LOCUS       sequence-417753-        3729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CDS for E3-DART ligase in domestication vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Huang L, Rojas-Pierce M
  TITLE     Rapid depletion of target proteins in plants by an inducible protein
            degradation system.
  JOURNAL   Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072.
  PUBMED    38446628
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2024 Mar 6:koae072. doi: 10.1093/plcell/koae072."
COMMENT     Sequence Label: pUPD2-LRR-NEL (B4-B5)
FEATURES             Location/Qualifiers
     source          1..3729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             146..157
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     terminator      1686..1743
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1767..1784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1938..2526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2018..2037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2709..2803)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2824..3483)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3398..3417
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3645..3663
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3667..3710)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(3713..3729,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg agccgagtat gatgctgtct ggtcaaaatg ggaaagggat
       61 gcaccagcag gagagtcacc tggaagagca gcagtggttc aggaaatgcg tgattgcctt
      121 aataacggaa atccagtgct taacgtggga gcttcaggtc ttaccaccct tccagaccgt
      181 cttccaccgc atattacaac acttgttatt ccagataata atctcacctc tttgcctgag
      241 ttgccagaag gacttagaga gcttgaggtg tctggaaacc ttcagcttac atctcttcca
      301 tctcttccgc agggtcttca gaagttgtgg gcctataaca attggcttgc ctctcttccg
      361 actttgccgc caggacttgg ggatcttgct gtctctaata accagcttac ctctcttccg
      421 gagatgccgc cagcacttag agagcttagg gtgtctggta acaaccttac ctctcttccg
      481 gctcttccgt caggacttca gaagctttgg gcatataaca atagattgac ctctttgcca
      541 gagatgtcac caggtcttca ggaacttgat gtctctcata atcagcttac cagattgccg
      601 caatctctca ctggtctttc ttctgctgca agagtttatc ttgacgggaa tccactttct
      661 gttagaactc ttcaggctct tagagacatc attggacatt caggaatcag gatacacttc
      721 gatatggctg ggccttctgt ccctagagaa gccagagcac ttcaccttgc tgtcgctgac
      781 tggcttactt ctgcaagaga gggggaagct gcccaggcag acagatggca ggctttcgga
      841 cttgaagata acgccgccgc cttctctctt gtccttgaca gacttcgtga gactgaaaac
      901 ttcaaaaaag acgctggatt taaggcacag atatcatctt ggcttacaca acttgctgaa
      961 gatgctgctc ttagagcaaa aacctttgcc atggcaacag aggcaacatc aacctgcgag
     1021 gacagagtca cacatgccct tcaccagatg aataacgttc aacttgttca taatgcagaa
     1081 aaaggggaat acgacaacaa tctccagggg cttgtttcta ctgggcgtga gatgttcaga
     1141 cttgcaacac ttgaacagat tgccagagaa aaagccggaa cacttgctct tgtcgatgac
     1201 gttgaggtct atcttgcttt ccagaataag cttaaggaat cacttgagct tacctctgtg
     1261 acttcagaaa tgcgtttctt tgacgtttct ggagtgactg tttcagacct tcaggctgct
     1321 gagcttcagg tgaaaaccgc tgaaaactct gggttcagta aatggatact tcagtggggg
     1381 ccgcttcact ctgtgcttga aagaaaagtg ccggaaagat ttaacgctct tcgtgaaaag
     1441 caaatatctg attatgaaga cacttacaga aagctttatg acgaagtgct taaatcttct
     1501 gggcttgtcg acgataccga tgcagaacgt actatcggag ttagtgctat ggatagtgct
     1561 aaaaaagaat ttcttgatgg acttagagct cttgtggatg aggtgcttgg ttcttatctt
     1621 acagccagat ggcgtcttaa ctgagctttg agaccactca tcgcgactag tagcggccgc
     1681 tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa
     1741 agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1801 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1861 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1921 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
     1981 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2041 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2101 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2161 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2221 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2281 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2341 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2401 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2461 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2521 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2581 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2641 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagctcg
     2701 aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc
     2761 agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc
     2821 aaattacgcc ccgccctgcc actcgtcgca gtactgttgt aattcattaa gcattctgcc
     2881 gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt
     2941 gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt
     3001 ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgata cgaaaaacat
     3061 attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg
     3121 cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga gtgatgaaaa
     3181 cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag
     3241 ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat
     3301 gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt
     3361 aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa
     3421 atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc
     3481 cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga
     3541 tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact cgagtgccac
     3601 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3661 ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggaattcgc
     3721 ggccgcttc
//
