LOCUS       sequence-417451-        8514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 empty backbone for strain-specific barcode DNA tag through 
            Tn7 bacterial insertion, Kanamycin selectable marker and AmCyan1 
            fluorescent marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Ordon J, Thouin J, Nakano RT, Ma KW, Zhang P, Huettel B, 
            Garrido-Oter R, Schulze-Lefert P
  TITLE     Chromosomal barcodes for simultaneous tracking of near-isogenic 
            bacterial strains in plant microbiota.
  JOURNAL   Nat Microbiol. 2024 Apr;9(4):1117-1129. doi: 
            10.1038/s41564-024-01619-8. Epub 2024 Mar 19.
  PUBMED    38503974
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-024-01619-8"; journalName: "Nat Microbiol"; date: 
            "2024-04"; volume: "9"; issue: "4"; pages: "1117-1129"
COMMENT     Sequence Label: pBCC098
FEATURES             Location/Qualifiers
     source          1..8514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      308..402
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      504..590
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    complement(623..670)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(681..933)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(953..1504)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1546..1725)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    complement(1803..1850)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        join(1878..1883,1884..1899,1900..1906)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1914..1930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      2019..2028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2025..2717
                     /codon_start=1
                     /product="Anemonia majano cyan fluorescent protein"
                     /label=AmCyan
                     /translation="MALSNKFIGDDMKMTYHMDGCVNGHYFTVKGEGSGKPYEGTQTST
                     FKVTMANGGPLAFSFDILSTVFMYGNRCFTAYPTSMPDYFKQAFPDGMSYERTFTYEDG
                     GVATASWEISLKGNCFEHKSTFHGVNFPADGPVMAKMTTGWDPSFEKMTVCDGILKGDV
                     TAFLMLQGGGNYRCQFHTSYKTKKPVTMPPNHAVEHRIARTDLDKGGNSVQLTEHAVAH
                     ITSVVPFA"
     terminator      2731..2825
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        join(2974..2979,2980..2997,2998..3004)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3058..3717
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3124..3143)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4059..4364
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4243..4262
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     mobile_element  4518..4683
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        4726..5171
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(5172..5258,5259..6593)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     terminator      6676..6770
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(6891..6910)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6902..7717
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            7884..7992
                     /note="incP origin of transfer"
     rep_origin      8003..8391
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     terminator      complement(8422..8508)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttaattaaag cggataacaa tttcacacag gaggccgcct aggccgcggc cgcgcgaatt
       61 tgccgaattc ggatccggag ggagtgtggg cggacaataa agtcttaaac tgaacaaaat
      121 agatctaaac tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag
      181 tccagttatg ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat
      241 tttctgaagt gcaaattgcc cgtcgtatta aagaggggcg tggcgctgca agaattcaag
      301 cttggaggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      361 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gactgcgatg
      421 agtggcaggg cggggcgtaa tttcttaagg cagttattgg tgcccttaaa cgcctggggt
      481 aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      541 ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgcccgccg
      601 ctgcaagaat tcaagcttgg aggaagttcc tatactttct agagaatagg aacttcggaa
      661 taggaacttc aatgccggtc gatctagtaa catagatgac accgcgcgcg ataatttatc
      721 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
      781 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
      841 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
      901 taagaaactt tattgccaaa tgtttgaacg atctgcttga ctctaggggt catcagattt
      961 cggtgacggg caggaccgga cggggcggca ccggcaggct gaagtccagc tgccagaaac
     1021 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
     1081 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
     1141 cgctcttgaa gccctgtgcc tccagggact tcagcaggtg ggtgtagagc gtggagccca
     1201 gtcccgtccg ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg
     1261 cgttgcgtgc cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
     1321 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
     1381 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
     1441 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
     1501 tcatggtaga tcccctcgat cgagttgaga gtgaatatga gactctaatt ggataccgag
     1561 gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg
     1621 accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa
     1681 actccagaaa cccgcggctc agtggctcct tcaacgttgc ggttctgtca gttccaaacg
     1741 taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctcagc
     1801 ttgaagttcc tatactttct agagaatagg aacttcggaa taggtacttc cgctactaga
     1861 attcgagcgg ggaattcttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag
     1921 cggataacaa ttttcacaca ggaaacagct aaatgaccat gattacgcca agcttgcatg
     1981 cctgcaggtc gactctagag gatccacggg taccggtcgc caccatggct ctttcaaaca
     2041 agtttatcgg agatgacatg aaaatgacct accatatgga tggctgtgtc aatgggcatt
     2101 actttaccgt caaaggtgaa ggcagcggga agccatacga agggacgcag acctcgactt
     2161 ttaaagtcac catggccaac ggtgggcccc ttgcattctc ctttgacata ctatctacag
     2221 tgttcatgta tggaaatcga tgctttactg cgtatcctac cagtatgccc gactatttca
     2281 aacaagcatt tcctgacgga atgtcatatg aaaggacttt tacctatgaa gatggaggag
     2341 ttgctacagc cagttgggaa ataagcctta aaggcaactg ctttgagcac aaatccacgt
     2401 ttcatggagt gaactttcct gctgatggac ctgtgatggc gaagatgaca actggttggg
     2461 acccatcttt tgagaaaatg actgtctgcg atggaatatt gaagggtgat gtcaccgcgt
     2521 tcctcatgct gcaaggaggt ggcaattaca gatgccaatt ccacacttct tacaagacaa
     2581 aaaaaccggt gacgatgcca ccaaaccatg cggtggaaca tcgcattgcg aggaccgacc
     2641 ttgacaaagg tggcaacagt gttcagctga cggagcacgc tgttgcacat ataacctctg
     2701 ttgtcccttt cgcttaatta gctgagcttg gactcctgtt gatagatcca gtaatgacct
     2761 cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg
     2821 agaatctccc gctttacgaa ttcccatggg gagcatgcgg agttacgtca tccccacctt
     2881 ccgctgcgtt cttcatcgat gcccgcttag tgatatgctt aaaactccaa gcacagcctt
     2941 ctccccgggg cggccgcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     3001 aatgtgtgga ttttgagtta ggatccgtcg agattttcag gagctaagga agctaaaatg
     3061 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
     3121 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
     3181 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
     3241 attcttgccc gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag
     3301 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
     3361 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
     3421 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
     3481 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
     3541 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
     3601 aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc
     3661 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg
     3721 cgtggatccg gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc
     3781 ggtataagaa tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga
     3841 agcagcgtat tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga
     3901 tgtcaatatc tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc
     3961 cgaacgctgg aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat
     4021 gaacggctct tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct
     4081 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc
     4141 ccggacgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc
     4201 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata
     4261 tggccagtgt gccggtatcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa
     4321 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta
     4381 tacacagccc ccggggatgc tggttgtcat tgcccctcct tcctctaagt gataaggtta
     4441 cttcctctaa atgaccaagc agggtatcta atcctgttaa cgctcgctca gagaattcgc
     4501 atgcggagtc gagggagaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt
     4561 taattttcgt attagcttac gacgctacac ccagttccca tctattttgt cactcttccc
     4621 taaataatcc ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc
     4681 acacgctgag cgaattccac gcttgtggaa ttcctcgagg gagttcacat atacctgccg
     4741 ttcactatta tttagtgaaa tgagatatta tgatattttc tgaattgtga ttaaaaaggc
     4801 aactttatgc ccatgcaaca gaaactataa aaaatacaga gaatgaaaag aaacagatag
     4861 attttttagt tctttaggcc cgtagtctgc aaatcctttt atgattttct atcaaacaaa
     4921 agaggaaaat agaccagttg caatccaaac gagagtctaa tagaatgagg tcgaaaagta
     4981 aatcgcgcgg gtttgttact gataaagcag gcaagaccta aaatgtgtaa agggcaaagt
     5041 gtatactttg gcgtcacccc ttacatattt taggtctttt tttattgtgc gtaactaact
     5101 tgccatcttc aaacaggagg gctggaagaa gcagaccgct aacacagtac ataaaaaagg
     5161 agacatgaac gatgaacatc aaaaagtttg caaaacaagc aacagtatta acctttacta
     5221 ccgcactgct ggcaggaggc gcaactcaag cgtttgcgaa agaaacgaac caaaagccat
     5281 ataaggaaac atacggcatt tcccatatta cacgccatga tatgctgcaa atccctgaac
     5341 agcaaaaaaa tgaaaaatat caagttcctg aattcgattc gtccacaatt aaaaatatct
     5401 cttctgcaaa aggcctggac gtttgggaca gctggccatt acaaaacgct gacggcactg
     5461 tcgcaaacta tcacggctac cacatcgtct ttgcattagc cggagatcct aaaaatgcgg
     5521 atgacacatc gatttacatg ttctatcaaa aagtcggcga aacttctatt gacagctgga
     5581 aaaacgctgg ccgcgtcttt aaagacagcg acaaattcga tgcaaatgat tctatcctaa
     5641 aagaccaaac acaagaatgg tcaggttcag ccacatttac atctgacgga aaaatccgtt
     5701 tattctacac tgatttctcc ggtaaacatt acggcaaaca aacactgaca actgcacaag
     5761 ttaacgtatc agcatcagac agctctttga acatcaacgg tgtagaggat tataaatcaa
     5821 tctttgacgg tgacggaaaa acgtatcaaa atgtacagca gttcatcgat gaaggcaact
     5881 acagctcagg cgacaaccat acgctgagag atcctcacta cgtagaagat aaaggccaca
     5941 aatacttagt atttgaagca aacactggaa ctgaagatgg ctaccaaggc gaagaatctt
     6001 tatttaacaa agcatactat ggcaaaagca catcattctt ccgtcaagaa agtcaaaaac
     6061 ttctgcaaag cgataaaaaa cgcacggctg agttagcaaa cggcgctctc ggtatgattg
     6121 agctaaacga tgattacaca ctgaaaaaag tgatgaaacc gctgattgca tctaacacag
     6181 taacagatga aattgaacgc gcgaacgtct ttaaaatgaa cggcaaatgg tacctgttca
     6241 ctgactcccg cggatcaaaa atgacgattg acggcattac gtctaacgat atttacatgc
     6301 ttggttatgt ttctaattct ttaactggcc catacaagcc gctgaacaaa actggccttg
     6361 tgttaaaaat ggatcttgat cctaacgatg taacctttac ttactcacac ttcgctgtac
     6421 ctcaagcgaa aggaaacaat gtcgtgatta caagctatat gacaaacaga ggattctacg
     6481 cagacaaaca atcaacgttt gcgccaagct tcctgctgaa catcaaaggc aagaaaacat
     6541 ctgttgtcaa agacagcatc cttgaacaag gacaattaac agttaacaaa taaattcgct
     6601 gagcgaggat gcacatgtga ccgagggaag cttgcggccg cgtcgtgact gggaaaaccc
     6661 tggcgactag tcttggactc ctgttgatag atccagtaat gacctcagaa ctccatctgg
     6721 atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat ccaggggtcc
     6781 ccaataatta cgatttaaat ttgtgtctca aaatctctga tgttacattg cacaagataa
     6841 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     6901 tatgagccat attcagcgtg aaacgagctg tagccgtccg cgtctgaaca gcaacatgga
     6961 tgcggatctg tatggctata aatgggcgcg tgataacgtg ggtcagagcg gcgcgaccat
     7021 ttatcgtctg tatggcaaac cggatgcgcc ggaactgttt ctgaaacatg gcaaaggcag
     7081 cgtggcgaac gatgtgaccg atgaaatggt gcgtctgaac tggctgaccg aatttatgcc
     7141 gctgccgacc attaaacatt ttattcgcac cccggatgat gcgtggctgc tgaccaccgc
     7201 gattccgggc aaaaccgcgt ttcaggtgct ggaagaatat ccggatagcg gcgaaaacat
     7261 tgtggatgcg ctggccgtgt ttctgcgtcg tctgcatagc attccggtgt gcaactgccc
     7321 gtttaacagc gatcgtgtgt ttcgtctggc ccaggcgcag agccgtatga acaacggcct
     7381 ggtggatgcg agcgattttg atgatgaacg taacggctgg ccggtggaac aggtgtggaa
     7441 agaaatgcat aaactgctgc cgtttagccc ggatagcgtg gtgacccacg gcgattttag
     7501 cctggataac ctgattttcg atgaaggcaa actgattggc tgcattgatg tgggccgtgt
     7561 gggcattgcg gatcgttatc aggatctggc cattctgtgg aactgcctgg gcgaatttag
     7621 cccgagcctg caaaaacgtc tgtttcagaa atatggcatt gataatccgg atatgaacaa
     7681 actgcaattt catctgatgc tggatgaatt tttctaataa ttaattggac cgcggtccgc
     7741 gcgttgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta
     7801 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct
     7861 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg
     7921 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg
     7981 cgaggctggc cgtaggccgg ccgatctgaa gatcagcagt tcaacctgtt gatagtacgt
     8041 actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag ctctcatgtt
     8101 taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt actaagctct
     8161 catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaatataaa tcagcaactt
     8221 aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag gcttttaaag
     8281 cctttaaggt ttaacggttg tggacaacaa gccagggatg taacgcactg agaagccctt
     8341 agagcctctc aaagcaattt tgagtgacac aggaacactt aacggctgac atggggcgcg
     8401 cccagctgtc tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     8461 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcct
//
