtcccagga atttttcagc aagttcgata
     7561 agatctgtta taatctggac aaggggtact tcgagttttc cttcgattac aagaacttcg
     7621 gcgacaaggc cgctaagggg aaatggacca ttgcctcctt cggatctcgc ctgatcaact
     7681 ttcgaaattc cgataaaaac cacaattggg acactaggga ggtgtaccca accaaggagc
     7741 tggaaaagct gctgaaagac tactctatcg agtatggaca tggcgaatgc atcaaggcag
     7801 ccatctgtgg cgagagtgat aagaaatttt tcgccaagct gacctcagtg ctgaatacaa
     7861 tcctgcagat gcggaactca aagaccggga cagaactgga ctatctgatt agccccgtgg
     7921 ctgatgtcaa cggaaacttc ttcgacagca gacaggcacc caaaaatatg cctcaggatg
     7981 cagacgccaa cggggcctac cacatcgggc tgaagggact gatgctgctg ggccggatca
     8041 agaacaatca ggaggggaag aagctgaacc tggtcattaa gaacgaggaa tacttcgagt
     8101 ttgtccagaa tagaaataac aaaaggccgg cggccacgaa aaaggccggc caggcaaaaa
     8161 agaaaaaggg atcctaccca tacgatgttc cagattacgc ttatccctac gacgtgcctg
     8221 attatgcata cccatatgat gtccccgact atgcctaaat tagttatgtc acgcttacat
     8281 tcacgccctc cccccacatc cgctctaacc gttatgcatr cccatatgat gtccccgact
     8341 atgcctaaat tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc
     8401 gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg
     8461 ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg
     8521 tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct
     8581 ttaatttgat agcttccggc acatggtgag atgcactcta accgtcaggc gacgtataga
     8641 ttcgtatgta ctagcgttga atgttagcgt caacaacaag aagtttaatg acgcggaggc
     8701 caaggcaaaa agattccttg attacgtaag ggagttagaa tcattttgaa taaaaaacac
     8761 gctttttcag ttcgagttta tcattatcaa tactgccatt tcaaagaata cgtaaataat
     8821 taatagtagt gattttccta actttattta gtcaaaaaat tagcctttta attctgctgt
     8881 aacccgtaca tgcccaaaat agggggcggg ttacacagaa tatataacat cgtaggtgtc
     8941 tgggtgaaca gtttattcct ggcatccact aaatataatg gagcccgctt tttaagctgg
     9001 catccagaaa aaaaaagaat cccagcacca aaatattgtt ttcttcacca accatcagtt
     9061 cataggtcca ttctcttagc gcaactacag agaacagggg cacaaacagg caaaaaacgg
     9121 gcacaacctc aatggagtga tgcaacctgc ctggagtaaa tgatgacaca aggcaattga
     9181 cccacgcatg tatctatctc attttcttac accttctatt accttctgct ctctctgatt
     9241 tggaaaaagc tgaaaaaaaa ggttgaaacc agttccctga aattattccc ctacttgact
     9301 aataagtata taaagacggt aggtattgat tgtaattctg taaatctatt tcttaaactt
     9361 cttaaattct acttttatag ttagtctttt ttttagtttt aaaacaccaa gaacttagtt
     9421 tcgaataaac acacataaac aaacaaatat gctcgagcct ccaaaaaaga agagaaaggt
     9481 caacacgatt aacatcgcta agaacgactt ctctgacatc gaactggctg ctatcccgtt
     9541 caacactctg gctgaccatt acggtgagcg tttagctcgc gaacagttgg cccttgagca
     9601 tgagtcttac gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc aacttaaagc
     9661 tggtgaggtt gcggataacg ctgccgccaa gcctctcatc actaccctac tccctaagat
     9721 gattgcacgc atcaacgact ggtttgagga agtgaaagct aagcgcggca agcgcccgac
     9781 agccttccag ttcctgcaag aaatcaagcc ggaagccgta gcgtacatca ccattaagac
     9841 cactctggct tgcctaacca gtgctgacaa tacaaccgtt caggctgtag caagcgcaat
     9901 cggtcgggcc attgaggacg aggctcgctt cggtcgtatc cgtgaccttg aagctaagca
     9961 cttcaagaaa aacgttgagg aacaactcaa caagcgcgta gggcacgtct acaagaaagc
    10021 atttatgcaa gttgtcgagg ctgacatgct ctctaagggt ctactcggtg gcgaggcgtg
    10081 gtcttcgtgg cataaggaag actctattca tgtaggagta cgctgcatcg agatgctcat
    10141 tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct ggcgtagtag gtcaagactc
    10201 tgagactatc gaactcgcac ctgaatacgc tgaggctatc gcaacacgtg caggtgcgct
    10261 ggctggcatc tctctgatgt tccaaccttg cgtagttcct cctaagccgt ggactggcat
    10321 tactggtggt ggctattggg ctaacggtcg tcgtcctctg gcgctggtgc gtactcacag
    10381 taagaaagca ctgatgcgct acgaagacgt ttacatgcct gaggtgtaca aagcgattaa
    10441 cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc ctagcggtcg ccaacgtaat
    10501 caccaagtgg aagcattgtc cggtcgagga catccctgcg attgagcgtg aagaactccc
    10561 gatgaaaccg gaagacatcg acatgaatcc tgaggctctc accgcgtgga aacgtgctgc
    10621 cgctgctgtg taccgcaagg acaaggctcg caagtctcgc cgtatcagcc ttgagttcat
    10681 gcttgagcaa gccaataagt ttgctaacca taaggccatc tggttccctt acaacatgga
    10741 ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg caaggtaacg atatgaccaa
    10801 aggactgctt acgctggcga aaggtaaacc aatcggtaag gaaggttact actggctgaa
    10861 aatccacggt gcaaactgtg cgggtgtcga taaggttccg ttccctgagc gcatcaagtt
    10921 cattgaggaa aaccacgaga acatcatggc ttgcgctaag tctccactgg agaacacttg
    10981 gtgggctgag caagattctc cgttctgctt ccttgcgttc tgctttgagt acgctggggt
    11041 acagcaccac ggcctgagct ataactgctc ccttccgctg gcgtttgacg ggtcttgctc
    11101 tggcatccag cacttctccg cgatgctccg agatgaggta ggtggtcgcg cggttaactt
    11161 gcttcctagt gaaaccgttc aggacatcta cgggattgtt gctaagaaag tcaacgagat
    11221 tctacaagca gacgcaatca atgggaccga taacgaagta gttaccgtga ccgatgagaa
    11281 cactggtgaa atctctgaga aagtcaagct gggcactaag gcactggctg gtcaatggct
    11341 ggcttacggt gttactcgca gtgtgactaa gcgttcagtc atgacgctgg cttacgggtc
    11401 caaagagttc ggcttccgtc aacaagtgct ggaagatacc attcagccag ctattgattc
    11461 cggcaagggt ctgatgttca ctcagccgaa tcaggctgct ggatacatgg ctaagctgat
    11521 ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa gcaatgaact ggcttaagtc
    11581 tgctgctaag ctgctggctg ctgaggtcaa agataagaag actggagaga ttcttcgcaa
    11641 gcgttgcgct gtgcattggg taactcctga tggtttccct gtgtggcagg aatacaagaa
    11701 gcctattcag acgcgcttga acctgatgtt cctcggtcag ttccgcttac agcctaccat
    11761 taacaccaac aaagatagcg agattgatgc acacaaacag gagtctggta tcgctcctaa
    11821 ctttgtacac agccaagacg gtagccacct tcgtaagact gtagtgtggg cacacgagaa
    11881 gtacggaatc gaatcttttg cactgattca cgactccttc ggtaccattc cggctgacgc
    11941 tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac acatatgagt cttgtgatgt
    12001 actggctgat ttctacgacc agttcgctga ccagttgcac gagtctcaat tggacaaaat
    12061 gccagcactt ccggctaaag gtaacttgaa cctccgtgac atcttagagt cggacttcgc
    12121 gttcgcgtaa atccggagat tgataagact tttctagttg catatctttt atatttaaat
    12181 cttatctatt agttaatttt ttgtaattta tccttatata tagtctggtt attctaaaat
    12241 atcatttcag tatctaaaaa ttcccctctt ttttcagtta tatcttaaca ggcgacagtc
    12301 caaatgttga tttatcccag tccgattcat cagggttgtg aagcattttg tcaatggtcg
    12361 aaatcacatc agtaatagtg cctcttactt gcctcataga atttctttct cttaacgtca
    12421 ccgtttggtc ttttatagtt tcgaaatcta tggtgatacc gctgccaatg agacgacggg
    12481 gtcatcacgg ctcatcatgc gccaaacaaa tgtgtgcaat acacgctcgg atgactgcat
    12541 gatgaccgca ctgactgggg acagcagatc cacctaagcc tgtgagagaa gcagacaccc
    12601 gacagatcaa ggcagttaac tagtgcactg cagtacagtt tagcttgcct cgtccccgcc
    12661 gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg
    12721 cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata
    12781 atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc
    12841 gctccagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc
    12901 cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat
    12961 acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaaaa
    13021 atgggtaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
    13081 gacagcgtgt ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
    13141 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
    13201 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
    13261 attggggaat tta
//
