LOCUS       sequence-417761-        9781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus backbone expressing mCherry and GFP1-10 fusion protein 
            with a mitochondrial localization signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9781)
  AUTHORS   Fu Y, Sacco O, DeBitetto E, Kanshin E, Ueberheide B, Sfeir A
  TITLE     Mitochondrial DNA breaks activate an integrated stress response to 
            reestablish homeostasis.
  JOURNAL   Mol Cell. 2023 Oct 8:S1097-2765(23)00753-0. doi: 
            10.1016/j.molcel.2023.09.026.
  PUBMED    37832546
REFERENCE   2  (bases 1 to 9781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2023 Oct 8:S1097-2765(23)00753-0. doi: 
            10.1016/j.molcel.2023.09.026."
COMMENT     Sequence Label: pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10
FEATURES             Location/Qualifiers
     source          1..9781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     59..78
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    699..1287
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(752..772)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1170..1181)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1435..1615
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1662..1680)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1748..1852
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1853..1921,1922..2713)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2071..2090)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2884..3472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3626..3643
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3883..3904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3919..3924,3925..3942,3943..3949)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3957..3973
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3962..3984
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3981..3997
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3981..3997
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4047..4066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4258..4891
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4938..5063
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5560..5793
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5978..6022
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6315..6432
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6480..7658
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6711..7649
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7606..7626
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7980..7989
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(9557..9781,1..237)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9613..9630)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
       61 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
      121 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
      181 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatac
      241 catgaaaaca tttaacattt ctcaacagga tctagaatta gtagaagtag cgacagagaa
      301 gattacaatg ctttatgagg ataataaaca tcatgtggga gcggcaattc gtacgaaaac
      361 aggagaaatc atttcggcag tacatattga agcgtatata ggacgagtaa ctgtttgtgc
      421 agaagccatt gcgattggta gtgcagtttc gaatggacaa aaggattttg acacgattgt
      481 agctgttaga cacccttatt ctgacgaagt agatagaagt attcgagtgg taagtccttg
      541 tggtatgtgt agggagttga tttcagacta tgcaccagat tgttttgtgt taatagaaat
      601 gaatggcaag ttagtcaaaa ctacgattga agaactcatt ccactcaaat atacccgaaa
      661 ttaaaagttt taccatacca agctcatcga tagatcctaa tcaacctctg gattacaaaa
      721 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
      781 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
      841 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
      901 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
      961 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     1021 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     1081 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     1141 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     1201 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     1261 ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac ttacaaggca
     1321 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     1381 caacgaagac aagatcacct gcaggacagg cgcgccctgc tttttgcttg tactgggtct
     1441 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     1501 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     1561 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcacccgg
     1621 gcgattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt
     1681 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     1741 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1801 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1861 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1921 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     1981 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2041 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     2101 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2161 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2221 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2281 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2341 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2401 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2461 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2521 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2581 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2641 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2701 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     2761 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     2821 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     2881 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2941 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3001 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3061 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3121 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3181 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3241 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3301 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3361 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3421 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3481 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3541 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3601 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     3661 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc
     3721 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3781 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga
     3841 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     3901 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     3961 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa
     4021 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4081 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
     4141 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
     4201 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagttgg
     4261 aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac
     4321 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga
     4381 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata
     4441 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     4501 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc
     4561 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg
     4621 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct
     4681 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     4741 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4801 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4861 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     4921 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     4981 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     5041 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     5101 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     5161 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     5221 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     5281 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     5341 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     5401 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     5461 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     5521 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     5581 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     5641 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     5701 acagcatctg ttgcaactca cagtctgggg catcaagcag cttcaggcaa gaatcctggc
     5761 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     5821 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     5881 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     5941 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     6001 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     6061 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     6121 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     6181 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     6241 agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaattc acaaatggca
     6301 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     6361 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     6421 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggac tagtcgtgag
     6481 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     6541 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     6601 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     6661 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     6721 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     6781 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     6841 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     6901 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     6961 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     7021 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     7081 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     7141 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     7201 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     7261 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     7321 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     7381 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     7441 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     7501 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     7561 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     7621 tggttcaaag tttttttctt ccatttcagg tgtcgtgaag cggccgctta attaagttta
     7681 aacggatccc gccggcggct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
     7741 ctgcagtcga catggcctcc actcgtgtcc tcgcctctcg cctggcctcc cggatggctg
     7801 cttccgccaa ggttgcccgc cctgctgtcc gcgttgctca ggtcagcaag cgcaccatcc
     7861 agactggctc ccccctccag accctcaagc gcacccagat gacctccatc gtcaacgcca
     7921 ccacccgcca ggctttccag aagcgcgcct actcttccgg atccggtgac ggtccggtcg
     7981 ccaccatggt ttctaaaggt gaagaagata atatggctat tattaaagaa tttatgagat
     8041 ttaaagttca tatggaaggt tctgttaatg gtcatgaatt tgaaattgaa ggtgaaggtg
     8101 aaggtagacc atatgaaggt actcaaactg ctaaattgaa agttactaaa ggtggtccat
     8161 tgccatttgc ttgggatatt ttgtctccac aatttatgta tggttctaaa gcttatgtta
     8221 aacatccagc tgatattcca gattatttga aattgtcttt tccagaaggt tttaaatggg
     8281 aaagagttat gaattttgaa gatggtggtg ttgttactgt tactcaagat tcttctttgc
     8341 aagatggtga atttatttat aaagttaaat tgagaggtac taattttcca tctgatggtc
     8401 cagttatgca aaaaaaaact atgggttggg aagcttcttc tgaaagaatg tatccagaag
     8461 atggtgcttt gaaaggtgaa attaaacaaa gattgaaatt gaaagatggt ggtcattatg
     8521 atgctgaagt taaaactact tataaagcta aaaaaccagt tcaattgcca ggtgcttata
     8581 atgttaatat taaattggat attacttctc ataatgaaga ttatactatt gttgaacaat
     8641 atgaaagagc tgaaggtaga cattctactg gtggtatgga tgaattgtat aaagaattcg
     8701 gtggtggttc tggcggaggt tcaatgtcta aaggtgaaga attgtttact ggtgttgttc
     8761 caattttggt tgaattggat ggtgatgtta atggtcataa attttctgtt agaggtgaag
     8821 gtgaaggtga tgctactatt ggtaaattga ctttgaaatt tatttgtact actggtaaat
     8881 tgccagttcc atggccaact ttggttacta ctttgactta tggtgttcaa tgtttttcta
     8941 gatatccaga tcatatgaaa caacatgatt tttttaaatc tgctatgcca gaaggttatg
     9001 ttcaagaaag aactatttct tttaaagatg atggtaaata taaaactaga gctgttgtta
     9061 aatttgaagg tgatactttg gttaatagaa ttgaattgaa aggtactgat tttaaagaag
     9121 atggtaatat tttgggtcat aaattggaat ataattttaa ttctcataat gtttatatta
     9181 ctgctgataa acaaaaaaat ggtattaaag ctaattttac tgttagacat aatgttgaag
     9241 atggttctgt tcaattggct gatcattatc aacaaaatac tccaattggt gatggtccag
     9301 ttttgttgcc agataatcat tatttgtcta ctcaaactgt tttgtctaaa gatccaaatg
     9361 aaaaataaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
     9421 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     9481 tgttgttgtt gtatctagag ctcgaggtcg accaattccg gttattttcc accatattgc
     9541 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     9601 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     9661 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     9721 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     9781 c
//
