LOCUS       sequence-417819-        9602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDEST vector to tag gene of interest with DHFR F[3] on the 
            N-terminus to perform DHFR-PCA in S. cerevisiae..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Evans-Yamamoto D, Dube AK, Saha G, Plante S, Bradley D, 
            Gagnon-Arsenault I, Landry CR
  TITLE     Parallel nonfunctionalization of CK1delta/epsilon kinase ohnologs 
            following a whole-genome duplication event.
  JOURNAL   Mol Biol Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246.
  PUBMED    37979156
REFERENCE   2  (bases 1 to 9602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Evol. 2023 Nov 18:msad246. doi: 10.1093/molbev/msad246."
COMMENT     Sequence Label: pDN0606 (pDEST-DHFR F[3] N-term,HygR)
FEATURES             Location/Qualifiers
     source          1..9602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        150..854
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     protein_bind    1126..1250
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1397..1499
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1500..2180
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(1566..1585)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2500..2805
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2684..2703
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2846..2970)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3229..3416
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3284..3308)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3568..3587)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3569..3587)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3594..3611)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3594..3610)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3603..3625)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3751..4206
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3838..3857)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4048..4069
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4345..4688
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             4689..5717
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             4689..5717
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      5725..5972
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5741..5759)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5994..6013)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6113..6135
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6173..6191)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    6233..6736
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        6773..6877
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6878..6946,6947..7738)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7096..7115)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7909..8497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8398..8417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8651..8668
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8785..8806
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8821..8826,8827..8844,8845..8851)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8859..8875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8864..8886
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8883..8899
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8883..8899
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8920..8938
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gaactttaca cttctcctat gcacatatat taatgtaact ataacggtcc taaggtagcg
       61 aaattaaagt ccaatgctag tagagaaggg gggtaacacc cctccgcgct cttttccgat
      121 ttttttctaa accgtggaat atttcggata tccttttgtt gtttccgggt gtacaatatg
      181 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      241 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      301 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      361 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      421 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      481 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
      541 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
      601 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
      661 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
      721 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      781 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      841 gcatacaatc aactccaagc ttggatgagt aaagtagaca tggtttggat agtcggaggc
      901 agttctgttt accaggaagc catgaatcaa ccaggccacc tcagactctt tgtgacaagg
      961 atcatgcagg aatttgaaag tgacacgttt ttcccagaaa ttgatttggg gaaatataaa
     1021 cttctcccag aatacccagg cgtcctctct gaggtccagg aggaaaaagg catcaagtat
     1081 aagtttgaag tctacgagaa gaaagacgga ggaggaagta ctagtacaag tttgtacaaa
     1141 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     1201 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcta
     1261 agttggcagc atcacccgac gcactttgcg ccgaataaat acctgtgacg gaagatcact
     1321 tcgcagaata aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt
     1381 ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
     1441 gatcactacc gggcgtattt tttgagtcat cgagattttc aggagctaag gaagctaaaa
     1501 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     1561 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     1621 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     1681 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     1741 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     1801 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     1861 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     1921 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     1981 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     2041 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     2101 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcaggcg gggcgtaatc
     2161 tagaggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg
     2221 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg
     2281 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
     2341 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
     2401 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
     2461 tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc
     2521 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
     2581 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtcccc
     2641 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
     2701 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
     2761 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt
     2821 atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta
     2881 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
     2941 ttctcgttca gctttcttgt acaaagtggt ttgatggccg ctaagtaagt aagagcttta
     3001 agtaagtaat ggcaaacagc tattatgggt attatgggtt agggataaca gggtaataag
     3061 ctctaagtaa gtaacggccg ccaccgcggt ggagctttgg acttcttcgc cagaggtttg
     3121 gtcaagtctc caatcaaggt tgtcggcttg tctaccttgc cagaaattta cgaaaagatg
     3181 gaaaagggtc aaatcgttgg tagatacgtt gttgacactt ctaaataagc gaatttctta
     3241 tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa
     3301 agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc
     3361 aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg
     3421 ccgagcaaat gcctgcaaat cgctccccat ttcacccaat tgtagatatg ctaactccag
     3481 caatgagttg atgaatctcg gtgtgtattt tatgtcctca gaggacaaca cctgttgtaa
     3541 tcgttcttcc acacggatcc caattcgccc tatagtgagt cgtattacaa ttcactggcc
     3601 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     3661 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     3721 caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg
     3781 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3841 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3901 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3961 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4021 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4081 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4141 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     4201 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcgtacg
     4261 ctgcaggtcg acggatcccc gggttaatta aggcgcgcca gatctgttta gcttgcctcg
     4321 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
     4381 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
     4441 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
     4501 ggctcctcgc tgcggacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
     4561 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
     4621 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
     4681 aaacaaccat gggtaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg
     4741 aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt
     4801 tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt
     4861 tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag
     4921 tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg
     4981 gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg
     5041 aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg
     5101 gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc
     5161 cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg
     5221 ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg
     5281 cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact
     5341 ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc
     5401 cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg
     5461 caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga
     5521 gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg
     5581 tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct
     5641 ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc
     5701 cgagggcaaa ggaataatct cgagtcatgt aattagttat gtcacgctta cattcacgcc
     5761 ctccccccac atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc
     5821 ctatttattt ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc
     5881 ttttttttct gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga
     5941 aggttttggg acgctcgaag gctttaacga gctcgaattc atcgatctct cagtacaatc
     6001 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     6061 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     6121 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     6181 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagga cggatcgctt
     6241 gcctgtaact tacacgcgcc tcgtatcttt taatgatgga ataatttggg aatttactct
     6301 gtgtttattt atttttatgt tttgtatttg gattttagaa agtaaataaa gaaggtagaa
     6361 gagttacgga atgaagaaaa aaaaataaac aaaggtttaa aaaatttcaa caaaaagcgt
     6421 actttacata tatatttatt agacaagaaa agcagattaa atagatatac attcgattaa
     6481 cgataagtaa aatgtaaaat cacaggattt tcgtgtgtgg tcttctacac agacaagatg
     6541 aaacaattcg gcattaatac ctgagagcag gaagagcaag ataaaaggta gtatttgttg
     6601 gcgatccccc tagagtcttt tacatcttcg gaaaacaaaa actatttttt ctttaatttc
     6661 tttttttact ttctattttt aatttatata tttatattaa aaaatttaaa ttataattat
     6721 ttttatagca cgtgatgaaa aggacccagg tggcactttt cggggaaatg tgcgcggaac
     6781 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6841 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6901 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6961 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7021 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7081 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     7141 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7201 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7261 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7321 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     7381 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7441 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7501 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7561 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7621 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7681 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7741 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7801 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7861 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7921 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7981 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8041 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8101 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8161 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8221 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8281 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8341 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8461 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8521 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8581 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8641 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     8701 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     8761 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     8821 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     8881 cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga
     8941 acaaaagctg gtaccgggcc ccccctcgag atccgggatc gaagaaatga tggtaaatga
     9001 aataggaaat caaggagcat gaaggcaaaa gacaaatata agggtcgaac gaaaaataaa
     9061 gtgaaaagtg ttgatatgat gtatttggct ttgcggcgcc gaaaaaacga gtttacgcaa
     9121 ttgcacaatc atgctgactc tgtggcggac ccgcgctctt gccggcccgg cgataacgct
     9181 gggcgtgagg ctgtgcccgg cggagttttt tgcgcctgca ttttccaagg tttaccctgc
     9241 gctaaggggc gagattggag aagcaataag aatgccggtt ggggttgcga tgatgacgac
     9301 cacgacaact ggtgtcatta tttaagttgc cgaaagaacc tgagtgcatt tgcaacatga
     9361 gtatactaga agaatgagcc aagacttgcg agacgcgagt ttgccggtgg tgcgaacaat
     9421 agagcgacca tgaccttgaa ggtgagacgc gcataaccgc tagagtactt tgaagaggaa
     9481 acagcaatag ggttgctacc agtataaata gacaggtaca tacaacactg gaaatggttg
     9541 tctgtttgag tacgctttca attcatttgg gtgtgcactt tattatgtta caatatggaa
     9601 gg
//
