LOCUS       sequence-417621-       10657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a 
            Thosea asigna virus peptide (2A), and membrane-tethered mCherry 
            protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 10657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMT-sox10-NLS-BirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..10657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4383..4401
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        4481..4499
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      4561..4570
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4576..4602
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4609..4635
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4642..4668
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4684..4704
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4711..5673
                     /codon_start=1
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             4711..5673
                     /codon_start=1
                     /gene="birA"
                     /product="E. coli biotin protein ligase"
                     /label=BirA
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             5689..5742
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5749..6456
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5894..5912)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6162..6181
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(6573..6591)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    6598..6732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6622..6641)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6676..6695
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(6824..6961,6962..6973)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7032..7049)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7032..7048)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7041..7063)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7190..7644
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7276..7295)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7486..7507
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7670..7774
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7775..7843,7844..8635)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7993..8012)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8806..9394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9295..9314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9548..9565
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9682..9703
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9718..9723,9724..9741,9742..9748)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9756..9772
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9761..9783
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9780..9796
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9780..9796
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9815..9835
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9817..9835
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     9872..9888
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9872..9888
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(9922..9933,9934..10121)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 agaatgcagg cctgcgcgcg cagcatcccg cgagcacaac actagaaact aaactccaat
       61 attagttaat atattcaatt aaaatgtaca ctttctggtt taaaactgca agtatgcgtg
      121 cgcagcatcc gacataccca acactagaaa ctaatctaca atatttatga aagtattcaa
      181 ctaaaatgtt cactttctgg tttaaaactg caagtatacg cgtgcgcagc atccgacata
      241 cccaacacta gaaattaatc taaattatta attaaagtat tcaactgaat tgttcacttt
      301 ctggtttaaa actgcaagta tgggtgcgca gcataaaaca tacccaacac tagaaactaa
      361 tctacaatat taatggatgt attcaactaa aatgttcact ttctggttta aaactgcagg
      421 aatgcgcgcg caacaccctg cggataactt taaaaacaat tccgcaatca gctaaaactg
      481 aaaagttcag ttgattaatg aatgtaggca tgcgcaaaat attataaatg cgcacacaca
      541 tctatttaat acattaaaac taaggctaaa tgttcacttt caccattttg gggaaaatga
      601 aaattcagtg tcccccttaa gtggccagct ttagggcaat aggtgaccct ccataagtag
      661 cataaaatcg taggctattc tgggcaccct ttgacatcgc cacgcgttaa acagagaatg
      721 gagaggttat tgcgcacagc aggtgcgcag agtcgcgtcc gctaatggcg ctggagctgc
      781 tgacaggtcc gctggcgctc tgcggggtgg aagcgctcga aaccccgcac aacccctgat
      841 tcaccctgag gcacagccag gccaacaacg ggccagtgtg atcggaaaag agttgaaaga
      901 atctccgtga caccaatgag gagccaaaga ccacctgtga aatacatcac atgtcagaca
      961 aaagcatatt aagctgatca aatattgcgt gcataaccgc attacatcac aaagaaaaac
     1021 attagctagc tatgggtaac ttaataagtg ttctacattt gcactgcagc catggttgtg
     1081 agccgaaaag gtttgcgcaa taatattcac cccccgagat ttacattttt tttttgcggt
     1141 tctctgatgt cgattaatgg ccacgtggcc ttcaggtaaa cacatttaat atttaactta
     1201 tgtgttttaa ttagtgtgtt ccgaattatt cggttatttt tatttaacat ttttattaat
     1261 tagcttaatt ctgatccact cccaactctt attaacgaat cacaaatcaa atggatagtt
     1321 ttaattgcac aattagcctg tcaaaactta acttaaagct gttaatgcac tgcttttaag
     1381 tttgaaaaat aaagaaaaat tattagaaaa cttaagatta gattttaaca tatgttgaaa
     1441 ttatcaaatc ggcatgttta tgataaaaca actttaccta aaactaaaat tcagatcaac
     1501 aaaaacctaa atgaagttac atatatatat atatgtgtgt gtgtgtgtgt atatatatat
     1561 ataaatgtaa ggcaaaggag gattatttaa atagaatgtc atacacaatg gaaaatcaaa
     1621 gtgttttaca taaacaacaa taaaagaaac ataaataaca agcgtaaaag caaattcaaa
     1681 ctgaataata aaaaaataat aaagttgaag acacaaatgt atatttaaaa aagtttaaga
     1741 tctgaattaa aagtaagatg cccctgtgag aggccaaata ttactgtcgt tgatttgcag
     1801 ccgaaatcac cgccctctgg ggcaaaactc agcagccaat cagagcggga cccgacaggg
     1861 ctctgttgct cctttaagag atttttttcc atgaattgat ttttttctcg ctcttcacac
     1921 aacggggctc tttaagcctc gacgcgcgac acagagcagg cattcagagc gcgagcgagg
     1981 gggctgaacc gacggactct cgtgctgggc gggcgacctg ctgcactgta aagtaagtgc
     2041 attactggct ttctctctat cgcttactct gtgtgggaat atatacgcca ttgcactgtc
     2101 taacggagaa acttttacat ttcttattag taataatact actaataata agcggtgcat
     2161 ttataaacga catctactta ttttggattt ttcggtttat gtattgatgg gtttggtcgt
     2221 ggataattgt aatggagaag gtttttaatt gaaatggatt aaaggacgtc tttaaaagtg
     2281 caacttatct aatcttttgg cgaaaggaac acttaaagtt ggtgtcacgg ggcataaatt
     2341 tcgagatttg cgtgaatgtg taaatcagag acgtgttggg ttaatctgtc ttaattggaa
     2401 agaacggctt aatctatctg tgcacggacc acttgggatg tacatacact tttagatctt
     2461 ttagtaaatg tttacagtcc gataaattat acatttgaac actttaattc gaagtgaatc
     2521 agtttttaaa tagttttata agaaataatt tttttaaatt taatcttctg agaagtttta
     2581 cttgcgtaag gctatttgta tatatcgccg tctcttgcca aattgctcat gtaaccaacg
     2641 caaagattaa caaagagacc aaactttaaa gtacacaatt tataatattg tttatctaca
     2701 catcctttca caacaaatat aacaggatca gaaaacttaa gtaaagtaaa actaactgaa
     2761 atgcacattt gtgtaagttt gaatcaaatg tttgatacaa taataatctc cgcagattag
     2821 tttgaaaggt ttcattcgaa tgagtttgtg tcatttatga gttttgtgtt ttggcatgag
     2881 tgaattaggg gcaaattatt gccaaaccag gcgttatgat ccgccctaca gcagccaaag
     2941 atgaacccaa tcaaatctga acaaataaag tttacagcgg aggaaactca aataagcaag
     3001 agttgcgagc aaagttaaaa tgaatgagca taaagtttat aaaagtcctc ctgacagaaa
     3061 acagccgctg aaatcttttc cttaaaacaa aataaaccca gcagcgcaat taccgaaatt
     3121 gacggataca tttgttagct actaaaacaa accaagtctt gtgcgatgct ttctttttcg
     3181 tcccaaataa attggacaag tcaaaccatt aacgctttct gtaggctata catgtgatta
     3241 acaaatgatc aatgtgttaa tgtgttttgt aaggggtcta caagtagcca ttcttggcta
     3301 actctagcac attttacagt aatgtttatt aatgtgcatt gaccctgtaa acaaataaac
     3361 taaacatggt tctgctcatg tgaagtcacc tgtatcttaa ttaacactgc tgttggatgc
     3421 tgtgtttttg tattcgggtt aatattggat gtctgtaact caagttcaag ctgacaggtc
     3481 gcatctcaac agcttctgtc gcatataaat tatttgatcg atttttctgt aattggacct
     3541 agtcaaccac atccactcgt ttaattcacg attacttcac atttcagaaa cacactgcca
     3601 tgactctctt gatttttttc ttcttctgct ggttataagt cgtcgtttgt gctttcacca
     3661 acaaacactg tgtaattcag ctgcccacgg cagcgccgct ccgtgtttat ccagcagcga
     3721 ggcggaatga agccggtatt attccagcac tttctctccc gggagcttga acaaaccgcc
     3781 aaaccttcaa cagtagcttg ttagcagaag tgaagctaaa ataacacaca cacatataca
     3841 cacaccgagg cccactgacg ccaccgtatg attgcagcgc tgccctggaa aaaggcgact
     3901 tctaaataaa gtcaaagcct cagtgtttgt aggtttttcc tgaacaggtt tatcttgttc
     3961 atgaggtgtt cgggtgaaca gcaaaggggt tgtgcggtca tacagccaac cattaaaacg
     4021 acatattaat gctattttat acattaggaa gacatgcttt gcagaagcaa gctgtttaat
     4081 aaaataataa caatgattat tataactaat aaattacctg tgtgtgtatt tttgtaggtt
     4141 tccatcagat ctatatccag aggaagacgg cggaaggatt ccttcttaca aactcgaatt
     4201 atataaaaag aactgttaag gtttcactgg atgatcttaa ataataaaca aaagcacaat
     4261 tattttacaa gaaaaaaaca tttgtaaagt ataaattaat acatttatat ttaaaataaa
     4321 atttaagtga ggaaattaaa cctaccgaag tcacctgtgg ccgcagaact agttctaggc
     4381 ctatttaggt gacactatag aagagtacaa gctacttgtt ctttttgcag gatcccatcg
     4441 attcgaattc tcggtaccta gtcgaccttc gcgaaggcct atttaggtga cactatagaa
     4501 gagtacaatt caaggttaag cggccgcaag ctagattggc attccggtac tgttggtaaa
     4561 gccaccatgg ccacctatga cgtcccagat tacgcaagtt tgcccgggta tgatgttcct
     4621 gattatgcta gcctcccggg ttacgatgtg cccgactatg cctcacttcc aggcagatct
     4681 cctccaaaaa agaagagaaa ggtacgatct atgaaggata acaccgtgcc actgaaattg
     4741 attgccctgt tagcgaacgg tgaatttcac tctggcgagc agttgggtga aacgctggga
     4801 atgagccggg cggctattaa taaacacatt cagacactgc gtgactgggg cgttgatgtc
     4861 tttaccgttc cgggtaaagg atacagcctg cctgagccta tccagttact taatgctaaa
     4921 cagatattgg gtcagctgga tggcggtagt gtagccgtgc tgccagtgat tgactccacg
     4981 aatcagtacc ttcttgatcg tatcggagag cttaaatcgg gcgatgcttg cattgcagaa
     5041 taccagcagg ctggccgtgg tcgccggggt cggaaatggt tttcgccttt tggcgcaaac
     5101 ttatatttgt cgatgttctg gcgtctggaa caaggcccgg cggcggcgat tggtttaagt
     5161 ctggttatcg gtatcgtgat ggcggaagta ttacgcaagc tgggtgcaga taaagttcgt
     5221 gttaaatggc ctaatgacct ctatctgcag gatcgcaagc tggcaggcat tctggtggag
     5281 ctgactggca aaactggcga tgcggcgcaa atagtcattg gagccgggat caacatggca
     5341 atgcgccgtg ttgaagagag tgtcgttaat caggggtgga tcacgctgca ggaagcgggg
     5401 atcaatctcg atcgtaatac gttggcggcc atgctaatac gtgaattacg tgctgcgttg
     5461 gaactcttcg aacaagaagg attggcacct tatctgtcgc gctgggaaaa gctggataat
     5521 tttattaatc gcccagtgaa acttatcatt ggtgataaag aaatatttgg catttcacgc
     5581 ggaatagaca aacagggggc tttattactt gagcaggatg gaataataaa accctggatg
     5641 ggcggtgaaa tatccctgcg tagtgcagaa aaagatatcg gatcaggaga gggcagggga
     5701 agtcttctaa catgcgggga cgtggaggaa aatcccgggc ccagatctat ggtgagcaag
     5761 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     5821 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     5881 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     5941 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     6001 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     6061 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     6121 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     6181 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     6241 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     6301 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     6361 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     6421 cgccactcca ccggcggcat ggacgagctg tacaagggag gaggaagatc taagctgaac
     6481 cctcctgatg agagtggccc cggctgcatg agctgcaagt gtgtgctctc ctgatctaga
     6541 actatagtga gtcgtattac gtatccggac tccctatagt gagtcgtatt aaccggtgat
     6601 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     6661 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     6721 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     6781 tgggaggttt tttgatatcc cgggtttaaa cgcatgcaga tctaatactc aagtacaatt
     6841 ttaatggagt acttttttac ttttactcaa gtaagattct agccagatac ttttactttt
     6901 aattgagtaa aattttccct aagtacttgt actttcactt gagtaaaatt tttgagtact
     6961 ttttacacct ctgtcaagaa ccatatgccg gtacccaatt cgccctatag gacgtcgtat
     7021 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     7081 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     7141 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     7201 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccac cacacccgcc
     7261 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     7321 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7381 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7441 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7501 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7561 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7621 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7681 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7741 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7801 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7861 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7921 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7981 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     8041 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8101 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8161 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8221 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8281 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8341 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8401 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8461 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8521 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8581 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8641 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8701 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8761 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8821 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8881 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8941 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     9001 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9061 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9181 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9421 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9481 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9541 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9601 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     9661 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     9721 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     9781 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     9841 aaagctggag ctccaccgcg gtggcggccg ctctagaact agtggatctg ctgggcttgc
     9901 tgaaggtagg gggtcaagaa ccagaggtgt aaagtacttg agtaatttta cttgattact
     9961 gtacttaagt attatttttg gggattttta ctttacttga gtacaattaa aaatcaatac
    10021 ttttactttt acttaattac atttttttag aaaaaaaagt actttttact ccttacaatt
    10081 ttatttacag tcaaaaagta cttatttttt ggagatcact tgggcccggc tcgagctacg
    10141 tacttaagct tgaggatccc atcgattcga attcctgcag cccgggggat ccccttatca
    10201 gagtcaacat tcatggataa tgaaaaaggc aatgattttt ggcttgggtg ggggggccgt
    10261 cgtgagatgg aaatacaaga ggttattgtt gaccagaaca gcgcgcgtgt ttgtgtgcgt
    10321 ttggcccgtc gtttgcctct cttctcgtct ttctatattt tctactaaag ctgtttaaac
    10381 gcgttaaatg cgctctctgt gtgtgtttat gcgcgctgat gcaattataa cactaacaca
    10441 catagcgcga cacgatgcag gagttcattc cagtgctgcg ggcctgcgcg cgcagcatga
    10501 acacaccact gcgagcaaat ctgcaatatt aatgaatata tataactaaa gtgtgcactt
    10561 tctggtttat aactgcagca ccccgcatac accacagtat aaagtaatct acaatattaa
    10621 tgaatataat aaacaagaat gttcactttt aggttta
//
