LOCUS       sequence-417732-       10223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian overexpression vector for polycistronic co-expression of 
            C-terminally Strep and His tagged human Prom1s1 (WT) and 
            C-terminally Myc and Flag tagged human Pchd21 (WT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10223)
  AUTHORS   Bell TA, Luce BE, Hakim P, Ananda VY, Dardari H, Nguyen TH, 
            Monshizadeh A, Chao LH
  TITLE     Prominin 1 and Tweety Homology 1 both induce extracellular vesicle 
            formation.
  JOURNAL   Elife. 2024 Aug 13;13:e100061. doi: 10.7554/eLife.100061.
  PUBMED    39168849
REFERENCE   2  (bases 1 to 10223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-08-13"; volume: "13:e100061. doi"; pages: " 
            10.7554/eLife.100061"
COMMENT     Sequence Label: pCS2-Prom1-TEVp-TwinStrep-His / IRES2 /
            Pcdh21...
FEATURES             Location/Qualifiers
     source          1..10223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(80..100)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(80..98)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(119..135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(119..135)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(132..154)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    143..159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(167..173,174..191,192..197))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    212..233
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(350..367)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(521..1109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(601..620)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1280..2071,2072..2140))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1903..1922
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2141..2245)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2271..2726)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2408..2429)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2620..2639
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2797..3781
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        3816..3834
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3816..3833
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             6447..6467
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(6480..6485,6486..6509,6510..6545,6546..6569)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             6585..6614
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     misc_feature    join(6695..7269,7270..7272,7273..7281)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6862..6879)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7089..7108
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9874..9894
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             9913..9942
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9958..10023
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    10056..10190
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10080..10099)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10134..10153
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gttcaggggg aggtgtggga ggttttttaa ttcgcggccg cggcgccaat gcattgggcc
       61 cggtacccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
      121 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
      181 ggagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
      241 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
      301 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
      361 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
      421 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
      481 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      541 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      601 aaagatacca ggcgtttccc actggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      661 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      721 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      781 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      841 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      901 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      961 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1021 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1081 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1141 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     1201 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     1261 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     1321 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1381 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1441 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1501 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1561 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     1621 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1681 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1741 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1801 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1861 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     1921 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1981 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     2041 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     2101 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     2161 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     2221 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     2281 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     2341 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     2401 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     2461 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     2521 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     2581 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     2641 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     2701 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     2761 agggcgatcg gtgcgggcct cttcgctatt acgccagtcg accgccaatt caatatggcg
     2821 tatatggact catgccaatt caatatggtg gatctggacc tgtgccaatt caatatggcg
     2881 tatatggact cgtgccaatt caatatggtg gatctggacc ccagccaatt caatatggcg
     2941 gacttggcac catgccaatt caatatggcg gacctggcac tgtgccaact ggggaggggt
     3001 ctacttggca cggtgccaag tttgaggagg ggtcttggcc ctgtgccaag tccgccatat
     3061 tgaattggca tggtgccaat aatggcggcc atattggcta tatgccagga tcaatatata
     3121 ggcaatatcc aatatggccc tatgccaata tggctattgg ccaggttcaa tactatgtat
     3181 tggccctatg ccatatagta ttccatatat gggttttcct attgacgtag atagcccctc
     3241 ccaatgggcg gtcccatata ccatatatgg ggcttcctaa taccgcccat agccactccc
     3301 ccattgacgt caatggtctc tatatatggt ctttcctatt gacgtcatat gggcggtcct
     3361 attgacgtat atggcgcctc ccccattgac gtcaattacg gtaaatggcc cgcctggctc
     3421 aatgcccatt gacgtcaata ggaccaccca ccattgacgt caatgggatg gctcattgcc
     3481 cattcatatc cgttctcacg ccccctattg acgtcaatga cggtaaatgg cccacttggc
     3541 agtacatcaa tatctattaa tagtaacttg gcaagtacat tactattgga agtacgccag
     3601 ggtacattgg cagtactccc attgacgtca atggcggtaa atggcccgcg atggctgcca
     3661 agtacatccc cattgacgtc aatggggagg ggcaatgacg caaatgggcg ttccattgac
     3721 gtaaatgggc ggtaggcgtg cctaatggga ggtctatata agcaatgctc gtttagggaa
     3781 ccgccattct gcctggggac gtcggagcaa gcttgattta ggtgacacta tagaatacaa
     3841 gctacttgtt ctttttgcag gatcccatcg atatggccct cgtactcggc tccctgttgc
     3901 tgctggggct gtgcgggaac tccttttcag gagggcagcc ttcatccaca gatgctccta
     3961 aggcttggaa ttatgaattg cctgcaacaa attatgagac ccaagactcc cataaagctg
     4021 gacccattgg cattctcttt gaactagtgc atatctttct ctatgtggta cagccgcgtg
     4081 atttcccaga agatactttg agaaaattct tacagaaggc atatgaatcc aaaattgatt
     4141 atgacaagat tgtctactat gaagcaggga ttattctatg ctgtgtcctg gggctgctgt
     4201 ttattattct gatgcctctg gtggggtatt tcttttgtat gtgtcgttgc tgtaacaaat
     4261 gtggtggaga aatgcaccag cgacagaagg aaaatgggcc cttcctgagg aaatgctttg
     4321 caatctccct gttggtgatt tgtataataa taagcattgg catcttctat ggttttgtgg
     4381 caaatcacca ggtaagaacc cggatcaaaa ggagtcggaa actggcagat agcaatttca
     4441 aggacttgcg aactctcttg aatgaaactc cagagcaaat caaatatata ttggcccagt
     4501 acaacactac caaggacaag gcgttcacag atctgaacag tatcaattca gtgctaggag
     4561 gcggaattct tgaccgactg agacccaaca tcatccctgt tcttgatgag attaagtcca
     4621 tggcaacagc gatcaaggag accaaagagg cgttggagaa catgaacagc accttgaaga
     4681 gcttgcacca acaaagtaca cagcttagca gcagtctgac cagcgtgaaa actagcctgc
     4741 ggtcatctct caatgaccct ctgtgcttgg tgcatccatc aagtgaaacc tgcaacagca
     4801 tcagattgtc tctaagccag ctgaatagca accctgaact gaggcagctt ccacccgtgg
     4861 atgcagaact tgacaacgtt aataacgttc ttaggacaga tttggatggc ctggtccaac
     4921 agggctatca atcccttaat gatatacctg acagagtaca acgccaaacc acgactgtcg
     4981 tagcaggtat caaaagggtc ttgaattcca ttggttcaga tatcgacaat gtaactcagc
     5041 gtcttcctat tcaggatata ctctcagcat tctctgttta tgttaataac actgaaagtt
     5101 acatccacag aaatttacct acattggaag agtatgattc atactggtgg ctgggtggcc
     5161 tggtcatctg ctctctgctg accctcatcg tgatttttta ctacctgggc ttactgtgtg
     5221 gcgtgtgcgg ctatgacagg catgccaccc cgaccacccg aggctgtgtc tccaacaccg
     5281 gaggcgtctt cctcatggtt ggagttggat taagtttcct cttttgctgg atattgatga
     5341 tcattgtggt tcttaccttt gtctttggtg caaatgtgga aaaactgatc tgtgaacctt
     5401 acacgagcaa ggaattattc cgggttttgg atacacccta cttactaaat gaagactggg
     5461 aatactatct ctctgggaag ctatttaata aatcaaaaat gaagctcact tttgaacaag
     5521 tttacagtga ctgcaaaaaa aatagaggca cttacggcac tcttcacctg cagaacagct
     5581 tcaatatcag tgaacatctc aacattaatg agcatactgg aagcataagc agtgaattgg
     5641 aaagtctgaa ggtaaatctt aatatctttc tgttgggtgc agcaggaaga aaaaaccttc
     5701 aggattttgc tgcttgtgga atagacagaa tgaattatga cagctacttg gctcagactg
     5761 gtaaatcccc cgcaggagtg aatcttttat catttgcata tgatctagaa gcaaaagcaa
     5821 acagtttgcc cccaggaaat ttgaggaact ccctgaaaag agatgcacaa actattaaaa
     5881 caattcacca gcaacgagtc cttcctatag aacaatcact gagcactcta taccaaagcg
     5941 tcaagatact tcaacgcaca gggaatggat tgttggagag agtaactagg attctagctt
     6001 ctctggattt tgctcagaac ttcatcacaa acaatacttc ctctgttatt attgaggaaa
     6061 ctaagaagta tgggagaaca ataataggat attttgaaca ttatctgcag tggatcgagt
     6121 tctctatcag tgagaaagtg gcatcgtgca aacctgtggc caccgctcta gatactgctg
     6181 ttgatgtctt tctgtgtagc tacattatcg accccttgaa tttgttttgg tttggcatag
     6241 gaaaagctac tgtattttta cttccggctc taatttttgc ggtaaaactg gctaagtact
     6301 atcgtcgaat ggattcggag gacgtgtacg atgatgttga aactataccc atgaaaaata
     6361 tggaaaatgg taataatggt tatcataaag atcatgtata tggtattcac aatcctgtta
     6421 tgacaagccc atcacaacat ctcgaggaga atttgtactt tcaaggcggt ggaggaggga
     6481 gtgcctggtc tcatccacag tttgaaaagg gaggaggatc cggaggtggt agtggcggat
     6541 ctgcatggtc ccaccctcag tttgagaagg gcggcggagg ttctcatcac caccaccatc
     6601 atcatcacca ccactaagct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
     6661 ctgcagtcga cggtaccgcg ggcccgggat tccgcccctc tccctccccc ccccctaacg
     6721 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     6781 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     6841 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     6901 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     6961 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     7021 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     7081 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     7141 cccattgtat gggatctgat ctggggcctc ggtacacatg ctttacatgt gtttagtcga
     7201 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     7261 gatgataata tggccacaac catgcggcgg tgcaggtggg cagcattagc cctgggtctt
     7321 ctacgattgt gtttggccca agctaatttt gctccgcact ttttcgataa cggggttggg
     7381 agtacaaatg gtaatatggc cctgttcagc ttacctgagg atacgccggt tggttctcac
     7441 gtttatacgt taaacggtac tgatcctgag ggtgatccaa tatcctatca tatttcgttt
     7501 gatccgtcca cgcggtcagt cttttcggtc gaccccacat ttggtaacat cactctcgtt
     7561 gaggaactag accgggagcg tgaggacgag atcgaagcga taatatccat ctctgatgga
     7621 ttaaatttgg tagcagaaaa ggtggttatt ctggtaaccg acgctaatga cgaggctcct
     7681 agattcatac aagagccgta cgttgctctc gtaccagaag acataccagc gggatcaatt
     7741 atcttcaaag tccatgctgt cgaccgagat actggatcgg gtgggtctgt cacctatttc
     7801 ctacagaatc tccatagtcc ttttgcggta gatcgtcata gtggcgtcct cagactccaa
     7861 gcgggagcta ccttggacta cgaaaggtcc cgcacccact atataaccgt tgtcgcaaaa
     7921 gacgggggtg ggaggctgca cggcgcagac gtggtatttt cagccacaac cacggtcact
     7981 gtgaacgtgg aggacgtaca ggatatggcc ccggtatttg tgggcacacc gtattacggg
     8041 tatgtttatg aagacacctt accaggttcc gaagtactca aggtggttgc tatggatgga
     8101 gatagaggca aaccgaatcg cattctttac tcactcgtga atggcaacga cggggcattc
     8161 gagattaacg agacgtcggg ggcgatatcg atcacccaaa gtccagcaca gcttcaaaga
     8221 gaagtctacg aattacatgt tcaagtaact gagatgagcc ctgcgggctc gcctgctgcg
     8281 caagctaccg tcccggtgac aattagaatc gtggacctga acaaccatcc cccgacgttt
     8341 tacggagaaa gtggaccaca gaatagattt gaattgagta tgaatgaaca ccccccgcaa
     8401 ggagagattc tgcgaggcct caaaattacg gtaaatgatt cagaccaggg agccaatgcg
     8461 aagtttaatc ttcaacttgt cgggccgcga ggtatttttc gtgtagtacc acagactgtg
     8521 ctgaacgagg cccaggtcac cattatagtc gaaaatagcg cggccatcga tttcgaaaaa
     8581 tcaaaggtgc tcacattcaa gttattagcg gtagaagtaa atacgccgga aaaattctct
     8641 tcgacggctg acgtcgtgat tcaacttttg gacacaaacg ataacgtacc taaatttgac
     8701 tcgctgtatt acgtggcgag gatacctgaa aacgctccag gcggatcttc agtggtagcc
     8761 gttacagcgg ttgacccaga cacgggaccc tggggggagg tgaagtacag cacctatggc
     8821 acgggcgcgg atctcttctt gattcacccg agtaccggct taatctatac gcaaccttgg
     8881 gcttctctag acgccgaagc cactgcacga tacaacttct acgtaaaagc ggaagacatg
     8941 gaagggaagt actcagtggc tgaggtgttc attaccctac tcgatgttaa tgaccaccct
     9001 ccccagttcg gaaagagcgt gcaaaaaaaa acaatggttc taggaactcc tgttaagata
     9061 gaagcaattg atgaagatgc tgaagaaccc aacaatctag ttgattactc gatcacgcac
     9121 gcggagccag caaatgtatt tgatatcaac agtcacacag gggaaatatg gctgaaaaat
     9181 agtataaggt ccctcgatgc actacataat ataacacccg gacgtgactg tctatggtcc
     9241 cttgaggtcc aggccaaaga tcgcggctct ccatccttct ctacaactgc actgctgaag
     9301 atagacatca ctgatgcaga gactctttcg cgctcaccta tggctgcctt tttaatacag
     9361 actaaggata accccatgaa agcggtcggc gttttggccg gtactatggc aaccgttgtg
     9421 gcgatcacag ttttgatcag tacagccact ttctggcgga acaagaaaag caacaaggta
     9481 ttgccgatgc ggcgtgtctt acgcaagagg ccgagtcctg caccccgaac tattcgtatc
     9541 gagtggctca agtcaaaatc tacgaaggca gccacaaagt tcatgttaaa ggagaagccc
     9601 ccaaacgaaa attgcaacaa caactctcct gagtcatccc tactaccaag agctccagcc
     9661 ttgcccccgc ccccctcggt tgcacccagc accggcgctg cacagtggac tgtacctacg
     9721 gtcagcggaa gtctaacgcc gcagcctacc cagcctcccc ctaagcctaa gacgatgggg
     9781 tctccagttc aaagcacact gatctctgag ctaaaacaaa aattcgagaa aaagtctgtg
     9841 cacaataagg cctattttgg tggtggtgga agtgaaaatc tttattttca aggtggaggg
     9901 gggggctccg cagagcagaa gcttataagc gaggaggacc ttggcggcgg tgggtccgac
     9961 tacaaagacc atgatggcga ttataaagat catgacattg attataaaga cgatgatgac
    10021 aaataatcta gaactatagt gagtcgtatt acgtagatcc agacatgata agatacattg
    10081 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
    10141 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
    10201 attgcattca ttttatgttt cag
//
