LOCUS       sequence-417726-        9859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NIR light-activated GSDMD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Karasev MM, Verkhusha VV, Shcherbakova DM
  TITLE     Near-Infrared Optogenetic Module for Conditional Protein Splicing.
  JOURNAL   J Mol Biol. 2023 Dec 15;435(24):168360. doi: 
            10.1016/j.jmb.2023.168360. Epub 2023 Nov 8.
  PUBMED    37949312
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2023-12-15"; volume: "435(24):168360. doi"; pages: " 
            10.1016/j.jmb.2023.168360. Epub 2023 Nov 8"
COMMENT     Sequence Label: NIR-AG
FEATURES             Location/Qualifiers
     source          1..9859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(188..207)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    232..279
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      608..1196
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1097..1116
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        1371..1674
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1675..1878
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1829..1849
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4583..4636
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4637..4639,4640..4642,4643..5356)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4682..4703)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4943..4962)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5290..5311
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(5367..5941,5942..5944,5945..5953)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5534..5551)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5761..5780
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6776..6802
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7163..7204
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    7957..8078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7994..8013)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8048..8067
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8085..8540)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8222..8243)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8434..8453
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8567..8671
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8673..9030
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8673..8864
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8696..8716)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8881..9016
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9065..9859
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9065..9859
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9119..9138)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9729..9748
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
       61 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
      121 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
      181 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
      241 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
      301 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
      361 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
      421 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
      481 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
      541 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
      601 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      661 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      721 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
      781 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      841 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      901 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
      961 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1021 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1081 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1141 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1201 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1261 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat tagttattaa
     1321 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     1381 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     1441 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     1501 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     1561 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     1621 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     1681 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     1741 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     1801 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     1861 gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcacca tgtactccgg
     1921 atactgtctg gatctgaaaa cccaggttca gacaccgcag ggtatgaaag aaatttcaaa
     1981 tattcaggtg ggtgatctgg ttctgagcaa tactggttat aatgaagtgc tgaatgtgtt
     2041 tccgaaaagc aaaaaaaaaa gctataaaat caccctggaa gatggcaaag aaatcatttg
     2101 tagcgaagaa cacctgtttc cgacccagac tggtgaaatg aatattagcg gtggtctgaa
     2161 agaaggtatg tgcctgtatg ttaaagaaat gatgctgaaa aaaatcctga aaatcgagga
     2221 actggatgaa cgcggtacca gtgctggtgg gtccgccggc gggagtgctg gcggtagtgc
     2281 cggcggtagt ctcgagggct ccatgagccg cgaccccctg ccattcttcc cccccctgta
     2341 cctgggagga cccgagatta ccaccgaaaa ctgtgaacga gagcctatcc acatccccgg
     2401 aagtattcag cctcatggcg ccctgctgac tgctgatggc cacagtgggg aagtgctgca
     2461 gatgtcactg aacgccgcta ccttcctggg gcaggagcct actgtgctga ggggacagac
     2521 cctggcagca ctgctgcctg aacagtggcc agcactgcag gctgcactgc cacctggctg
     2581 ccccgatgcc ctgcagtaca gggctactct ggactggcct gcagctggac acctgagcct
     2641 gaccgtccat cgcgtggggg aactgctgat cctggagttc gaaccaacag aggcttggga
     2701 tagcactggc ccccacgcac tgcggaacgc tatgtttgca ctggagtccg cccccaatct
     2761 gagagcactg gccgaagtcg ccactcagac cgtgcgggag ctgacagggt tcgatagagt
     2821 catgctgtac aagtttgccc ctgacgctac tggagaagtg atcgctgaag cacggagaga
     2881 ggggctgcac gctttcctgg gacatcgatt tccagcctcc gacattccag cacaggctcg
     2941 ggcactgtat accaggcacc tgctgcgact gacagcagat actcgcgcag cagctgtccc
     3001 actggacccc gtgctgaacc ctcagaccaa tgcaccaaca cctctgggag gagcagtgct
     3061 gagagcaacc tctccaatgc atatgcagta tctgaggaat atgggagtcg gcagctccct
     3121 gtctgtgagt gtggtcgtgg gaggacagct gtggggactg attgcctgcc accatcagac
     3181 accctacgtg ctgccacccg atctgaggac cacactggag tatctggggc gcctgctgtc
     3241 tctgcaggtc caggtgaaag aagcagccga cgtcgctgca ttccgacaga gtctgcgaga
     3301 gcaccatgca cgggtggccc tggccgctgc acactcactg agccctcatg acacactgag
     3361 cgatccagct ctggacctgc tgggactgat gagagcagga ggactgatcc tgagatttga
     3421 aggcaggtgg cagactctgg gagaggtccc tccagcacca gctgtggacg ctctgctggc
     3481 atggctggag acccagccag gagctctggt gcagacagat gcactgggac agctgtggcc
     3541 agcaggagca gacctggctc cttcagcagc tggactgctg gccatttccg tcggagaagg
     3601 atggtctgag tgtctggtgt ggctgcgccc agaactgcga ctggaggtgg cttggggagg
     3661 agcaacccct gatcaggcaa aggacgatct gggaccacgc cacagctttg acacatacct
     3721 ggaggaaaaa cgaggctatg ccgagccctg gcacccaggg gaaattgagg aggcacagga
     3781 tctgagagac acactgacag gggcactggg ggagagactg tccgtgattc gcgatctcaa
     3841 ccgggctctg acccagtcca atgccgagtg gcgtcagtat ggcttcgtca tcagtcacca
     3901 catgcaggaa cccgtcagac tgatctccca gtttgccgag cttctgactc gccaacccag
     3961 agcccaggat ggcagcccgg actctcccca gacagagcgt atcaccggct ttctgctcag
     4021 ggaaacctca cgactccgct ctctgaccca ggacttgcac acgtacactg cgctgctgtc
     4081 agcacctcct ccagttagga ggccaacacc acttgggcga gttgtggacg atgtgctgca
     4141 ggacctcgaa ccacggattg ccgatacagg ggcctccata gaggtagctc ccgaactgcc
     4201 cgtcattgcc gctgatgctg gcctcttgcg cgaccttctg ctgcatctga ttggtaacgc
     4261 cttgaccttc ggaggaccag agcctcggat tgctgtgaga accgaacggc aaggtgccgg
     4321 ctggagcatc gctgtgagcg atcagggtgc ggggatagcg ccggagtatc aggagaggat
     4381 cttcttgctc ttccagagac tcggctctct ggacgaggca ctgggcaatg gacttgggct
     4441 tcccctgtgt cggaagatcg ccgaactgca tggtggcaca ctgactgtgg agagtgcacc
     4501 tggcgaagga agcacgttta ggtgctggct gcctgatgcc ggaccactgc ctggagcagc
     4561 agacgctgaa ttcggtagcg gcgagggcag aggaagtctg ctaacatgcg gtgatgtcga
     4621 ggagaatcct ggcccaatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     4681 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     4741 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     4801 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     4861 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     4921 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     4981 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     5041 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     5101 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     5161 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     5221 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     5281 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     5341 cgagctgtac aagtaacgta cgtatgcccc tctccctccc ccccccctaa cgttactggc
     5401 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     5461 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     5521 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     5581 gttcctctgg cagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     5641 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     5701 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     5761 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     5821 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     5881 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acggtgataa
     5941 tgtggccaca accatggggt cggcctttga gcgggtagtc cggagagtgg tccaggagct
     6001 ggaccatggt ggggagttca tccctgtgac cagcctgcag agctccactg gcttccagcc
     6061 ctactgcctg gtggttagga agccctcaag ctcatggttc tggaaacccc gttataagtg
     6121 tgtcaacctg tctatcaagg acatcctgga gccggatgcc gcggaaccag acgtgcagcg
     6181 tggcaggagc ttccacttct acgatgccat ggatgggcag atacagggca gcgtggagct
     6241 ggcagcccca ggacaggcaa agatcgcagg cggggccgcg gtgtctgaca gctccagcac
     6301 ctcaatgaat gtgtactcgc tgagtgtgga ccctaacacc tggcagactc tgctccatga
     6361 gaggcacctg cggcagccag aacacaaagt cctgcagcag ctgcgcagcc gcggggacaa
     6421 cgtgtacgtg gtgactgagg tgctacagac acagaaggag gtggaagtca cgcgcaccca
     6481 caagcgggag ggctcgggcc ggttttccct gcccggagcc acgtgcttgc agggtgaggg
     6541 ccagggccat ctgagccaga agaagacggt caccatcccc tcaggcagca ccctcgcatt
     6601 ccgggtggcc cagctggtta ttgactctga cttggacgtc cttctcttcc cggataagaa
     6661 gcagaggacc ttccagccac ccgcgacagg ccacaagcgt tccacgagcg aaggcgcctg
     6721 gccacagctg ccctctggcc tctccatgat gaggtgcctc cacaacttcc tgacataccc
     6781 ttacgatgtg cccgattacg ccagcggagg cggatctacc ggtatgaagt ttaacaagga
     6841 gaagtggcag gctattaagg agattgcctt cctgcctaac ctgaaccagc cccagcacct
     6901 ggcttttatt gtgtctctgc tggacgaccc ttctcagtct gctaacctgc tggctgaggc
     6961 taagaagctg aacgacgctc aggctcctaa gggatccgaa ctgattgata ttgaagttag
     7021 cggtaaccac ctgttctatg ccaatgatat tctgacccat aatagctcgt ccactagtgg
     7081 agggtcaggg gctggagggt cgggggcagg cggttcggga gccggaggat ctggaggggg
     7141 tggttcgggt ggtggtggta gcggcaagcc cattcccaat cccttgctgg ggttggactc
     7201 caccggggtc cctgcggagg gggcgttcac tgaagacttc cagggcctac gggcagaggt
     7261 ggagaccatc tccaaggaac tggagctttt ggacagagag ctgtgccagc tgctgctgga
     7321 gggcctggag ggggtgctgc gggaccagct ggccctgcga gccttggagg aggcgctgga
     7381 gcagggccag agccttgggc cggtggagcc cctggacggt ccagcaggtg ctgtcctgga
     7441 gtgcctggtg ttgtcctccg gaatgctggt gccggaactc gctatccctg ttgtctacct
     7501 gctgggggca ctgaccatgc tgagtgaaac gcagcacaag ctgctggcgg aggcgctgga
     7561 gtcgcagacc ctgttggggc cgctcgagct ggtgggcagc ctcttggagc agagtgcccc
     7621 gtggcaggag cgcagcacca tgtccctgcc ccccgggctc ctggggaaca gctggggcga
     7681 aggagcaccg gcctgggtct tgctggacga gtgtggccta gagctggggg aggacactcc
     7741 ccacgtgtgc tgggagccgc aggcccaggg ccgcatgtgt gcactctacg cctccctggc
     7801 actgctatca ggactgagcc aggagcccca ctaaagcggc cgcgactcta gatcataatc
     7861 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     7921 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     7981 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8041 tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa
     8101 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     8161 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     8221 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     8281 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     8341 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     8401 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     8461 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     8521 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8581 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8641 aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat
     8701 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8761 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8821 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     8881 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8941 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     9001 ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt
     9061 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     9121 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     9181 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     9241 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     9301 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     9361 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     9421 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     9481 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     9541 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     9601 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     9661 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     9721 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     9781 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     9841 tcttgacgag ttcttctga
//
