LOCUS       sequence-417461-       11417 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 NSP3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Li J, Gui Q, Liang FX, Sall J, Zhang Q, Duan Y, Dhabaria A, Askenazi
            M, Ueberheide B, Stapleford KA, Pagano M
  TITLE     The REEP5/TRAM1 complex binds SARS-CoV-2 NSP3 and promotes virus 
            replication.
  JOURNAL   J Virol. 2023 Sep 28:e0050723. doi: 10.1128/jvi.00507-23.
  PUBMED    37768083
REFERENCE   2  (bases 1 to 11417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2023 Sep 28:e0050723. doi: 10.1128/jvi.00507-23."
COMMENT     Sequence Label: NSP3-FR-2Strep
FEATURES             Location/Qualifiers
     source          1..11417
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        614..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     768..788
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     862..881
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        862..880
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    915..939
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6786..6795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6790..6799
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6807..6830
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6867..6890
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6915..6938
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     promoter        complement(6969..6987)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6970..6987)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7007..7024)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7013..7237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7283..7711
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7370..7389)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7580..7601
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7720..7740)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7725..8054
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7905..8040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7967..7986
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8121..8915
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8175..8194)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8785..8804
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9089..9210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9126..9145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9180..9199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9259..9275)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9259..9275)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9272..9294)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9283..9299
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9307..9313,9314..9331,9332..9337))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9352..9373
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9490..9507)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9661..10249)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9741..9760)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10420..11211,11212..11280))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11043..11062
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11281..11385)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta gagaacccac
      841 tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag cttggtaccg
      901 agctcggatc catgccaact ttgtacaaaa aagttggcat ggcccctacc aaggtgacct
      961 tcggagacga caccgtgatc gaggtgcagg gttacaagag cgtgaacatc accttcgagc
     1021 tggacgaacg catcgacaag gtgctgaacg aaaagtgctc cgcttacacc gtggagctgg
     1081 gcaccgaggt gaacgaattc gcctgcgtgg tggccgacgc tgtgatcaag accctgcagc
     1141 cagtgtccga actgctgacc cctctgggta tcgacctgga cgagtggtct atggctacct
     1201 actacctgtt cgacgagagc ggcgaattca agctggcctc tcacatgtac tgcagcttct
     1261 accctcccga cgaagacgag gaagagggtg actgcgaaga ggaagagttc gagccttcca
     1321 ctcagtacga gtacggcacc gaagacgact accagggcaa gcctctggaa ttcggagcca
     1381 cctctgccgc tctgcagcct gaagaggaac aggaggaaga ctggctggac gacgactccc
     1441 agcagaccgt gggtcagcag gacggctctg aagacaacca gaccaccacc atccagacca
     1501 tcgtggaggt gcagcctcag ctggagatgg aactgacccc agtggtgcag accatcgagg
     1561 tgaacagctt ctccggctac ctgaagctga ccgacaacgt gtacatcaag aacgctgaca
     1621 tcgtggagga agccaagaag gtgaaaccaa ccgtggtggt gaacgccgct aacgtgtacc
     1681 tgaagcacgg cggaggtgtg gctggagctc tgaacaaggc taccaacaac gccatgcagg
     1741 tggaatccga cgactacatc gctaccaacg gtcctctgaa ggtgggcgga tcctgcgtgc
     1801 tgtctggcca caacctggcc aagcactgcc tgcacgtggt gggaccaaac gtgaacaagg
     1861 gagaggacat ccagctgctg aagagcgcct acgaaaactt caaccagcac gaggtgctgc
     1921 tggctccact gctgtctgcc ggaatcttcg gtgctgatcc catccacagc ctgcgcgtgt
     1981 gcgtggacac cgtgaggacc aacgtgtacc tggccgtgtt cgacaagaac ctgtacgaca
     2041 agctggtgag ctccttcctg gagatgaagt ccgaaaagca ggtggagcag aagatcgccg
     2101 agatccccaa ggaggaagtg aagcctttca tcaccgaatc taagcccagc gtggagcaga
     2161 gaaagcagga cgacaagaag atcaaggcct gcgtggagga agtgaccacc accctggagg
     2221 aaaccaagtt cctgaccgag aacctgctgc tgtacatcga catcaacggc aacctgcacc
     2281 ctgactccgc taccctggtg tctgacatcg acatcacctt cctgaagaag gacgccccct
     2341 acatcgtggg cgacgtggtg caggaaggag tgctgaccgc tgtggtcatc cctaccaaga
     2401 aggccggtgg caccaccgag atgctggcca aggctctgcg taaggtgcca accgacaact
     2461 acatcaccac ctaccccggt cagggcctga acggttacac cgtggaggaa gctaagaccg
     2521 tgctgaagaa gtgcaagtct gccttctaca tcctgccctc catcatctct aacgagaagc
     2581 aggaaatcct gggcaccgtg agctggaacc tgcgcgaaat gctggcccac gctgaggaaa
     2641 ccaggaagct gatgcccgtg tgcgtggaga ccaaggccat cgtgagcacc atccagagaa
     2701 agtacaaggg catcaagatc caggagggtg tggtggacta cggcgctcgt ttctacttct
     2761 acacctccaa gaccaccgtg gcctctctga tcaacaccct gaacgacctg aacgagaccc
     2821 tggtgaccat gccactggga tacgtgaccc acggtctgaa cctggaggaa gccgctagat
     2881 acatgcgttc cctgaaggtg cctgctaccg tgagcgtgag cagccctgac gctgtgaccg
     2941 cctacaacgg atacctgacc tcctctagca agacccctga ggaacacttc atcgaaacca
     3001 tcagcctggc cggttcctac aaggactggt cttacagcgg acagtccacc cagctgggca
     3061 tcgagttcct gaagcgcgga gacaagagcg tgtactacac ctccaaccca accaccttcc
     3121 acctggacgg tgaagtgatc accttcgaca acctgaagac cctgctgtct ctgcgcgagg
     3181 tgaggaccat caaggtgttc accaccgtgg acaacatcaa cctgcacacc caggtggtgg
     3241 acatgagcat gacctacgga cagcagttcg gtcctaccta cctggacgga gctgacgtga
     3301 ccaagatcaa gccacacaac tcccacgagg gcaagacctt ctacgtgctg cctaacgatg
     3361 acaccctgag agtggaggct ttcgaatact accacaccac cgacccatcc ttcctgggca
     3421 ggtacatgtc tgccctgaac cacaccaaga agtggaagta cccccaggtg aacggactga
     3481 ccagcatcaa gtgggccgac aacaactgct acctggccac cgctctgctg accctgcagc
     3541 agatcgaact gaagttcaac ccacccgctc tgcaggacgc ttactacaga gcccgtgctg
     3601 gcgaggccgc taacttctgc gctctgatcc tggcctactg caacaagacc gtgggcgaac
     3661 tgggagacgt gagagagacc atgagctacc tgttccagca cgccaacctg gactcctgca
     3721 agcgtgtgct gaacgtggtg tgcaagacct gcggccagca gcagaccacc ctgaagggag
     3781 tggaagctgt gatgtacatg ggtaccctgt cctacgagca gttcaagaag ggcgtgcaga
     3841 tcccttgcac ctgcggaaag caggccacca agtacctggt gcagcaggaa agcccattcg
     3901 tgatgatgtc cgctcctcca gcccagtacg aactgaagca cggcaccttc acctgcgctt
     3961 ccgagtacac cggcaactac cagtgcggac actacaagca catcacctct aaggagaccc
     4021 tgtactgcat cgacggagcc ctgctgacca agtcctctga atacaagggt cccatcaccg
     4081 acgtgttcta caaggagaac agctacacca ccaccatcaa gcccgtgacc tacaagctgg
     4141 acggcgtggt gtgcaccgaa atcgacccta agctggacaa ctactacaag aaggacaact
     4201 cttacttcac cgagcagcct atcgacctgg tgccaaacca gccctaccct aacgccagct
     4261 tcgacaactt caagttcgtg tgcgacaaca tcaagttcgc tgacgacctg aaccagctga
     4321 ccggatacaa gaagcctgcc tctagagagc tgaaggtgac cttcttccca gacctgaacg
     4381 gcgacgtggt ggctatcgac tacaagcact acaccccaag cttcaagaag ggagccaagc
     4441 tgctgcacaa gcccatcgtg tggcacgtga acaacgctac caacaaggcc acctacaagc
     4501 ctaacacctg gtgcatccgt tgcctgtggt ctaccaagcc agtggaaacc tccaactctt
     4561 tcgacgtgct gaagagcgag gacgctcagg gcatggacaa cctggcctgc gaagacctga
     4621 agcccgtgag cgaggaagtg gtggagaacc caaccatcca gaaggacgtg ctggaatgca
     4681 acgtgaagac caccgaggtg gtgggagaca tcatcctgaa gccagctaac aacagcctga
     4741 agatcaccga ggaagtgggt cacaccgacc tgatggccgc ttacgtggac aacagctccc
     4801 tgaccatcaa gaagcccaac gagctgagca gggtgctggg tctgaagacc ctggctactc
     4861 acggtctggc tgctgtgaac tctgtgccat gggacaccat cgctaactac gccaagcctt
     4921 tcctgaacaa ggtggtgtcc accaccacca acatcgtgac cagatgtctg aaccgcgtgt
     4981 gcaccaacta catgccctac ttcttcaccc tgctgctgca gctgtgcacc ttcaccagaa
     5041 gcaccaactc ccgtatcaag gctagcatgc ctaccaccat cgccaagaac accgtgaaga
     5101 gcgtgggaaa gttctgcctg gaggcttcct tcaactacct gaagtctcca aacttcagca
     5161 agctgatcaa catcatcatc tggttcctgc tgctctccgt gtgcctgggt tccctgatct
     5221 actctaccgc cgctctggga gtgctgatgt ctaacctggg tatgcctagc tactgcactg
     5281 gttacagaga gggatacctg aacagcacca acgtgaccat cgccacctac tgcaccggca
     5341 gcatcccatg ctccgtgtgc ctgtctggac tggacagcct ggacacctac ccctccctgg
     5401 aaaccatcca gatcaccatc tctagcttca agtgggacct gaccgccttc ggtctggtgg
     5461 ctgagtggtt cctggcctac atcctgttca ccaggttctt ctacgtgctg ggcctggccg
     5521 ctatcatgca gctgttcttc tcctacttcg ctgtgcactt catcagcaac tcctggctga
     5581 tgtggctgat catcaacctg gtgcagatgg ctcccatctc tgccatggtg cgcatgtaca
     5641 tcttcttcgc cagcttctac tacgtgtgga agtcctacgt gcacgtggtg gacggttgca
     5701 actcctctac ctgcatgatg tgctacaagc gcaacagggc taccagggtg gagtgcacca
     5761 ccatcgtgaa cggcgtgcgc aggtccttct acgtgtacgc caacggcggc aagggattct
     5821 gcaagctgca caactggaac tgcgtgaact gcgacacctt ctgcgctggc tccaccttca
     5881 tctctgacga ggtggccaga gacctgagcc tgcagttcaa gcgtccaatc aaccccaccg
     5941 accagagctc ctacatcgtg gactctgtga ccgtgaagaa cggtagcatc cacctgtact
     6001 tcgacaaggc tggccagaag acctacgagc gccactctct gagccacttc gtgaacctgg
     6061 acaacctgag ggccaacaac accaagggca gcctgcctat caacgtgatc gtgttcgacg
     6121 gaaagtccaa gtgcgaggaa tctagcgcca agtccgccag cgtgtactac agccagctga
     6181 tgtgccagcc aatcctgctg ctggaccagg ctctggtgtc tgacgtggga gacagcgccg
     6241 aagtggccgt gaagatgttc gacgcctacg tgaacacctt ctcctctacc ttcaacgtgc
     6301 ctatggagaa gctgaagacc ctggtggcta ctgctgaggc tgaactggct aagaacgtgt
     6361 ccctggacaa cgtgctgagc accttcatct ccgccgctag gcagggtttc gtggactctg
     6421 acgtggaaac caaagacgtg gtggagtgcc tgaagctgtc tcaccagagc gacatcgaag
     6481 tgaccggcga ctcctgcaac aactacatgc tgacctacaa caaggtggag aacatgaccc
     6541 caagagacct gggagcttgc atcgactgct ccgcccgtca catcaacgct caggtggcca
     6601 agtctcacaa catcgccctg atctggaacg tgaaggactt catgagcctg tccgagcagc
     6661 tgcgcaagca gatcaggagc gccgctaaga agaacaacct gcccttcaag ctgacctgcg
     6721 ctaccaccag acaggtggtg aacgtggtga ccaccaagat cgccctgaag ggcggagaat
     6781 tcgccgccac catggcggcc gccagctgga gccaccctca gttcgagaag ggaggaggaa
     6841 gcggcggagg cagcggagga ggaagctgga gccacccgca gttcgagaaa tgactcgagc
     6901 atgcatctag aggctggagc cacccgcagt tcgagaaatg actcgagcat gcatctagag
     6961 ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct
     7021 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     7081 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     7141 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     7201 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     7261 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     7321 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     7381 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     7441 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     7501 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7561 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     7621 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7681 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     7741 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7801 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7861 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7921 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7981 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8041 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
     8101 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     8161 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     8221 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     8281 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     8341 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     8401 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     8461 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     8521 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     8581 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     8641 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     8701 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     8761 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     8821 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     8881 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     8941 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     9001 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     9061 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     9121 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9181 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     9241 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     9301 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     9361 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9421 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9481 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9541 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9601 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9661 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9721 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9781 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9841 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9901 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9961 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10021 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10081 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    10141 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10201 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10261 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10321 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10381 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10441 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10501 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10561 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10621 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10681 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10741 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10801 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10861 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10921 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10981 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    11041 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    11101 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    11161 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11221 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11281 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11341 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11401 agtgccacct gacgtcg
//
