LOCUS       sequence-417760-        2345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CDS for mutant HR1b degron in domestication vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Huang L, Rojas-Pierce M
  TITLE     Rapid depletion of target proteins in plants by an inducible protein
            degradation system.
  JOURNAL   Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072.
  PUBMED    38446628
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2024 Mar 6:koae072. doi: 10.1093/plcell/koae072."
COMMENT     Sequence Label: pUPD2-HR1bR181A R185A-C (B3-B4)
FEATURES             Location/Qualifiers
     source          1..2345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      302..359
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(383..400)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(554..1142)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(634..653)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1325..1419)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1440..2099)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2014..2033
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2261..2279
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2283..2326)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(2329..2345,1..5)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tagagcgatg agtggtctcg aatggctact aatctttcta gagttgctgg acttgaaaag
       61 caacttgcta ttgaacttaa ggttaagcaa ggtgctgaaa atatgattca aacttattct
      121 aatggatcta ctaaggatag aaagcttctt cttactgctc aacaaatgct tcaagattct
      181 aagactaaga ttgatattat tgcaatgcaa cttgcaagag ctcttcaagc tggtcaactt
      241 gaaaatcaag ctgctcctgg ttcgtgagac cactcatcgc gactagtagc ggccgctgca
      301 gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat
      361 tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
      421 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
      481 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
      541 cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct
      601 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
      661 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
      721 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
      781 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
      841 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
      901 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
      961 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1021 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     1081 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     1141 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     1201 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     1261 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc
     1321 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     1381 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     1441 tacgccccgc cctgccactc gtcgcagtac tgttgtaatt cattaagcat tctgccgaca
     1501 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     1561 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     1621 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgatacgaa aaacatattc
     1681 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     1741 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt
     1801 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     1861 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     1921 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     1981 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     2041 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     2101 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     2161 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga
     2221 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
     2281 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc
     2341 gcttc
//
