LOCUS       sequence-417777-        9795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prime editing in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Jang G, Kweon J, Kim Y
  TITLE     CRISPR prime editing for unconstrained correction of oncogenic KRAS 
            variants.
  JOURNAL   Commun Biol. 2023 Jun 30;6(1):681. doi: 10.1038/s42003-023-05052-1.
  PUBMED    37391511
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-06-30"; volume: "6(1):681. doi"; pages: " 
            10.1038/s42003-023-05052-1"
COMMENT     Sequence Label: pCMV-PEmax-SpG
FEATURES             Location/Qualifiers
     source          1..9795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        226..429
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     380..400
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     471..490
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        471..489
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             539..559
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             560..4660
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4718..4738
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6839..6859
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6869..6895
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             6904..6921
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6944..6961)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6950..7174
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7245..7261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7245..7261)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7258..7280)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7269..7285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7293..7299,7300..7317,7318..7323))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7338..7359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7476..7493)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7647..8235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7727..7746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8406..9197,9198..9266))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9029..9048
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9267..9371)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9450..9469)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(9641..9795,1..225)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
       61 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      121 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      181 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      241 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      301 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      361 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      421 agcagagctg gtttagtgaa ccgtcagatc cgctagagat ccgcggccgc taatacgact
      481 cactataggg agagccgcca ccatgaaacg gacagccgac ggaagcgagt tcgagtcacc
      541 aaagaagaag cggaaagtcg acaagaagta cagcatcggc ctggacatcg gcaccaactc
      601 tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct
      661 gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag
      721 cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg
      781 gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga
      841 cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg
      901 gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat
      961 ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta
     1021 tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa
     1081 ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct
     1141 gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag
     1201 actgagcaag agcagaaagc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa
     1261 tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa
     1321 cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct
     1381 ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa
     1441 cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc
     1501 ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct
     1561 gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag
     1621 caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt
     1681 catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaagag
     1741 agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca
     1801 cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga
     1861 caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct
     1921 ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc
     1981 ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat
     2041 gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta
     2101 cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag
     2161 aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac
     2221 caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt
     2281 cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca
     2341 cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat
     2401 tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg
     2461 gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag
     2521 atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc
     2581 cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca
     2641 gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg
     2701 ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa
     2761 gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa
     2821 gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa
     2881 gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat
     2941 cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta
     3001 cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga
     3061 ctacgatgtg gacgctatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa
     3121 ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt
     3181 cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag
     3241 aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg
     3301 cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct
     3361 ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt
     3421 gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt
     3481 gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac
     3541 cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt
     3601 gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa
     3661 gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg
     3721 cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga
     3781 taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt
     3841 gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa
     3901 cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcct
     3961 gtggcccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa
     4021 gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga
     4081 gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat
     4141 catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc
     4201 ctctgccaag cagctgcaga agggaaacga actggccctg ccctccaaat atgtgaactt
     4261 cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa
     4321 acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga
     4381 gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa
     4441 caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct
     4501 gaccaatctg ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagca
     4561 gtacagaagc accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct
     4621 gtacgagaca cggatcgacc tgtctcagct gggaggtgac tccggcggaa gctctggtgg
     4681 cagcaagcgg accgccgacg gctctgaatt cgagagccct aagaagaaaa gaaaggtgag
     4741 cggaggctct agcggcggaa gcaccctgaa cattgaagac gagtatagac tgcatgaaac
     4801 aagcaaggaa cccgacgtgt ccctgggctc cacctggctg tccgactttc cccaggcctg
     4861 ggccgagaca ggaggaatgg gcctggccgt gcggcaggca cccctgatca tccctctgaa
     4921 ggccacctct acacccgtga gcatcaagca gtaccctatg tctcaggagg ccagactggg
     4981 catcaagcct cacatccaga ggctgctgga ccagggcatc ctggtgccat gccagagccc
     5041 ctggaacaca ccactgctgc ccgtgaagaa gccaggcacc aatgactata gacccgtgca
     5101 ggatctgaga gaggtgaaca agagggtgga ggatatccac cccaccgtgc ccaaccctta
     5161 caatctgctg tccggcctgc ccccttctca ccagtggtat acagtgctgg acctgaagga
     5221 tgccttcttt tgtctgagac tgcaccctac cagccagcca ctgttcgcct ttgagtggag
     5281 ggaccctgag atgggcatct ctggccagct gacctggaca cgcctgcctc agggcttcaa
     5341 gaatagccca acactgttta acgaggccct gcaccgcgac ctggcagatt tccggatcca
     5401 gcacccagat ctgatcctgc tgcagtacgt ggacgatctg ctgctggccg ccaccagcga
     5461 gctggattgc cagcagggaa cacgcgccct gctgcagacc ctgggaaacc tgggatatag
     5521 ggcatccgcc aagaaggccc agatctgtca gaagcaggtg aagtacctgg gctatctgct
     5581 gaaggagggc cagagatggc tgacagaggc caggaaggag acagtgatgg gccagccaac
     5641 acccaagacc ccaagacagc tgagggagtt cctgggcaaa gcaggatttt gcaggctgtt
     5701 catcccagga ttcgcagaga tggcagcacc tctgtaccca ctgaccaagc cgggcaccct
     5761 gtttaattgg ggccctgacc agcagaaggc ctatcaggag atcaagcagg ccctgctgac
     5821 agcaccagcc ctgggcctgc cagacctgac caagcctttc gagctgtttg tggatgagaa
     5881 gcagggctac gccaagggcg tgctgaccca gaagctggga ccatggagac ggcccgtggc
     5941 ctatctgtcc aagaagctgg acccagtggc agcaggatgg ccaccatgcc tgaggatggt
     6001 ggcagcaatc gccgtgctga caaaggatgc cggcaagctg accatgggac agccactggt
     6061 catcctggca ccacacgcag tggaggccct ggtgaagcag cctccagatc gctggctgtc
     6121 taacgcccgg atgacacact accaggccct gctgctggac accgatcgcg tgcagtttgg
     6181 ccctgtggtg gccctgaatc cagccaccct gctgcctctg ccagaggagg gcctgcagca
     6241 caactgtctg gacatcctgg cagaggcaca cggaacaagg ccagacctga ccgatcagcc
     6301 cctgcctgac gccgatcaca catggtatac cgatggaagc tccctgctgc aggagggcca
     6361 gaggaaggca ggagcagcag tgaccacaga gacagaagtg atctgggcca aggccctgcc
     6421 agcaggcaca tccgcccagc gggccgagct gatcgccctg acccaggccc tgaagatggc
     6481 cgagggcaag aagctgaacg tgtacacaga ctccagatat gccttcgcca ccgcacacat
     6541 ccacggagag atctacaggc gccggggctg gctgacctct gagggcaagg agatcaagaa
     6601 caaggatgag atcctggccc tgctgaaggc cctgtttctg cccaagcggc tgagcatcat
     6661 ccactgtcct ggacaccaga agggacactc cgccgaggca aggggcaatc ggatggccga
     6721 ccaggccgcc agaaaggctg ctattactga aactcccgac acttccactc tgctgattga
     6781 aaactcctcc ccttctggcg gctcaaaaag aaccgccgac ggcagcgaat tcgagtctcc
     6841 caagaagaag aggaaagtcg gctctggccc tgccgctaag agagtgaagc tggactaacc
     6901 ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct
     6961 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     7021 actcccactg tcctttccta ataaaatgag aaaattgcat cgcattgtct gagtaggtgt
     7081 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     7141 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     7201 ggctcgatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7261 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7321 aaagcctagg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7381 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7441 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7501 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7561 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7621 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7681 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7741 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7801 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7861 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7921 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7981 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8041 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8101 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8161 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8221 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacac tcagtggaac
     8281 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8341 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8401 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8461 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8521 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8581 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8641 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8701 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8761 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8821 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8881 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8941 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9001 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9061 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9121 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9181 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9241 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9301 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9361 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcga
     9421 tctcccgatc ccctagggtc gactctcagt acaatctgct ctgatgccgc atagttaagc
     9481 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     9541 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     9601 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     9661 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     9721 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     9781 tcaataatga cgtat
//
