LOCUS       sequence-417737-        8346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mTurquoise2-inserted PcdhgB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Kanadome T, Hoshino N, Nagai T, Matsuda T, Yagi T
  TITLE     Development of FRET-based indicators for visualizing homophilic 
            trans interaction of a clustered protocadherin.
  JOURNAL   Sci Rep. 2021 Nov 15;11(1):22237. doi: 10.1038/s41598-021-01481-2.
  PUBMED    34782670
REFERENCE   2  (bases 1 to 8346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-11-15"; volume: "11(1):22237. doi"; pages: " 
            10.1038/s41598-021-01481-2"
COMMENT     Sequence Label: pCX:gamma-B2-EC1-G4S-mTQ2-Delta-C6
FEATURES             Location/Qualifiers
     source          1..8346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(1934..1936,1937..2449)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(1976..1997)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2237..2256)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2584..2605
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5368..5387)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5433..5488
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5487..5506)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5849..5865)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5849..5865)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5862..5884)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5873..5889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5897..5903,5904..5921,5922..5927))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5942..5963
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6022..6217
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6068..6203
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6223..6357
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6260..6279)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6314..6333
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6425..6442)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6596..7184)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6676..6695)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7355..8146,8147..8215))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7978..7997
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8216..8320)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggsgsgs gccagggggg gggggggggg gsgagggggg
      541 gggsggggsg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg rgggctcggg ggrggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgccaccat
     1741 gaggaggggc tctgggagcg gtccggcctg gtggctgcag gtactgtcgc ctttcctgtt
     1801 gcttttgttc cacggagctc tcgcggatca gatccgctac tccattcccg aggagctggc
     1861 caagaactcg atagtaggaa acctcgcaag ggatctgggg ctcagtgtcc aggctagcgg
     1921 tggcggtggt tccgtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1981 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     2041 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     2101 ctggcccacc ctcgtgacca ccctgagctg gggcgtgcag tgcttcgccc gctaccccga
     2161 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     2221 taccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     2281 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     2341 cctggggcac aagctggagt acaactactt cagcgacaac gtctatatca ccgccgacaa
     2401 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt
     2461 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     2521 cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga
     2581 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggcg ctagcaatct
     2641 gcccacccgg aagctaaggg tcagcgcaga gaaggattat tttactgtaa atccagagag
     2701 tggggactta cttgtgggtg acagaataga cagagagcag gtttgcggga agcagcctca
     2761 gtgtgttttg gagttcgata ttgtcgctga gaagccctta aatattttcc acgtagctgt
     2821 aattcttcag gatgtgaacg acaacgctcc tctattcaaa cagagtgagg ttgatttaaa
     2881 cattggagag tccaccagac caggtaaagc atttccattg gatccagccc tcgatttaga
     2941 tgctggttct aattctctgc aaaaatacta tctcactgac aatgaatact ttgaagtcac
     3001 ggagaaagag accccagatg gacgtaaata tcctgagttg attctaaaac gttttctgga
     3061 cagagaagag cataattttc accaactggt cctcacggct gtggatgggg gagatcctcc
     3121 ccgaagtggc accacccaga tccgaatcca agtcacagac atcaatgata acccgccaat
     3181 gttcagccag gatgtgttca gtgtcaccct tcgggaggat gtaccaccag gcttctccgt
     3241 gcttcaggtg acagccaccg accaggacga gggtgtcaat gcagaaatca cctacgcttt
     3301 tcacaatgtg gatgaacaag tggaacggat ttttaactta gacaagagaa caggagaaat
     3361 cacgacaaag gataatttgg atttcgagac agctaagagt tacaccctga atgtagaagc
     3421 aaaagatccc ggagacctag catcccattg tagtatccaa gtgaaaattc tggatgagaa
     3481 tgattgtgta ccggaagtaa ttgtgacgtc agtatttacc cccctaccag aagattctcc
     3541 actaggaaca gtcatcgcct tgataaaaac cagagacaga gactctggag aaaatggaga
     3601 cgtctactgc catgtgttgg gaaatgaagg gtttgtattg aaatcttctt cgaagaatta
     3661 ttacaaacta gtgacagaca gaactctaga ccgggaggcg ataccggaat acaacgtcac
     3721 cattgtagct gccgataggg gtaagccccc gctctcctct aacgtaatca tcactctcca
     3781 tatctcagat gtcaacgaca atgcaccggt ttttcaccag gcctcctact tggtccatgt
     3841 ggctgagaac aacccacctg gaacctccat tgctcaagtc agtgcttctg acccggattt
     3901 gggatccaat ggcctcattt cctactccat catagccagc gacctagagc caagggcact
     3961 atcgtccttc gtgtccgtga accaggacag tggagtggtg ttcgcgcagc gcgccttcga
     4021 ccacgagcag ctgcgctcct tccagctgac actacaggcg cgggaccacg gatcgcccac
     4081 actcagtgcc aatgtgagca tgcgtgtgct ggtgggcgac cgcaacgaca acgcgccacg
     4141 cgtgctgtat cccacacttg agcccgacgg ctctgcgctc ttcgacatgg tgccgcgcgc
     4201 tgccgagccc ggatacctgg tcaccaaggt ggtggctgtg gatgcagact ccggacacaa
     4261 tgcctggcta tcttaccacg tgctgcaggc cagcgatccc gggctcttca gcctgggact
     4321 gcgcacaggc gaggtgcgca cagcgcgtgc cctgggagac agggactcag cgcggcagcg
     4381 ccttctggtc gctgtgcgtg atggtggaca gccaccgctc tccgctacag ccacgctgca
     4441 cctcatcttc gctgacagcc tgcaggaggt cctgccagac ctcagcgatg accctctacc
     4501 atctgaccct cagagtgagc ttcagtttta cctggtggtg gccttggccc tgatctcagt
     4561 actcttcctc ctgtcggtga ttctggctat tgccctgcgt ctgagacact cctccagatc
     4621 agctgcctgg ggctgctttc aacctggcct cagctctaac cttgcgactg gagttctccc
     4681 caattacaat gaggggactt taccttattc ctacaatgtg tgtattgcct cacagtcagc
     4741 taagacagag tttaattttc tcaatgtgac tccggaagtt gctcctcagg acctcctctg
     4801 cggcgacgac tcttgggtac caggtactct tggagacact gacgttccgt ttgtctcgga
     4861 ttctatctca aagcaagccc cgcccaacac tgactggcgt ttctctcaag cccagagacc
     4921 cggcacgagc ggatcccaaa atggtgatga aactggcacc tggcccaaca accagtttga
     4981 tacagagatg ctgcaagcca tgatcttggc ctctgccagc gaagctgctg atgggagctc
     5041 taccctggga gggggcgctg gcaccatggg tctgagcgct cgatacggac cccagtttac
     5101 cctgcagcac gtgcctgact accgccagaa cgtgtacatc cctggcagca atgccacgct
     5161 gaccaatgcc gctggcaaac gagatggcaa ggctccagca ggtggtaatg gcaacaagaa
     5221 gaaatcgggc aagaaagaga agaagggcgt tgccatgcca ggtgccgaag atgatgtggt
     5281 gtgagaattc actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa
     5341 tgccctggct cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat
     5401 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     5461 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     5521 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     5581 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     5641 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     5701 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     5761 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     5821 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5881 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     5941 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6001 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6061 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6121 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6181 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct
     6241 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6301 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc
     6361 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     6421 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6481 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6541 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6601 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6661 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6721 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6781 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6841 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6901 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6961 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7021 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7081 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7141 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7201 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7261 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7321 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7381 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7441 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7501 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7561 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7621 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7681 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7741 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7801 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7861 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7921 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7981 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8041 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8101 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8161 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8221 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8281 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8341 cacctg
//
