LOCUS       sequence-417745-       11626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus backbone expressing a doxycycline-inducible mitochondrial
            localized ApaLI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Fu Y, Sacco O, DeBitetto E, Kanshin E, Ueberheide B, Sfeir A
  TITLE     Mitochondrial DNA breaks activate an integrated stress response to 
            reestablish homeostasis.
  JOURNAL   Mol Cell. 2023 Oct 8:S1097-2765(23)00753-0. doi: 
            10.1016/j.molcel.2023.09.026.
  PUBMED    37832546
REFERENCE   2  (bases 1 to 11626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2023 Oct 8:S1097-2765(23)00753-0. doi: 
            10.1016/j.molcel.2023.09.026."
COMMENT     Sequence Label: TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro
FEATURES             Location/Qualifiers
     source          1..11626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    69..302
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             487..531
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    829..946
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1044..1358
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1052..1070
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1088..1106
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1123..1141
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1159..1177
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1195..1213
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1230..1248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1266..1284
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1331..1355
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1373..1447
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             2606..2629
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2654..2707
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2726..3421
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="ELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVE
                     GGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQ
                     DTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLV
                     GGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDL
                     PSKLGHKLN"
     promoter        3444..3655
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3677..4273
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3677..3696)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4173..4193
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4283..4339
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(4340..4957,4958..4963,4964..4999,5000..5002,5003..5038,
                     5039..5041,5042..5077,5078..5086)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5107..5695
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5160..5180)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5578..5589)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5767..6000
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6026..6043)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6032..6256
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6302..6730
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6389..6408)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6599..6620
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6739..6759)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6744..7073
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6924..7059
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6986..7005
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7121..7168
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7187..7561
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7691..7812
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7728..7747)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7782..7801
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7861..7877)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7861..7877)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7874..7896)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7885..7901
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7909..7915,7916..7933,7934..7939))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7954..7975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8092..8109)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8263..8851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8343..8362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9022..9813,9814..9882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9645..9664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9883..9987)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10062..10081)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10253..10632
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10634..10832
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             10850..11030
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11077..11202
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
       61 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
      121 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
      181 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
      241 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
      301 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
      361 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
      421 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
      481 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
      541 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
      601 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
      661 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
      721 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
      781 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
      841 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
      901 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
      961 ggacagcaga gatccagttt ggttaattaa ggtaccgagg gcctatttct tttgaattca
     1021 ctttggccgc gaatcgatat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
     1081 agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc agtgatagag
     1141 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
     1201 tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac gtatgtcgag
     1261 tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct
     1321 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaataccg gtatgtccgt
     1381 cctgacgccg ctgctgctgc ggggcttgac aggctcggcc cggcggctcc cagtgccgcg
     1441 cgccaagatc cattcgttgc cgccggaggg gaagcttatg accacacgac agcgcttatc
     1501 tgctgagaga tctcagcagc ttacccgtct gctcactatc actaaaaccg ctaacatgcg
     1561 ggccttaatg gaggctagcg aactcgctaa agtaattgcc ctggtggcgg ttgacatagg
     1621 taagtcagac gagatggcca gagctttccc cgtgctgtgg ccaaaaatca gtcctcagca
     1681 agaatattac gcaacagcgg ttgactggtt caccaacccc gatgaaaccg tcacctcttt
     1741 cgatgtggtg gatatgctag atgccggcac ttccctggac caagacttca tgacttatct
     1801 gaaatgtcta actgaactgc acaagcgacg gagaaaatac ggcctgatcc tgcagaggca
     1861 gcctctacca acgatggtgc aggtttcgcc ccgagcactg atggaatacg gacctgactt
     1921 tccgcccgag gcactggcta gctggcttac ctggcggaag tttttttacg accttgacaa
     1981 caggtctgcc caggaaacag ggtacttgtt cgaacccatt ctggccgccg ccatcggagg
     2041 cgaggcaaaa tctgccagag agcgtgtcgt aagacgcacc gacgatccaa cgaagggcag
     2101 gcaggtggat tgctggaagg tgctccctga tggtacgcct ctggcttatg aattgaaact
     2161 tcgggtgaca attgcagcga gcgggcaagg cagatttgga gaggagctga gcttcgctcg
     2221 ggactgcagc agctccggcg ctaagccgat cctcgtggtc ctggatccaa cagaaaatga
     2281 caagctcact ggattgcaag ccgcataccg cgaggttgga ggcgccgcat atgtggggga
     2341 tgccgcctgg gctcacttag aggacgaggc tggtgctacc atggccagtt ttatagagcg
     2401 ctatgtcagg gtgccagtag catcagtcag ttccttcgaa agggtcattg agggagatgc
     2461 cacaaagcgc tccttgatcc tgcaggacct ccaggcccga ctcgatggta atgagcttac
     2521 aatctccctg ggaggccatc agcggttggt ggaaaggcat gaggaccaga gtctcgcagc
     2581 ggacggcgat gacgattcag agctcgatta caaagacgat gacgataagg gcgcgcctgg
     2641 atccggcagt ggagagggca gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc
     2701 tggcccaatg gtgagcaagg gtgaggagct gattaaggag aacatgcaca tgaagctgta
     2761 catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc
     2821 ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc
     2881 cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca
     2941 gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac
     3001 cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg
     3061 cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca
     3121 gaagaaaaca ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct
     3181 ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacgc
     3241 caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta
     3301 tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca
     3361 cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaattg
     3421 accattcgat tgggctagca tccgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     3481 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga
     3541 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     3601 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagactgc
     3661 gatcgcaatg tacagtatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     3721 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     3781 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     3841 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     3901 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     3961 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     4021 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca
     4081 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     4141 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     4201 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     4261 caagcccggt gccggttccg gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt
     4321 cgaagagaat cctggaccga tgtctagact ggacaagagc aaagtcataa acggagctct
     4381 ggaattactc aatggtgtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
     4441 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
     4501 cctgccaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
     4561 atggcaagac tttctgcgga acaacgccaa gtcataccgc tgtgctctcc tctcacatcg
     4621 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
     4681 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
     4741 gtccgccgtg ggccacttta cactgggctg cgtattggag gaacaggagc atcaagtagc
     4801 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
     4861 tgagctgttc gaccggcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
     4921 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccgaccgacg cccttgacga
     4981 ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc
     5041 tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaactcg agcgcgttaa
     5101 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5161 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5221 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5281 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5341 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5401 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5461 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     5521 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     5581 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     5641 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     5701 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5761 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     5821 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5881 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5941 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6001 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     6061 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     6121 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     6181 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     6241 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     6301 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6361 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6421 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6481 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6541 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6601 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6661 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6721 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     6781 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     6841 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6901 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6961 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     7021 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     7081 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     7141 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     7201 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     7261 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     7321 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     7381 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     7441 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     7501 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     7561 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     7621 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     7681 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7741 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7801 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     7861 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7921 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7981 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     8041 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     8101 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8161 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8221 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8281 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8341 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8401 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8461 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8521 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8581 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8641 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8701 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8761 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8821 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8881 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8941 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     9001 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9061 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9121 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9181 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9241 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9301 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9361 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9421 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9481 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9541 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9601 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9661 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9721 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9781 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9841 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9901 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9961 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
    10021 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
    10081 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
    10141 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
    10201 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
    10261 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
    10321 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
    10381 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
    10441 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
    10501 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
    10561 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
    10621 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
    10681 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
    10741 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
    10801 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
    10861 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
    10921 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    10981 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
    11041 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
    11101 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
    11161 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
    11221 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
    11281 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
    11341 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
    11401 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    11461 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    11521 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
    11581 caattggaga agtgaattat ataaatataa agtagtaaaa attgaa
//
