LOCUS       sequence-417822-        2689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pMuLE L1-L4 entry plasmid containing 2 consensus PGR bindings sites 
            upstream of a minimal 2YBTATA promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Aarts MT, Wagner M, van der Wal T, van Boxtel AL, van Amerongen R
  TITLE     A molecular toolbox to study progesterone receptor signaling.
  JOURNAL   J Mammary Gland Biol Neoplasia. 2023 Nov 29;28(1):24. doi: 
            10.1007/s10911-023-09550-0.
  PUBMED    38019315
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mammary 
            Gland Biol Neoplasia."; date: "2023-11-29"; volume: "28(1):24. doi";
            pages: " 10.1007/s10911-023-09550-0"
COMMENT     Sequence Label: pMuLE (L1-L4) 2X PRE
FEATURES             Location/Qualifiers
     source          1..2689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(137..164)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(256..342)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     391..413
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     405..422
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     406..422
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    438..537
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(673..768)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(799..818)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(800..818)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(823..839)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(823..839)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(941..960)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             952..1761
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1019..1038)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1908..2496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2397..2416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2650..2667
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
       61 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
      121 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
      181 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
      241 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
      301 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta
      361 ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca
      421 gtcttaagct cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
      481 aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggctgga
      541 gacccaagct tggtaccgag ctcggatcct gtacaggatg ttctagctac ggatactgta
      601 caggatgttc tagctacaga tcctctagag ggtatataat gggggccact cgagcatgca
      661 tctagaggca cccaactttt ctatacaaag ttggcattat aagaaagcat tgcttatcaa
      721 tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg
      781 cagggcggcc gcgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg
      841 cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
      901 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
      961 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     1021 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     1081 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     1141 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     1201 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     1261 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     1321 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     1381 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     1441 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     1501 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     1561 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     1621 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     1681 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     1741 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     1801 acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg
     1861 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1921 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     1981 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2041 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2101 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2161 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2221 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2281 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     2341 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     2401 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     2461 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     2521 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     2581 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     2641 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatac
//
