LOCUS       sequence-417526-       10405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pQFT with lacZ under control of PQJ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Klotz A, Kaczmarczyk A, Jenal U
  TITLE     A Synthetic Cumate-Inducible Promoter for Graded and Homogenous Gene
            Expression in Pseudomonas aeruginosa.
  JOURNAL   Appl Environ Microbiol. 2023 May 18:e0021123. doi: 
            10.1128/aem.00211-23.
  PUBMED    37199671
REFERENCE   2  (bases 1 to 10405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2023 May 18:e0021123. doi: 10.1128/aem.00211-23."
COMMENT     Sequence Label: pQFT-lacZ
FEATURES             Location/Qualifiers
     source          1..10405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      31..662
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1125..1713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1614..1633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2463..2490
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2533..2560
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     primer_bind     2587..2603
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2587..2603
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2599..5673
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     complement(2619..2635)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5712..5728)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5937..5956)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6056..6078
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6116..6134)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             6920..8119
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(8350..9498)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9770..10141)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            10174..10283
                     /note="incP origin of transfer"
ORIGIN
        1 tgcagcggaa aagatccgtc gaccctttcc gacgctcacc gggctggttg ccctcgccgc
       61 tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc
      121 gagacaccgc ggccgccggc gttgtggata cctcgcggaa aacttggccc tcactgacag
      181 atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt tgacagatga
      241 ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa
      301 cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga taagtgccct
      361 gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat ccttgacact
      421 tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg ggctgtccac
      481 aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc gctaacctgt
      541 cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg gctgcgccct
      601 gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc tcccggcccg
      661 ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc
      721 caaaaatggc agccaagctg accacttctg cgctcggccc ttccggctgg ctggtttatt
      781 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
      841 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
      901 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
      961 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1021 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1081 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1141 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1201 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1261 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1321 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1381 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     1441 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     1501 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     1561 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     1621 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     1681 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     1741 ttcctggcct tttgctggcc ttttgctcac atgttcagcg cttgaacttg gcgtagcgct
     1801 cgcgggcgat ttccagggtc gagttgcgga cgcgctcgaa gcgctccttg tccttctgcc
     1861 acagcgagcg gaccaccagg ccgcggaccg agttgaagat cagccacagg atgtcctccg
     1921 cgtcgtcgcg gctcaggccg cgcgacacca gcacgcccag ccacatatcc tcgacgacga
     1981 agcggttgcg ctcgacggtg cgctggatgc cctcgcgcag cgccgggtcg cggtcggcgg
     2041 ccacgatcag gtccaggccg atcgagaagt cgtcgtccag gaagaactcg gcggcgtcgt
     2101 ccagcatctg ctggatgacg tcgtcctcgg gcttcagctt ggccaggcgg gcgcggctgc
     2161 gctcggtgat ctgctcgtac agccactcga aggtggccag cagcagttcc agcttggtcg
     2221 ggaagtggtg cgactgggcg ccgcggctga cgccggcggc gcccgggacg tcggcgatgc
     2281 ggaagccggc atagcccttc tcgcgcagga cgcccagggc ggcggcgatc agcttgccct
     2341 gggtttccat cgcgcgctcg gcctgggtgc ggcgcttcgg cgacatgatg accattcggg
     2401 gtctctccct gctagcatca gggttattgt atcatgagcg gatacatatg tcaatgtacc
     2461 ggaacaaaca gacaatctgg tctgtttgta caatattgac gccctgtggg ggcatccgta
     2521 tgattgcgcc tcaacaaaca gacaatctgg tctgtttgta actagagggc cggatcccgg
     2581 tacccacagg aaacagctat gaccatgatt acggattcac tggccgtcgt tttacaacgt
     2641 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     2701 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     2761 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
     2821 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac
     2881 ggttacgatg cgcccatcta caccaacgtg acctatccca ttacggtcaa tccgccgttt
     2941 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg
     3001 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg
     3061 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg
     3121 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac
     3181 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg
     3241 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat
     3301 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac
     3361 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct
     3421 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg
     3481 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt
     3541 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc
     3601 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc
     3661 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg
     3721 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg
     3781 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc
     3841 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta
     3901 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg
     3961 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc
     4021 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc
     4081 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg
     4141 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg
     4201 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg
     4261 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag
     4321 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc
     4381 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg
     4441 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg
     4501 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac
     4561 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct
     4621 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa
     4681 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac
     4741 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg
     4801 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat
     4861 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa
     4921 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt
     4981 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg
     5041 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg
     5101 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg
     5161 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg
     5221 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag
     5281 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc
     5341 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc
     5401 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc
     5461 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc
     5521 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt
     5581 ggtggcgacg actcctggag cccgtcagta tcggcggaat tacagctgag cgccggtcgc
     5641 taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc
     5701 gtattgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5761 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     5821 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     5881 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     5941 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     6001 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     6061 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     6121 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gcaccctttc
     6181 tcggtccttc aacgttcctg acaacgagcc tccttttcgc caatccatcg acaatcaccg
     6241 cgagtccctg ctcgaacgct gcgtccggac cggcttcgtc gaaggcgtct atcgcggccc
     6301 gcaacagcgg cgagagcgga gcctgttcaa cggtgccgcc gcgctcgccg gcatcgctgt
     6361 cgccggcctg ctcctcaagc acggccccaa cagtgaagta gctgattgtc atcagcgcat
     6421 tgacggcgtc cccggccgaa aaacccgcct cgcagaggaa gcgaagctgc gcgtcggccg
     6481 tttccatctg cggtgcgccc ggtcgcgtgc cggcatggat gcgcgcgcca tcgcggtagg
     6541 cgagcagcgc ctgcctgaag ctgcgggcat tcccgatcag aaatgagcgc cagtcgtcgt
     6601 cggctctcgg caccgaatgc gtatgattct ccgccagcat ggcttcggcc agtgcgtcga
     6661 gcagcgcccg cttgttcctg aagtgccagt aaagcgccgg ctgctgaacc cccaaccgtt
     6721 ccgccagttt gcgtgtcgtc agaccgtcta cgccgacctc gttcaacagg tccagggcgg
     6781 cacggatcac tgtattcggc tgcaactttg tcatgcttga cactttatca ctgataaaca
     6841 taatatgtcc accaacttat cagtgataaa gaatccgcgc gttcaatcgg accagcggag
     6901 gctggtccgg aggccagacg tgaaacccaa catacccctg atcgtaattc tgagcactgt
     6961 cgcgctcgac gctgtcggca tcggcctgat tatgccggtg ctgccgggcc tcctgcgcga
     7021 tctggttcac tcgaacgacg tcaccgccca ctatggcatt ctgctggcgc tgtatgcgtt
     7081 ggtgcaattt gcctgcgcac ctgtgctggg cgcgctgtcg gatcgtttcg ggcggcggcc
     7141 aatcttgctc gtctcgctgg ccggcgccac tgtcgactac gccatcatgg cgacagcgcc
     7201 tttcctttgg gttctctata tcgggcggat cgtggccggc atcaccgggg cgactggggc
     7261 ggtagccggc gcttatattg ccgatatcac tgatggcgat gagcgcgcgc ggcacttcgg
     7321 cttcatgagc gcctgtttcg ggttcgggat ggtcgcggga cctgtgctcg gtgggctgat
     7381 gggcggtttc tccccccacg ctccgttctt cgccgcggca gccttgaacg gcctcaattt
     7441 cctgacgggc tgtttccttt tgccggagtc gcacaaaggc gaacgccggc cgttacgccg
     7501 ggaggctctc aacccgctcg cttcgttccg gtgggcccgg ggcatgaccg tcgtcgccgc
     7561 cctgatggcg gtcttcttca tcatgcaact tgtcggacag gtgccggccg cgctttgggt
     7621 cattttcggc gaggatcgct ttcactggga cgcgaccacg atcggcattt cgcttgccgc
     7681 atttggcatt ctgcattcac tcgcccaggc aatgatcacc ggccctgtag ccgcccggct
     7741 cggcgaaagg cgggcactca tgctcggaat gattgccgac ggcacaggct acatcctgct
     7801 tgccttcgcg acacggggat ggatggcgtt cccgatcatg gtcctgcttg cttcgggtgg
     7861 catcggaatg ccggcgctgc aagcaatgtt gtccaggcag gtggatgagg aacgtcaggg
     7921 gcagctgcaa ggctcactgg cggcgctcac cagcctgacc tcgatcgtcg gacccctcct
     7981 cttcacggcg atctatgcgg cttctataac aacgtggaac gggtgggcat ggattgcagg
     8041 cgctgccctc tacttgctct gcctgccggc gctgcgtcgc gggctttgga gcggcgcagg
     8101 gcaacgagcc gatcgctgat cgtggaaacg ataggcctat gccatgcggg tcaaggcgac
     8161 ttccggcaag ctatacgcgc cctagaattg tcaattttaa tcctctgttt atcggcagtt
     8221 cgtagagcgc gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc
     8281 cgcttgttcc tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg gaactgaccc
     8341 cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag
     8401 gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg
     8461 ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg
     8521 gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc
     8581 ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag
     8641 gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga
     8701 caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg
     8761 cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg
     8821 gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc
     8881 caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt
     8941 gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat
     9001 tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat
     9061 cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt
     9121 cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc
     9181 ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc
     9241 caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg
     9301 cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac
     9361 catcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat
     9421 ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg
     9481 gtcaaacgtc cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat
     9541 gtgcccttat tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc
     9601 ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat
     9661 cttgccctgc acgaatacca gctccgcgaa gtcgctcttc ttgatggagc gcatggggac
     9721 gtgcttggca atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt catggctctg
     9781 ccctcgggcg gaccacgccc atcatgacct tgccaagctc gtcctgcttc tcttcgatct
     9841 tcgccagcag ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg tcgtcggtga
     9901 gccagagttt cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg gccagctcgc
     9961 ggacgtgctc atagtccacg acgcccgtga ttttgtagcc ctggccgacg gccagcaggt
    10021 aggcctacag gctcatgccg gccgccgccg ccttttcctc aatcgctctt cgttcgtctg
    10081 gaaggcagta caccttgata ggtgggctgc ccttcctggt tggcttggtt tcatcagcca
    10141 tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga gcaggattcc
    10201 cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg
    10261 ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac
    10321 gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg
    10381 ctataatgac cccgaagcag ggtta
//
