LOCUS       sequence-417754-       11374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI in
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11374)
  AUTHORS   Rahikainen R, Vester SK, Turkki P, Janosko CP, Deiters A, Hytonen 
            VP, Howarth M
  TITLE     Visible Light-Induced Specific Protein Reaction Delineates Early 
            Stages of Cell Adhesion.
  JOURNAL   J Am Chem Soc. 2023 Nov 15;145(45):24459-24465. doi: 
            10.1021/jacs.3c07827. Epub 2023 Oct 31.
  PUBMED    38104267
REFERENCE   2  (bases 1 to 11374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.3c07827"; journalName: "J Am Chem Soc"; date: 
            "2023-11-15- 15"; volume: "145"; issue: "45"; pages: "24459-24465"
COMMENT     Sequence Label: SpyCatcher003(K31TAG)-Talin rod-mScarletI
FEATURES             Location/Qualifiers
     source          1..11374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        83..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      628..637
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8184..8305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8221..8240)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8275..8294
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8312..8767)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8449..8470)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8661..8680
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8794..8898
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8900..9257
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8900..9091
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8923..8943)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9108..9243
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9292..10086
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9292..10086
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9346..9365)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9956..9975
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10274..10293)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10318..10365
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10694..11282
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11183..11202
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
      601 accgtcagat ccgctagcgc taccggtgcc accatggtga ccacactgtc cggactgtct
      661 ggagagcagg gaccatccgg cgacatgacc acagaggagg attctgccac acacatcaag
      721 ttcagctaga gggacgagga cggaagagag ctggcaggag caaccatgga gctgagggat
      781 agctccggca agaccatcag cacatggatc tccgacggcc acgtgaagga tttctacctg
      841 tatcccggca agtacacctt tgtggagaca gcagcaccag acggatatga ggtggcaacc
      901 cctatcgagt ttacagtgaa cgaggacgga caggtgaccg tggatggaga ggcaacagag
      961 ggcgatgcac acaccggtgg atccggccag cagtacaacc gagtggggaa agtggagcac
     1021 ggctctgtgg ctctgccagc catcatgcgc tctggagcct ctggtcctga gaatttccag
     1081 gtggggagca tgccacctgc ccagcagcag atcaccagtg gccagatgca ccgaggacac
     1141 atgccacctc tgacttcagc ccagcaggcg ctcactggaa ccattaactc cagcatgcag
     1201 gctgtgcagg ctgcccaggc cactctggat gactttgaga ctctaccccc tcttggccag
     1261 gatgctgcct ccaaagcttg gcgtaagaac aagatggatg aatcaaagca tgagatccac
     1321 tcccaggtag atgccatcac agctggcact gcctctgtgg taaacctgac agcaggagat
     1381 cctgcagaga cagactatac agcagtgggc tgtgcagtca ccaccatctc ttccaacctg
     1441 acggagatgt cccgtggggt gaagctattg gctgccctgc tggaggatga aggcggcaat
     1501 ggccggcccc ttctgcaagc agcaaagggc cttgcagggg ctgtgtcgga actgcttcgc
     1561 agtgctcagc ccgccagcgc tgagcctcgt cagaacctgc tgcaggcagc cgggaacgtg
     1621 ggccaggcca gtggggagct gttgcagcaa attggggaaa gtgacactga cccccacttc
     1681 caggatgttc taatgcagct agcaaacgca gtggcaagtg ctgcagccgc cctggtcctc
     1741 aaggccaaga gtgtggccca gcgcacagag gattccgggc ttcagaccca agtgattgct
     1801 gcagctacgc aatgtgctct gtccacttcc cagctggtgg cctgtaccaa ggtggtagca
     1861 cctacaatca gctcacctgt gtgccaagaa cagctagtcg aggctgggcg actggtggcc
     1921 aaagctgtgg agggctgtgt gtctgcctcg caagcagcta cagaagatgg acagctgctt
     1981 cgaggggtag gagcagcagc cacggctgtc acccaggccc tcaatgagct gctgcagcac
     2041 gtgaaggccc acgccaccgg agctgggcct gccggtcggt atgaccaagc cactgatacc
     2101 atcctcaccg tcactgaaaa catcttcagc tccatgggtg atgctgggga gatggtgcgc
     2161 caggcccgca tcctggccca agccacctca gacctggtca atgctatcaa ggccgatgct
     2221 gagggggaga gtgatctgga gaactctaga aagctcctga gtgctgccaa gatcctcgct
     2281 gatgccaccg ccaagatggt ggaggcggcc aagggagcag ccgcccaccc tgacagtgag
     2341 gaacagcagc agcgactgcg tgaagcagct gaggggcttc gcatggccac caatgcagct
     2401 gcgcagaacg ccatcaagaa gaagttggtg cagcgcctgg agcatgcagc caagcaagct
     2461 gcagcctctg caacacagac cattgctgca gcccaacatg cagcctctgc ccccaaggcc
     2521 tctgcgggcc cccagcccct actggtgcag agctgtaagg ccgtggcaga gcagattccg
     2581 ttgctggtgc agggtgtccg aggaagtcaa gctcagcctg acagccctag tgctcagctc
     2641 gccctcattg ctgccagcca gagcttcctg cagccaggtg ggaagatggt ggcagcggca
     2701 aaagcctcag tgccaacaat tcaggaccag gcttcagcga tgcagctgag tcagtgcgca
     2761 aagaacctag gcactgccct ggccgagctg cgcactgctg ctcagaaggc tcaggaggca
     2821 tgtggacctt tggagatgga ttctgcactg agtgttgtac aaaatctaga gaaagaccta
     2881 caggaaataa aagcagcagc tcgagatggc aagcttaagc ccttacctgg ggaaacaatg
     2941 gagaagtgta cccaagatct tggcaacagc accaaagcgg tgagctctgc catcgccaag
     3001 ctgctggggg agattgccca gggcaacgag aactatgcag gtattgcagc tcgggatgta
     3061 gctggtggac taaggtcact agcccaggct gcgcggggtg tggctgcgct gacatcagat
     3121 cctgcagtgc aggccattgt gcttgacaca gccagcgacg ttctggacaa ggccagcagc
     3181 ctcattgaag aggcaaaaaa agcatctgga cacccagggg acccagaaag ccagcaaagg
     3241 cttgctcagg tagctaaagc agtgacccaa gccctgaacc gctgtgtcag ctgcctgcct
     3301 ggccaaagag acgtggataa tgccctacga gccgttggag atgccagcaa gcgactcctg
     3361 agtgacttgc ttcctccaag cacggggaca tttcaagaag cacagagccg attgaatgaa
     3421 gctgcagctg ggttaaatca ggccgccaca gaactggtgc aagcctcccg aggaactcct
     3481 caggacctgg ctcgggcctc cggccgattt ggacaggact tcagcacctt cctggaagct
     3541 ggtgtggaga tggccggaca ggccccgagc caggaggacc gagcccaggt ggtgtccaac
     3601 ctgaagggca tatccatgtc ttcaagcaaa cttctccttg ccgctaaggc cttgtccaca
     3661 gaccctgctt ctcccaacct caagagtcag ctggctgcgg ctgcccgggc agtgacggac
     3721 agcatcaacc agctcatcac catgtgcacc cagcaggcac ctggccagaa ggagtgtgac
     3781 aatgcacttc ggcagctaga gacagtccga gaactcctgg agaatccagt ccagcccatc
     3841 aacgacatgt cctacttcgg ttgcttggac agtgtcatgg agaactccaa ggtcctaggt
     3901 gaggccatga ctggcatctc ccaaaatgcc aagaatggaa atctgccgga gtttggagat
     3961 gccattgcca cagcctccaa ggctctctgt ggcttcacgg aggcagccgc acaggcagca
     4021 tatctagttg gtgtctctga ccccaacagc caagctggac agcaaggact ggtggaaccc
     4081 acacagtttg cccgtgcaaa ccaggcaatt cagatggcct gtcagagtct gggggagcct
     4141 ggctgtaccc aggcccaggt gttatctgca gccactattg tagccaaaca cacatctgca
     4201 ttgtgtaaca gctgtcgcct ggcttccgct aggactgcca atcccactgc caagcgccag
     4261 tttgtacagt cagccaagga ggtggccaac agtacagcca atcttgtcaa gaccatcaag
     4321 gcactagatg gggacttcac agaagagaac cgtgcccagt gccgagcagc cacagcccct
     4381 ctgctggaag ctgtggataa cctgagtgcc tttgcctcca accctgagtt ctccagcgtc
     4441 cctgcccaga tcagccctga gggcagagca gccatggagc ccattgtaat ctctgctaag
     4501 acaatgttgg agagtgctgg agggctcatc cagacagctc gggcgttagc agtcaatccc
     4561 cgagaccccc cacgttggtc tgtgctagct ggccactctc ggactgtctc agattccatc
     4621 aagaaactta ttacaagcat gagggacaaa gccccagggc agctggagtg tgagacagcc
     4681 attgcggctc tgaacagctg cttgcgggac ctagatcagg cttcgcttgc cgctgtcagc
     4741 cagcagcttg ctccccgtga aggaatctct caagaggctt tgcacaccca gatgctcact
     4801 gcagtgcaag aaatttctca tctcattgag ccgctggcca gcgctgctcg ggctgaagcc
     4861 tcccagctgg gacacaaggt gtcccaaatg gcccagtact ttgaaccact caccctggct
     4921 gcagtgggtg ctgcgtctaa gaccctgagc cacccgcagc agatggcact tctggaccag
     4981 actaaaacgt tggcagagtc tgccttgcag ttgctatata ctgccaagga ggctggtggt
     5041 aaccccaagc aagcagcaca cacccaggaa gccctggagg aggctgtgca gatgatgaca
     5101 gaggccgtag aagacctgac gacgaccctc aacgaagcag ccagtgctgc aggagtcgtt
     5161 ggcggcatgg tggactctat cactcaggcc atcaaccagc tagatgaagg acctatgggt
     5221 gacccagaag gctcattcgt agattaccag acaaccatgg tgaggacagc caaggccatt
     5281 gctgtcactg ttcaggagat ggtaaccaag tcaaacacca gccctgaaga gctaggccct
     5341 cttgccaacc agctgaccag tgactatggc cgactggcct cacaagccaa gcctgcagct
     5401 gtggctgctg aaaatgaaga gataggcgct cacatcaaac accgagtaca ggagctgggc
     5461 catggctgct ctgctctggt caccaaggca ggtgccttgc agtgtagccc cagtgatgtc
     5521 tacaccaaga aggagctcat agagtgtgcc cgcagagtgt cagagaaggt ctcccatgtt
     5581 ctggctgcac tccaggctgg gaatcgtggt acccaggcct gcattacagc agccagtgct
     5641 gtgtctggta tcattgctga cctcgacacc accatcatgt tcgctactgc tggcacactt
     5701 aaccgtgagg gtgctgaaac ttttgctgac caccgggagg gtatcttaaa gacagcaaag
     5761 gttcttgtgg aggacaccaa ggtcctagtg cagaatgcag ctgggagcca ggagaagttg
     5821 gcacaagccg cccagtcctc cgtggccacc attacccgcc tcgctgatgt ggtcaagctc
     5881 ggtgcagcca gcctaggagc cgaagaccct gaaactcagg tggtgctgat caatgcagta
     5941 aaggacgtag ccaaggccct gggtgacctc atcagcgcta cgaaggctgc agcgggcaaa
     6001 gttggggatg accctgcagt gtggcagctc aagaactctg ccaaggtgat ggtgaccaat
     6061 gtgacatcat tgctcaagac agtgaaggct gtggaagatg aggccaccaa aggcacacgg
     6121 gccctagagg caaccacaga gcacatacgt caggaactgg cggtcttctg ttccccagag
     6181 ccacctgcca agacctctac ccctgaagac ttcatccgaa tgaccaaggg tattactatg
     6241 gcaacagcca aagccgttgc tgctggcaat tcctgtcgac aggaagatgt cattgccaca
     6301 gccaatctga gccgacgggc tattgcggac atgcttcggg cttgcaagga agcagctttc
     6361 cacccagaag tggcgcctga tgtacggctc cgagccctgc actatggccg ggagtgtgcc
     6421 aatggttacc tggaactgct ggaccacgtg ctgctgaccc ttcagaagcc aaacccagac
     6481 ctgaagcagc agctaactgg acactcaaag cgggttgctg gctctgtgac tgagctcatc
     6541 caggctgctg aagccatgaa aggaacagag tgggtggacc cagaggaccc tactgtcatt
     6601 gctgagaatg agctcttggg agccgcagcc gccatcgagg ctgcagccaa gaagctggag
     6661 cagctgaagc ccagggccaa acccaaggag gcggatgagt ccttgaactt tgaggaacaa
     6721 atcctagaag ctgccaagtc catcgctgca gccaccagtg cactggtaaa ggctgcgtca
     6781 gcagcccaga gggagctggt ggctcaagga aaggtgggcg ccattccagc caatgcactg
     6841 gatgatgggc agtggtcgca gggcctcatt tccgctgccc gtatggtggc tgcggccacc
     6901 aacaatctgt gtgaagcagc taacgcagct gtccagggcc atgctagcca ggagaaactc
     6961 atttcctcag ccaagcaagt agctgcctcc acagctcagc tcctggtagc ttgcaaggtc
     7021 aaagcagatc aggactctga ggcaatgaaa cggctccagg ctgctggcaa tgcagtgaag
     7081 agggcctcag ataacctggt aaaggcggcc cagaaagctg cagccttcga agaccaggag
     7141 aatgagacgg tggtggtgaa ggagaaaatg gttgggggca ttgcccagat tatcgcagca
     7201 caggaagaga tgcttcggaa ggaacgagag ctggaagagg ctcggaaaaa gctcgcccag
     7261 atccggcagc agcagtacaa gttcttgcct tcagagcttc gagacgagca cggttccggt
     7321 ggttccggcg gaagtggcat ggtgtctaaa ggcgaggccg tgatcaaaga attcatgcgg
     7381 ttcaaggtgc acatggaagg ctccatgaac ggccacgagt tcgagatcga aggcgaaggc
     7441 gagggcagac catacgaggg aacacagacc gccaagctga aagtgaccaa aggcggccct
     7501 ctgcctttca gctgggacat cctgtctcca cagtttatgt acggcagcag ggccttcatt
     7561 aagcaccccg ctgacatccc cgactactac aagcagagct tccccgaggg cttcaagtgg
     7621 gagagagtga tgaactttga ggatggcggc gctgtgaccg tgacacagga cacaagtctg
     7681 gaagatggca ccctgatcta caaagtgaag ctgaggggca caaacttccc tccagacggc
     7741 cccgtgatgc agaaaaagac catgggctgg gaagccagca ccgagagact gtatcctgag
     7801 gacggtgtcc tgaagggcga catcaagatg gccctgagac tgaaggacgg cggcagatac
     7861 ctggccgact tcaagaccac ctacaaggcc aagaaacccg tgcagatgcc tggcgcctac
     7921 aacgtggaca gaaagctgga catcaccagc cacaacgagg actacaccgt ggtggaacag
     7981 tacgagagaa gcgaggggag acacagcaca ggcggaatgg acgagctgta caagtgataa
     8041 taaccgcggg cccgggatcc accggatcta gataactgat cataatcagc cataccacat
     8101 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     8161 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     8221 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8281 tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa
     8341 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     8401 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     8461 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     8521 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     8581 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     8641 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     8701 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     8761 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     8821 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     8881 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     8941 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     9001 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9061 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     9121 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     9181 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     9241 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     9301 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     9361 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     9421 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     9481 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     9541 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     9601 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     9661 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     9721 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     9781 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     9841 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     9901 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     9961 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
    10021 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
    10081 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
    10141 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
    10201 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
    10261 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
    10321 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
    10381 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
    10441 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
    10501 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
    10561 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    10621 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10681 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10741 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10801 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    10861 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10921 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10981 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    11041 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    11101 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    11161 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    11221 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    11281 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    11341 tgttctttcc tgcgttatcc cctgattctg tgga
//
