LOCUS       sequence-417818-       10710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDEST vector to tag gene of interest with DHFR F[1,2] on the 
            C-terminus to perform DHFR-PCA in S. cerevisiae. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Evans-Yamamoto D, Dube AK, Saha G, Plante S, Bradley D, 
            Gagnon-Arsenault I, Landry CR
  TITLE     Parallel nonfunctionalization of CK1delta/epsilon kinase ohnologs 
            following a whole-genome duplication event.
  JOURNAL   Mol Biol Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246.
  PUBMED    37979156
REFERENCE   2  (bases 1 to 10710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246."
COMMENT     Sequence Label: pKB11 (pDEST-DHFR F[1,2] C-term, NatR)
FEATURES             Location/Qualifiers
     source          1..10710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    859..983
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1157..1259
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1260..1940
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(1326..1345)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2260..2565
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2444..2463
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2606..2730)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3309..3496
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3648..3667)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3649..3667)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3674..3691)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3674..3690)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3683..3705)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3831..4286
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3918..3937)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4128..4149
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4425..4768
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             4769..5341
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     terminator      5349..5536
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5688..5707)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5807..5829
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5867..5885)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    5926..6429
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6466..6570
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6571..6639,6640..7431)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6789..6808)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7602..8190
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8091..8110
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8344..8361
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8478..8499
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8514..8519,8520..8537,8538..8544)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8552..8568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8557..8579
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8576..8592
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8576..8592
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8613..8631
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      9796..10043
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(9812..9830)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 acacttctcc tatgcacata tattaatgta actataacgg tcctaaggta gcgaaattaa
       61 agtccaatgc tagtagagaa ggggggtaac acccctccgc gctcttttcc gatttttttc
      121 taaaccgtgg aatatttcgg atatcctttt gttgtttccg ggtgtacaat atggacttcc
      181 tcttttctgg caaccaaacc catacatcgg gattcctata ataccttcgt tggtctccct
      241 aacatgtagg tggcggaggg gagatataca atagaacaga taccagacaa gacataatgg
      301 gctaaacaag actacaccaa ttacactgcc tcattgatgg tggtacataa cgaactaata
      361 ctgtagccct agacttgata gccatcatca tatcgaagtt tcactaccct ttttccattt
      421 gccatctatt gaagtaataa taggcgcatg caacttcttt tctttttttt tcttttctct
      481 ctcccccgtt gttgtctcac catatccgca atgacaaaaa aatgatggaa gacactaaag
      541 gaaaaaatta acgacaaaga cagcaccaac agatgtcgtt gttccagagc tgatgagggg
      601 tatctcgaag cacacgaaac tttttccttc cttcattcac gcacactact ctctaatgag
      661 caacggtata cggccttcct tccagttact tgaatttgaa ataaaaaaaa gtttgctgtc
      721 ttgctatcaa gtataaatag acctgcaatt attaatcttt tgtttcctcg tcattgttct
      781 cgttcccttt cttccttgtt tctttttctg cacaatattt caagctatac caagcataca
      841 aagggtgggt cgaatcaaac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat
      901 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
      961 acacaacata tccagtcact atggcggccg cgggtgatgc tgccaactta gcggccgcta
     1021 agttggcagc atcacccgac gcactttgcg ccgaataaat acctgtgacg gaagatcact
     1081 tcgcagaata aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt
     1141 ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
     1201 gatcactacc gggcgtattt tttgagtcat cgagattttc aggagctaag gaagctaaaa
     1261 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     1321 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     1381 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     1441 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     1501 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     1561 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta catatatatt
     1621 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     1681 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     1741 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     1801 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     1861 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcaggcg gggcgtaatc
     1921 tagaggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg
     1981 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg
     2041 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
     2101 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
     2161 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
     2221 tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc
     2281 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
     2341 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtcccc
     2401 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
     2461 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
     2521 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt
     2581 atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta
     2641 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
     2701 ttctcgttca gctttcttgt acaaagtggt aggaggagga agtactagta tggttcgacc
     2761 attgaactgc atcgtcgccg tgtcccaaaa tatggggatt ggcaagaacg gagactatcc
     2821 ctggcctccg ctcaggaacg agtccaagta cttccaaaga atgaccacaa cctcttcagt
     2881 ggaaggtaaa cagaatctgg tgattatggg taggaaaacc tggttctcca ttcctgagaa
     2941 gaatcgacct ttaaaggaca gaattaatat agttctcagt agagaactca aagaaccacc
     3001 acgaggagct cattttcttg ccaaaagttt ggatgatgcc ttaagactta ttgaacaacc
     3061 ggaattgggt acctaattta agtaagtaat ggcaaacagc tattatgggt attatgggtt
     3121 agggataaca gggtaataag ctctaagtaa gtaacggccg ccaccgcggt ggagctttgg
     3181 acttcttcgc cagaggtttg gtcaagtctc caatcaaggt tgtcggcttg tctaccttgc
     3241 cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg tagatacgtt gttgacactt
     3301 ctaaataagc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa
     3361 taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga
     3421 gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg
     3481 accacacctc taccggcatg ccgagcaaat gcctgcaaat cgctccccat ttcacccaat
     3541 tgtagatatg ctaactccag caatgagttg atgaatctcg gtgtgtattt tatgtcctca
     3601 gaggacaaca cctgttgtaa tcgttcttcc acacggatcc caattcgccc tatagtgagt
     3661 cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3721 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     3781 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg
     3841 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3901 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3961 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4021 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4081 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4141 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4201 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4261 taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc
     4321 ggtatttcac accgcgtacg ctgcaggtcg acggatcccc gggttaatta aggcgcgcca
     4381 gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagcgacatg gaggcccaga
     4441 ataccctcct tgacagtctt gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt
     4501 acatttagcc catacatccc catgtataat catttgcatc catacatttt gatggccgca
     4561 cggcgcgaag caaaaattac ggctcctcgc tgcagacctg cgagcaggga aacgctcccc
     4621 tcacagacgc gttgaattgt ccccacgccg cgcccctgta gagaaatata aaaggttagg
     4681 atttgccact gaggttcttc tttcatatac ttccttttaa aatcttgcta ggatacagtt
     4741 ctcacatcac atccgaacat aaacaaccat gggtaccact cttgacgaca cggcttaccg
     4801 gtaccgcacc agtgtcccgg gggacgccga ggccatcgag gcactggatg ggtccttcac
     4861 caccgacacc gtcttccgcg tcaccgccac cggggacggc ttcaccctgc gggaggtgcc
     4921 ggtggacccg cccctgacca aggtgttccc cgacgacgaa tcggacgacg aatcggacga
     4981 cggggaggac ggcgacccgg actcccggac gttcgtcgcg tacggggacg acggcgacct
     5041 ggcgggcttc gtggtcgtct cgtactccgg ctggaaccgc cggctgaccg tcgaggacat
     5101 cgaggtcgcc ccggagcacc gggggcacgg ggtcgggcgc gcgttgatgg ggctcgcgac
     5161 ggagttcgcc cgcgagcggg gcgccgggca cctctggctg gaggtcacca acgtcaacgc
     5221 accggcgatc cacgcgtacc ggcggatggg gttcaccctc tgcggcctgg acaccgccct
     5281 gtacgacggc accgcctcgg acggcgagca ggcgctctac atgagcatgc cctgccccta
     5341 atctcgaggc gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa
     5401 taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga
     5461 gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg
     5521 accacacctc taccggcatg ccgagcaaat gcctgcaaat cgctccccat ttcacccaat
     5581 tgtagatatg ctaactccag caatgagttg atgaatctcg gtgtgtattt tatgtcctca
     5641 gaggacaaca cctgttgtaa tcgagctcga attcatcgat ctctcagtac aatctgctct
     5701 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     5761 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     5821 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     5881 ctatttttat aggttaatgt catgataata atggtttctt agacggatcg cttgcctgta
     5941 acttacacgc gcctcgtatc ttttaatgat ggaataattt gggaatttac tctgtgttta
     6001 tttattttta tgttttgtat ttggatttta gaaagtaaat aaagaaggta gaagagttac
     6061 ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag cgtactttac
     6121 atatatattt attagacaag aaaagcagat taaatagata tacattcgat taacgataag
     6181 taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta cacagacaag atgaaacaat
     6241 tcggcattaa tacctgagag caggaagagc aagataaaag gtagtatttg ttggcgatcc
     6301 ccctagagtc ttttacatct tcggaaaaca aaaactattt tttctttaat ttcttttttt
     6361 actttctatt tttaatttat atatttatat taaaaaattt aaattataat tatttttata
     6421 gcacgtgatg aaaaggaccc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6481 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6541 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6601 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6661 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6721 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6781 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6841 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6901 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6961 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7021 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     7081 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     7141 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7201 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7261 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7321 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7381 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7441 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7501 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7561 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7621 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7681 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7741 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7801 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7861 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7921 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7981 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8041 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8101 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8161 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8221 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8281 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8341 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     8401 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     8461 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     8521 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     8581 aacagctatg accatgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag
     8641 ctgggatccc cagattctat ttgcagttgc caatcctgta tctcttgtgc ggctccatca
     8701 aaagtcacgc cacgtagctt acatagagat aacttagcat cggtgactct tccatgtgct
     8761 aacaaccatt ttggagattc atcgaaaaac acagcaaaaa caagagtact gtaaccaatg
     8821 taacatctgt acaccaggga cccacacatt accaaaatca aaattatttt tctaatgcct
     8881 gttatttttc ctatttttcc tctggcgcgt gaatagcccg cagagacgca aacaattttc
     8941 ctcgcagttt ttcgcttgtt taatgcgtat tttcccagat aggttcaaac ctttcatctg
     9001 tatcccgtat atttaagatg gcgtttgctt tctccgttga tttttttcct tcttagtgat
     9061 ttttttgcat taaatcccag aacaatcatc caactaatca agaatgcctt ccagattcac
     9121 taagactaga aagcacagag gtcacgtctc agccggtaaa ggtcgtatcg gtaagcacag
     9181 aaagcacccc ggtggtagag gtatggccgg tggtcaacat caccacagaa ttaacatgga
     9241 taaataccat ccaggttatt tcggtaaggt tggtatgaga tacttccaca agcaacaagc
     9301 tcatttctgg aagccagtct tgaacttgga caaattgtgg acattgatcc cagaagacaa
     9361 gagagaccaa tacttgaaat ctgcttctaa ggaaactgct ccagttattg acactttggc
     9421 agccggttac ggtaagatct tgggtaaggg tagaattcca aatgttccag ttatcgtcaa
     9481 agctagattc gtctccaagt tggctgaaga aaaaatcaga gctgctggtg gtgttgttga
     9541 attgatcgct taagcgcatc aacaaaaact ctatgtattt tccaataaat tatatatctt
     9601 cagtttaatc taattcaaca tctacttctg tattatttct atcaaaaaaa aaaaaaaaaa
     9661 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     9721 aaaaaaaaaa aaaaaaaaaa aaaaacaaaa aaaaaaaaaa aaaaaaaaaa acaggcccct
     9781 tttcctttgt cgatatcatg taattagtta tgtcacgctt acattcacgc cctcccccca
     9841 catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt
     9901 tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc
     9961 tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg
    10021 gacgctcgaa ggctttaatt tgcaagctcg ggatccgggc cccccctcga gatccgggat
    10081 cgaagaaatg atggtaaatg aaataggaaa tcaaggagca tgaaggcaaa agacaaatat
    10141 aagggtcgaa cgaaaaataa agtgaaaagt gttgatatga tgtatttggc tttgcggcgc
    10201 cgaaaaaacg agtttacgca attgcacaat catgctgact ctgtggcgga cccgcgctct
    10261 tgccggcccg gcgataacgc tgggcgtgag gctgtgcccg gcggagtttt ttgcgcctgc
    10321 attttccaag gtttaccctg cgctaagggg cgagattgga gaagcaataa gaatgccggt
    10381 tggggttgcg atgatgacga ccacgacaac tggtgtcatt atttaagttg ccgaaagaac
    10441 ctgagtgcat ttgcaacatg agtatactag aagaatgagc caagacttgc gagacgcgag
    10501 tttgccggtg gtgcgaacaa tagagcgacc atgaccttga aggtgagacg cgcataaccg
    10561 ctagagtact ttgaagagga aacagcaata gggttgctac cagtataaat agacaggtac
    10621 atacaacact ggaaatggtt gtctgtttga gtacgctttc aattcatttg ggtgtgcact
    10681 ttattatgtt acaatatgga agggaacttt
//
