LOCUS       sequence-417748-        3753 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mutant E3-DART  ligase in domestication vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Huang L, Rojas-Pierce M
  TITLE     Rapid depletion of target proteins in plants by an inducible protein
            degradation system.
  JOURNAL   Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072.
  PUBMED    38446628
REFERENCE   2  (bases 1 to 3753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2024 Mar 6:koae072. doi: 10.1093/plcell/koae072."
COMMENT     Sequence Label: pUPD2-HA-LRR-NELC492A-C (B3-B4)
FEATURES             Location/Qualifiers
     source          1..3753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             25..51
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             173..184
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     terminator      1710..1767
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1791..1808)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1962..2550)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2042..2061)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2733..2827)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2848..3507)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3422..3441
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3669..3687
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3691..3734)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(3737..3753,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg aatgtaccca tacgatgttc cagattacgc tgagtatgat
       61 gctgtctggt caaaatggga aagggatgca ccagcaggag agtcacctgg aagagcagca
      121 gtggttcagg aaatgcgtga ttgccttaat aacggaaatc cagtgcttaa cgtgggagct
      181 tcaggtctta ccacccttcc agaccgtctt ccaccgcata ttacaacact tgttattcca
      241 gataataatc tcacctcttt gcctgagttg ccagaaggac ttagagagct tgaggtgtct
      301 ggaaaccttc agcttacatc tcttccatct cttccgcagg gtcttcagaa gttgtgggcc
      361 tataacaatt ggcttgcctc tcttccgact ttgccgccag gacttgggga tcttgctgtc
      421 tctaataacc agcttacctc tcttccggag atgccgccag cacttagaga gcttagggtg
      481 tctggtaaca accttacctc tcttccggct cttccgtcag gacttcagaa gctttgggca
      541 tataacaata gattgacctc tttgccagag atgtcaccag gtcttcagga acttgatgtc
      601 tctcataatc agcttaccag attgccgcaa tctctcactg gtctttcttc tgctgcaaga
      661 gtttatcttg acgggaatcc actttctgtt agaactcttc aggctcttag agacatcatt
      721 ggacattcag gaatcaggat acacttcgat atggctgggc cttctgtccc tagagaagcc
      781 agagcacttc accttgctgt cgctgactgg cttacttctg caagagaggg ggaagctgcc
      841 caggcagaca gatggcaggc tttcggactt gaagataacg ccgccgcctt ctctcttgtc
      901 cttgacagac ttcgtgagac tgaaaacttc aaaaaagacg ctggatttaa ggcacagata
      961 tcatcttggc ttacacaact tgctgaagat gctgctctta gagcaaaaac ctttgccatg
     1021 gcaacagagg caacatcaac cgctgaggac agagtcacac atgcccttca ccagatgaat
     1081 aacgttcaac ttgttcataa tgcagaaaaa ggggaatacg acaacaatct ccaggggctt
     1141 gtttctactg ggcgtgagat gttcagactt gcaacacttg aacagattgc cagagaaaaa
     1201 gccggaacac ttgctcttgt cgatgacgtt gaggtctatc ttgctttcca gaataagctt
     1261 aaggaatcac ttgagcttac ctctgtgact tcagaaatgc gtttctttga cgtttctgga
     1321 gtgactgttt cagaccttca ggctgctgag cttcaggtga aaaccgctga aaactctggg
     1381 ttcagtaaat ggatacttca gtgggggccg cttcactctg tgcttgaaag aaaagtgccg
     1441 gaaagattta acgctcttcg tgaaaagcaa atatctgatt atgaagacac ttacagaaag
     1501 ctttatgacg aagtgcttaa atcttctggg cttgtcgacg ataccgatgc agaacgtact
     1561 atcggagtta gtgctatgga tagtgctaaa aaagaatttc ttgatggact tagagctctt
     1621 gtggatgagg tgcttggttc ttatcttaca gccagatggc gtcttaactt cgtgagacca
     1681 ctcatcgcga ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc
     1741 tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact
     1801 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1861 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc
     1981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2521 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2581 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2641 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2701 gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg
     2761 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     2821 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcgt cgcagtactg
     2881 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     2941 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     3001 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     3061 gggattggct gatacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     3121 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     3181 gtattcactc cagagtgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     3241 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
     3301 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     3361 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     3421 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     3481 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     3541 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     3601 gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     3661 cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
     3721 ctaaggatga tttctggaat tcgcggccgc ttc
//
