LOCUS       sequence-417758-        3816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear-localized and catalytically-inactive E3-DART ligase in 
            domestication vector. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Huang L, Rojas-Pierce M
  TITLE     Rapid depletion of target proteins in plants by an inducible protein
            degradation system.
  JOURNAL   Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072.
  PUBMED    38446628
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2024 Mar 6:koae072. doi: 10.1093/plcell/koae072."
COMMENT     Sequence Label: pUPD2-3xNLS-HA-LRR-NELC492A-C (B3-B4)
FEATURES             Location/Qualifiers
     source          1..3816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             25..45
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             46..66
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             67..87
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             88..114
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             236..247
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     terminator      1773..1830
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1854..1871)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2025..2613)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2105..2124)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2796..2890)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2911..3570)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3485..3504
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3732..3750
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3754..3797)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(3800..3816,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg aatgcctaag aaaaagagaa aggttcctaa gaaaaagaga
       61 aaggttccta aaaagaaaag aaaggtttac ccatacgatg ttccagatta cgctgagtat
      121 gatgctgtct ggtcaaaatg ggaaagggat gcaccagcag gagagtcacc tggaagagca
      181 gcagtggttc aggaaatgcg tgattgcctt aataacggaa atccagtgct taacgtggga
      241 gcttcaggtc ttaccaccct tccagaccgt cttccaccgc atattacaac acttgttatt
      301 ccagataata atctcacctc tttgcctgag ttgccagaag gacttagaga gcttgaggtg
      361 tctggaaacc ttcagcttac atctcttcca tctcttccgc agggtcttca gaagttgtgg
      421 gcctataaca attggcttgc ctctcttccg actttgccgc caggacttgg ggatcttgct
      481 gtctctaata accagcttac ctctcttccg gagatgccgc cagcacttag agagcttagg
      541 gtgtctggta acaaccttac ctctcttccg gctcttccgt caggacttca gaagctttgg
      601 gcatataaca atagattgac ctctttgcca gagatgtcac caggtcttca ggaacttgat
      661 gtctctcata atcagcttac cagattgccg caatctctca ctggtctttc ttctgctgca
      721 agagtttatc ttgacgggaa tccactttct gttagaactc ttcaggctct tagagacatc
      781 attggacatt caggaatcag gatacacttc gatatggctg ggccttctgt ccctagagaa
      841 gccagagcac ttcaccttgc tgtcgctgac tggcttactt ctgcaagaga gggggaagct
      901 gcccaggcag acagatggca ggctttcgga cttgaagata acgccgccgc cttctctctt
      961 gtccttgaca gacttcgtga gactgaaaac ttcaaaaaag acgctggatt taaggcacag
     1021 atatcatctt ggcttacaca acttgctgaa gatgctgctc ttagagcaaa aacctttgcc
     1081 atggcaacag aggcaacatc aaccgctgag gacagagtca cacatgccct tcaccagatg
     1141 aataacgttc aacttgttca taatgcagaa aaaggggaat acgacaacaa tctccagggg
     1201 cttgtttcta ctgggcgtga gatgttcaga cttgcaacac ttgaacagat tgccagagaa
     1261 aaagccggaa cacttgctct tgtcgatgac gttgaggtct atcttgcttt ccagaataag
     1321 cttaaggaat cacttgagct tacctctgtg acttcagaaa tgcgtttctt tgacgtttct
     1381 ggagtgactg tttcagacct tcaggctgct gagcttcagg tgaaaaccgc tgaaaactct
     1441 gggttcagta aatggatact tcagtggggg ccgcttcact ctgtgcttga aagaaaagtg
     1501 ccggaaagat ttaacgctct tcgtgaaaag caaatatctg attatgaaga cacttacaga
     1561 aagctttatg acgaagtgct taaatcttct gggcttgtcg acgataccga tgcagaacgt
     1621 actatcggag ttagtgctat ggatagtgct aaaaaagaat ttcttgatgg acttagagct
     1681 cttgtggatg aggtgcttgg ttcttatctt acagccagat ggcgtcttaa cttcgtgaga
     1741 ccactcatcg cgactagtag cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca
     1801 ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc
     1861 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1921 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1981 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca caggctccgc
     2041 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2101 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2161 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2221 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2281 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2341 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2401 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2461 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2521 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2581 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2641 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2701 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     2761 tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg
     2821 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     2881 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact cgtcgcagta
     2941 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     3001 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     3061 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     3121 ccagggattg gctgatacga aaaacatatt ctcaataaac cctttaggga aataggccag
     3181 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     3241 gtggtattca ctccagagtg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     3301 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     3361 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     3421 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     3481 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     3541 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     3601 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     3661 tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt atcatgacat
     3721 taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt
     3781 tcgctaagga tgatttctgg aattcgcggc cgcttc
//
