LOCUS       sequence-417778-       12117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prime editing in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12117)
  AUTHORS   Jang G, Kweon J, Kim Y
  TITLE     CRISPR prime editing for unconstrained correction of oncogenic KRAS 
            variants.
  JOURNAL   Commun Biol. 2023 Jun 30;6(1):681. doi: 10.1038/s42003-023-05052-1.
  PUBMED    37391511
REFERENCE   2  (bases 1 to 12117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-06-30"; volume: "6(1):681. doi"; pages: " 
            10.1038/s42003-023-05052-1"
COMMENT     Sequence Label: pCMV-PEmax-SpG-P2A-hMLH1dn
FEATURES             Location/Qualifiers
     source          1..12117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(520..1311,1312..1380))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1143..1162
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1381..1485)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1564..1583)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1755..2134
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2135..2338
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2289..2309
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2380..2399
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2380..2398
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2448..2468
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2469..6569
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6627..6647
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8748..8768
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8778..8804
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             8814..8870
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             11135..11152
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(11175..11192)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11181..11405
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11476..11492)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11476..11492)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11489..11511)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11500..11516
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11524..11530,11531..11548,11549..11554))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11569..11590
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11707..11724)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(11878..12117,1..349))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11958..11977)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
       61 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      121 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      181 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      241 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      301 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      361 gatcttttct acggggtctg acactcagtg gaacgaaaac tcacgttaag ggattttggt
      421 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      481 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      541 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      601 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      661 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      721 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
      781 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
      841 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
      901 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
      961 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1021 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1081 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1141 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1201 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1261 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1321 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1381 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1441 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1501 agtgccacct gacgtcgacg gatcgggaga tcgatctccc gatcccctag ggtcgactct
     1561 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     1621 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     1681 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     1741 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     1801 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     1861 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1921 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     1981 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2041 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2101 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     2161 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2221 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2281 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     2341 tttagtgaac cgtcagatcc gctagagatc cgcggccgct aatacgactc actataggga
     2401 gagccgccac catgaaacgg acagccgacg gaagcgagtt cgagtcacca aagaagaagc
     2461 ggaaagtcga caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg
     2521 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg
     2581 accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag
     2641 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga
     2701 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc
     2761 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct
     2821 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga
     2881 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg
     2941 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca
     3001 gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa
     3061 accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga
     3121 gcagaaagct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg
     3181 gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg
     3241 ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc
     3301 tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg
     3361 ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg
     3421 cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg
     3481 tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct
     3541 acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca
     3601 tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaagaga gaggacctgc
     3661 tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc
     3721 tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa
     3781 agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa
     3841 acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg
     3901 aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg
     3961 ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca
     4021 ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct
     4081 tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag
     4141 tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg
     4201 aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga
     4261 aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata
     4321 tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct
     4381 atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct
     4441 ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa
     4501 tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg
     4561 acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata
     4621 gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc
     4681 agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca
     4741 tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg
     4801 agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac
     4861 accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg
     4921 ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg
     4981 acgctatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca
     5041 gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga
     5101 tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca
     5161 atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga
     5221 gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga
     5281 tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga
     5341 agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca
     5401 acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca
     5461 aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc
     5521 ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct
     5581 acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga
     5641 agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg
     5701 attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg
     5761 aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc
     5821 tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcctg tggcccaccg
     5881 tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga
     5941 gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca
     6001 tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc
     6061 ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccaagc
     6121 agctgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg
     6181 ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg
     6241 tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga
     6301 gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg
     6361 ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg
     6421 gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagcag tacagaagca
     6481 ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac
     6541 ggatcgacct gtctcagctg ggaggtgact ccggcggaag ctctggtggc agcaagcgga
     6601 ccgccgacgg ctctgaattc gagagcccta agaagaaaag aaaggtgagc ggaggctcta
     6661 gcggcggaag caccctgaac attgaagacg agtatagact gcatgaaaca agcaaggaac
     6721 ccgacgtgtc cctgggctcc acctggctgt ccgactttcc ccaggcctgg gccgagacag
     6781 gaggaatggg cctggccgtg cggcaggcac ccctgatcat ccctctgaag gccacctcta
     6841 cacccgtgag catcaagcag taccctatgt ctcaggaggc cagactgggc atcaagcctc
     6901 acatccagag gctgctggac cagggcatcc tggtgccatg ccagagcccc tggaacacac
     6961 cactgctgcc cgtgaagaag ccaggcacca atgactatag acccgtgcag gatctgagag
     7021 aggtgaacaa gagggtggag gatatccacc ccaccgtgcc caacccttac aatctgctgt
     7081 ccggcctgcc cccttctcac cagtggtata cagtgctgga cctgaaggat gccttctttt
     7141 gtctgagact gcaccctacc agccagccac tgttcgcctt tgagtggagg gaccctgaga
     7201 tgggcatctc tggccagctg acctggacac gcctgcctca gggcttcaag aatagcccaa
     7261 cactgtttaa cgaggccctg caccgcgacc tggcagattt ccggatccag cacccagatc
     7321 tgatcctgct gcagtacgtg gacgatctgc tgctggccgc caccagcgag ctggattgcc
     7381 agcagggaac acgcgccctg ctgcagaccc tgggaaacct gggatatagg gcatccgcca
     7441 agaaggccca gatctgtcag aagcaggtga agtacctggg ctatctgctg aaggagggcc
     7501 agagatggct gacagaggcc aggaaggaga cagtgatggg ccagccaaca cccaagaccc
     7561 caagacagct gagggagttc ctgggcaaag caggattttg caggctgttc atcccaggat
     7621 tcgcagagat ggcagcacct ctgtacccac tgaccaagcc gggcaccctg tttaattggg
     7681 gccctgacca gcagaaggcc tatcaggaga tcaagcaggc cctgctgaca gcaccagccc
     7741 tgggcctgcc agacctgacc aagcctttcg agctgtttgt ggatgagaag cagggctacg
     7801 ccaagggcgt gctgacccag aagctgggac catggagacg gcccgtggcc tatctgtcca
     7861 agaagctgga cccagtggca gcaggatggc caccatgcct gaggatggtg gcagcaatcg
     7921 ccgtgctgac aaaggatgcc ggcaagctga ccatgggaca gccactggtc atcctggcac
     7981 cacacgcagt ggaggccctg gtgaagcagc ctccagatcg ctggctgtct aacgcccgga
     8041 tgacacacta ccaggccctg ctgctggaca ccgatcgcgt gcagtttggc cctgtggtgg
     8101 ccctgaatcc agccaccctg ctgcctctgc cagaggaggg cctgcagcac aactgtctgg
     8161 acatcctggc agaggcacac ggaacaaggc cagacctgac cgatcagccc ctgcctgacg
     8221 ccgatcacac atggtatacc gatggaagct ccctgctgca ggagggccag aggaaggcag
     8281 gagcagcagt gaccacagag acagaagtga tctgggccaa ggccctgcca gcaggcacat
     8341 ccgcccagcg ggccgagctg atcgccctga cccaggccct gaagatggcc gagggcaaga
     8401 agctgaacgt gtacacagac tccagatatg ccttcgccac cgcacacatc cacggagaga
     8461 tctacaggcg ccggggctgg ctgacctctg agggcaagga gatcaagaac aaggatgaga
     8521 tcctggccct gctgaaggcc ctgtttctgc ccaagcggct gagcatcatc cactgtcctg
     8581 gacaccagaa gggacactcc gccgaggcaa ggggcaatcg gatggccgac caggccgcca
     8641 gaaaggctgc tattactgaa actcccgaca cttccactct gctgattgaa aactcctccc
     8701 cttctggcgg ctcaaaaaga accgccgacg gcagcgaatt cgagtctccc aagaagaaga
     8761 ggaaagtcgg ctctggccct gccgctaaga gagtgaagct ggacggaagc ggagctacta
     8821 acttcagcct gctgaagcag gctggagacg tggaggagaa ccctggacct agcttcgttg
     8881 ctggagtcat ccggagactg gacgagacag tggtgaacag aattgccgcc ggcgaggtga
     8941 tccagagacc tgccaatgca ataaaggaga tgatcgagaa ctgtctggac gccaagtcca
     9001 caagcattca ggtgatcgtg aaggagggcg gactgaagct gatccagatc caagacaacg
     9061 gcacaggcat cagaaaggaa gatctggaca tcgtgtgtga acggttcacc acatctaagc
     9121 tgcagtcttt tgaggatctg gcctctatca gtacctacgg cttcagaggc gaggccctgg
     9181 ccagcatcag ccacgtggcc catgtgacca tcaccaccaa aaccgccgac ggcaaatgcg
     9241 cttatcgcgc tagctacagc gacggcaagc tgaaagcccc gccaaagcct tgcgccggca
     9301 accagggtac acagataaca gtggaggatc tgttctacaa catcgccacc cggagaaagg
     9361 ccctgaaaaa tcccagcgag gagtacggca agatcctgga agtcgtcggc agatactccg
     9421 tgcacaacgc cggaatcagc tttagcgtaa agaagcaggg agaaaccgtg gccgatgtgc
     9481 gcaccctgcc aaatgccagc accgtggata acatcagaag cattttcgga aatgccgtgt
     9541 ccagagaact gatcgagatc ggctgcgaag ataagaccct ggcttttaag atgaacggct
     9601 acatcagcaa cgccaattac tctgtgaaga agtgcatctt tcttctgttc atcaaccaca
     9661 gactggtgga aagcaccagc ctgcggaaag ccatcgagac agtgtacgcc gcctacctgc
     9721 ctaagaacac ccaccccttc ctgtacctga gcctcgagat cagccctcag aacgtggacg
     9781 tcaatgtgca tcctacaaag cacgaggtgc acttcctgca cgaggaaagc atcctggaaa
     9841 gagtgcagca gcacattgag agcaagctgc tgggctctaa cagcagcaga atgtacttca
     9901 cacagaccct gctgcctggc ctggccggcc cctcaggcga aatggttaag tccacaacct
     9961 ctctgacctc atccagcacc agcggttctt ccgataaggt gtacgcccac cagatggtgc
    10021 ggaccgactc tcgggagcag aagctggacg cctttctgca acctctgagc aaacctctga
    10081 gctctcagcc tcaggccatc gtgaccgagg acaagacaga tatctcctcc ggccgtgcca
    10141 gacagcagga cgaagaaatg ctcgagctgc cagctcctgc cgaggtggcc gccaagaacc
    10201 agagcctgga gggagatacc acaaagggca ccagcgaaat gagcgagaag cggggcccta
    10261 cctccagcaa ccccagaaaa cggcaccggg aggacagcga cgtggaaatg gtggaggacg
    10321 acagcagaaa ggaaatgaca gccgcttgta cccctagaag aagaatcatc aacctgacct
    10381 ccgtgctgag cctgcaggag gagatcaacg agcagggcca cgaggtgctg agagagatgc
    10441 tgcacaatca cagcttcgtg ggctgcgtga accctcaatg ggccctggct cagcatcaaa
    10501 caaagctgta cctgctgaac accaccaagc tgagcgaaga gctgttctac cagatcctca
    10561 tctacgactt cgccaacttc ggcgtgctac gcctgagcga gcccgcccct ctgtttgacc
    10621 tggccatgct ggctctggat agcccagaaa gcggctggac agaagaggac ggacctaaag
    10681 aggggctggc tgaatacatc gtggagttcc tgaagaaaaa ggccgagatg ctggccgact
    10741 acttttctct ggaaatcgac gaggaaggca acctgatcgg cctgcctctg ctgatcgata
    10801 actacgtgcc tcccctggaa ggcctgccca tcttcatcct gagactggct acagaggtga
    10861 actgggacga ggaaaaggaa tgcttcgagt ctctgagcaa ggagtgcgcc atgttctata
    10921 gcatcagaaa acagtacatc tctgaagaga gcactctgtc tggccagcag agtgaagtgc
    10981 ccggaagcat ccccaacagc tggaagtgga ccgtggaaca catcgtgtac aaggccctgc
    11041 ggagccacat tctccctcct aagcacttca ccgaggacgg caacatcctg cagctggcca
    11101 acctgcccga cctttataag gttttctaac cggtcatcat caccatcacc attgagttta
    11161 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
    11221 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
    11281 gaaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
    11341 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
    11401 tatggcttct gaggcggaaa gaaccagctg gggctcgata ccgtcgacct ctagctagag
    11461 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
    11521 acacaacata cgagccggaa gcataaagtg taaagcctag ggtgcctaat gagtgagcta
    11581 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
    11641 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
    11701 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
    11761 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
    11821 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
    11881 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
    11941 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
    12001 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
    12061 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctc
//
