LOCUS       sequence-417730-        8706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of LifeAct-mNeonGreen and Talin head(1-433)-SpyTag003 in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8706)
  AUTHORS   Rahikainen R, Vester SK, Turkki P, Janosko CP, Deiters A, Hytonen 
            VP, Howarth M
  TITLE     Visible Light-Induced Specific Protein Reaction Delineates Early 
            Stages of Cell Adhesion.
  JOURNAL   J Am Chem Soc. 2023 Nov 15;145(45):24459-24465. doi: 
            10.1021/jacs.3c07827. Epub 2023 Oct 31.
  PUBMED    38104267
REFERENCE   2  (bases 1 to 8706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.3c07827"; journalName: "J Am Chem Soc"; date: 
            "2023-11-15- 15"; volume: "145"; issue: "45"; pages: "24459-24465"
COMMENT     Sequence Label: LifeAct-mNeonGreen -IRES- Talin head-SpyTag003
FEATURES             Location/Qualifiers
     source          1..8706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(15..229)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(75..94)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        complement(340..580)
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     complement(390..409)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(560..580)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(588..599)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     enhancer        670..1049
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1050..1253
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1204..1224
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1298..1317
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1298..1316
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1352..1361
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1358..1408
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     misc_feature    join(2158..2731,2732..2734,2735..2743)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2325..2342)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2552..2571
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4052..4099
                     /codon_start=1
                     /product="improved SpyTag variant that reacts with 
                     SpyCatcher003 at a rate approaching the diffusion limit 
                     (Keeble et al., 2019)"
                     /label=SpyTag003
                     /translation="RGVPHIVMVDAYKRYK"
     promoter        complement(4169..4187)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4170..4187)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4207..4224)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4213..4437
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4483..4911
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4570..4589)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4780..4801
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4920..4940)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4925..5254
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5105..5240
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5167..5186
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5321..6115
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5375..5394)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5985..6004
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6289..6410
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6326..6345)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6380..6399
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6459..6475)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6459..6475)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6472..6494)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6483..6499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6507..6513,6514..6531,6532..6537))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6552..6573
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6690..6707)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6861..7449)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6941..6960)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7620..8411,8412..8480))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8243..8262
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8481..8585)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aggttcccag agctgtggtc tcatacagaa cttataagat tcccaaatcc aaagacattt
       61 cacgtttatg gtgatttccc agaacacata gcgacatgca aatattgcag ggcgccactc
      121 ccctgtccct cacagccatc ttcctgccag ggcgcacgcg cgctgggtgt tcccgcctag
      181 tgacactggg cccgcgattc cttggagcgg gttgatgacg tcagcgttcg aattgactag
      241 caaaaaccgc acttgtcaga tccaaaaaac ggggagtacg ggagtttcac ccgcctgcac
      301 gaatttagag tccgtgtgat ctattcgatc aacccccccg tcctttccac aagatatata
      361 aagccaagaa atcgaaatac tttcaagtta cggtaagcat atgatagtcc attttaaaac
      421 ataattttaa aactgcaaac tacccaagaa attattactt tctacgtcac gtattttgta
      481 ctaatatctt tgtgtttaca gtcaaattaa ttctaattat ctctctaaca gccttgtatc
      541 gtatatgcaa atatgaagga atcatgggaa ataggccctc ttcctgcccg accttcaatt
      601 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
      661 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      721 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      781 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      841 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      901 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      961 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1021 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     1081 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     1141 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     1201 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
     1261 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
     1321 cccaagcttg gtaccgagct cggatcccgc tgccaccatg ggcgtggccg acctgatcaa
     1381 gaagttcgag agcatcagca aggaggaggg ggatccaccg gtcgcaacta tggtgtccaa
     1441 gggcgaagag gacaacatgg ccagcctgcc tgctacacac gagctgcaca tcttcggcag
     1501 catcaacggc gtggacttcg acatggtcgg acaaggcaca ggcaacccca acgacggata
     1561 cgaggaactg aacctgaagt ccacaaaggg cgacctgcag ttcagcccct ggatcctggt
     1621 gcctcacatc ggctacggct tccaccagta cctgccttat cctgacggca tgagcccttt
     1681 ccaggccgct atggtggatg gctctggata ccaggtgcac agaaccatgc agttcgagga
     1741 cggcgccagc ctgaccgtga actacagata cacatacgag ggcagccaca tcaaaggcga
     1801 ggcccaagtg aaaggcaccg gctttccagc tgatggcccc gtgatgacca actctctgac
     1861 agccgccgac tggtgcagaa gcaagaaaac ataccctaac gacaagacca tcatcagcac
     1921 cttcaagtgg tcctacacca ccggcaacgg caagagatac agaagcaccg ccagaaccac
     1981 ctacaccttc gccaagccta tggccgccaa ctacctgaag aaccagccta tgtacgtgtt
     2041 ccgcaagacc gagctgaagc actccaagac agaactgaac ttcaaagagt ggcagaaagc
     2101 cttcaccgac gtgatgggca tggacgagct gtacaagtaa taatgacccg ggatccgccc
     2161 ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     2221 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     2281 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     2341 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     2401 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     2461 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     2521 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     2581 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     2641 atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac
     2701 gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggttg cgctttcgct
     2761 gaagattagc attgggaatg tggtgaagac gatgcaattt gagccatcta ccatggtgta
     2821 cgacgcctgc cgcatgattc gtgagcggat cccagaggcc ctggctggcc ctcccaacga
     2881 ctttgggctc tttctgtcag atgatgaccc caaaaaaggc atctggctag aggctgggaa
     2941 agctttggac tactacatgc tccgaaatgg ggacaccatg gagtacagaa aaaagcagag
     3001 acccctgaag atccggatgt tagacggaac ggtgaagact atcatggtgg atgactccaa
     3061 gaccgtcaca gacatgctca tgaccatttg tgcccgaatt ggtatcacca accatgatga
     3121 gtattcactg gttcgagagc tgatggaaga aaagaaagat gaggggacag ggaccctgag
     3181 aaaagacaag accctactgc gagatgaaaa gaagatggag aagctaaagc agaagttgca
     3241 cacagacgat gagttgaact ggctggacca tgggcggaca ctgagggaac agggagtgga
     3301 agagcatgag acgttgctgc tgcggagaaa gttcttctac tcagaccaga atgtggattc
     3361 ccgagaccct gtacagctga accttctcta tgtgcaggca cgagatgaca tcctgaatgg
     3421 ctcccatcct gtctcctttg acaaggcctg tgaatttgca ggcttccagt gccagatcca
     3481 gtttggacct cacaatgaac agaagcacaa ggctggcttc cttgacctga aggacttcct
     3541 gcccaaggag tatgtgaagc agaagggaga gcgtaagatc tttcaggcac acaagaattg
     3601 tgggcagatg agtgaaattg aggccaaggt acgctacgtg aagctggccc gttccctcaa
     3661 gacttacggt gtctccttct tcctagtcaa ggaaaagatg aaggggaaga ataaactggt
     3721 gcccaggctg ttgggcatca ctaaggagtg tgtgatgcgt gtggatgaga agaccaagga
     3781 ggtgattcag gaatggagcc tcaccaacat caaacgctgg gctgcctctc ccaagagctt
     3841 tactctggac tttggagact atcaggatgg ctactactca gtacagacga cagaaggcga
     3901 gcagatcgca cagctcattg ctggctacat agatatcatc cttaagaaga aaaaaagcaa
     3961 ggaccatttt gggctggagg gagatgaaga gtctactatg ttggaggact cagtgtcacc
     4021 caaaaagtca acagtccttc agggaagcgg ccgtggcgtg cctcatatcg tgatggtgga
     4081 cgcctacaag cgttacaagt aatgataaga attctgcaga tatccatcac actggcggcc
     4141 gctcgagcat gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct
     4201 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     4261 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     4321 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     4381 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     4441 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     4501 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     4561 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4621 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4681 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4741 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4801 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     4861 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     4921 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4981 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     5041 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     5101 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5161 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     5221 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     5281 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     5341 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     5401 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     5461 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     5521 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     5581 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     5641 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     5701 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     5761 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     5821 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     5881 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     5941 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     6001 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     6061 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     6121 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     6181 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     6241 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     6301 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6361 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     6421 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6481 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6541 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6601 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6661 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     6721 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     6781 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     6841 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6901 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6961 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7021 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7081 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7141 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7201 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7261 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7321 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7381 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7441 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7501 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7561 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7621 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7681 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     7741 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     7801 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     7861 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     7921 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7981 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8041 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8101 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8161 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8221 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8281 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8341 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8401 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8461 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8521 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8581 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctagatcca
     8641 aaaaacggaa accccgggaa tctaacccgg ctgaacggat ttagagtccg ttcgatctac
     8701 atgatc
//
