LOCUS       sequence-417817-       11169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDEST vector to tag gene of interest with mEGFP on the C-terminus to
            perform localization assays in S. cerevisiae. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Evans-Yamamoto D, Dube AK, Saha G, Plante S, Bradley D, 
            Gagnon-Arsenault I, Landry CR
  TITLE     Parallel nonfunctionalization of CK1delta/epsilon kinase ohnologs 
            following a whole-genome duplication event.
  JOURNAL   Mol Biol Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246.
  PUBMED    37979156
REFERENCE   2  (bases 1 to 11169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246."
COMMENT     Sequence Label: pDN0617 (pDEST-mEGFP C-term, NatR)
FEATURES             Location/Qualifiers
     source          1..11169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    864..988
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1162..1264
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1265..1945
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(1331..1350)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2265..2570
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2449..2468
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2611..2735)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3720..3907
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4059..4078)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4060..4078)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4085..4102)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4085..4101)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4094..4116)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4242..4697
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4329..4348)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4539..4560
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4836..5179
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5180..5752
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      5760..5947
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6099..6118)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6218..6240
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6278..6296)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6337..6840
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6877..6981
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6982..7050,7051..7842)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7200..7219)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8013..8601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8502..8521
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8755..8772
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8889..8910
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8925..8930,8931..8948,8949..8955)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8963..8979
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8968..8990
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8987..9003
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8987..9003
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9024..9042
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      10260..10507
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(10276..10294)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 actttacact tctcctatgc acatatatta atgtaactat aacggtccta aggtagcgaa
       61 attaaagtcc aatgctagta gagaaggggg gtaacacccc tccgcgctct tttccgattt
      121 ttttctaaac cgtggaatat ttcggatatc cttttgttgt ttccgggtgt acaatatgga
      181 cttcctcttt tctggcaacc aaacccatac atcgggattc ctataatacc ttcgttggtc
      241 tccctaacat gtaggtggcg gaggggagat atacaataga acagatacca gacaagacat
      301 aatgggctaa acaagactac accaattaca ctgcctcatt gatggtggta cataacgaac
      361 taatactgta gccctagact tgatagccat catcatatcg aagtttcact accctttttc
      421 catttgccat ctattgaagt aataataggc gcatgcaact tcttttcttt ttttttcttt
      481 tctctctccc ccgttgttgt ctcaccatat ccgcaatgac aaaaaaatga tggaagacac
      541 taaaggaaaa aattaacgac aaagacagca ccaacagatg tcgttgttcc agagctgatg
      601 aggggtatct cgaagcacac gaaacttttt ccttccttca ttcacgcaca ctactctcta
      661 atgagcaacg gtatacggcc ttccttccag ttacttgaat ttgaaataaa aaaaagtttg
      721 ctgtcttgct atcaagtata aatagacctg caattattaa tcttttgttt cctcgtcatt
      781 gttctcgttc cctttcttcc ttgtttcttt ttctgcacaa tatttcaagc tataccaagc
      841 atacaaaggg tgggtcgaat caaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
      901 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
      961 gtaaaacaca acatatccag tcactatggc ggccgcgggt gatgctgcca acttagcggc
     1021 cgctaagttg gcagcatcac ccgacgcact ttgcgccgaa taaatacctg tgacggaaga
     1081 tcacttcgca gaataaataa atcctggtgt ccctgttgat accgggaagc cctgggccaa
     1141 cttttggcga aaatgagacg ttgatcggca cgtaagaggt tccaactttc accataatga
     1201 aataagatca ctaccgggcg tattttttga gtcatcgaga ttttcaggag ctaaggaagc
     1261 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     1321 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     1381 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     1441 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     1501 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     1561 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacatat
     1621 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     1681 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     1741 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     1801 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     1861 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc aggcggggcg
     1921 taatctagag gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
     1981 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg
     2041 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
     2101 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
     2161 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
     2221 tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt
     2281 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     2341 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     2401 tcccccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     2461 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     2521 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     2581 cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag
     2641 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     2701 tacgtttctc gttcagcttt cttgtacaaa gtggtaggag gaggaagtac tatggtttcc
     2761 aagggtgaag aactattcac tggtgtcgtc ccaatcttgg tcgaattgga cggtgacgtc
     2821 aacggtcaca agttttcagt ctccggtgaa ggtgaaggtg acgctaccta cggtaaattg
     2881 actttgaagt tcatctgtac cacaggtaaa ttgcccgtcc catggccaac tttagtcact
     2941 actttaactt acggtgtcca atgtttcagt cgttacccag accacatgaa gcaacacgac
     3001 ttcttcaagt ctgccatgcc agaaggttac gttcaagaaa gaaccatctt cttcaaggat
     3061 gatggtaact acaaaactag agctgaagtt aagttcgaag gtgatacttt ggtcaacaga
     3121 attgaattga aaggtattga tttcaaggaa gatggtaaca ttttgggtca caagttggaa
     3181 tataattaca actctcacaa tgtgtacatc atggcagata aacaaaagaa cggtatcaag
     3241 gttaacttca agattagaca caacatcgaa gatggttccg tccaattggc tgatcattac
     3301 caacaaaaca ctccaatcgg tgacggtcca gtcttgttgc cagataacca ctacctatct
     3361 acccaaagta agttgagcaa ggaccctaat gaaaagagag atcacatggt tttattggaa
     3421 ttcgttaccg ctgctggtat tactcatggt atggacgaat tgtacaagta aagtttaatt
     3481 aagagctctt aagtaagtaa tggcaaacag ctattatggg tattatgggt tagggataac
     3541 agggtaataa gctctaagta agtaacggcc gccaccgcgg tggagctttg gacttcttcg
     3601 ccagaggttt ggtcaagtct ccaatcaagg ttgtcggctt gtctaccttg ccagaaattt
     3661 acgaaaagat ggaaaagggt caaatcgttg gtagatacgt tgttgacact tctaaataag
     3721 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat
     3781 acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt
     3841 tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct
     3901 ctaccggcat gccgagcaaa tgcctgcaaa tcgctcccca tttcacccaa ttgtagatat
     3961 gctaactcca gcaatgagtt gatgaatctc ggtgtgtatt ttatgtcctc agaggacaac
     4021 acctgttgta atcgttcttc cacacggatc ccaattcgcc ctatagtgag tcgtattaca
     4081 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4141 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4201 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc
     4261 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4321 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4381 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4441 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4501 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4561 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4621 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4681 attaacgctt acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4741 caccgcgtac gctgcaggtc gacggatccc cgggttaatt aaggcgcgcc agatctgttt
     4801 agcttgcctc gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc
     4861 ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc
     4921 ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa
     4981 gcaaaaatta cggctcctcg ctgcagacct gcgagcaggg aaacgctccc ctcacagacg
     5041 cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac
     5101 tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca
     5161 catccgaaca taaacaacca tgggtaccac tcttgacgac acggcttacc ggtaccgcac
     5221 cagtgtcccg ggggacgccg aggccatcga ggcactggat gggtccttca ccaccgacac
     5281 cgtcttccgc gtcaccgcca ccggggacgg cttcaccctg cgggaggtgc cggtggaccc
     5341 gcccctgacc aaggtgttcc ccgacgacga atcggacgac gaatcggacg acggggagga
     5401 cggcgacccg gactcccgga cgttcgtcgc gtacggggac gacggcgacc tggcgggctt
     5461 cgtggtcgtc tcgtactccg gctggaaccg ccggctgacc gtcgaggaca tcgaggtcgc
     5521 cccggagcac cgggggcacg gggtcgggcg cgcgttgatg gggctcgcga cggagttcgc
     5581 ccgcgagcgg ggcgccgggc acctctggct ggaggtcacc aacgtcaacg caccggcgat
     5641 ccacgcgtac cggcggatgg ggttcaccct ctgcggcctg gacaccgccc tgtacgacgg
     5701 caccgcctcg gacggcgagc aggcgctcta catgagcatg ccctgcccct aatctcgagg
     5761 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat
     5821 acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt
     5881 tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct
     5941 ctaccggcat gccgagcaaa tgcctgcaaa tcgctcccca tttcacccaa ttgtagatat
     6001 gctaactcca gcaatgagtt gatgaatctc ggtgtgtatt ttatgtcctc agaggacaac
     6061 acctgttgta atcgagctcg aattcatcga tctctcagta caatctgctc tgatgccgca
     6121 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     6181 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     6241 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     6301 taggttaatg tcatgataat aatggtttct tagacggatc gcttgcctgt aacttacacg
     6361 cgcctcgtat cttttaatga tggaataatt tgggaattta ctctgtgttt atttattttt
     6421 atgttttgta tttggatttt agaaagtaaa taaagaaggt agaagagtta cggaatgaag
     6481 aaaaaaaaat aaacaaaggt ttaaaaaatt tcaacaaaaa gcgtacttta catatatatt
     6541 tattagacaa gaaaagcaga ttaaatagat atacattcga ttaacgataa gtaaaatgta
     6601 aaatcacagg attttcgtgt gtggtcttct acacagacaa gatgaaacaa ttcggcatta
     6661 atacctgaga gcaggaagag caagataaaa ggtagtattt gttggcgatc cccctagagt
     6721 cttttacatc ttcggaaaac aaaaactatt ttttctttaa tttctttttt tactttctat
     6781 ttttaattta tatatttata ttaaaaaatt taaattataa ttatttttat agcacgtgat
     6841 gaaaaggacc caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6901 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6961 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     7021 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     7081 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     7141 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     7201 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     7261 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     7321 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7381 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7441 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7501 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     7561 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7621 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7681 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7741 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7801 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7861 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7921 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7981 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8041 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8101 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     8161 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8221 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     8281 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8341 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8401 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8461 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8521 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8581 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8641 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     8701 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     8761 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     8821 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     8881 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     8941 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     9001 gaccatgatt acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctgggatcc
     9061 ccagattcta tttgcagttg ccaatcctgt atctcttgtg cggctccatc aaaagtcacg
     9121 ccacgtagct tacatagaga taacttagca tcggtgactc ttccatgtgc taacaaccat
     9181 tttggagatt catcgaaaaa cacagcaaaa acaagagtac tgtaaccaat gtaacatctg
     9241 tacaccaggg acccacacat taccaaaatc aaaattattt ttctaatgcc tgttattttt
     9301 cctatttttc ctctggcgcg tgaatagccc gcagagacgc aaacaatttt cctcgcagtt
     9361 tttcgcttgt ttaatgcgta ttttcccaga taggttcaaa cctttcatct gtatcccgta
     9421 tatttaagat ggcgtttgct ttctccgttg atttttttcc ttcttagtga tttttttgca
     9481 ttaaatccca gaacaatcat ccaactaatc aagaatgcct tccagattca ctaagactag
     9541 aaagcacaga ggtcacgtct cagccggtaa aggtcgtatc ggtaagcaca gaaagcaccc
     9601 cggtggtaga ggtatggccg gtggtcaaca tcaccacaga attaacatgg ataaatacca
     9661 tccaggttat ttcggtaagg ttggtatgag atacttccac aagcaacaag ctcatttctg
     9721 gaagccagtc ttgaacttgg acaaattgtg gacattgatc ccagaagaca agagagacca
     9781 atacttgaaa tctgcttcta aggaaactgc tccagttatt gacactttgg cagccggtta
     9841 cggtaagatc ttgggtaagg gtagaattcc aaatgttcca gttatcgtca aagctagatt
     9901 cgtctccaag ttggctgaag aaaaaatcag agctgctggt ggtgttgttg aattgatcgc
     9961 ttaagcgcat caacaaaaac tctatgtatt ttccaataaa ttatatatct tcagtttaat
    10021 ctaattcaac atctacttct gtattatttc tatcaaaaaa aaaaaaaaaa aaaaaaaaaa
    10081 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
    10141 aaaaaaaaaa agtaccaaga tggcctttgg tgggttgaag aaggaaaaag acagaaacga
    10201 cttaattacc tacttgaaaa aagcctgtga gtaaacaggc cccttttcct ttgtcgatat
    10261 catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga
    10321 aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt
    10381 agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta
    10441 cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt
    10501 aatttgcaag ctcgggatcc gggccccccc tcgagatccg ggatcgaaga aatgatggta
    10561 aatgaaatag gaaatcaagg agcatgaagg caaaagacaa atataagggt cgaacgaaaa
    10621 ataaagtgaa aagtgttgat atgatgtatt tggctttgcg gcgccgaaaa aacgagttta
    10681 cgcaattgca caatcatgct gactctgtgg cggacccgcg ctcttgccgg cccggcgata
    10741 acgctgggcg tgaggctgtg cccggcggag ttttttgcgc ctgcattttc caaggtttac
    10801 cctgcgctaa ggggcgagat tggagaagca ataagaatgc cggttggggt tgcgatgatg
    10861 acgaccacga caactggtgt cattatttaa gttgccgaaa gaacctgagt gcatttgcaa
    10921 catgagtata ctagaagaat gagccaagac ttgcgagacg cgagtttgcc ggtggtgcga
    10981 acaatagagc gaccatgacc ttgaaggtga gacgcgcata accgctagag tactttgaag
    11041 aggaaacagc aatagggttg ctaccagtat aaatagacag gtacatacaa cactggaaat
    11101 ggttgtctgt ttgagtacgc tttcaattca tttgggtgtg cactttatta tgttacaata
    11161 tggaaggga
//
