LOCUS       sequence-417524-        9484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pQFT with mNeonGreen and sacB under control of PQJ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Klotz A, Kaczmarczyk A, Jenal U
  TITLE     A Synthetic Cumate-Inducible Promoter for Graded and Homogenous Gene
            Expression in Pseudomonas aeruginosa.
  JOURNAL   Appl Environ Microbiol. 2023 May 18:e0021123. doi: 
            10.1128/aem.00211-23.
  PUBMED    37199671
REFERENCE   2  (bases 1 to 9484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2023 May 18:e0021123. doi: 10.1128/aem.00211-23."
COMMENT     Sequence Label: pQFT-mNGsacB
FEATURES             Location/Qualifiers
     source          1..9484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      27..658
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1121..1709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1610..1629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1771..2382)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2459..2486
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2529..2556
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             join(3297..3383,3384..4718)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     primer_bind     complement(4787..4804)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4787..4803)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4796..4818)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5012..5031)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5131..5153
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5191..5209)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             5995..7194
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(7425..8573)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8845..9216)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            9249..9358
                     /note="incP origin of transfer"
ORIGIN
        1 gcggaaaaga tccgtcgacc ctttccgacg ctcaccgggc tggttgccct cgccgctggg
       61 ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga
      121 caccgcggcc gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga
      181 ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg
      241 caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc
      301 tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg
      361 tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag
      421 gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc
      481 agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt
      541 taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc
      601 gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa
      661 cgcgggcctc ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa
      721 aatggcagcc aagctgacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
      781 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
      841 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
      901 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
      961 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1021 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1081 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1141 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1201 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1261 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     1321 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     1381 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     1441 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     1501 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     1561 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     1621 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     1681 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     1741 tggccttttg ctggcctttt gctcacatgt tcagcgcttg aacttggcgt agcgctcgcg
     1801 ggcgatttcc agggtcgagt tgcggacgcg ctcgaagcgc tccttgtcct tctgccacag
     1861 cgagcggacc gccaggccgc ggaccgagtt gaagatcagc cacaggatgt cctccgcgtc
     1921 gtcgcggctc aggccgcgcg acaccagcac gcccagccac atatcctcga cgacgaagcg
     1981 gttgcgctcg acggtgcgct ggatgccctc gcgcagcgcc gggtcgcggt cggcggccac
     2041 gatcaggtcc agcgagatcg agaagtcgtc gtccaggaag aactcggcgg cgtcgtccag
     2101 catctgctgg atgacgtcgt cctcgggctt cagcttggcc aggcgggcgc ggctgcgctc
     2161 ggtgatctgc tcgtacagcc actcgaaggt ggccagcagc agttccagct tggtcgggaa
     2221 gtggtgcgac tgggcgccgc ggctgacgcc ggcggcgccc gggacgtcgg cgatgcggaa
     2281 gccggcatag cccttctcgc gcaggacgcc cagggcggcg gcgatcagct tgccctgggt
     2341 ttccatcgcg cgctcggcct gggtgcggcg cttcggcgac atgatgacca ttcggggtct
     2401 ctccctgcta gcatcagggt tattgtatca tgagcggata catatgtcaa tgtaccggaa
     2461 caaacagaca atctggtctg tttgtacaat attgacgccc tgtgggggca tccgtatgat
     2521 tgcgcctcaa caaacagaca atctggtctg tttgtaacta gtagaggaag cttccgcatg
     2581 gtgagcaaag gtgaagagga taatatggca agcctgcctg caacccatga actgcatatt
     2641 tttggtagca ttaacggcgt ggattttgat atggttggtc agggcaccgg taatccgaat
     2701 gatggttatg aagaactgaa tctgaaaagc accaaaggcg atctgcagtt tagcccgtgg
     2761 attctggttc cgcatattgg ttatggtttt catcagtatc tgccgtatcc ggatggtatg
     2821 agcccgtttc aggcagcaat ggttgatggt agcggttatc aggttcatcg taccatgcag
     2881 tttgaagatg gtgcaagcct gaccgttaat tatcgttata cctatgaagg cagccacatt
     2941 aaaggcgaag cacaggttaa aggcaccggt tttccggcag atggtccggt tatgaccaat
     3001 agtctgaccg cagcagattg gtgtcgtagc aaaaaaacct atccgaacga taaaaccatc
     3061 atcagcacct tcaaatggtc atataccacc ggcaatggta aacgttatcg tagcaccgca
     3121 cgtaccacct atacctttgc aaaaccgatg gcagcaaact atctgaaaaa tcagccgatg
     3181 tatgtgttcc gtaaaaccga actgaaacac agcaaaacag agctgaactt taaagaatgg
     3241 cagaaagcct ttaccgatgt gatgggtatg gatgagctgt ataaataagg taccaaatga
     3301 acatcaaaaa gtttgcaaaa caagcaacag tattaacctt tactaccgca ctgctggcag
     3361 gaggcgcaac tcaagcgttt gcgaaagaaa cgaaccaaaa gccatataag gaaacatacg
     3421 gcatttccca tattacacgc catgatatgc tgcaaatccc tgaacagcaa aaaaatgaaa
     3481 aatatcaagt tcctgagttc gattcgtcca caattaaaaa tatctcttct gcaaaaggcc
     3541 tggacgtttg ggacagctgg ccattacaaa acgctgacgg cactgtcgca aactatcacg
     3601 gctaccacat cgtctttgca ttagccggag atcctaaaaa tgcggatgac acatcgattt
     3661 acatgttcta tcaaaaagtc ggcgaaactt ctattgacag ctggaaaaac gctggccgcg
     3721 tctttaaaga cagcgacaaa ttcgatgcaa atgattctat cctaaaagac caaacacaag
     3781 aatggtcagg ttcagccaca tttacatctg acggaaaaat ccgtttattc tacactgatt
     3841 tctccggtaa acattacggc aaacaaacac tgacaactgc acaagttaat gtatcagcat
     3901 cagacagctc tttgaacatc aacggtgtag aggattataa atcaatcttt gacggtgacg
     3961 gaaaaacgta tcaaaacgta cagcagttca tcgatgaagg caactacagc tcaggcgaca
     4021 accatacgct gagagatcct cactacgtag aagataaagg ccacaaatac ttagtatttg
     4081 aagcaaacac tggaactgaa gatggctacc aaggcgaaga atctttattt aacaaagcat
     4141 actatggcaa aagcacatca ttcttccgtc aagaaagtca aaaacttctg caaagcgata
     4201 aaaaacgcac ggctgagtta gcaaacggcg ctctcggtat gattgagcta aacgatgatt
     4261 acacactgaa aaaagtgatg aaaccgctga ttgcatctaa cacagtaaca gatgaaattg
     4321 aacgcgcgaa cgtctttaaa atgaacggca aatggtatct gttcactgac tcccgcggat
     4381 caaaaatgac gattgacggc attacgtcta acgatattta catgcttggt tatgtttcta
     4441 attctttaac tggcccatac aagccgctga acaaaactgg ccttgtgtta aaaatggatc
     4501 ttgatcctaa cgatgtaacc tttacttact cacacttcgc tgtacctcaa gcgaaaggaa
     4561 acaatgtcgt gattacaagc tatatgacaa acagaggatt ctacgcagac aaacaatcaa
     4621 cgtttgcgcc gagcttcctg ctgaacatca aaggcaagaa aacatctgtt gtcaaagaca
     4681 gcatccttga acaaggacaa ttaacagtta ataaataagg atccggtgaa gtgacccgca
     4741 aaaaaaaagg ccaggggatc gctcccccgg cctttttttt gaattcactg gccgtcgttt
     4801 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     4861 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     4921 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     4981 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     5041 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     5101 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     5161 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     5221 atgtcatgat aataatggtt tcttagcacc ctttctcggt ccttcaacgt tcctgacaac
     5281 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     5341 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     5401 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     5461 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     5521 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     5581 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     5641 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     5701 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     5761 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     5821 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     5881 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     5941 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     6001 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     6061 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     6121 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     6181 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     6241 gccactgtcg actacgccat catggcgaca gcgcctttcc tttgggttct ctatatcggg
     6301 cggatcgtgg ccggcatcac cggggcgact ggggcggtag ccggcgctta tattgccgat
     6361 atcactgatg gcgatgagcg cgcgcggcac ttcggcttca tgagcgcctg tttcgggttc
     6421 gggatggtcg cgggacctgt gctcggtggg ctgatgggcg gtttctcccc ccacgctccg
     6481 ttcttcgccg cggcagcctt gaacggcctc aatttcctga cgggctgttt ccttttgccg
     6541 gagtcgcaca aaggcgaacg ccggccgtta cgccgggagg ctctcaaccc gctcgcttcg
     6601 ttccggtggg cccggggcat gaccgtcgtc gccgccctga tggcggtctt cttcatcatg
     6661 caacttgtcg gacaggtgcc ggccgcgctt tgggtcattt tcggcgagga tcgctttcac
     6721 tgggacgcga ccacgatcgg catttcgctt gccgcatttg gcattctgca ttcactcgcc
     6781 caggcaatga tcaccggccc tgtagccgcc cggctcggcg aaaggcgggc actcatgctc
     6841 ggaatgattg ccgacggcac aggctacatc ctgcttgcct tcgcgacacg gggatggatg
     6901 gcgttcccga tcatggtcct gcttgcttcg ggtggcatcg gaatgccggc gctgcaagca
     6961 atgttgtcca ggcaggtgga tgaggaacgt caggggcagc tgcaaggctc actggcggcg
     7021 ctcaccagcc tgacctcgat cgtcggaccc ctcctcttca cggcgatcta tgcggcttct
     7081 ataacaacgt ggaacgggtg ggcatggatt gcaggcgctg ccctctactt gctctgcctg
     7141 ccggcgctgc gtcgcgggct ttggagcggc gcagggcaac gagccgatcg ctgatcgtgg
     7201 aaacgatagg cctatgccat gcgggtcaag gcgacttccg gcaagctata cgcgccctag
     7261 aattgtcaat tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt gcgtcccgag
     7321 cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa tgccagtaaa
     7381 gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc
     7441 accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag
     7501 gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg
     7561 cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc
     7621 cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg
     7681 ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg
     7741 ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg
     7801 tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg
     7861 taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg
     7921 atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg
     7981 tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg
     8041 aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg
     8101 gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg
     8161 aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc
     8221 ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc
     8281 atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga
     8341 cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg
     8401 ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga ctggaaggtt
     8461 tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac
     8521 cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct
     8581 ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg atttgacccg
     8641 cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt cccgtagacc
     8701 gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa taccagctcc
     8761 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
     8821 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
     8881 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
     8941 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
     9001 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
     9061 cgtgattttg tagccctggc cgacggccag caggtaggcc tacaggctca tgccggccgc
     9121 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
     9181 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
     9241 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
     9301 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
     9361 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
     9421 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
     9481 tgca
//
