LOCUS       sequence-417618-       10630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sox10 promoter driving HA-tagged biotin ligase (BirA), a Thosea 
            asigna virus peptide (2A), and membrane-tethered mCherry protein 
            (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 10630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-sox10-cytoBirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..10630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(106..125)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      919..1507
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1408..1427
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1661..1678
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1795..1816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1831..1836,1837..1854,1855..1861)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1869..1885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1874..1896
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1893..1909
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1893..1909
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1928..1948
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1930..1948
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1985..2001
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1985..2001
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(2035..2046,2047..2234)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     promoter        7153..7171
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        7251..7269
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      7331..7340
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7346..7372
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             7379..7405
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             7412..7438
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             7454..8416
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             7454..8416
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             8432..8485
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8492..9199
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8637..8655)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8905..8924
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(9316..9334)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    9341..9475
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9365..9384)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9419..9438
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(9567..9704,9705..9716)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9775..9792)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9775..9791)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9784..9806)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9933..10387
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10019..10038)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10229..10250
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10413..10517
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10518..10586,10587..10630,1..748)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
       61 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
      121 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
      181 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
      241 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
      301 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
      361 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
      421 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
      481 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
      541 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
      601 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
      661 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
      721 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
      781 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
      841 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
      901 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
      961 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1021 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     1081 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     1141 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     1201 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     1261 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     1321 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     1381 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     1441 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     1501 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     1561 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     1621 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     1681 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1741 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     1801 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     1861 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     1921 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctccacc gcggtggcgg
     1981 ccgctctaga actagtggat ctgctgggct tgctgaaggt agggggtcaa gaaccagagg
     2041 tgtaaagtac ttgagtaatt ttacttgatt actgtactta agtattattt ttggggattt
     2101 ttactttact tgagtacaat taaaaatcaa tacttttact tttacttaat tacatttttt
     2161 tagaaaaaaa agtacttttt actccttaca attttattta cagtcaaaaa gtacttattt
     2221 tttggagatc acttgggccc ggctcgagct acgtacttaa gcttgaggat cccatcgatt
     2281 cgaattcctg cagcccgggg gatcccctta tcagagtcaa cattcatgga taatgaaaaa
     2341 ggcaatgatt tttggcttgg gtgggggggc cgtcgtgaga tggaaataca agaggttatt
     2401 gttgaccaga acagcgcgcg tgtttgtgtg cgtttggccc gtcgtttgcc tctcttctcg
     2461 tctttctata ttttctacta aagctgttta aacgcgttaa atgcgctctc tgtgtgtgtt
     2521 tatgcgcgct gatgcaatta taacactaac acacatagcg cgacacgatg caggagttca
     2581 ttccagtgct gcgggcctgc gcgcgcagca tgaacacacc actgcgagca aatctgcaat
     2641 attaatgaat atatataact aaagtgtgca ctttctggtt tataactgca gcaccccgca
     2701 tacaccacag tataaagtaa tctacaatat taatgaatat aataaacaag aatgttcact
     2761 tttaggttta agaatgcagg cctgcgcgcg cagcatcccg cgagcacaac actagaaact
     2821 aaactccaat attagttaat atattcaatt aaaatgtaca ctttctggtt taaaactgca
     2881 agtatgcgtg cgcagcatcc gacataccca acactagaaa ctaatctaca atatttatga
     2941 aagtattcaa ctaaaatgtt cactttctgg tttaaaactg caagtatacg cgtgcgcagc
     3001 atccgacata cccaacacta gaaattaatc taaattatta attaaagtat tcaactgaat
     3061 tgttcacttt ctggtttaaa actgcaagta tgggtgcgca gcataaaaca tacccaacac
     3121 tagaaactaa tctacaatat taatggatgt attcaactaa aatgttcact ttctggttta
     3181 aaactgcagg aatgcgcgcg caacaccctg cggataactt taaaaacaat tccgcaatca
     3241 gctaaaactg aaaagttcag ttgattaatg aatgtaggca tgcgcaaaat attataaatg
     3301 cgcacacaca tctatttaat acattaaaac taaggctaaa tgttcacttt caccattttg
     3361 gggaaaatga aaattcagtg tcccccttaa gtggccagct ttagggcaat aggtgaccct
     3421 ccataagtag cataaaatcg taggctattc tgggcaccct ttgacatcgc cacgcgttaa
     3481 acagagaatg gagaggttat tgcgcacagc aggtgcgcag agtcgcgtcc gctaatggcg
     3541 ctggagctgc tgacaggtcc gctggcgctc tgcggggtgg aagcgctcga aaccccgcac
     3601 aacccctgat tcaccctgag gcacagccag gccaacaacg ggccagtgtg atcggaaaag
     3661 agttgaaaga atctccgtga caccaatgag gagccaaaga ccacctgtga aatacatcac
     3721 atgtcagaca aaagcatatt aagctgatca aatattgcgt gcataaccgc attacatcac
     3781 aaagaaaaac attagctagc tatgggtaac ttaataagtg ttctacattt gcactgcagc
     3841 catggttgtg agccgaaaag gtttgcgcaa taatattcac cccccgagat ttacattttt
     3901 tttttgcggt tctctgatgt cgattaatgg ccacgtggcc ttcaggtaaa cacatttaat
     3961 atttaactta tgtgttttaa ttagtgtgtt ccgaattatt cggttatttt tatttaacat
     4021 ttttattaat tagcttaatt ctgatccact cccaactctt attaacgaat cacaaatcaa
     4081 atggatagtt ttaattgcac aattagcctg tcaaaactta acttaaagct gttaatgcac
     4141 tgcttttaag tttgaaaaat aaagaaaaat tattagaaaa cttaagatta gattttaaca
     4201 tatgttgaaa ttatcaaatc ggcatgttta tgataaaaca actttaccta aaactaaaat
     4261 tcagatcaac aaaaacctaa atgaagttac atatatatat atatgtgtgt gtgtgtgtgt
     4321 atatatatat ataaatgtaa ggcaaaggag gattatttaa atagaatgtc atacacaatg
     4381 gaaaatcaaa gtgttttaca taaacaacaa taaaagaaac ataaataaca agcgtaaaag
     4441 caaattcaaa ctgaataata aaaaaataat aaagttgaag acacaaatgt atatttaaaa
     4501 aagtttaaga tctgaattaa aagtaagatg cccctgtgag aggccaaata ttactgtcgt
     4561 tgatttgcag ccgaaatcac cgccctctgg ggcaaaactc agcagccaat cagagcggga
     4621 cccgacaggg ctctgttgct cctttaagag atttttttcc atgaattgat ttttttctcg
     4681 ctcttcacac aacggggctc tttaagcctc gacgcgcgac acagagcagg cattcagagc
     4741 gcgagcgagg gggctgaacc gacggactct cgtgctgggc gggcgacctg ctgcactgta
     4801 aagtaagtgc attactggct ttctctctat cgcttactct gtgtgggaat atatacgcca
     4861 ttgcactgtc taacggagaa acttttacat ttcttattag taataatact actaataata
     4921 agcggtgcat ttataaacga catctactta ttttggattt ttcggtttat gtattgatgg
     4981 gtttggtcgt ggataattgt aatggagaag gtttttaatt gaaatggatt aaaggacgtc
     5041 tttaaaagtg caacttatct aatcttttgg cgaaaggaac acttaaagtt ggtgtcacgg
     5101 ggcataaatt tcgagatttg cgtgaatgtg taaatcagag acgtgttggg ttaatctgtc
     5161 ttaattggaa agaacggctt aatctatctg tgcacggacc acttgggatg tacatacact
     5221 tttagatctt ttagtaaatg tttacagtcc gataaattat acatttgaac actttaattc
     5281 gaagtgaatc agtttttaaa tagttttata agaaataatt tttttaaatt taatcttctg
     5341 agaagtttta cttgcgtaag gctatttgta tatatcgccg tctcttgcca aattgctcat
     5401 gtaaccaacg caaagattaa caaagagacc aaactttaaa gtacacaatt tataatattg
     5461 tttatctaca catcctttca caacaaatat aacaggatca gaaaacttaa gtaaagtaaa
     5521 actaactgaa atgcacattt gtgtaagttt gaatcaaatg tttgatacaa taataatctc
     5581 cgcagattag tttgaaaggt ttcattcgaa tgagtttgtg tcatttatga gttttgtgtt
     5641 ttggcatgag tgaattaggg gcaaattatt gccaaaccag gcgttatgat ccgccctaca
     5701 gcagccaaag atgaacccaa tcaaatctga acaaataaag tttacagcgg aggaaactca
     5761 aataagcaag agttgcgagc aaagttaaaa tgaatgagca taaagtttat aaaagtcctc
     5821 ctgacagaaa acagccgctg aaatcttttc cttaaaacaa aataaaccca gcagcgcaat
     5881 taccgaaatt gacggataca tttgttagct actaaaacaa accaagtctt gtgcgatgct
     5941 ttctttttcg tcccaaataa attggacaag tcaaaccatt aacgctttct gtaggctata
     6001 catgtgatta acaaatgatc aatgtgttaa tgtgttttgt aaggggtcta caagtagcca
     6061 ttcttggcta actctagcac attttacagt aatgtttatt aatgtgcatt gaccctgtaa
     6121 acaaataaac taaacatggt tctgctcatg tgaagtcacc tgtatcttaa ttaacactgc
     6181 tgttggatgc tgtgtttttg tattcgggtt aatattggat gtctgtaact caagttcaag
     6241 ctgacaggtc gcatctcaac agcttctgtc gcatataaat tatttgatcg atttttctgt
     6301 aattggacct agtcaaccac atccactcgt ttaattcacg attacttcac atttcagaaa
     6361 cacactgcca tgactctctt gatttttttc ttcttctgct ggttataagt cgtcgtttgt
     6421 gctttcacca acaaacactg tgtaattcag ctgcccacgg cagcgccgct ccgtgtttat
     6481 ccagcagcga ggcggaatga agccggtatt attccagcac tttctctccc gggagcttga
     6541 acaaaccgcc aaaccttcaa cagtagcttg ttagcagaag tgaagctaaa ataacacaca
     6601 cacatataca cacaccgagg cccactgacg ccaccgtatg attgcagcgc tgccctggaa
     6661 aaaggcgact tctaaataaa gtcaaagcct cagtgtttgt aggtttttcc tgaacaggtt
     6721 tatcttgttc atgaggtgtt cgggtgaaca gcaaaggggt tgtgcggtca tacagccaac
     6781 cattaaaacg acatattaat gctattttat acattaggaa gacatgcttt gcagaagcaa
     6841 gctgtttaat aaaataataa caatgattat tataactaat aaattacctg tgtgtgtatt
     6901 tttgtaggtt tccatcagat ctatatccag aggaagacgg cggaaggatt ccttcttaca
     6961 aactcgaatt atataaaaag aactgttaag gtttcactgg atgatcttaa ataataaaca
     7021 aaagcacaat tattttacaa gaaaaaaaca tttgtaaagt ataaattaat acatttatat
     7081 ttaaaataaa atttaagtga ggaaattaaa cctaccgaag tcacctgtgg ccgcagaact
     7141 agttctaggc ctatttaggt gacactatag aagagtacaa gctacttgtt ctttttgcag
     7201 gatcccatcg attcgaattc tcggtaccta gtcgaccttc gcgaaggcct atttaggtga
     7261 cactatagaa gagtacaatt caaggttaag cggccgcaag ctagattggc attccggtac
     7321 tgttggtaaa gccaccatgg ccacctatga cgtcccagat tacgcaagtt tgcccgggta
     7381 tgatgttcct gattatgcta gcctcccggg ttacgatgtg cccgactatg cctcacttcc
     7441 aggcagatct cctatgaagg ataacaccgt gccactgaaa ttgattgccc tgttagcgaa
     7501 cggtgaattt cactctggcg agcagttggg tgaaacgctg ggaatgagcc gggcggctat
     7561 taataaacac attcagacac tgcgtgactg gggcgttgat gtctttaccg ttccgggtaa
     7621 aggatacagc ctgcctgagc ctatccagtt acttaatgct aaacagatat tgggtcagct
     7681 ggatggcggt agtgtagccg tgctgccagt gattgactcc acgaatcagt accttcttga
     7741 tcgtatcgga gagcttaaat cgggcgatgc ttgcattgca gaataccagc aggctggccg
     7801 tggtcgccgg ggtcggaaat ggttttcgcc ttttggcgca aacttatatt tgtcgatgtt
     7861 ctggcgtctg gaacaaggcc cggcggcggc gattggttta agtctggtta tcggtatcgt
     7921 gatggcggaa gtattacgca agctgggtgc agataaagtt cgtgttaaat ggcctaatga
     7981 cctctatctg caggatcgca agctggcagg cattctggtg gagctgactg gcaaaactgg
     8041 cgatgcggcg caaatagtca ttggagccgg gatcaacatg gcaatgcgcc gtgttgaaga
     8101 gagtgtcgtt aatcaggggt ggatcacgct gcaggaagcg gggatcaatc tcgatcgtaa
     8161 tacgttggcg gccatgctaa tacgtgaatt acgtgctgcg ttggaactct tcgaacaaga
     8221 aggattggca ccttatctgt cgcgctggga aaagctggat aattttatta atcgcccagt
     8281 gaaacttatc attggtgata aagaaatatt tggcatttca cgcggaatag acaaacaggg
     8341 ggctttatta cttgagcagg atggaataat aaaaccctgg atgggcggtg aaatatccct
     8401 gcgtagtgca gaaaaagata tcggatcagg agagggcagg ggaagtcttc taacatgcgg
     8461 ggacgtggag gaaaatcccg ggcccagatc tatggtgagc aagggcgagg aggataacat
     8521 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     8581 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     8641 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     8701 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     8761 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     8821 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     8881 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     8941 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     9001 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     9061 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     9121 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     9181 catggacgag ctgtacaagg gaggaggaag atctaagctg aaccctcctg atgagagtgg
     9241 ccccggctgc atgagctgca agtgtgtgct ctcctgatct agaactatag tgagtcgtat
     9301 tacgtatccg gactccctat agtgagtcgt attaaccggt gatccagaca tgataagata
     9361 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     9421 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     9481 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttgata
     9541 tcccgggttt aaacgcatgc agatctaata ctcaagtaca attttaatgg agtacttttt
     9601 tacttttact caagtaagat tctagccaga tacttttact tttaattgag taaaattttc
     9661 cctaagtact tgtactttca cttgagtaaa atttttgagt actttttaca cctctgtcaa
     9721 gaaccatatg ccggtaccca attcgcccta taggacgtcg tattacgcgc gctcactggc
     9781 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     9841 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     9901 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     9961 ggcgggtgtg gtggttacgc gcagcgtgac caccacaccc gccgcgccct agcgcccgct
    10021 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    10081 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    10141 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    10201 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    10261 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    10321 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    10381 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    10441 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    10501 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10561 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10621 aagatcagtt
//
