LOCUS       sequence-417709-       11635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vinculin tension sensor (VinTS) with vinculin S1033 
            unphosphorylatable point mutation (S1033A), in lentiviral expression
            vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Shoyer TC, Gates EM, Cabe JI, Urs AN, Conway DE, Hoffman BD
  TITLE     Coupling during collective cell migration is controlled by a 
            vinculin mechanochemical switch.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Dec 12;120(50):e2316456120. doi: 
            10.1073/pnas.2316456120. Epub 2023 Dec 6.
  PUBMED    38055737
REFERENCE   2  (bases 1 to 11635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-12-12"; volume: "120(50):e2316456120. 
            doi"; pages: " 10.1073/pnas.2316456120. Epub 2023 Dec 6"
COMMENT     Sequence Label: pRRL-VinculinTS-S1033A
FEATURES             Location/Qualifiers
     source          1..11635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     123..143
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        217..235
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2929..3636
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(3757..3759,3760..3762,3763..4473)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3802..3823)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4063..4082)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4410..4431
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5086..5674
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5139..5159)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5557..5568)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5765..5998
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6070..6191
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6107..6126)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6161..6180
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6231..6366
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6387..6405)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6415..6432)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6415..6431)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6424..6446)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6573..7028
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6660..6679)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6870..6891
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7054..7158
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7159..7227,7228..8019)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7377..7396)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8190..8778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8679..8698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8932..8949
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9066..9087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9102..9107,9108..9125,9126..9132)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9140..9156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9145..9167
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9164..9180
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9164..9180
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9199..9219
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9201..9219
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9247..9473
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9474..9654
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9701..9826
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10319..10552
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10737..10781
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11048..11164
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        11224..11603
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(11604..11635,1..172)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
       61 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      121 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct
      181 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
      241 ccaagctggc tagcgtttaa acttaagctt gccatgcccg tcttccacac gcgcaccatc
      301 gagagcatct tggagcccgt ggctcagcag atctcccacc tggtcatcat gcacgaggag
      361 ggggaggtag acggcaaggc catcccggac ctcaccgccc ccgtgtcggc cgtgcaggcc
      421 gctgtcagca acctggtgcg ggttggaaaa gaaactgtgc agacaacaga agaccagatc
      481 ttgaaaaggg atatgccacc agcattcatc aaagtagaga atgcctgcac caagctcgtt
      541 cgagcagccc agatgctgca agcagatcct tattcagtac cagctcgtga ctacctaatt
      601 gatggatcaa gaggcatcct ttctggaaca tcagacttac ttctgacatt tgatgaagcg
      661 gaggtccgta aaatcatccg tgtctgcaaa ggaatattgg aatatctgac tgtggcagaa
      721 gtagtagaga ctatggagga tttggtgaca tatacaaaga atctagggcc aggaatgaca
      781 aagatggcga aaatgatcga tgagagacaa caggaattaa ctcatcagga acatagggtt
      841 atgctggtga actccatgaa tactgtgaag gagctattgc ctgtacttat ttcagctatg
      901 aagatctttg taaccacaaa aaacactaaa agccagggaa tagaagaggc cttgaaaaat
      961 cgcaatttca cagtagagaa aatgagtgct gagataaatg aaataatccg tgtattacaa
     1021 ctcacttcct gggatgaaga tgcctgggcc agcaaggaca ctgaagccat gaaaagagct
     1081 ttagccctaa tagattcaaa gatgaaccag gcaaaaggct ggctgagaga tccaaacgca
     1141 cctccagggg atgctggtga gcaagcaata aggcagatcc ttgatgaagc tggaaaagca
     1201 ggagaattgt gtgcaggcaa agaacgcagg gagattctgg gaacatgcaa aactttgggc
     1261 caaatgactg atcaacttgc tgatctccga gctagaggac agggtgctac accgatggca
     1321 atgcagaagg cacagcaggt gtcacaaggc ctggatttgc tcactgcaaa agtggagaat
     1381 gcagcccgga aattggaggc catgacaaac tctaagcagg ctattgcaaa gaagattgat
     1441 gctgctcaga actggcttgc ggatcccaac ggcggaagtg aaggagaaga acacattcga
     1501 ggaattatgt ctgaagcaag gaaagttgca gaattgtgtg aggagcctaa agaaagagat
     1561 gatatccttc gctccttggg ggaaatctct gctctgacag ctaagctgtc agatctgcga
     1621 cgacatggga aaggcgactc tcctgaggcc cgtgcattgg ccaagcaaat agctacatca
     1681 cttcagaact tacagtccaa aacaaacaga gctgtagcaa atactaggcc agttaaagct
     1741 gctgtccatt tggagggcaa gattgagcaa gcccagaggt ggatagataa tcctacagtt
     1801 gatgatcggg gagtaggcca ggcagcaatt cggggtttgg ttgcagaagg tcgtcgtcta
     1861 gccaatgtca tgatgggacc ttatcgtcag gacctgcttg ccaaatgtga ccgtgtagac
     1921 cagctggctg ctcagcttgc tgaccttgca gcaagagggg aaggagagtc tcctcaggct
     1981 agggcaattg ctgctcagct tcaggactcc ctgaaggatc tcaaagcacg gatgcaagaa
     2041 gcaatgaccc aggaggtgtc tgatgttttc agtgacacta caacccctat taagttgtta
     2101 gcagtagcag ccactgctcc ttctgatact cccaatagag aagaggtgtt tgaagaaaga
     2161 gcagcaaatt ttgaaaacca tgctgctaga ctgggagcaa cagcagaaaa agcagctgca
     2221 gttggaactg ctaataaaac tactgtggaa ggcattcagg caacagtcaa atctgcaagg
     2281 gagcttacac cacaggtagt atcggctgct cgaatcctcc tgagaaatcc tggaaatcaa
     2341 gctgcttatg agcattttga gacaatgaaa aaccaatgga ttgataatgt agaaaagatg
     2401 acagggctgg tggatgaggc catcgatacc aagtctctgt tggatgcatc agaagaggct
     2461 attaagaagg atcttgataa atgtaaagtt gcaatggcca atatgcaacc tcagatgctg
     2521 gtagctggag ccaccagcat tgctagacga gcgaaccgca tcctgcttgt ggcaaaacgg
     2581 gaggttgaaa attcagaaga ccctaaattc agggaggctg ttaaagcagc ttctgatgag
     2641 ctgagcaaaa ccatatcacc gatggtaatg gatgctaaag ctgtagcagg aaatatctct
     2701 gatcctggtt tgcagaagag tttcttggat tctggataca ggattctggg agctgtggcc
     2761 aaagtcagag aagcctttca gcctcaggag ccagactttc cccctcctcc tcctgaccta
     2821 gagcatctcc atctgactga tgagcttgct cctccaaaac caccacttcc agaaggtgag
     2881 gttcccccac ccagaccacc accccctgaa gaaaaagatg aggtcgagat ggtgagcaag
     2941 ggcgaggaga ccacaatggg cgtaatcaag cccgacatga agatcaagct gaagatggag
     3001 ggcaacgtga atggccacgc cttcgtgatc gagggcgagg gcgagggcaa gccctacgac
     3061 ggcaccaaca ccatcaacct ggaggtgaag gagggagccc ccctgccctt ctcctacgac
     3121 attctgacca ccgcgttcgc ctacggcaac agggccttca ccaagtaccc cgacgacatc
     3181 cccaactact tcaagcagtc cttccccgag ggctactctt gggagcgcac catgaccttc
     3241 gaggacaagg gcatcgtgaa ggtgaagtcc gacatctcca tggaggagga ctccttcatc
     3301 tacgagatac acctcaaggg cgagaacttc ccccccaacg gccccgtgat gcagaagaag
     3361 accaccggct gggacgcctc caccgagagg atgtacgtgc gcgacggcgt gctgaagggc
     3421 gacgtcaagc acaagctgct gctggagggc ggcggccacc accgcgttga cttcaagacc
     3481 atctacaggg ccaagaaggc ggtgaagctg cccgactatc actttgtgga ccaccgcatc
     3541 gagatcctga accacgacaa ggactacaac aaggtgaccg tttacgagag cgccgtggcc
     3601 cgcaactcca ccgacggcat ggacgagctg tacaaggggc caggtggtgc agggccaggt
     3661 ggtgcagggc caggtggtgc agggccaggt ggtgcagggc ccggtggtgc aggtccaggt
     3721 ggtgcaggtc caggtggtgc aggtccaggt ggtgctatgg tgagcaaggg cgaggagctg
     3781 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     3841 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagctgatc
     3901 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gggctacggc
     3961 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     4021 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     4081 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     4141 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     4201 cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa cttcaagatc
     4261 cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca gaacaccccc
     4321 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaaactg
     4381 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     4441 gggatcactc tcggcatgga cgagctgtac aaggcggccg cggagttccc agagcagaaa
     4501 gcaggagaag ctattaatca gcccatgatg atggctgcta ggcagttgca tgacgaggct
     4561 cggaaatggt ctagcaaggg taacgacatc attgctgctg ctaaacgaat ggcgctgctc
     4621 atggctgaga tgtcacgcct ggtgcgagga ggcagcggaa acaagcgtgc cctcatccag
     4681 tgtgcaaaag atattgctaa ggcatcggat gaagtcactc gattggccaa agaggtggcc
     4741 aagcaatgta ctgataaacg cattagaaca aacctcttac aggtctgtga gcgaatccca
     4801 accatcagca cgcagctcaa aattctttcc acagtgaaag ctaccatgct gggcaggact
     4861 aacatcagcg atgaagaatc agaacaggca actgagatgt tggttcataa cgcccagaac
     4921 ctcatgcagg ctgtgaagga aactgtgaga gaagctgaag cagcatccat taagataaga
     4981 acagatgccg gattcactct gcgctgggtc agaaagaccc catggtatca gtaagaattc
     5041 tgcagatatc cagcacagtg gcggccgctc gagtctagag tcgacaatca acctctggat
     5101 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5161 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5221 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5281 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5341 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5401 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5461 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     5521 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5581 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     5641 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattcgag ctcggtacct
     5701 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5761 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     5821 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5881 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5941 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     6001 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     6061 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6121 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6181 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     6241 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6301 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6361 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     6421 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6481 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6541 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     6601 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6661 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6721 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6781 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6841 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6901 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6961 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     7021 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     7081 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     7141 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7201 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7261 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     7321 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     7381 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     7441 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7501 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7561 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7621 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7681 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7741 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     7801 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     7861 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     7921 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     7981 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     8041 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8101 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8161 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8221 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8281 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     8341 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8401 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8461 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8521 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8581 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8641 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8701 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     8761 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     8821 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     8881 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     8941 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     9001 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     9061 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     9121 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     9181 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     9241 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     9301 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     9361 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     9421 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     9481 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     9541 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     9601 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     9661 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     9721 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     9781 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     9841 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     9901 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     9961 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
    10021 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
    10081 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
    10141 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
    10201 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
    10261 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
    10321 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
    10381 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
    10441 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
    10501 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
    10561 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
    10621 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
    10681 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
    10741 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
    10801 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
    10861 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
    10921 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
    10981 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
    11041 ggttaacttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
    11101 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa
    11161 ttttatcgat ggatcccccg ggctcgagtt cgaagatcga tgtacgggcc agatatacgc
    11221 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
    11281 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
    11341 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
    11401 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
    11461 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
    11521 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
    11581 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcg
//
