LOCUS       sequence-417655-        9579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Spycatcher003 on the cell surface using mouse CD80 (short)
            hinge, transmembrane, and cytoplasmic domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Patel A, Andre V, Eguiguren SB, Barton MI, Burton J, Denham EM, 
            Pettmann J, Morch AM, Kutuzov MA, Siller-Farfan JA, Dustin ML, van 
            der Merwe PA, Dushek O
  TITLE     Using CombiCells, a platform for titration and combinatorial display
            of cell surface ligands, to study T-cell antigen sensitivity 
            modulation by accessory receptors.
  JOURNAL   EMBO J. 2024 Jan;43(1):132-150. doi: 10.1038/s44318-023-00012-1. 
            Epub 2023 Dec 18.
  PUBMED    38177315
REFERENCE   2  (bases 1 to 9579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-023-00012-1"; journalName: "EMBO J"; date: 
            "2024-01"; volume: "43"; issue: "1"; pages: "132-150"
COMMENT     Sequence Label: pHR-SIN-BX SpyCatcher003-(short)mCD80
FEATURES             Location/Qualifiers
     source          1..9579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    114..248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(151..170)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     205..224
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             416..1049
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1096..1221
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1718..1951
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2136..2180
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2465..2582
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2735..3142
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3162..3171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     3168..3227
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             3231..3569
                     /codon_start=1
                     /product="improved SpyCatcher variant that reacts with 
                     SpyTag003 at a rate approaching the diffusion limit (Keeble
                     et al., 2019)"
                     /label=SpyCatcher003
                     /translation="VTTLSGLSGEQGPSGDMTTEEDSATHIKFSKRDEDGRELAGATME
                     LRDSSGKTISTWISDGHVKDFYLYPGKYTFVETAAPDGYEVATPIEFTVNEDGQVTVDG
                     EATEGDAHT"
     misc_feature    3854..4442
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3907..3927)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4325..4336)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4445..4461)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4446..4462)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4556..4789
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4814..4832)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4815..4832)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5122..5141)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5355..5374)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5474..5496
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5534..5552)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5620..5724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5725..5793,5794..6585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5943..5962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6756..7344
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7245..7264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7498..7515
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7585..7605)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7590..7919
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7770..7905
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9053..9118
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9248..9268
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
       61 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
      121 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
      181 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
      241 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
      301 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
      361 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa
      421 gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa
      481 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
      541 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
      601 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
      661 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
      721 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
      781 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
      841 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
      901 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      961 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1021 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     1081 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     1141 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     1201 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     1261 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     1321 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     1381 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     1441 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     1501 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     1561 gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1621 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1681 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1741 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1801 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1861 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1921 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1981 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     2041 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     2101 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     2161 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     2221 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     2281 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     2341 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     2401 acagatccat tcgattagtg aacggatctc gacggtatcg ccaaatggca gtattcatcc
     2461 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     2521 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     2581 ttcgggttta ttacagggac agcagagatc cagtttggat cgataagctt gatatcgaat
     2641 tcctgcagcc ccgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa
     2701 agaccccacc tgtaggtttg gcaagctagc tgcagtaacg ccattttgca aggcatggaa
     2761 aaataccaaa ccaagaatag agaagttcag atcaagggcg ggtacatgaa aatagctaac
     2821 gttgggccaa acaggatatc tgcggtgagc agtttcggcc ccggcccggg gccaagaaca
     2881 gatggtcacc gcagtttcgg ccccggcccg aggccaagaa cagatggtcc ccagatatgg
     2941 cccaaccctc agcagtttct taagacccat cagatgtttc caggctcccc caaggacctg
     3001 aaatgaccct gcgccttatt tgaattaacc aatcagcctg cttctcgctt ctgttcgcgc
     3061 gcttctgctt cccgagctct ataaaagagc tcacaacccc tcactcggcg cgccagtcct
     3121 ccgacagact gagtcgcccg ggggggatcc cggtactcga ggccaccatg gagacagaca
     3181 cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac gtgaccacac
     3241 tgtccggact gtctggagag cagggaccat ccggcgacat gaccacagag gaggattctg
     3301 ccacacacat caagttcagc aagagggacg aggacggaag agagctggca ggagcaacca
     3361 tggagctgag ggatagctcc ggcaagacca tcagcacatg gatctccgac ggccacgtga
     3421 aggatttcta cctgtatccc ggcaagtaca cctttgtgga gacagcagca ccagacggat
     3481 atgaggtggc aacccctatc gagtttacag tgaacgagga cggacaggtg accgtggatg
     3541 gagaggcaac agagggcgat gcacacaccg gatctagcgg atctggtgga agccctcctg
     3601 atagcaagaa cacacttgtg ctctttgggg caggattcgg cgcagtaata acagtcgtcg
     3661 tcatcgttgt catcatcaaa tgcttctgta agcacagaag ctgtttcaga agaaatgagg
     3721 caagcagaga aacaaacaac agccttacct tcgggcctga agaagcatta gctgaacaga
     3781 ccgtcttcct ttaggcggcc gcgactctag agtcgacctg caggcatgca agcttgatat
     3841 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3901 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     3961 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4021 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4081 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4141 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4201 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     4261 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4321 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4381 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     4441 gcatcgatac cgtcgacctc gatcgaggga attaattcga gctcggtacc tttaagacca
     4501 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     4561 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     4621 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     4681 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     4741 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag catctagaat
     4801 taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat aaggttatgt
     4861 attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta gcatctggct
     4921 tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt ttggttggac
     4981 gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc gaaccaatca
     5041 gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc atcaccggcg
     5101 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
     5161 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
     5221 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
     5281 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa
     5341 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     5401 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     5461 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     5521 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5581 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5641 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5701 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5761 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5821 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5881 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5941 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6001 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6061 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6121 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     6181 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     6241 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     6301 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     6361 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     6421 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6481 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6541 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6601 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6661 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6721 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6781 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6841 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6901 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6961 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7021 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7081 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7141 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7201 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7261 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7321 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7381 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7441 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7501 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     7561 tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     7621 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     7681 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7741 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7801 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7861 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     7921 agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca
     7981 gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca
     8041 gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa
     8101 caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc
     8161 catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc
     8221 gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg
     8281 tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga
     8341 aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg
     8401 gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa
     8461 aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga
     8521 ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt
     8581 ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt
     8641 ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc
     8701 aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca
     8761 caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt
     8821 aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca
     8881 cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc
     8941 taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt
     9001 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     9061 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     9121 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     9181 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     9241 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     9301 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     9361 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
     9421 tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
     9481 tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
     9541 taaaggggtt aataaggaat atttgatgta tagtgcctt
//
