LOCUS       sequence-417766-        9183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus backbone expressing SEC61B protein tagged with 4 tandem 
            GFP11 repeats at the N-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9183)
  AUTHORS   Fu Y, Sacco O, DeBitetto E, Kanshin E, Ueberheide B, Sfeir A
  TITLE     Mitochondrial DNA breaks activate an integrated stress response to 
            reestablish homeostasis.
  JOURNAL   Mol Cell. 2023 Oct 8:S1097-2765(23)00753-0. doi: 
            10.1016/j.molcel.2023.09.026.
  PUBMED    37832546
REFERENCE   2  (bases 1 to 9183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2023 Oct 8:S1097-2765(23)00753-0. doi: 
            10.1016/j.molcel.2023.09.026."
COMMENT     Sequence Label: pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro
FEATURES             Location/Qualifiers
     source          1..9183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    400..988
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(453..473)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(871..882)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1136..1316
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1363..1381)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1449..1553
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1554..1622,1623..2414)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1772..1791)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2585..3173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3327..3344
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3584..3605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3620..3625,3626..3643,3644..3650)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3658..3674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3663..3685
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3682..3698
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3682..3698
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3748..3767
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             3959..4592
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4639..4764
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5261..5494
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5679..5723
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6016..6133
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6181..7359
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6412..7350
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7307..7327
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7420..7467
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             7489..7536
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             7552..7599
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             7615..7662
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             7669..7956
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="PGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAGR
                     TTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     misc_feature    8057..8518
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8113..8130)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8340..8359
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8523..9183,1..365)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             join(8523..9183,1..365)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
       61 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
      121 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
      181 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
      241 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
      301 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
      361 aatagagtag atgccgaccg ggatctatcg atagatccta atcaacctct ggattacaaa
      421 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
      481 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
      541 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
      601 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
      661 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
      721 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
      781 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
      841 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
      901 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
      961 cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc
     1021 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     1081 ccaacgaaga caagatcacc tgcaggacag gcgcgccctg ctttttgctt gtactgggtc
     1141 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     1201 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     1261 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcacccg
     1321 ggcgattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat
     1381 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1441 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1501 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1561 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1621 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1681 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1741 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     1801 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1861 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1921 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1981 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2041 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2101 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2161 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2221 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2281 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2341 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2401 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2461 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2521 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2581 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2641 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2701 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2761 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2821 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2881 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2941 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3001 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3061 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3121 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3181 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3241 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3301 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3361 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg
     3421 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     3481 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg
     3541 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     3601 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     3661 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata
     3721 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3781 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
     3841 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
     3901 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
     3961 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
     4021 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     4081 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     4141 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     4201 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     4261 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     4321 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     4381 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     4441 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     4501 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     4561 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     4621 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     4681 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     4741 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     4801 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     4861 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     4921 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     4981 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     5041 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     5101 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     5161 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     5221 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     5281 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     5341 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     5401 aacagcatct gttgcaactc acagtctggg gcatcaagca gcttcaggca agaatcctgg
     5461 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     5521 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     5581 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     5641 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     5701 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     5761 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     5821 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     5881 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     5941 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccgaatt cacaaatggc
     6001 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     6061 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     6121 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga ctagtcgtga
     6181 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     6241 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     6301 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     6361 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     6421 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     6481 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     6541 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     6601 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     6661 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     6721 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     6781 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     6841 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     6901 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     6961 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     7021 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     7081 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     7141 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     7201 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     7261 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     7321 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccgctt aattaagttt
     7381 aaacggatcc cgccggcggc tagcgttaac acgcgtatgc gtgaccacat ggtccttcat
     7441 gagtatgtaa atgctgctgg gattacagct agaggtgggt cgggcggacg agaccatatg
     7501 gtgcttcacg aatacgtaaa cgcagctggc attactggcg gatcaggtgg cagggatcac
     7561 atggtactcc atgagtacgt gaacgctgct ggaatcacag gcggtagcgg cggtcgggac
     7621 catatggtcc tgcacgaata tgtcaatgct gccggtatca ccgctagccc tggtccgacc
     7681 cccagtggca ctaacgtggg atcctcaggg cgctctccca gcaaagcagt ggccgcccgg
     7741 gcggcgggat ccactgtccg gcagaggaaa aatgccagct gtgggacaag gagtgcaggc
     7801 cgcacaacct cggcaggcac cggggggatg tggcgattct acacagaaga ttcacctggg
     7861 ctcaaagttg gccctgttcc agtattggtt atgagtcttc tgttcatcgc ttctgtattt
     7921 atgttgcaca tttggggcaa gtacactcgt tcgtagaagc ttggtaccga gctcggatcc
     7981 actagtccag tgtggtggaa ttctgcgtcg accaattccg gttattttcc accatattgc
     8041 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     8101 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     8161 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     8221 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     8281 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     8341 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     8401 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     8461 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     8521 ccatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg
     8581 acagcgtctc cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg
     8641 atgtaggagg gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag
     8701 atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca
     8761 ttggggaatt tagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt
     8821 tgcaagacct gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg
     8881 atgcgatcgc tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag
     8941 gaatcggtca atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt
     9001 atcactggca aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg
     9061 agctgatgct ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg
     9121 gctccaacaa tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg
     9181 cga
//
