LOCUS       sequence-417656-        9480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Spycatcher003 on the cell surface using human CD52 hinge.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9480)
  AUTHORS   Patel A, Andre V, Eguiguren SB, Barton MI, Burton J, Denham EM, 
            Pettmann J, Morch AM, Kutuzov MA, Siller-Farfan JA, Dustin ML, van 
            der Merwe PA, Dushek O
  TITLE     Using CombiCells, a platform for titration and combinatorial display
            of cell surface ligands, to study T-cell antigen sensitivity 
            modulation by accessory receptors.
  JOURNAL   EMBO J. 2024 Jan;43(1):132-150. doi: 10.1038/s44318-023-00012-1. 
            Epub 2023 Dec 18.
  PUBMED    38177315
REFERENCE   2  (bases 1 to 9480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s44318-023-00012-1"; journalName: "EMBO J"; date: 
            "2024-01"; volume: "43"; issue: "1"; pages: "132-150"
COMMENT     Sequence Label: pHR-SIN-BX SpyCatcher003-hCD52
FEATURES             Location/Qualifiers
     source          1..9480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     150..168
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(206..228)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     328..347
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     561..580
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        870..888
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     870..887
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             913..1146
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     1240..1256
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1241..1257
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1260..1848
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1366..1377
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1775..1795
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2034..2372)
                     /codon_start=1
                     /product="improved SpyCatcher variant that reacts with 
                     SpyTag003 at a rate approaching the diffusion limit (Keeble
                     et al., 2019)"
                     /label=SpyCatcher003
                     /translation="VTTLSGLSGEQGPSGDMTTEEDSATHIKFSKRDEDGRELAGATME
                     LRDSSGKTISTWISDGHVKDFYLYPGKYTFVETAAPDGYEVATPIEFTVNEDGQVTVDG
                     EATEGDAHT"
     sig_peptide     complement(2376..2435)
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     regulatory      2432..2441
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2461..2868)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    3021..3138
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3423..3467)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3652..3885
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4382..4507
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4554..5187
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5355..5489
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5379..5398)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5433..5452
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5914..5934)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6064..6129
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(7263..7592)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7277..7412
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7577..7597
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(7667..7684)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7838..8426)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7918..7937)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8597..9388,9389..9457))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9220..9239
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9458..9480,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
       61 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
      121 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
      181 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
      241 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
      301 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
      361 attcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
      421 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
      481 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
      541 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
      601 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tctggctagc gatgaccctg
      661 ctgattggtt cgctgaccat ttccgggtgc gggacggcgt taccagaaac tcagaaggtt
      721 cgtccaacca aaccgactct gacggcagtt tacgagagag atgatagggt ctgcttcagt
      781 aagccagatg ctacacaatt aggcttgtac atattgtcgt tagaacgcgg ctacaattaa
      841 tacataacct tatgtatcat acacatacga tttaggtgac actatagaat acacggaatt
      901 aattctagat gctgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
      961 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
     1021 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
     1081 accagagaga cccagtacaa gcaaaaagca gatcttgtct tcgttgggag tgaattagcc
     1141 cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca
     1201 ttggtcttaa aggtaccgag ctcgaattaa ttccctcgat cgaggtcgac ggtatcgatg
     1261 cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa gacgcggaag
     1321 aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga gggccgaagg
     1381 gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga gcagccaagg
     1441 aaaggacgat gatttccccg acaacaccac ggaattgtca gtgcccaaca gccgagcccc
     1501 tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag ggagggggaa
     1561 agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag tgggggttgc
     1621 gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca actcctcata
     1681 aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac gggaagcaat
     1741 agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag caacatagtt
     1801 aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt
     1861 gatatcaagc ttgcatgcct gcaggtcgac tctagagtcg cggccgccta actgaagcag
     1921 aagaggtgga ttatggcatt ggccacgaag aaaaggaaaa tgcctccgct tatgttgctg
     1981 gatgctgagg ggctgctggt ttggctggtg cttccaccag atccgctaga tccggtgtgt
     2041 gcatcgccct ctgttgcctc tccatccacg gtcacctgtc cgtcctcgtt cactgtaaac
     2101 tcgatagggg ttgccacctc atatccgtct ggtgctgctg tctccacaaa ggtgtacttg
     2161 ccgggataca ggtagaaatc cttcacgtgg ccgtcggaga tccatgtgct gatggtcttg
     2221 ccggagctat ccctcagctc catggttgct cctgccagct ctcttccgtc ctcgtccctc
     2281 ttgctgaact tgatgtgtgt ggcagaatcc tcctctgtgg tcatgtcgcc ggatggtccc
     2341 tgctctccag acagtccgga cagtgtggtc acgtcaccag tggaacctgg aacccagagc
     2401 agcagtaccc atagcaggag tgtgtctgtc tccatggtgg cctcgagtac cgggatcccc
     2461 cccgggcgac tcagtctgtc ggaggactgg cgcgccgagt gaggggttgt gagctctttt
     2521 atagagctcg ggaagcagaa gcgcgcgaac agaagcgaga agcaggctga ttggttaatt
     2581 caaataaggc gcagggtcat ttcaggtcct tgggggagcc tggaaacatc tgatgggtct
     2641 taagaaactg ctgagggttg ggccatatct ggggaccatc tgttcttggc ctcgggccgg
     2701 ggccgaaact gcggtgacca tctgttcttg gccccgggcc ggggccgaaa ctgctcaccg
     2761 cagatatcct gtttggccca acgttagcta ttttcatgta cccgcccttg atctgaactt
     2821 ctctattctt ggtttggtat ttttccatgc cttgcaaaat ggcgttactg cagctagctt
     2881 gccaaaccta caggtggggt ctttcattcc cccctttttc tggagactaa ataaaatctt
     2941 ttattttatc ggggctgcag gaattcgata tcaagcttat cgatccaaac tggatctctg
     3001 ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg taattcttta
     3061 gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta ccccccaatc
     3121 cccccttttc ttttaaaatt gtggatgaat actgccattt ggcgataccg tcgagatccg
     3181 ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg
     3241 ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc
     3301 tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct
     3361 cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc
     3421 cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt
     3481 tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac
     3541 tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc
     3601 caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt
     3661 tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag
     3721 actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc
     3781 tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc
     3841 atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt
     3901 ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt
     3961 tttactactt tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca
     4021 ggtctgaaga tcagcggccg gccgcttgct gtgcggtggt cttacttttg ttttgctctt
     4081 cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat
     4141 agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg
     4201 gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa
     4261 ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct
     4321 ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac
     4381 tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac
     4441 tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc
     4501 gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga
     4561 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     4621 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     4681 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
     4741 ggcaaaaagc agctgcttat atgcaggatc tgagggctcg ccactcccca gtcccgccca
     4801 ggccacgcct ccctggaaag tccccagcgg aaagtccctt gtagcaagct cgatatcagc
     4861 agttcttgaa gtactccgga tgcagctctc gggccacgtg atgaaatgct aggcggctgt
     4921 caaacctcca ctctaacact tctctctccg ggtcatccat cccatgcagg ctcacagggt
     4981 gtaacaagct ggtgttctct cctttattgg cctcttctac cttatctggc tcaactggta
     5041 ctagcttgta gcaccatcca aaggtcagtg gatatctgac ccctggccct ggtgtgtagt
     5101 tctgctaatc agggaagtag ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc
     5161 ttctttggga gtgaattagc ccttccaact actaagtttg tagtacatat ttaacaaata
     5221 caatttcttt aaaatgaaaa taattcagag gaatcacagg tttagagtaa atgaaaccac
     5281 aggtaattgg cagtggtaat agggtatggg gtgggaagtt tgggatgatt ttggttagct
     5341 tgagttatcc agttgatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     5401 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     5461 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     5521 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     5581 tggctgatta tgatctctag tcaaggcact atacatcaaa tattccttat taaccccttt
     5641 acaaattaaa aagctaaagg tacacaattt ttgagcatag ttattaatag cagacactct
     5701 atgcctgtgt ggagtaagaa aaaacagtat gttatgatta taactgttat gcctacttat
     5761 aaaggttaca gaatattttt ccataatttt cttgtatagc agtgcagctt tttcctttgt
     5821 ggtgtaaata gcaaagcaag caagagttct attactaaac acagcatgac tcaaaaaact
     5881 tagcaattct gaaggaaagt ccttggggtc ttctaccttt ctcttctttt ttggaggagt
     5941 agaatgttga gagtcagcag tagcctcatc atcactagat ggcatttctt ctgagcaaaa
     6001 caggttttcc tcattaaagg cattccacca ctgctcccat tcatcagttc cataggttgg
     6061 aatctaaaat acacaaacaa ttagaatcag tagtttaaca cattatacac ttaaaaattt
     6121 tatatttacc ttagagcttt aaatctctgt aggtagtttg tccaattatg tcacaccaca
     6181 gaagtaaggt tccttcacaa agatccgggg cccactcata aatccagttg ccgccacggt
     6241 agccaatcac cgtatcgtat aaatcatcgt cggtacgttc ggcatcgctc atcacaatac
     6301 gtgcctggac gtcgaggatt tcgcgtgggt caatgccgcg ccagatccac atcagacggt
     6361 taatcatgcg ataccagtga gggatggttt taccatcaag ggccgactgc acaggcggtt
     6421 gtgcgccgtg attaaagcgg cggactagcg tcgaggtttc aggatgttta aagcggggtt
     6481 tgaacagggt ttcgctcagg tttgcctgtg tcatggatgc agcctccaga atacttactg
     6541 gaaactattg taacccgcct gaagttaaaa agaacaacgc ccggcagtgc caggcgttga
     6601 aaagattagc gaccggagat tggcgggacg aatacgacgc ccatatccca cggctgttca
     6661 atccaggtat cttgcgggat atcaacaaca tagtcatcaa ccagcggacg accagccggt
     6721 tttgcgaaga tggtgacaaa gtgcgctttt ggatacattt cacgaatcgc aaccgcagta
     6781 ccaccggtat ccaccaggtc atcaataacg atgaagcctt cgccatcgcc ttctgcgcgt
     6841 ttcagcactt taagctcgcg ctggttgtcg tgatcgtagc tggaaataca aacggtatcg
     6901 acatgacgaa tacccagttc acgcgccagt aacgcacccg gtacagaccg ccacggctta
     6961 cggcaataat gcctttccat tgttcagaag gcatcagtcg gcttgcgagt ttacgtgcat
     7021 ggatctgcaa catgtcccag gtgacgatgt atttttcgct catgtgaagt gtcccagcct
     7081 gtttatctac ggcttaaaaa gtgttcgagg ggaaaatagg ttgcgcgaga ttatagagat
     7141 ctggcgcact aaaaaccagt atttcacatg agtccgcgtc tttttacgca ctgcctctcc
     7201 ctgacgcggg ataaagtggt attctcaaac atatctcgca agcctgtctt gtgtccaagc
     7261 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     7321 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     7381 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     7441 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     7501 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     7561 cctggggact ttccacaccc taactgacac acattccaca gctgcattaa tgaatcggcc
     7621 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7681 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     8281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8401 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8521 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8581 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8641 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8701 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8761 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8821 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8881 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8941 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9001 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9061 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9121 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9181 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9241 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9301 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9361 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9421 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
//
