LOCUS       sequence-417714-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector that endcodes sgRNAs against mouse Kdm6a, Rb1, Trp53, 
            and Rbl2 and CMV Cre recombinase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Duplaquet L, Li Y, Booker MA, Xie Y, Olsen SN, Patel RA, Hong D, 
            Hatton C, Denize T, Walton E, Laimon YN, Li R, Jiang Y, Bronson RT, 
            Southard J, Li S, Signoretti S, Qiu X, Cejas P, Armstrong SA, Long 
            HW, Tolstorukov MY, Haffner MC, Oser MG
  TITLE     KDM6A epigenetically regulates subtype plasticity in small cell lung
            cancer.
  JOURNAL   Nat Cell Biol. 2023 Sep;25(9):1346-1358. doi: 
            10.1038/s41556-023-01210-z. Epub 2023 Aug 17.
  PUBMED    37591951
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-023-01210-z"; journalName: "Nat Cell Biol"; date: 
            "2023-09"; volume: "25"; issue: "9"; pages: "1346-1358"
COMMENT     Sequence Label: 
            pENTR223-CMV-Cre-U6-sgKdm6a#4-U6-sgRb1-U6-sgTrp53-U6-sgRbl2
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..92
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        171..419
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        440..515
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        535..783
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
ORIGIN
        1 tttaccgnnn nttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
       61 attgattact acaactttgt atacaaaagt tgtggttatt ttggatccac cggtatcgat
      121 gaattcaagc ttggtaccgt cgacgatcca gtttggttcg ttaaggtacc gagggcctat
      181 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa
      241 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
      301 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
      361 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
      421 ccttggctcg acaaaagctg ttttagagct agaaatagca agttaaaata aggctagtcc
      481 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgct agctgagggc
      541 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
      601 agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
      661 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
      721 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
      781 accgcaacta
//
