LOCUS       sequence-417788-       10111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast 2µ vector for expression of Suc2SP-C99-sfGFP-GAL4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10111)
  AUTHORS   Ortner M, Guschtschin-Schmidt N, Stelzer W, Muhle-Goll C, Langosch D
  TITLE     Permissive Conformations of a Transmembrane Helix Allow 
            Intramembrane Proteolysis by gamma-Secretase.
  JOURNAL   J Mol Biol. 2023 Aug 1;435(18):168218. doi: 
            10.1016/j.jmb.2023.168218.
  PUBMED    37536392
REFERENCE   2  (bases 1 to 10111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2023-08-1"; volume: "435(18):168218. doi"; pages: " 
            10.1016/j.jmb.2023.168218"
COMMENT     Sequence Label: pYea5.1_C99_sfGFP
FEATURES             Location/Qualifiers
     source          1..10111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..373
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1470..4112
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4"
                     /product="GAL4 transcriptional activator"
                     /label=GAL4
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF
                     MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN
                     RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI
                     LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ
                     KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS
                     IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA
                     KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL
                     RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW
                     NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI
                     QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY
                     VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL
                     QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             4119..4142
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     terminator      4146..4193
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4220..4407
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4275..4299)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4431..4447)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4431..4447)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4444..4466)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4455..4471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4479..4485,4486..4503,4504..4509))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4524..4545
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4662..4679)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4833..5421)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4913..4932)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5750..5797
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5822..5841
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6029..6823)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6029..6823)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6140..6159)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6750..6769
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6824..6928)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6955..8297
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(7918..7965)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     8338..8356
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8394..8416)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8516..8535
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8556..8837
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             8838..9512
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(9615..10070)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9752..9773)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9964..9983
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccagg acatggaggc ccagaatacc ctccttgaca
       61 gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt tagcccatac
      121 atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg cgaagcaaaa
      181 attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca gacgcgttga
      241 attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg ccactgaggt
      301 tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac atcacatccg
      361 aacataaaca accaagcttg aagctagcct cctgaaagat gcttttgcaa gcattccttt
      421 tccttttggc tggttttgca gccaagatct ctgccatgga cgcagaattc aggcacgact
      481 caggatacga ggtgcaccac cagaagctag tattcttcgc agaggacgta ggaagtaaca
      541 agggagcaat cataggctta atggtaggcg gcgtagtcat cgcaacagtg atcgtcataa
      601 cactggtcat gttaaagaag aagcagtaca catcgataca ccacggagtg gtggaagtgg
      661 acgccgccgt gacacctgag gagcgtcacc ttagcaagat gcagcagaac ggatacgaga
      721 acccgaccta caagttcttc gagcagatgc agaacggtac catgtcaaaa ggtgaagaat
      781 tatttacagg tgtagtccct attctagtgg agttagatgg agatgtcaat ggacataagt
      841 tttccgtcag gggagaggga gagggcgatg ccacaaatgg aaagttaaca ttgaagttta
      901 tttgtacaac ggggaagtta cctgtgcctt ggcctacatt ggtaacaact ttgacgtatg
      961 gagtccaatg tttctcaagg tatccagatc atatgaagca acatgatttc tttaagtcgg
     1021 cgatgcctga gggctatgta caagagagaa ctatttcctt taaggatgat ggtacttata
     1081 agacgagagc agaggtgaag tttgagggtg atacgcttgt caataggatt gagttgaagg
     1141 ggatagattt taaggaagat ggcaatatcc tgggccataa gttggagtac aactttaatt
     1201 cccataatgt ctatattact gcggataagc agaagaatgg aattaaggca aatttcaaga
     1261 ttaggcataa tgtagaggat gggagtgtac aactagcaga tcattatcaa caaaatacac
     1321 ctataggcga tggaccagtc ttactaccag ataatcatta tttatccaca caaagtgtct
     1381 tatccaagga ccctaatgag aagagagatc atatggtcct attagagttc gtgacggcag
     1441 caggaattac gcatggtatg gatgaactaa tgaagctact gagctctatc gaacaagcat
     1501 gcgatatttg ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag tgcgccaagt
     1561 gtctgaagaa caactgggag tgtcgctact ctcccaaaac caaaaggtct ccgctgacta
     1621 gggcacatct gacagaagtg gaatcaaggc tagaaagact ggaacagcta tttctactga
     1681 tttttcctcg agaagacctt gacatgattt tgaaaatgga ttctttacag gatataaaag
     1741 cattgttaac aggattattt gtacaagata atgtgaataa agatgccgtc acagatagat
     1801 tggcttcagt ggagactgat atgcctctaa cattgagaca gcatagaata agtgcgacat
     1861 catcatcgga agagagtagt aacaaaggtc aaagacagtt gactgtatcg attgactcgg
     1921 cagctcatca tgataactcc acaattccgt tggattttat gcccagggat gctcttcatg
     1981 gatttgattg gtctgaagag gatgacatgt cggatggctt gcccttcctg aaaacggacc
     2041 ccaacaataa tgggttcttt ggcgacggtt ctctcttatg tattcttcga tctattggct
     2101 ttaaaccgga aaattacacg aactctaacg ttaacaggct cccgaccatg attacggata
     2161 gatacacgtt ggcttctaga tccacaacat cccgtttact tcaaagttat ctcaataatt
     2221 ttcaccccta ctgccctatc gtgcactcac cgacgctaat gatgttgtat aataaccaga
     2281 ttgaaatcgc gtcgaaggat caatggcaaa tcctttttaa ctgcatatta gccattggag
     2341 cctggtgtat agagggggaa tctactgata tagatgtttt ttactatcaa aatgctaaat
     2401 ctcatttgac gagcaaggtc ttcgagtcag gttccataat tttggtgaca gccctacatc
     2461 ttctgtcgcg atatacacag tggaggcaga aaacaaatac tagctataat tttcacagct
     2521 tttccataag aatggccata tcattgggct tgaataggga cctcccctcg tccttcagtg
     2581 atagcagcat tctggaacaa agacgccgaa tttggtggtc tgtctactct tgggagatcc
     2641 aattgtccct gctttatggt cgatccatcc agctttctca gaatacaatc tccttccctt
     2701 cttctgtcga cgatgtgcag cgtaccacaa caggtcccac catatatcat ggcatcattg
     2761 aaacagcaag gctcttacaa gttttcacaa aaatctatga actagacaaa acagtaactg
     2821 cagaaaaaag tcctatatgt gcaaaaaaat gcttgatgat ttgtaatgag attgaggagg
     2881 tttcgagaca ggcaccaaag tttttacaaa tggatatttc caccaccgct ctaaccaatt
     2941 tgttgaagga acacccttgg ctatccttta caagattcga actgaagtgg aaacagttgt
     3001 ctcttatcat ttatgtatta agagattttt tcactaattt tacccagaaa aagtcacaac
     3061 tagaacagga tcaaaatgat catcaaagtt atgaagttaa acgatgctcc atcatgttaa
     3121 gcgatgcagc acaaagaact gttatgtctg taagtagcta tatggacaat cataatgtca
     3181 ccccatattt tgcctggaat tgttcttatt acttgttcaa tgcagtccta gtacccataa
     3241 agactctact ctcaaactca aaatcgaatg ctgagaataa cgagaccgca caattattac
     3301 aacaaattaa cactgttctg atgctattaa aaaaactggc cacttttaaa atccagactt
     3361 gtgaaaaata cattcaagta ctggaagagg tatgtgcgcc gtttctgtta tcacagtgtg
     3421 caatcccatt accgcatatc agttataaca atagtaatgg tagcgccatt aaaaatattg
     3481 tcggttctgc aactatcgcc caatacccta ctcttccgga ggaaaatgtc aacaatatca
     3541 gtgttaaata tgtttctcct ggctcagtag ggccttcacc tgtgccattg aaatcaggag
     3601 caagtttcag tgatctagtc aagctgttat ctaaccgtcc accctctcgt aactctccag
     3661 tgacaatacc aagaagcaca ccttcgcatc gctcagtcac gccttttcta gggcaacagc
     3721 aacagctgca atcattagtg ccactgaccc cgtctgcttt gtttggtggc gccaatttta
     3781 atcaaagtgg gaatattgct gatagctcat tgtccttcac tttcactaac agtagcaacg
     3841 gtccgaacct cataacaact caaacaaatt ctcaagcgct ttcacaacca attgcctcct
     3901 ctaacgttca tgataacttc atgaataatg aaatcacggc tagtaaaatt gatgatggta
     3961 ataattcaaa accactgtca cctggttgga cggaccaaac tgcgtataac gcgtttggaa
     4021 tcactacagg gatgtttaat accactacaa tggatgatgt atataactat ctattcgatg
     4081 atgaagatac cccaccaaac ccaaaaaaag agtccgcatg gagtcatccg caattcgaaa
     4141 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgcgcgctt
     4201 gcagccaagc taattccggg cgaatttctt atgatttatg atttttatta ttaaataagt
     4261 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt
     4321 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt
     4381 cgctcttatt gaccacacct ctaccggcat gcaagcttgg cgtaatcatg gtcatagctg
     4441 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     4501 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     4561 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     4621 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     4681 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4741 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4801 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4861 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4921 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4981 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5041 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5101 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5161 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5221 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     5281 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5341 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5401 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5461 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5521 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaacct
     5581 gaggctatgg cagggcctgc cgccccgacg ttggctgcga gccctgggcc ttcacccgaa
     5641 cttggggggt ggggtgggga aaaggaagaa acgcgggcgt attggcccca atggggtctc
     5701 ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac
     5761 ccaacaccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt
     5821 attgtctcct tccgtgtttc agttagcctc cccctagggt gggcgaagaa ctccagcatg
     5881 agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac
     5941 ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg
     6001 tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
     6061 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     6121 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     6181 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     6241 tcggcaagca ggcatcgcca tgagtcacga cgagatcctc gccgtcgggc atgctcgcct
     6301 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     6361 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     6421 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     6481 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     6541 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     6601 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac
     6661 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     6721 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     6781 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catactcttc ctttttcaat
     6841 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6901 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaacgaa
     6961 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     7021 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
     7081 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc
     7141 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt
     7201 accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt
     7261 tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct
     7321 cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta
     7381 ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag
     7441 ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat
     7501 tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt
     7561 atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg
     7621 tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa
     7681 tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa
     7741 aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt
     7801 tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc
     7861 gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa
     7921 gttcctatac tttctagaga ataggaactt cggaatagga acttcaaagc gtttccgaaa
     7981 acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt tcacgtcgca
     8041 cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat agtgcgtgtt
     8101 tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta gtctagtacc
     8161 tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc actacccttt
     8221 agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa tgctatcatt
     8281 tcctttgata ttggatcata ctaagaaacc attattatca tgacattaac ctataaaaat
     8341 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     8401 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     8461 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     8521 tcagagcaga ttgtactgag agtgcaccat agatcaacga cattactata tatataatat
     8581 aggaagcatt taatagaaca gcatcgtaat atatgtgtac tttgcagtta tgacgccaga
     8641 tggcagtagt ggaagatatt ctttattgaa aaatagcttg tcaccttacg tacaatcttg
     8701 atccggagct tttctttttt tgccgattaa gaattaattc ggtcgaaaaa agaaaaggag
     8761 agggccaaga gggagggcat tggtgactat tgagcacgtg agtatacgtg attaagcaca
     8821 caaaggcagc ttggagtatg tctgttatta atttcacagg tagttctggt ccattggtga
     8881 aagtttgcgg cttgcagagc acagaggccg cagaatgtgc tctagattcc gatgctgact
     8941 tgctgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt attgcaagga
     9001 aaatttcaag tcttgtaaaa gcatataaaa atagttcagg cactccgaaa tacttggttg
     9061 gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat tacggcattg
     9121 atatcgtcca actgcatgga gatgagtcgt ggcaagaata ccaagagttc ctcggtttgc
     9181 cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt gcagcttcac
     9241 agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca ggtgaacttt
     9301 tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgaa agcttacatt
     9361 ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg
     9421 ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa
     9481 tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt
     9541 attgtttgtg cacttgccga tctatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     9601 aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     9661 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     9721 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     9781 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     9841 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     9901 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     9961 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
    10021 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca
    10081 ttcaggctgc gcaactgttg ggaagggcga t
//
