LOCUS       sequence-417770-        8851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus backbone expressing GFP1-10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Fu Y, Sacco O, DeBitetto E, Kanshin E, Ueberheide B, Sfeir A
  TITLE     Mitochondrial DNA breaks activate an integrated stress response to 
            reestablish homeostasis.
  JOURNAL   Mol Cell. 2023 Oct 8:S1097-2765(23)00753-0. doi: 
            10.1016/j.molcel.2023.09.026.
  PUBMED    37832546
REFERENCE   2  (bases 1 to 8851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2023 Oct 8:S1097-2765(23)00753-0. doi: 
            10.1016/j.molcel.2023.09.026."
COMMENT     Sequence Label: pHAGE2-EF1a-GFP1-10-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..8851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    87..204
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        252..1430
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          483..1421
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1378..1398
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1449..2087
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGK
                     YKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
     misc_feature    2222..2683
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2278..2295)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2505..2524
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2688..3287
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2688..2707)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3184..3204
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3322..3910
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3375..3395)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3793..3804)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4058..4238
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4285..4303)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4371..4475
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4476..4544,4545..5336)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4694..4713)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5507..6095
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6249..6266
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6506..6527
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6542..6547,6548..6565,6566..6572)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6580..6596
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6585..6607
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6604..6620
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6604..6620
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6670..6689
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6881..7514
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7561..7686
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8183..8416
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8601..8645
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat
       61 tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
      121 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
      181 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
      241 actagtcgtg aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
      301 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
      361 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
      421 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
      481 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
      541 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
      601 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
      661 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
      721 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
      781 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
      841 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
      901 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
      961 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
     1021 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
     1081 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     1141 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     1201 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     1261 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     1321 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     1381 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga agcggccgca
     1441 tgggatcctc caaaggagaa gaactgttta ccggtgttgt gccaattttg gttgaactcg
     1501 atggtgatgt caacggacat aagttctcag tgagaggcga aggagaaggt gacgccacca
     1561 ttggaaaatt gactcttaaa ttcatctgta ctactggtaa acttcctgta ccatggccga
     1621 ctctcgtaac aacgcttacg tacggagttc agtgcttttc gagataccca gaccatatga
     1681 aaagacatga cttttttaag tcggctatgc ctgaaggtta cgtgcaagaa agaacaattt
     1741 cgttcaaaga tgatggaaaa tataaaacta gagcagttgt taaatttgaa ggagatactt
     1801 tggttaaccg cattgaactg aaaggaacag attttaaaga agatggtaat attcttggac
     1861 acaaactcga atacaatttt aatagtcata acgtatacat cactgctgat aagcaaaaga
     1921 acggaattaa agcgaatttc acagtacgcc ataatgtaga agatggcagt gttcaacttg
     1981 ccgaccatta ccaacaaaac acccctattg gagacggtcc ggtacttctt cctgataatc
     2041 actacctctc aacacaaaca gtcctgagca aagatccaaa tgaaaaagga acaggtggcg
     2101 gcggaagtta gctagcgctc tgcagaaaaa actcgaggag ctggaactgg attcttaatc
     2161 tagagctcga ggtcgaccaa ttccggttat tttccaccat attgccgtct tttggcaatg
     2221 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     2281 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     2341 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     2401 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     2461 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     2521 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     2581 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc
     2641 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taataccatg accgagtaca
     2701 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     2761 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     2821 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     2881 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     2941 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     3001 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     3061 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     3121 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     3181 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     3241 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaagt agatgccgac
     3301 cgggatctat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact
     3361 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     3421 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     3481 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     3541 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     3601 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     3661 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     3721 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     3781 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     3841 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     3901 gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     3961 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatca
     4021 cctgcaggac aggcgcgccc tgctttttgc ttgtactggg tctctctggt tagaccagat
     4081 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     4141 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     4201 cctcagaccc ttttagtcag tgtggaaaat ctctagcacc cgggcgatta aggaaagggc
     4261 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     4321 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4381 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4441 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4501 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4561 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4621 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4681 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     4741 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4801 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4861 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4921 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4981 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5041 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5101 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5161 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5221 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5281 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5341 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5401 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5461 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5521 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5581 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5641 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5701 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5761 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5821 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5881 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5941 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6001 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6061 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6121 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6181 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6241 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6301 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat
     6361 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6421 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga
     6481 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6541 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6601 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc
     6661 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
     6721 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
     6781 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
     6841 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc
     6901 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     6961 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
     7021 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
     7081 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
     7141 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
     7201 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
     7261 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
     7321 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7381 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7441 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7501 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     7561 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     7621 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     7681 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     7741 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     7801 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     7861 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     7921 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7981 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct
     8041 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     8101 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     8161 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     8221 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     8281 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     8341 tcacagtctg gggcatcaag cagcttcagg caagaatcct ggctgtggaa agatacctaa
     8401 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     8461 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     8521 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     8581 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     8641 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     8701 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     8761 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     8821 acaggcccga aggaatagaa gaagaaggtg g
//
