LOCUS       sequence-417742-       12260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for stable mammalian gene editing with C-terminally StayGold 
            tagged human Prom1s1 (WT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12260)
  AUTHORS   Bell TA, Luce BE, Hakim P, Ananda VY, Dardari H, Nguyen TH, 
            Monshizadeh A, Chao LH
  TITLE     Prominin 1 and Tweety Homology 1 both induce extracellular vesicle 
            formation.
  JOURNAL   Elife. 2024 Aug 13;13:e100061. doi: 10.7554/eLife.100061.
  PUBMED    39168849
REFERENCE   2  (bases 1 to 12260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-08-13"; volume: "13:e100061. doi"; pages: " 
            10.7554/eLife.100061"
COMMENT     Sequence Label: AAVS-HsProm1s1-StayGold
FEATURES             Location/Qualifiers
     source          1..12260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     215..234
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      576..1164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1065..1084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1318..1335
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1452..1473
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1488..1493,1494..1511,1512..1518)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1526..1542
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1550..1566
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1587..1605
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1644..2447
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    2454..2479
                     /label=SA
                     /note="splice acceptor site"
     CDS             2503..2556
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3029..3046)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3035..3259
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    3327..3359
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        3384..3595
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3608..3876
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      3904..3913
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6484..6504
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             6517..7167
                     /codon_start=1
                     /product="bright and highly photostable dimeric green 
                     fluorescent protein (Hirano et al., 2022)"
                     /label=StayGold
                     /note="engineered variant of a GFP from Cytaeis uchidae"
                     /translation="MASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKGKYVCTSGKL
                     PMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGSIHAKHQHFM
                     KNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVQHIPRDDGVECPVTLLYPLLSDKS
                     KCVEAHQNTICKPLHNQPAPDVPYHWIRKQYTQSKDDTEERDHICQSETLEAHL"
     misc_feature    7228..7816
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7281..7301)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7699..7710)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7848..8324
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(8047..8066)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(8434..8453)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8499..8554
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8553..8572)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8915..8931)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8939..8955
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8963..8969,8970..8987,8988..8993))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9008..9029
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    9103..9939
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(9973..9992)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9974..9992)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9999..10016)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9999..10015)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10008..10030)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             10153..10455
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10334..10353
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(10858..10877)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11472..11491
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(11852..12260,1..383,384..452))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
       61 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
      121 agatgctttt ctgtgactgg tgaatactca accaagtcat tctgagaata gtgtatgcgg
      181 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
      241 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
      301 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
      361 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
      421 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
      481 atttatcagg gttattgtct catgaccaaa atcccttaac gtgagttttc gttccactga
      541 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      601 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
      661 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
      721 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
      781 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
      841 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
      901 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
      961 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1021 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1081 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     1141 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     1201 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     1261 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     1321 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     1381 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     1441 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     1501 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     1561 tatgaccatg attacgccaa gctcagaatt aaccctcact aaagggacta gctagtcctg
     1621 caggtttaaa cgaattcgcc ctttgctttc tctgaccagc attctctccc ctgggcctgt
     1681 gccgctttct gtctgcagct tgtggcctgg gtcacctcta cggctggccc agatccttcc
     1741 ctgccgcctc cttcaggttc cgtcttcctc cactccctct tccccttgct ctctgctgtg
     1801 ttgctgccca aggatgctct ttccggagca cttccttctc ggcgctgcac cacgtgatgt
     1861 cctctgagcg gatcctcccc gtgtctgggt cctctccggg catctctcct ccctcaccca
     1921 accccatgcc gtcttcactc gctgggttcc cttttccttc tccttctggg gcctgtgcca
     1981 tctctcgttt cttaggatgg ccttctccga cggatgtctc ccttgcgtcc cgcctcccct
     2041 tcttgtaggc ctgcatcatc accgtttttc tggacaaccc caaagtaccc cgtctccctg
     2101 gctttagcca cctctccatc ctcttgcttt ctttgcctgg acaccccgtt ctcctgtgga
     2161 ttcgggtcac ctctcactcc tttcatttgg gcagctcccc tacccccctt acctctctag
     2221 tctgtgctag ctcttccagc cccctgtcat ggcatcttcc aggggtccga gagctcagct
     2281 agtcttcttc ctccaacccg ggcccctatg tccacttcag gacagcatgt ttgctgcctc
     2341 cagggatcct gtgtccccga gctgggacca ccttatattc ccagggccgg ttaatgtggc
     2401 tctggttctg ggtactttta tctgtcccct ccaccccaca gtggggcaag cttctgacct
     2461 cttctcttcc tcccacaggg cctcgagaga tctggcagcg gagagggcag aggaagtctt
     2521 ctaacatgcg gtgacgtgga ggagaatccc ggccctagga ccggtatgaa aacatttaac
     2581 atttctcaac aagatctaga attagtagaa gtagcgacag agaagattac aatgctttat
     2641 gaggataata aacatcatgt gggagcggca attcgtacga aaacaggaga aatcatttcg
     2701 gcagtacata ttgaagcgta tataggacga gtaactgttt gtgcagaagc cattgcgatt
     2761 ggtagtgcag tttcgaatgg acaaaaggat tttgacacga ttgtagctgt tagacaccct
     2821 tattctgacg aagtagatag aagtattcga gtggtaagtc cttgtggtat gtgtagggag
     2881 ttgatttcag actatgcacc agattgtttt gtgttaatag aaatgaatgg caagttagtc
     2941 aaaactacga ttgaagaact cattccactc aaatataccc gaaattaatg agaattcggg
     3001 gatcaattct ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     3061 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3121 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3181 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3241 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc gactagagct
     3301 tgcggaaccc ttaatggtac cgtctctgcc agggcgtgcc cttgggctcc ccgggcgcga
     3361 ctagtgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     3421 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga
     3481 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     3541 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa
     3601 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc
     3661 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc
     3721 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag
     3781 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt
     3841 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgcc
     3901 gccgccacca tggccctcgt actcggctcc ctgttgctgc tggggctgtg cgggaactcc
     3961 ttttcaggag ggcagccttc atccacagat gctcctaagg cttggaatta tgaattgcct
     4021 gcaacaaatt atgagaccca agactcccat aaagctggac ccattggcat tctctttgaa
     4081 ctagtgcata tctttctcta tgtggtacag ccgcgtgatt tcccagaaga tactttgaga
     4141 aaattcttac agaaggcata tgaatccaaa attgattatg acaagattgt ctactatgaa
     4201 gcagggatta ttctatgctg tgtcctgggg ctgctgttta ttattctgat gcctctggtg
     4261 gggtatttct tttgtatgtg tcgttgctgt aacaaatgtg gtggagaaat gcaccagcga
     4321 cagaaggaaa atgggccctt cctgaggaaa tgctttgcaa tctccctgtt ggtgatttgt
     4381 ataataataa gcattggcat cttctatggt tttgtggcaa atcaccaggt aagaacccgg
     4441 atcaaaagga gtcggaaact ggcagatagc aatttcaagg acttgcgaac tctcttgaat
     4501 gaaactccag agcaaatcaa atatatattg gcccagtaca acactaccaa ggacaaggcg
     4561 ttcacagatc tgaacagtat caattcagtg ctaggaggcg gaattcttga ccgactgaga
     4621 cccaacatca tccctgttct tgatgagatt aagtccatgg caacagcgat caaggagacc
     4681 aaagaggcgt tggagaacat gaacagcacc ttgaagagct tgcaccaaca aagtacacag
     4741 cttagcagca gtctgaccag cgtgaaaact agcctgcggt catctctcaa tgaccctctg
     4801 tgcttggtgc atccatcaag tgaaacctgc aacagcatca gattgtctct aagccagctg
     4861 aatagcaacc ctgaactgag gcagcttcca cccgtggatg cagaacttga caacgttaat
     4921 aacgttctta ggacagattt ggatggcctg gtccaacagg gctatcaatc ccttaatgat
     4981 atacctgaca gagtacaacg ccaaaccacg actgtcgtag caggtatcaa aagggtcttg
     5041 aattccattg gttcagatat cgacaatgta actcagcgtc ttcctattca ggatatactc
     5101 tcagcattct ctgtttatgt taataacact gaaagttaca tccacagaaa tttacctaca
     5161 ttggaagagt atgattcata ctggtggctg ggtggcctgg tcatctgctc tctgctgacc
     5221 ctcatcgtga ttttttacta cctgggctta ctgtgtggcg tgtgcggcta tgacaggcat
     5281 gccaccccga ccacccgagg ctgtgtctcc aacaccggag gcgtcttcct catggttgga
     5341 gttggattaa gtttcctctt ttgctggata ttgatgatca ttgtggttct tacctttgtc
     5401 tttggtgcaa atgtggaaaa actgatctgt gaaccttaca cgagcaagga attattccgg
     5461 gttttggata caccctactt actaaatgaa gactgggaat actatctctc tgggaagcta
     5521 tttaataaat caaaaatgaa gctcactttt gaacaagttt acagtgactg caaaaaaaat
     5581 agaggcactt acggcactct tcacctgcag aacagcttca atatcagtga acatctcaac
     5641 attaatgagc atactggaag cataagcagt gaattggaaa gtctgaaggt aaatcttaat
     5701 atctttctgt tgggtgcagc aggaagaaaa aaccttcagg attttgctgc ttgtggaata
     5761 gacagaatga attatgacag ctacttggct cagactggta aatcccccgc aggagtgaat
     5821 cttttatcat ttgcatatga tctagaagca aaagcaaaca gtttgccccc aggaaatttg
     5881 aggaactccc tgaaaagaga tgcacaaact attaaaacaa ttcaccagca acgagtcctt
     5941 cctatagaac aatcactgag cactctatac caaagcgtca agatacttca acgcacaggg
     6001 aatggattgt tggagagagt aactaggatt ctagcttctc tggattttgc tcagaacttc
     6061 atcacaaaca atacttcctc tgttattatt gaggaaacta agaagtatgg gagaacaata
     6121 ataggatatt ttgaacatta tctgcagtgg atcgagttct ctatcagtga gaaagtggca
     6181 tcgtgcaaac ctgtggccac cgctctagat actgctgttg atgtctttct gtgtagctac
     6241 attatcgacc ccttgaattt gttttggttt ggcataggaa aagctactgt atttttactt
     6301 ccggctctaa tttttgcggt aaaactggct aagtactatc gtcgaatgga ttcggaggac
     6361 gtgtacgatg atgttgaaac tatacccatg aaaaatatgg aaaatggtaa taatggttat
     6421 cataaagatc atgtatatgg tattcacaat cctgttatga caagcccatc acaacatctc
     6481 gaggagaatt tgtactttca aggcggtgga ggagggatgg cctcaacacc cttcaagttc
     6541 caattgaagg gcacaatcaa cggtaaatct ttcactgtgg agggcgaggg ggagggcaac
     6601 agccatgaag ggtctcacaa aggcaaatac gtgtgcacga gcgggaagct gcccatgtca
     6661 tgggccgctc tggggacaag ctttgggtat ggaatgaaat actacactaa gtatcctagt
     6721 gggcttaaga actggtttca cgaggtgatg cctgaaggct tcacatacga taggcatatt
     6781 cagtacaaag gcgacggaag tatccatgca aagcaccaac attttatgaa gaatgggacc
     6841 tatcataaca tcgtggaatt tactggccag gactttaagg aaaacagccc cgtactgaca
     6901 ggagacatga atgtcagtct ccctaacgag gtgcagcata tcccccgaga cgacggcgtg
     6961 gagtgtcccg tgactctcct gtacccgctc ctctccgata agtccaagtg tgtggaggcc
     7021 caccaaaata ctatctgtaa acctctgcat aatcagcccg ctcccgatgt accataccat
     7081 tggattcgga agcagtacac ccagagcaag gatgacaccg aagagcggga tcatatatgt
     7141 caaagcgaaa ccctcgaggc ccacctctaa cctgcaggcg gatccgcgtt taaactcgag
     7201 gttaattaag atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     7261 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7321 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7381 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7441 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7501 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7561 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7621 aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct gcgcgggacg
     7681 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7741 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7801 tgggccgcct ccccgcatcg ataccgagcg ctgctcgaga gatctacggg tggcatccct
     7861 gtgacccctc cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc
     7921 ttgtcctaat aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta
     7981 tggggtggag gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct
     8041 gcggggtcta ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct
     8101 ccgcctcctg ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg
     8161 catgcatgac caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat
     8221 tggccaggct ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat
     8281 tgctgggatt acaggcgtga accactgctc ccttccctgt ccttctgatt ttgtaggtaa
     8341 ccaggcgcgt gaattcactc ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt
     8401 ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca aaaattatgg
     8461 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     8521 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     8581 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     8641 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     8701 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     8761 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     8821 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     8881 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     8941 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     9001 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     9061 cgggaaacct gtcgtgccag cggatcgaca gtactaagct ttactaggga caggattggt
     9121 gacagaaaag ccccatcctt aggcctcctc cttcctagtc tcctgatatt gggtctaacc
     9181 cccacctcct gttaggcaga ttccttatct ggtgacacac ccccatttcc tggagccatc
     9241 tctctccttg ccagaacctc taaggtttgc ttacgatgga gccagagagg atcctgggag
     9301 ggagagcttg gcagggggtg ggagggaagg gggggatgcg tgacctgccc ggttctcagt
     9361 ggccaccctg cgctaccctc tcccagaacc tgagctgctc tgacgcggct gtctggtgcg
     9421 tttcactgat cctggtgctg cagcttcctt acacttccca agaggagaag cagtttggaa
     9481 aaacaaaatc agaataagtt ggtcctgagt tctaactttg gctcttcacc tttctagtcc
     9541 ccaatttata ttgttcctcc gtgcgtcagt tttacctgtg agataaggcc agtagccagc
     9601 cccgtcctgg cagggctgtg gtgaggaggg gggtgtccgt gtggaaaact ccctttgtga
     9661 gaatggtgcg tcctaggtgt tcaccaggtc gtggccgcct ctactccctt tctctttctc
     9721 catccttctt tccttaaaga gtccccagtg ctatctggga catattcctc cgcccagagc
     9781 agggtcccgc ttccctaagg ccctgctctg ggcttctggg tttgagtcct tggcaagccc
     9841 aggagaggcg ctcaggcttc cctgtccccc ttcctcgtcc accatctcat gcccctggct
     9901 ctcctgcccc ttccctacag gggttcctgg ctctgctcta agggcgaatt cgcggccgct
     9961 aaattcaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt
    10021 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    10081 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    10141 ctatacgtac ggcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt
    10201 gtggatgtac agagtgatat tattgacacg ccggggcgac ggatggtgat ccccctggcc
    10261 agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg
    10321 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg
    10381 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg
    10441 ttctggggaa tataaatgtc aggcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    10501 ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg
    10561 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt
    10621 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct
    10681 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg
    10741 ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt
    10801 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
    10861 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
    10921 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
    10981 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
    11041 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
    11101 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
    11161 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
    11221 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
    11281 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
    11341 gacggcgagg atctcgtcgt gacccatgca tggcgatgcc tgcttgccga atatcatggt
    11401 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
    11461 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
    11521 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
    11581 ccttcttgac gagttcttct gaattattaa cgcttacaat ttcctgatgc ggtattttct
    11641 ccttacgcat ctgtgcggta tttcacaccg catcaggtgg cacttttcgg ggaaatgtgc
    11701 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagat
    11761 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    11821 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    11881 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    11941 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    12001 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    12061 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    12121 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    12181 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    12241 ttacatgatc ccccatgttg
//
