LOCUS       sequence-417529-        9484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pQF with mNeonGreen and sacB under control of PQ5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Klotz A, Kaczmarczyk A, Jenal U
  TITLE     A Synthetic Cumate-Inducible Promoter for Graded and Homogenous Gene
            Expression in Pseudomonas aeruginosa.
  JOURNAL   Appl Environ Microbiol. 2023 May 18:e0021123. doi: 
            10.1128/aem.00211-23.
  PUBMED    37199671
REFERENCE   2  (bases 1 to 9484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2023 May 18:e0021123. doi: 10.1128/aem.00211-23."
COMMENT     Sequence Label: pQF-mNGsacB
FEATURES             Location/Qualifiers
     source          1..9484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      13..644
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1107..1695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1596..1615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1757..2368)
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     protein_bind    2445..2472
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    2515..2542
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     CDS             join(3283..3369,3370..4704)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     primer_bind     complement(4773..4790)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4773..4789)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4782..4804)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4998..5017)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5117..5139
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5177..5195)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             5981..7180
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(7411..8559)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8831..9202)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            9235..9344
                     /note="incP origin of transfer"
ORIGIN
        1 tcgacccttt ccgacgctca ccgggctggt tgccctcgcc gctgggctgg cggccgtcta
       61 tggccctgca aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg
      121 gcgttgtgga tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga
      181 cacttgaggg gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg
      241 gccggcgacg tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag
      301 tttcccacag atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag
      361 gggcgcgact actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga
      421 cagatgaggg gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat
      481 ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa
      541 accaatattt ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac
      601 gccgaagggg ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg ggcctcccat
      661 ccccccaggg gctgcgcccc tcggccgcga acggcctcac cccaaaaatg gcagccaagc
      721 tgaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      781 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      841 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      901 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
      961 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1021 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1081 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1141 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1201 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1261 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1321 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1381 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1441 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1501 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1561 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1621 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1681 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1741 ccttttgctc acatgttcag cgcttgaact tggcgtagcg ctcgcgggcg atttccaggg
     1801 tcgagttgcg gacgcgctcg aagcgctcct tgtccttctg ccacagcgag cggaccgcca
     1861 ggccgcggac cgagttgaag atcagccaca ggatgtcctc cgcgtcgtcg cggctcaggc
     1921 cgcgcgacac cagcacgccc agccacatat cctcgacgac gaagcggttg cgctcgacgg
     1981 tgcgctggat gccctcgcgc agcgccgggt cgcggtcggc ggccacgatc aggtccagcg
     2041 agatcgagaa gtcgtcgtcc aggaagaact cggcggcgtc gtccagcatc tgctggatga
     2101 cgtcgtcctc gggcttcagc ttggccaggc gggcgcggct gcgctcggtg atctgctcgt
     2161 acagccactc gaaggtggcc agcagcagtt ccagcttggt cgggaagtgg tgcgactggg
     2221 cgccgcggct gacgccggcg gcgcccggga cgtcggcgat gcggaagccg gcatagccct
     2281 tctcgcgcag gacgcccagg gcggcggcga tcagcttgcc ctgggtttcc atcgcgcgct
     2341 cggcctgggt gcggcgcttc ggcgacatga tgaccattcg gggtctctcc ctgctagcat
     2401 cagggttatt gtatcatgag cggatacata tgtcaatgta ccggaacaaa cagacaatct
     2461 ggtctgtttg tacatggttg acggatcgcc gcgaagccgc taactgcgcc ggttaacaaa
     2521 cagacaatct ggtctgtttg taactagtag aggaagcttc cgcatggtga gcaaaggtga
     2581 agaggataat atggcaagcc tgcctgcaac ccatgaactg catatttttg gtagcattaa
     2641 cggcgtggat tttgatatgg ttggtcaggg caccggtaat ccgaatgatg gttatgaaga
     2701 actgaatctg aaaagcacca aaggcgatct gcagtttagc ccgtggattc tggttccgca
     2761 tattggttat ggttttcatc agtatctgcc gtatccggat ggtatgagcc cgtttcaggc
     2821 agcaatggtt gatggtagcg gttatcaggt tcatcgtacc atgcagtttg aagatggtgc
     2881 aagcctgacc gttaattatc gttataccta tgaaggcagc cacattaaag gcgaagcaca
     2941 ggttaaaggc accggttttc cggcagatgg tccggttatg accaatagtc tgaccgcagc
     3001 agattggtgt cgtagcaaaa aaacctatcc gaacgataaa accatcatca gcaccttcaa
     3061 atggtcatat accaccggca atggtaaacg ttatcgtagc accgcacgta ccacctatac
     3121 ctttgcaaaa ccgatggcag caaactatct gaaaaatcag ccgatgtatg tgttccgtaa
     3181 aaccgaactg aaacacagca aaacagagct gaactttaaa gaatggcaga aagcctttac
     3241 cgatgtgatg ggtatggatg agctgtataa ataaggtacc aaatgaacat caaaaagttt
     3301 gcaaaacaag caacagtatt aacctttact accgcactgc tggcaggagg cgcaactcaa
     3361 gcgtttgcga aagaaacgaa ccaaaagcca tataaggaaa catacggcat ttcccatatt
     3421 acacgccatg atatgctgca aatccctgaa cagcaaaaaa atgaaaaata tcaagttcct
     3481 gagttcgatt cgtccacaat taaaaatatc tcttctgcaa aaggcctgga cgtttgggac
     3541 agctggccat tacaaaacgc tgacggcact gtcgcaaact atcacggcta ccacatcgtc
     3601 tttgcattag ccggagatcc taaaaatgcg gatgacacat cgatttacat gttctatcaa
     3661 aaagtcggcg aaacttctat tgacagctgg aaaaacgctg gccgcgtctt taaagacagc
     3721 gacaaattcg atgcaaatga ttctatccta aaagaccaaa cacaagaatg gtcaggttca
     3781 gccacattta catctgacgg aaaaatccgt ttattctaca ctgatttctc cggtaaacat
     3841 tacggcaaac aaacactgac aactgcacaa gttaatgtat cagcatcaga cagctctttg
     3901 aacatcaacg gtgtagagga ttataaatca atctttgacg gtgacggaaa aacgtatcaa
     3961 aacgtacagc agttcatcga tgaaggcaac tacagctcag gcgacaacca tacgctgaga
     4021 gatcctcact acgtagaaga taaaggccac aaatacttag tatttgaagc aaacactgga
     4081 actgaagatg gctaccaagg cgaagaatct ttatttaaca aagcatacta tggcaaaagc
     4141 acatcattct tccgtcaaga aagtcaaaaa cttctgcaaa gcgataaaaa acgcacggct
     4201 gagttagcaa acggcgctct cggtatgatt gagctaaacg atgattacac actgaaaaaa
     4261 gtgatgaaac cgctgattgc atctaacaca gtaacagatg aaattgaacg cgcgaacgtc
     4321 tttaaaatga acggcaaatg gtatctgttc actgactccc gcggatcaaa aatgacgatt
     4381 gacggcatta cgtctaacga tatttacatg cttggttatg tttctaattc tttaactggc
     4441 ccatacaagc cgctgaacaa aactggcctt gtgttaaaaa tggatcttga tcctaacgat
     4501 gtaaccttta cttactcaca cttcgctgta cctcaagcga aaggaaacaa tgtcgtgatt
     4561 acaagctata tgacaaacag aggattctac gcagacaaac aatcaacgtt tgcgccgagc
     4621 ttcctgctga acatcaaagg caagaaaaca tctgttgtca aagacagcat ccttgaacaa
     4681 ggacaattaa cagttaataa ataaggatcc ggtgaagtga cccgcaaaaa aaaaggccag
     4741 gggatcgctc ccccggcctt ttttttgaat tcactggccg tcgttttaca acgtcgtgac
     4801 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     4861 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4921 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     4981 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     5041 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     5101 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     5161 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5221 atggtttctt agcacccttt ctcggtcctt caacgttcct gacaacgagc ctccttttcg
     5281 ccaatccatc gacaatcacc gcgagtccct gctcgaacgc tgcgtccgga ccggcttcgt
     5341 cgaaggcgtc tatcgcggcc cgcaacagcg gcgagagcgg agcctgttca acggtgccgc
     5401 cgcgctcgcc ggcatcgctg tcgccggcct gctcctcaag cacggcccca acagtgaagt
     5461 agctgattgt catcagcgca ttgacggcgt ccccggccga aaaacccgcc tcgcagagga
     5521 agcgaagctg cgcgtcggcc gtttccatct gcggtgcgcc cggtcgcgtg ccggcatgga
     5581 tgcgcgcgcc atcgcggtag gcgagcagcg cctgcctgaa gctgcgggca ttcccgatca
     5641 gaaatgagcg ccagtcgtcg tcggctctcg gcaccgaatg cgtatgattc tccgccagca
     5701 tggcttcggc cagtgcgtcg agcagcgccc gcttgttcct gaagtgccag taaagcgccg
     5761 gctgctgaac ccccaaccgt tccgccagtt tgcgtgtcgt cagaccgtct acgccgacct
     5821 cgttcaacag gtccagggcg gcacggatca ctgtattcgg ctgcaacttt gtcatgcttg
     5881 acactttatc actgataaac ataatatgtc caccaactta tcagtgataa agaatccgcg
     5941 cgttcaatcg gaccagcgga ggctggtccg gaggccagac gtgaaaccca acatacccct
     6001 gatcgtaatt ctgagcactg tcgcgctcga cgctgtcggc atcggcctga ttatgccggt
     6061 gctgccgggc ctcctgcgcg atctggttca ctcgaacgac gtcaccgccc actatggcat
     6121 tctgctggcg ctgtatgcgt tggtgcaatt tgcctgcgca cctgtgctgg gcgcgctgtc
     6181 ggatcgtttc gggcggcggc caatcttgct cgtctcgctg gccggcgcca ctgtcgacta
     6241 cgccatcatg gcgacagcgc ctttcctttg ggttctctat atcgggcgga tcgtggccgg
     6301 catcaccggg gcgactgggg cggtagccgg cgcttatatt gccgatatca ctgatggcga
     6361 tgagcgcgcg cggcacttcg gcttcatgag cgcctgtttc gggttcggga tggtcgcggg
     6421 acctgtgctc ggtgggctga tgggcggttt ctccccccac gctccgttct tcgccgcggc
     6481 agccttgaac ggcctcaatt tcctgacggg ctgtttcctt ttgccggagt cgcacaaagg
     6541 cgaacgccgg ccgttacgcc gggaggctct caacccgctc gcttcgttcc ggtgggcccg
     6601 gggcatgacc gtcgtcgccg ccctgatggc ggtcttcttc atcatgcaac ttgtcggaca
     6661 ggtgccggcc gcgctttggg tcattttcgg cgaggatcgc tttcactggg acgcgaccac
     6721 gatcggcatt tcgcttgccg catttggcat tctgcattca ctcgcccagg caatgatcac
     6781 cggccctgta gccgcccggc tcggcgaaag gcgggcactc atgctcggaa tgattgccga
     6841 cggcacaggc tacatcctgc ttgccttcgc gacacgggga tggatggcgt tcccgatcat
     6901 ggtcctgctt gcttcgggtg gcatcggaat gccggcgctg caagcaatgt tgtccaggca
     6961 ggtggatgag gaacgtcagg ggcagctgca aggctcactg gcggcgctca ccagcctgac
     7021 ctcgatcgtc ggacccctcc tcttcacggc gatctatgcg gcttctataa caacgtggaa
     7081 cgggtgggca tggattgcag gcgctgccct ctacttgctc tgcctgccgg cgctgcgtcg
     7141 cgggctttgg agcggcgcag ggcaacgagc cgatcgctga tcgtggaaac gataggccta
     7201 tgccatgcgg gtcaaggcga cttccggcaa gctatacgcg ccctagaatt gtcaatttta
     7261 atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa
     7321 gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga
     7381 acccccagcc ggaactgacc ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt
     7441 gacccaggcg tgttccacca ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg
     7501 ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac atgaggcgga aggtttccag
     7561 cttgagcggg tacggctccc ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg
     7621 cgacagcttg cggtacttct cccatatgaa tttcgtgtag tggtcgccag caaacagcac
     7681 gacgatttcc tcgtcgatca ggacctggca acgggacgtt ttcttgccac ggtccaggac
     7741 gcggaagcgg tgcagcagcg acaccgattc caggtgccca acgcggtcgg acgtgaagcc
     7801 catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat accggccatt
     7861 gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat
     7921 aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc agttcgtcat cgtcggcccg
     7981 cagctcgacg ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt
     8041 ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt
     8101 gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg
     8161 catttccttg atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac
     8221 ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt
     8281 gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc
     8341 cacggcggcc gatggcgcgg gcagggcagg gggagccagt tgcacgctgt cgcgctcgat
     8401 cttggccgta gcttgctgga ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg
     8461 catgacggtg cggcttgcga tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag
     8521 ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt caccctcctt gcgggattgc
     8581 cccgactcac gccggggcaa tgtgccctta ttcctgattt gacccgcctg gtgccttggt
     8641 gtccagataa tccaccttat cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt
     8701 ctcgtacttg gtattccgaa tcttgccctg cacgaatacc agctccgcga agtcgctctt
     8761 cttgatggag cgcatgggga cgtgcttggc aatcacgcgc accccccggc cgttttagcg
     8821 gctaaaaaag tcatggctct gccctcgggc ggaccacgcc catcatgacc ttgccaagct
     8881 cgtcctgctt ctcttcgatc ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg
     8941 ccgtgcgcgg gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc
     9001 cattgatgcg ggccagctcg cggacgtgct catagtccac gacgcccgtg attttgtagc
     9061 cctggccgac ggccagcagg taggcctaca ggctcatgcc ggccgccgcc gccttttcct
     9121 caatcgctct tcgttcgtct ggaaggcagt acaccttgat aggtgggctg cccttcctgg
     9181 ttggcttggt ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc
     9241 agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga
     9301 aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga
     9361 tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg
     9421 atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaaga
     9481 tccg
//
