LOCUS       sequence-417739-        8322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mVenus-inserted PcdhgB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8322)
  AUTHORS   Kanadome T, Hoshino N, Nagai T, Matsuda T, Yagi T
  TITLE     Development of FRET-based indicators for visualizing homophilic 
            trans interaction of a clustered protocadherin.
  JOURNAL   Sci Rep. 2021 Nov 15;11(1):22237. doi: 10.1038/s41598-021-01481-2.
  PUBMED    34782670
REFERENCE   2  (bases 1 to 8322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-11-15"; volume: "11(1):22237. doi"; pages: " 
            10.1038/s41598-021-01481-2"
COMMENT     Sequence Label: pCX:gamma-B2-EC5-Venus-Delta-N3C9-P3
FEATURES             Location/Qualifiers
     source          1..8322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3281..3302)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3542..3561)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3889..3910
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5344..5363)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5409..5464
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5463..5482)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5825..5841)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5825..5841)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5838..5860)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5849..5865
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5873..5879,5880..5897,5898..5903))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5918..5939
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5998..6193
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6044..6179
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6199..6333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6236..6255)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6290..6309
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6401..6418)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6572..7160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6652..6671)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7331..8122,8123..8191))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7954..7973
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8192..8296)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggsgsgc gccaggsggg gggggggggg gggagggggg
      541 gggsggggsg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcck cgggccgggg rgggctcggg ggrggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgccaccat
     1741 gaggaggggc tctgggagcg gtccggcctg gtggctgcag gtactgtcgc ctttcctgtt
     1801 gcttttgttc cacggagctc tcgcggatca gatccgctac tccattcccg aggagctggc
     1861 caagaactcg atagtaggaa acctcgcaag ggatctgggg ctcagtgtcc agaatctgcc
     1921 cacccggaag ctaagggtca gcgcagagaa ggattatttt actgtaaatc cagagagtgg
     1981 ggacttactt gtgggtgaca gaatagacag agagcaggtt tgcgggaagc agcctcagtg
     2041 tgttttggag ttcgatattg tcgctgagaa gcccttaaat attttccacg tagctgtaat
     2101 tcttcaggat gtgaacgaca acgctcctct attcaaacag agtgaggttg atttaaacat
     2161 tggagagtcc accagaccag gtaaagcatt tccattggat ccagccctcg atttagatgc
     2221 tggttctaat tctctgcaaa aatactatct cactgacaat gaatactttg aagtcacgga
     2281 gaaagagacc ccagatggac gtaaatatcc tgagttgatt ctaaaacgtt ttctggacag
     2341 agaagagcat aattttcacc aactggtcct cacggctgtg gatgggggag atcctccccg
     2401 aagtggcacc acccagatcc gaatccaagt cacagacatc aatgataacc cgccaatgtt
     2461 cagccaggat gtgttcagtg tcacccttcg ggaggatgta ccaccaggct tctccgtgct
     2521 tcaggtgaca gccaccgacc aggacgaggg tgtcaatgca gaaatcacct acgcttttca
     2581 caatgtggat gaacaagtgg aacggatttt taacttagac aagagaacag gagaaatcac
     2641 gacaaaggat aatttggatt tcgagacagc taagagttac accctgaatg tagaagcaaa
     2701 agatcccgga gacctagcat cccattgtag tatccaagtg aaaattctgg atgagaatga
     2761 ttgtgtaccg gaagtaattg tgacgtcagt atttaccccc ctaccagaag attctccact
     2821 aggaacagtc atcgccttga taaaaaccag agacagagac tctggagaaa atggagacgt
     2881 ctactgccat gtgttgggaa atgaagggtt tgtattgaaa tcttcttcga agaattatta
     2941 caaactagtg acagacagaa ctctagaccg ggaggcgata ccggaataca acgtcaccat
     3001 tgtagctgcc gataggggta agcccccgct ctcctctaac gtaatcatca ctctccatat
     3061 ctcagatgtc aacgacaatg caccggtttt tcaccaggcc tcctacttgg tccatgtggc
     3121 tgagaacaac ccacctggaa cctccattgc tcaagtcagt gcttctgacc cggatttggg
     3181 atccaatggc ctcatttcct actccatcat agccagcgac ctagagccaa gggcactatc
     3241 ggctagcggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     3301 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     3361 gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     3421 gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     3481 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     3541 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3601 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     3661 ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     3721 caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca
     3781 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     3841 gagctaccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     3901 ggagttcgtg accgccgccg ggatcccacc ccctgctagc tccttcgtgt ccgtgaacca
     3961 ggacagtgga gtggtgttcg cgcagcgcgc cttcgaccac gagcagctgc gctccttcca
     4021 gctgacacta caggcgcggg accacggatc gcccacactc agtgccaatg tgagcatgcg
     4081 tgtgctggtg ggcgaccgca acgacaacgc gccacgcgtg ctgtatccca cacttgagcc
     4141 cgacggctct gcgctcttcg acatggtgcc gcgcgctgcc gagcccggat acctggtcac
     4201 caaggtggtg gctgtggatg cagactccgg acacaatgcc tggctatctt accacgtgct
     4261 gcaggccagc gatcccgggc tcttcagcct gggactgcgc acaggcgagg tgcgcacagc
     4321 gcgtgccctg ggagacaggg actcagcgcg gcagcgcctt ctggtcgctg tgcgtgatgg
     4381 tggacagcca ccgctctccg ctacagccac gctgcacctc atcttcgctg acagcctgca
     4441 ggaggtcctg ccagacctca gcgatgaccc tctaccatct gaccctcaga gtgagcttca
     4501 gttttacctg gtggtggcct tggccctgat ctcagtactc ttcctcctgt cggtgattct
     4561 ggctattgcc ctgcgtctga gacactcctc cagatcagct gcctggggct gctttcaacc
     4621 tggcctcagc tctaaccttg cgactggagt tctccccaat tacaatgagg ggactttacc
     4681 ttattcctac aatgtgtgta ttgcctcaca gtcagctaag acagagttta attttctcaa
     4741 tgtgactccg gaagttgctc ctcaggacct cctctgcggc gacgactctt gggtaccagg
     4801 tactcttgga gacactgacg ttccgtttgt ctcggattct atctcaaagc aagccccgcc
     4861 caacactgac tggcgtttct ctcaagccca gagacccggc acgagcggat cccaaaatgg
     4921 tgatgaaact ggcacctggc ccaacaacca gtttgataca gagatgctgc aagccatgat
     4981 cttggcctct gccagcgaag ctgctgatgg gagctctacc ctgggagggg gcgctggcac
     5041 catgggtctg agcgctcgat acggacccca gtttaccctg cagcacgtgc ctgactaccg
     5101 ccagaacgtg tacatccctg gcagcaatgc cacgctgacc aatgccgctg gcaaacgaga
     5161 tggcaaggct ccagcaggtg gtaatggcaa caagaagaaa tcgggcaaga aagagaagaa
     5221 gggcgttgcc atgccaggtg ccgaagatga tgtggtgtga gaattcactc ctcaggtgca
     5281 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     5341 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     5401 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     5461 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     5521 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     5581 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     5641 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     5701 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     5761 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     5821 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5881 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     5941 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca
     6001 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     6061 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     6121 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     6181 aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6241 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6301 aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg
     6361 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6421 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6481 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6541 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6601 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6661 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6721 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6781 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6841 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6901 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6961 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7021 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7081 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7141 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7201 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7261 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7321 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7381 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7441 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7501 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7561 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7621 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7681 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7741 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7801 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7861 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7921 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7981 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8041 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8101 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8161 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8221 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8281 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tg
//
