LOCUS       sequence-417476-        9089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GLG1 fused with 2Strep in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Li J, Gui Q, Liang FX, Sall J, Zhang Q, Duan Y, Dhabaria A, Askenazi
            M, Ueberheide B, Stapleford KA, Pagano M
  TITLE     The REEP5/TRAM1 complex binds SARS-CoV-2 NSP3 and promotes virus 
            replication.
  JOURNAL   J Virol. 2023 Sep 28:e0050723. doi: 10.1128/jvi.00507-23.
  PUBMED    37768083
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2023 Sep 28:e0050723. doi: 10.1128/jvi.00507-23."
COMMENT     Sequence Label: GLG1-2Strep
FEATURES             Location/Qualifiers
     source          1..9089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..43)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        215..594
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        595..798
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     843..862
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        843..861
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      891..900
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4439..4448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4443..4452
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4460..4483
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4520..4543
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4568..4591
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     promoter        complement(4622..4640)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4623..4640)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4660..4677)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4666..4890
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4936..5364
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5023..5042)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5233..5254
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5373..5393)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5378..5707
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5558..5693
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5620..5639
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5774..6568
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5828..5847)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6438..6457
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6742..6863
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6779..6798)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6833..6852
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6912..6928)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6912..6928)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6925..6947)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6936..6952
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6960..6966,6967..6984,6985..6990))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7005..7026
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7143..7160)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7314..7902)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7394..7413)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8073..8864,8865..8933))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8696..8715
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8934..9038)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
       61 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
      121 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
      181 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
      241 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      301 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      361 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      421 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      481 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      541 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      601 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      661 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      721 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      781 gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa
      841 attaatacga ctcactatag ggagacccaa gcttggtacc gagctcggat ccatggcggc
      901 gtgtggacgt gtacggagga tgttccgctt gtcggcggcg ctgcatctgc tgctgctatt
      961 cgcggccggg gccgagaaac tccccggcca gggcgtccac agccagggcc agggtcccgg
     1021 ggccaacttt gtgtccttcg tagggcaggc cggaggcggc ggcccggcgg gtcagcagct
     1081 gccccagctg cctcagtcat cgcagcttca gcagcaacag cagcagcagc aacagcaaca
     1141 gcagcctcag ccgccgcagc cgcctttccc ggcgggtggg cctccggccc ggcggggagg
     1201 agcgggggct ggtgggggct ggaagctggc ggaggaagag tcctgcaggg aggacgtgac
     1261 ccgcgtgtgc cctaagcaca cctggagcaa caacctggcg gtgctcgagt gcctgcagga
     1321 tgtgagggag cctgaaaatg aaatttcttc agactgcaat catttgttgt ggaattataa
     1381 gctgaaccta actacagatc ccaaatttga atctgtggcc agagaggttt gcaaatctac
     1441 tataacagag attaaagaat gtgctgatga accggttgga aaaggttaca tggtttcctg
     1501 cttggtggat caccgaggca acatcactga gtatcagtgt caccagtaca ttaccaagat
     1561 gacggccatc atttttagtg attaccgttt aatctgtggc ttcatggatg actgcaaaaa
     1621 tgacatcaac attctgaaat gtggcagtat tcggcttgga gaaaaggatg cacattcaca
     1681 aggtgaggtg gtatcatgct tggagaaagg cctggtgaaa gaagcagaag aaagagaacc
     1741 caagattcaa gtttctgaac tctgcaagaa agccattctc cgggtggctg agctgtcatc
     1801 ggatgacttt cacttagacc ggcatttata ttttgcttgc cgagatgatc gggagcgttt
     1861 ttgtgaaaat acacaagctg gtgagggcag agtgtataag tgcctcttta accataaatt
     1921 tgaagaatcc atgagtgaaa agtgtcgaga agcacttaca acccgccaaa agctgattgc
     1981 ccaggattat aaagtcagtt attcattggc caaatcctgt aaaagtgact tgaagaaata
     2041 ccggtgcaat gtggaaaacc ttccgcgatc gcgtgaagcc aggctctcct acttgttaat
     2101 gtgcctggag tcagctgtac acagagggcg acaagtcagc agtgagtgcc agggggagat
     2161 gctggattac cgacgcatgt tgatggaaga cttttctctg agccctgaga tcatcctaag
     2221 ctgtcggggg gagattgaac accattgttc cggattacat cgaaaagggc ggaccctaca
     2281 ctgtctgatg aaagtagttc gaggggagaa ggggaacctt ggaatgaact gccagcaggc
     2341 gcttcaaaca ctgattcagg agactgaccc tggtgcagat taccgcattg atcgagcttt
     2401 gaatgaagct tgtgaatctg taatccagac agcctgcaaa catataagat ctggagaccc
     2461 aatgatcttg tcgtgcctga tggaacattt atacacagag aagatggtag aagactgtga
     2521 acaccgtctc ttagagctgc agtatttcat ctcccgggat tggaagctgg accctgtcct
     2581 gtaccgcaag tgccagggag acgcttctcg tctttgccac acccacggtt ggaatgagac
     2641 cagtgaattt atgcctcagg gagctgtgtt ctcttgttta tacagacacg cctaccgcac
     2701 tgaggaacag ggaaggaggc tctcacggga gtgccgagct gaagtccaaa ggatcctaca
     2761 ccagcgtgcc atggatgtca agctggatcc tgccctccag gataagtgcc tgattgatct
     2821 gggaaaatgg tgcagtgaga aaacagagac tggacaggag ctggagtgcc ttcaggacca
     2881 tctggatgac ttggtggtgg agtgtagaga tatagttggc aacctcactg agttagaatc
     2941 agaggatatt caaatagaag ccttgctgat gagagcctgt gagcccataa ttcagaactt
     3001 ctgccacgat gtggcagata accagataga ctctggggac ctgatggagt gtctgataca
     3061 gaacaaacac cagaaggaca tgaacgagaa gtgtgccatc ggagttaccc acttccagct
     3121 ggtgcagatg aaggattttc ggttttctta caagtttaaa atggcctgca aggaggacgt
     3181 gttgaagctt tgcccaaaca taaaaaagaa ggtggacgtg gtgatctgcc tgagcacgac
     3241 cgtgcgcaat gacactctgc aggaagccaa ggagcacagg gtgtccctga agtgccgcag
     3301 gcagctccgt gtggaggagc tggagatgac ggaggacatc cgcttggagc cagatctata
     3361 cgaagcctgc aagagtgaca tcaaaaactt ctgttccgct gtgcaatatg gcaacgctca
     3421 gattatcgaa tgtctgaaag aaaacaagaa gcagctaagc acccgctgcc accaaaaagt
     3481 atttaagctg caggagacag agatgatgga cccagagcta gactacaccc tcatgagggt
     3541 ctgtaagcag atgataaaga ggttctgtcc ggaagcagat tctaaaacca tgttgcagtg
     3601 cttgaagcaa aataaaaaca gtgaattgat ggatcccaaa tgcaaacaga tgataaccaa
     3661 gcgccagatc acccagaaca cagattaccg cttaaacccc atgttaagaa aagcctgtaa
     3721 agctgacatt cctaaattct gtcacggtat cctgactaag gccaaggatg attcagaatt
     3781 agaaggacaa gtcatctctt gcctgaagct gagatatgct gaccagcgcc tgtcttcaga
     3841 ctgtgaagac cagatccgaa tcattatcca ggagtccgcc ctggactacc gcctggatcc
     3901 tcagctccag ctgcactgct cagacgagat ctccagtcta tgtgctgaag aagcagcagc
     3961 ccaagagcag acaggtcagg tggaggagtg cctcaaggtc aacctgctca agatcaaaac
     4021 agaattgtgt aaaaaggaag tgctaaacat gctgaaggaa agcaaagcag acatctttgt
     4081 tgacccggta cttcatactg cttgtgccct ggacattaaa caccactgcg cagccatcac
     4141 ccctggccgc gggcgtcaaa tgtcctgtct catggaagca ctggaggata agcgggtgag
     4201 gttacagccc gagtgcaaaa agcgcctcaa tgaccggatt gagatgtgga gttacgcagc
     4261 aaaggtggcc ccagcagatg gcttctctga tcttgccatg caagtaatga cgtctccatc
     4321 taagaactac attctctctg tgatcagtgg gagcatctgt atattgttcc tgattggcct
     4381 gatgtgtgga cggatcacca agcgagtgac acgagagctc aaggacaggg aattcgccgc
     4441 caccatggcg gccgccagct ggagccaccc tcagttcgag aagggaggag gaagcggcgg
     4501 aggcagcgga ggaggaagct ggagccaccc gcagttcgag aaatgactcg agcatgcatc
     4561 tagaggctgg agccacccgc agttcgagaa atgactcgag catgcatcta gagggcccta
     4621 ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt
     4681 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4741 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4801 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4861 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     4921 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4981 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5041 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5101 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5161 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5221 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5281 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5341 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     5401 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5461 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5521 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5581 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5641 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5701 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     5761 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5821 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     5881 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     5941 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     6001 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     6061 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     6121 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     6181 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     6241 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     6301 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     6361 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     6421 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     6481 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     6541 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     6601 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6661 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6721 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     6781 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6841 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     6901 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6961 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7021 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     7081 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     7141 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7201 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7261 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7321 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7381 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7441 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7501 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7561 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7621 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7681 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7741 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7801 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     7861 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     7921 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     7981 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     8041 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8101 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8161 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8221 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8281 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8341 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8401 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8461 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8521 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8581 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     8641 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8701 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8761 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8821 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8881 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8941 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     9001 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     9061 cctgacgtcg acggatcggg agatctccc
//
