LOCUS       sequence-417625-        9764 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Packaging plasmid for v3 PE-eVLP production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9764)
  AUTHORS   An M, Raguram A, Du SW, Banskota S, Davis JR, Newby GA, Chen PZ, 
            Palczewski K, Liu DR
  TITLE     Engineered virus-like particles for transient delivery of prime 
            editor ribonucleoprotein complexes in vivo.
  JOURNAL   Nat Biotechnol. 2024 Jan 8. doi: 10.1038/s41587-023-02078-y.
  PUBMED    38191664
REFERENCE   2  (bases 1 to 9764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 8. doi: 10.1038/s41587-023-02078-y."
COMMENT     Sequence Label: gag-MCP-pol
FEATURES             Location/Qualifiers
     source          1..9764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        96..475
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        476..679
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     630..650
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     676..700
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             837..1253
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1183..1205
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1221..1237
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             2481..2867
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     misc_feature    6051..6425
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     6363..6379
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(6560..6577)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6566..6790
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6864..6883)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6865..6883)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6890..6907)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6890..6906)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6899..6921)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7048..7503
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7135..7154)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7345..7366
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7529..7633
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7634..7702,7703..8494)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7852..7871)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8665..9253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9154..9173
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9407..9424
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9541..9562
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9577..9582,9583..9600,9601..9607)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9615..9631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9620..9642
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9639..9655
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9639..9655
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9676..9694
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ataaatcaat attggctatt ggccattgca tacgttgtat ccatatcata atatgtacat
       61 ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta
      121 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      181 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      241 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      301 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      361 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      421 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      481 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      541 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      601 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      661 ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg
      721 ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccaagcttgg
      781 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aatatgggcc
      841 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
      901 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
      961 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1021 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1081 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1141 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1201 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccaaaccta
     1261 aacctcaagt tctttctgac agtggggggc cgctcatcga cctacttaca gaagaccccc
     1321 cgccttatag ggacccaaga ccaccccctt ccgacaggga cggaaatggt ggagaagcga
     1381 cccctgcggg agaggcaccg gacccctccc caatggcatc tcgcctacgt gggagacggg
     1441 agccccctgt ggccgactcc actacctcgc aggcattccc cctccgcgca ggaggaaacg
     1501 gacagcttca atactggccg ttctcctctt ctgaccttta caactggaaa aataataacc
     1561 cttctttttc tgaagatcca ggtaaactga cagctctgat cgagtctgtc ctcatcaccc
     1621 atcagcccac ctgggacgac tgtcagcagc tgttggggac tctgctgacc ggagaagaaa
     1681 aacaacgggt gctcttagag gctagaaagg cggtgcgggg cgatgatggg cgccccactc
     1741 aactgcccaa tgaagtcgat gccgcttttc ccctcgagcg cccagactgg gattacacca
     1801 cccaggcagg taggaaccac ctagtccact atcgccagtt gctcctagcg ggtctccaaa
     1861 acgcgggcag aagccccacc aatttggcca aggtaaaagg aataacacaa gggcccaatg
     1921 agtctccctc ggccttccta gagagactta aggaagccta tcgcaggtac actccttatg
     1981 accctgagga cccagggcaa gaaactaatg tgtctatgtc tttcatttgg cagtctgccc
     2041 cagacattgg gagaaagtta gagaggttag aagatttaaa aaacaagacg cttggagatt
     2101 tggttagaga ggcagaaaag atctttaata aacgagaaac cccggaagaa agagaggaac
     2161 gtatcaggag agaaacagag gaaaaagaag aacgccgtag gacagaggat gagcagaaag
     2221 agaaagaaag agatcgtagg agacatagag agatgagcaa gctattggcc actgtcgtta
     2281 gtggacagaa acaggataga cagggaggag aacgaaggag gtcccaactc gatcgcgacc
     2341 agtgtgccta ctgcaaagaa aaggggcact gggctaaaga ttgtcccaag aaaccacgag
     2401 gacctcgggg accaagaccc cagacctccc tcctgaccct agatgactcc ggtggaggtg
     2461 gatccggtgg aggttccatg gcctctaatt ttactcaatt tgtgcttgtc gataatgggg
     2521 ggacgggaga tgtgaccgtt gcccctagca atttcgcaaa tggcgttgca gaatggatct
     2581 ctagcaacag cagaagccaa gcgtacaaag taacgtgttc cgttcgccaa agctccgccc
     2641 aaaaacggaa gtatacaata aaggttgagg tgccgaaagt agccactcaa acagttggtg
     2701 gggtagaatt gcccgtagcg gcatggcggt catatctcaa tatggaactc actatcccaa
     2761 tcttcgccac gaatagcgat tgtgagctga tagttaaggc tatgcaaggt cttctcaaag
     2821 atggaaaccc tattccatct gctatcgccg ccaacagcgg gatatactcc ggaggtggag
     2881 gtggataggg aggtcagggt caggagcccc cccctgaacc caggataacc ctcaaagtcg
     2941 gggggcaacc cgtcaccttc ctggtagata ctggggccca acactccgtg ctgacccaaa
     3001 atcctggacc cctaagtgat aagtctgcct gggtccaagg ggctactgga ggaaagcggt
     3061 atcgctggac cacggatcgc aaagtacatc tagctaccgg taaggtcacc cactctttcc
     3121 tccatgtacc agactgtccc tatcctctgt taggaagaga tttgctgact aaactaaaag
     3181 cccaaatcca ctttgaggga tcaggagctc aggttatggg accaatgggg cagcccctgc
     3241 aagtgttgac cctaaatata gaagatgagt atcggctaca tgagacctca aaagagccag
     3301 atgtttctct agggtccaca tggctgtctg attttcctca ggcctgggcg gaaaccgggg
     3361 gcatgggact ggcagttcgc caagctcctc tgatcatacc tctgaaagca acctctaccc
     3421 ccgtgtccat aaaacaatac cccatgtcac aagaagccag actggggatc aagccccaca
     3481 tacagagact gttggaccag ggaatactgg taccctgcca gtccccctgg aacacgcccc
     3541 tgctacccgt taagaaacca gggactaatg attataggcc tgtccaggat ctgagagaag
     3601 tcaacaagcg ggtggaagac atccacccca ccgtgcccaa cccttacaac ctcttgagcg
     3661 ggctcccacc gtcccaccag tggtacactg tgcttgattt aaaggatgcc tttttctgcc
     3721 tgagactcca ccccaccagt cagcctctct tcgcctttga gtggagagat ccagagatgg
     3781 gaatctcagg acaattgacc tggaccagac tcccacaggg tttcaaaaac agtcccaccc
     3841 tgtttgatga ggcactgcac agagacctag cagacttccg gatccagcac ccagacttga
     3901 tcctgctaca gtacgtggat gacttactgc tggccgccac ttctgagcta gactgccaac
     3961 aaggtactcg ggccctgtta caaaccctag ggaacctcgg gtatcgggcc tcggccaaga
     4021 aagcccaaat ttgccagaaa caggtcaagt atctggggta tcttctaaaa gagggtcaga
     4081 gatggctgac tgaggccaga aaagagactg tgatggggca gcctactccg aagacccctc
     4141 gacaactaag ggagttccta gggacggcag gcttctgtcg cctctggatc cctgggtttg
     4201 cagaaatggc agcccccttg taccctctca ccaaaacggg gactctgttt aattggggcc
     4261 cagaccaaca aaaggcctat caagaaatca agcaagctct tctaactgcc ccagccctgg
     4321 ggttgccaga tttgactaag ccctttgaac tctttgtcga cgagaagcag ggctacgcca
     4381 aaggtgtcct aacgcaaaaa ctgggacctt ggcgtcggcc ggtggcctac ctgtccaaaa
     4441 agctagaccc agtagcagct gggtggcccc cttgcctacg gatggtagca gccattgccg
     4501 tactgacaaa ggatgcaggc aagctaacca tgggacagcc actagtcatt ctggcccccc
     4561 atgcagtaga ggcactagtc aaacaacccc ccgaccgctg gctttccaac gcccggatga
     4621 ctcactatca ggccttgctt ttggacacgg accgggtcca gttcggaccg gtggtagccc
     4681 tgaacccggc tacgctgctc ccactgcctg aggaagggct gcaacacaac tgccttgata
     4741 tcctggccga agcccacgga acccgacccg acctaacgga ccagccgctc ccagacgccg
     4801 accacacctg gtacacggat ggaagcagtc tcttacaaga gggacagcgt aaggcgggag
     4861 ctgcggtgac caccgagacc gaggtaatct gggctaaagc cctgccagcc gggacatccg
     4921 ctcagcgggc tgaactgata gcactcaccc aggccctaaa gatggcagaa ggtaagaagc
     4981 taaatgttta tactgatagc cgttatgctt ttgctactgc ccatatccat ggagaaatat
     5041 acagaaggcg tgggttgctc acatcagaag gcaaagagat caaaaataaa gacgagatct
     5101 tggccctact aaaagccctc tttctgccca aaagacttag cataatccat tgtccaggac
     5161 atcaaaaggg acacagcgcc gaggctagag gcaaccggat ggctgaccaa gcggcccgaa
     5221 aggcagccat cacagagact ccagacacct ctaccctcct catagaaaat tcatcaccct
     5281 acacctcaga acattttcat tacacagtga ctgatataaa ggacctaacc aagttggggg
     5341 ccatttatga taaaacaaag aagtattggg tctaccaagg aaaacctgtg atgcctgacc
     5401 agtttacttt tgaattatta gactttcttc atcagctgac tcacctcagc ttctcaaaaa
     5461 tgaaggctct cctagagaga agccacagtc cctactacat gctgaaccgg gatcgaacac
     5521 tcaaaaatat cactgagacc tgcaaagctt gtgcacaagt caacgccagc aagtctgccg
     5581 ttaaacaggg aactagggtc cgcgggcatc ggcccggcac tcattgggag atcgatttca
     5641 ccgagataaa gcccggattg tatggctata aatatcttct agtttttata gatacctttt
     5701 ctggctggat agaagccttc ccaaccaaga aagaaaccgc caaggtcgta accaagaagc
     5761 tactagagga gatcttcccc aggttcggca tgcctcaggt attgggaact gacaatgggc
     5821 ctgccttcgt ctccaaggtg agtcagacag tggccgatct gttggggatt gattggaaat
     5881 tacattgtgc atacagaccc caaagctcag gccaggtaga aagaatgaat agaaccatca
     5941 aggagacttt aactaaatta acgcttgcaa ctggctctag agactgggtg ctcctactcc
     6001 ccttagccct gtaccgagcc cgcaacacgc cgggccccca tggcctcacc ccatatgaga
     6061 tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta
     6121 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct
     6181 ggagacctct ggcggcagcc taccaagaac aactggaccg accggtggta cctcaccctt
     6241 accgagtcgg cgacacagtg tgggtccgcc gacaccagac taagaaccta gaacctcgct
     6301 ggaaaggacc ttacacagtc ctgctgacca cccccaccgc cctcaaagta gacggcatcg
     6361 cagcttggat acacgccgcc cacgtgaagg ctgccgaccc cgggggtgga ccatcctcta
     6421 gactgacatg gcgcgttcaa cgctctcaaa accccttaaa aataaggttg acgcgtcaac
     6481 ccgcgaggcc ccctaatccc cttaattctt ctgatgctca gaggggtcag tatcgaattc
     6541 ctgcagcccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6601 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6661 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6721 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6781 ggctctatgg cttctgaggc ggaaagaacc agctggggct cgatcctcta gagcggccgc
     6841 caccgcggtg gagctccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
     6901 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     6961 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     7021 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     7081 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     7141 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7201 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7261 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     7321 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7381 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7441 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     7501 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7561 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7621 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7681 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7741 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7801 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     7861 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     7921 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     7981 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8041 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8101 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8161 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8221 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8281 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8341 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8401 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8461 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8521 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8581 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8641 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8701 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8761 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     8821 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     8881 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     8941 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9001 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9061 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9121 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9181 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9241 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9301 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9361 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     9421 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9481 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     9541 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     9601 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     9661 ttacgccaag ctcgaaatta accctcacta aagggaacaa aagctggtac cgggcccccc
     9721 ctcgagatcc ggccattagc catattattc attggttata tagc
//
