LOCUS       sequence-417569-        8927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses XPO6 fused with 2Strep in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Li J, Gui Q, Liang FX, Sall J, Zhang Q, Duan Y, Dhabaria A, Askenazi
            M, Ueberheide B, Stapleford KA, Pagano M
  TITLE     The REEP5/TRAM1 complex binds SARS-CoV-2 NSP3 and promotes virus 
            replication.
  JOURNAL   J Virol. 2023 Sep 28:e0050723. doi: 10.1128/jvi.00507-23.
  PUBMED    37768083
REFERENCE   2  (bases 1 to 8927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2023 Sep 28:e0050723. doi: 10.1128/jvi.00507-23."
COMMENT     Sequence Label: XPO6-2Strep
FEATURES             Location/Qualifiers
     source          1..8927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..57)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        229..608
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        609..812
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     763..783
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     857..876
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        857..875
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4291..4300
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      4295..4304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4312..4335
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4372..4395
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4420..4443
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     promoter        complement(4474..4492)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4475..4492)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4512..4529)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4518..4742
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4788..5216
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4875..4894)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5085..5106
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5225..5245)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5230..5559
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5410..5545
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5472..5491
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5626..6420
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5680..5699)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6290..6309
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6594..6715
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6631..6650)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6685..6704
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6764..6780)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6764..6780)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6777..6799)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6788..6804
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6812..6818,6819..6836,6837..6842))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6857..6878
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6995..7012)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7166..7754)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7246..7265)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7925..8716,8717..8785))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8548..8567
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8786..8890)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
       61 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
      121 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
      181 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
      241 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
      301 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      361 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      421 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      481 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      541 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      601 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      661 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      721 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      781 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt
      841 actggcttat cgaaattaat acgactcact atagggagac ccaagcttgg taccgagctc
      901 ggatccatgg catctgaaga agcctctctc agggcattgg aaagtctgat gacagaattt
      961 tttcacgatt gtacaaccaa tgaaagaaaa cgtgagatag aggagcttct taataacttt
     1021 gcccagcaaa taggagcctg gagattctgc ctgtactttc tctccagcac taggaatgac
     1081 tatgtaatga tgtacagttt aacagttttt gagaatctga tcaataaaat gtggcttggg
     1141 gtcccatctc aggataagat ggaaatccgt agctgtctgc ccaaactcct tttggctcac
     1201 cataaaacct taccttactt tatccggaac aagctctgca aagttattgt tgatattgga
     1261 cgtcaggatt ggcccatgtt ctaccacgac ttttttacta acattttaca gttgatccag
     1321 tcccctgtga caacccccct tgggctgatc atgttgaaga caacttcaga agagctggct
     1381 tgtccccgtg aggacctcag tgtggctcgg aaggaggagt tgcggaagct gctactggac
     1441 caggtgcaga cagtgcttgg gctactgaca ggtatcttgg agactgtctg ggacaaacac
     1501 agtgttactg ctgccactcc accaccatcc ccgacctcag gagaaagtgg tgacttactg
     1561 agtaacctgt tgcagagtcc cagttcagcc aaactgttga atcagccaat tcccatcctt
     1621 gatgtggaga gtgagtatat ctgttccctg gctttggagt gcctggccca tctcttcagt
     1681 tggattcctc tgtctgccag catcacccca tccctcctta ccaccatctt ccactttgca
     1741 cgatttggct gtgacatccg ggccagaaag atggcgtcag ttaacggcag cagccagaac
     1801 tgtgtctcgg gtcaggagcg cggccggctg ggggtcctgg ccatgtcctg catcaatgaa
     1861 ctcatgtcca agaactgtgt gcctatggaa tttgaggagt atttactgcg tatgttccag
     1921 cagactttct acctcctgca gaaaatcacc aaggataaca atgcccacac agtgaagagc
     1981 aggctagaag agctcgatga gagctatatc gagaagttta ctgactttct tcggctcttt
     2041 gtgagtgttc acctaagaag aatcgagtct tattcccagt tccctgtggt ggagtttttg
     2101 acacttttgt tcaagtacac atttcatcag cctactcatg aaggttactt ctcttgtttg
     2161 gatatctgga cgctgttttt ggactatctg acaagtaaaa ttaaaagtcg tcttggagac
     2221 aaggaagcag ttctcaacag gtacgaagat gccctggtgc tcctgctcac agaggtgttg
     2281 aatcgaatcc agttcagata caaccaagcc cagctggagg agttggatga tgagactctg
     2341 gatgacgatc agcagacgga gtggcagcgg tacttacggc agagcttgga ggtggtggcc
     2401 aaagtgatgg agctcctgcc cacgcacgcc ttctccacac tgttccctgt tcttcaggac
     2461 aatttagaag tttatttggg attacaacag tttatagtca cttcagggtc aggacacagg
     2521 ttgaacatca cggcggagaa cgactgccgg cggctgcact gctccctgag agacttgagc
     2581 tccctgctgc aggccgtggg ccgcctggcc gagtacttta tcggggatgt gtttgctgca
     2641 cggttcaatg atgccctcac agtcgtggaa aggttggtca aagtcactct gtacggatct
     2701 cagataaaat tgtacaacat tgaaactgct gtgccatcag tattgaaacc tgacctcatt
     2761 gatgtgcatg ctcagtccct ggctgcgctg caggcttact ctcactggtt agcacagtat
     2821 tgcagtgaag ttcaccggca gaacacgcag cagttcgtga cactcatctc tactaccatg
     2881 gatgcaatca cacctctaat cagcaccaag gtccaagaca agctgctgct atctgcgtgc
     2941 cacttactgg tctcactggc caccaccgtg cggcccgtct ttctgatcag catccctgca
     3001 gtgcagaaag tattcaacag aatcactgat gcctctgccc tgcgacttgt cgataaggcc
     3061 caggtgttgg tgtgccgagc cctctctaac atcttgctgc ttccgtggcc aaaccttcca
     3121 gagaatgagc agcagtggcc cgtgcgctcc atcaaccatg ccagcctcat ctctgcactc
     3181 tcccgggact atcgcaacct gaagcccagt gctgttgccc cacagagaaa gatgccactg
     3241 gatgacacca aactgattat ccaccagaca ctcagcgtct tagaagatat tgtggagaat
     3301 atctcggggg agtccaccaa gtctcgacag atttgctacc agtcgctgca ggaatctgtt
     3361 caggtctccc tggccctctt tccagctttt atccatcagt cagatgtgac tgatgagatg
     3421 ctgagcttct tcctcactct gtttcgaggc cttagagtac agatgggtgt gcctttcact
     3481 gagcaaatca tacagacttt cctcaacatg tttaccagag agcagttagc cgagagcatc
     3541 ctccacgagg gcagcacagg ctgccgggtg gtggagaagt ttctgaagat cctgcaggtg
     3601 gtggtccagg agccaggcca ggtgttcaag cccttcctcc ccagcatcat cgccctgtgc
     3661 atggagcaag tgtatcccat cattgccgag cgtccctccc ctgatgtgaa ggccgagctg
     3721 tttgagctcc ttttccggac gctccatcac aactggaggt acttcttcaa gtccaccgtg
     3781 ctggccagtg tccagagggg gatcgctgag gagcagatgg agaatgagcc ccagttcagt
     3841 gccatcatgc aggctttcgg acagtccttt ctccagcccg acatccacct ttttaaacaa
     3901 aatctcttct acttggagac tctcaacacc aagcagaagc tgtaccacaa gaagatcttc
     3961 cggactgcca tgctgttcca gtttgtgaac gtgctgctcc aggtcctggt ccacaagtcc
     4021 catgatcttc tgcaggagga gattggcatc gccatctaca acatggcctc agtcgacttt
     4081 gatggcttct ttgccgcctt cctcccagag ttcctgacca gctgtgatgg tgtggatgcc
     4141 aaccagaaaa gtgtgctggg gcggaatttc aagatggatc gggacctgcc ctcattcacc
     4201 cagaatgtgc acaggctggt caacgacctg cgctactaca gactctgcaa cgacagcctg
     4261 ccccctggca ctgtgaagct cgaattcgcc gccaccatgg cggccgccag ctggagccac
     4321 cctcagttcg agaagggagg aggaagcggc ggaggcagcg gaggaggaag ctggagccac
     4381 ccgcagttcg agaaatgact cgagcatgca tctagaggct ggagccaccc gcagttcgag
     4441 aaatgactcg agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagc
     4501 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     4561 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     4621 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4681 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4741 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     4801 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4861 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4921 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4981 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5041 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5101 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     5161 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     5221 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     5281 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     5341 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     5401 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5461 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     5521 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     5581 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     5641 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     5701 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     5761 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     5821 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     5881 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     5941 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     6001 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     6061 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     6121 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     6181 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     6241 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     6301 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     6361 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     6421 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     6481 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     6541 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     6601 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6661 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6721 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     6781 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6841 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6901 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6961 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     7021 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     7081 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     7141 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7201 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7261 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7321 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7381 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7441 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7501 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7561 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7621 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7681 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7741 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7801 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7861 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7921 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7981 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8041 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8101 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8161 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8221 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8281 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8341 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8401 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8461 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8521 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8581 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8641 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8701 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8761 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8821 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8881 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacgga
//
