LOCUS       sequence-417832-       11684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal tagged NRF1 for miniTurbo proximity experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11684)
  AUTHORS   LaPak KM, Saeidi S, Bok I, Wamsley NT, Plutzer IB, Bhatt DP, Luo J, 
            Ashrafi G, Ben Major M
  TITLE     Proximity proteomic analysis of the NRF family reveals the 
            Parkinson's disease protein ZNF746/PARIS as a co-complexed repressor
            of NRF2.
  JOURNAL   Sci Signal. 2023 Dec 12;16(815):eadi9018. doi: 
            10.1126/scisignal.adi9018. Epub 2023 Dec 12.
  PUBMED    38085818
REFERENCE   2  (bases 1 to 11684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2023-12-12"; volume: "16(815):eadi9018. doi"; 
            pages: " 10.1126/scisignal.adi9018. Epub 2023 Dec 12"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-NRF1
FEATURES             Location/Qualifiers
     source          1..11684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4170..4190
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4190..5401
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5238..5257
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5405..5425)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5426..5435
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5438..6208
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6215..6256
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6257..6281
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8508..8532)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8562..9150
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8615..8635)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9033..9044)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9164..9663
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9199..9222)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9596..9615
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9684..10283
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9684..9703)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10180..10200
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10421..10654
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10726..10847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10763..10782)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10817..10836
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10887..11022
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11042..11061)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11043..11061)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11071..11088)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11071..11087)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11080..11102)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11229..11657
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11316..11335)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11526..11547
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcac aactttgtat agaaaagttg gcctccgcgc
     4201 cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac
     4261 gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc
     4321 tcataagact cggccttaga accccagtat cagcagaagg acattttagg acgggacttg
     4381 ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc
     4441 cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag
     4501 gacgcgccgg gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg
     4561 tttgtggatc gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt
     4621 tcgtggccgc cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta
     4681 gtctgggtcc gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg
     4741 cggctgttcc cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa
     4801 caaggtgggg ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg
     4861 gaaagctctt attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt
     4921 tgtcactgac tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc
     4981 cgttgggcag tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc
     5041 cgttctgttg gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt
     5101 tctccgtcgc aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc
     5161 ggacctctgg tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac
     5221 ctatcttctt aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt
     5281 gttttgtgaa gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt
     5341 tcagtgttag actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga
     5401 cggccaactt ttgtatacaa agtgggccac catgggaatc ccgctgctga acgctaaaca
     5461 gattctggga cagctggacg gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa
     5521 tcagtacctg ctggatcgaa tcggcgagct gaagagtggg gatgcttgca ttgcagaata
     5581 tcagcaggca gggagaggaa gcagagggag gaaatggttc tctccttttg gagctaacct
     5641 gtacctgagt atgttttggc gcctgaagcg gggaccagca gcaatcggcc tgggcccggt
     5701 catcggaatt gtcatggcag aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa
     5761 atggcccaat gacctgtatc tgcaggatag aaagctggca ggcatcctgg tggagctggc
     5821 cggaataaca ggcgatgctg cacagatcgt cattggcgcc gggattaacg tggctatgag
     5881 gcgcgtggag gaaagcgtgg tcaatcaggg ctggatcaca ctgcaggaag cagggattaa
     5941 cctggacagg aatactctgg ccgctatgct gatccgagag ctgcgggcag ccctggaact
     6001 gttcgagcag gaaggcctgg ctccatatct gtcacggtgg gagaagctgg ataacttcat
     6061 caatagaccc gtgaagctga tcattgggga caaagagatt ttcgggatta gccgggggat
     6121 tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg
     6181 agaaatcagt ctgcggtctg ccgaaaaggc tagcggcaag cccatcccca accccctgct
     6241 gggcctggac agcaccacaa ctttgtacaa aaaagttggc atgctttctc tgaagaaata
     6301 cttaacggaa ggacttctcc agttcaccat tctgctgagt ttgattgggg tacgggtgga
     6361 cgtggatact tacctgacct cacagcttcc cccactccgg gagatcatcc tggggcccag
     6421 ttctgcctat actcagaccc agttccacaa cctgaggaat accttggatg gctatggtat
     6481 ccaccccaag agcatagacc tggacaatta cttcactgcc cggcggctcc tcagtcaggt
     6541 gagggccctg gacaggttcc aggtgccaac cactgaggta aatgcctggc tggttcaccg
     6601 agacccagag gggtctgtct ctggcagtca gcccaactca ggcctcgccc tcgagagttc
     6661 cagtggcctc caagatgtga caggcccaga caacggggtg cgagaaagcg aaacggagca
     6721 gggattcggt gaagatttgg aggatttggg ggctgtagcc cccccagtca gtggagactt
     6781 aaccaaagag gacatagatc tgattgacat cctttggcga caggatattg atctgggggc
     6841 tgggcgtgag gtttttgact atagtcaccg ccagaaggag caggatgtgg agaaggagct
     6901 gcgagatgga ggcgagcagg acacctgggc aggcgagggc gcggaagctc tggcacggaa
     6961 cctgctagtg gatggagaga ctggggagag cttccctgca cagtttccag cagacatttc
     7021 cagcataaca gaagcagtgc ctagtgagag tgagccccct gctcttcaaa acaacctctt
     7081 gtctcctctt ctgaccggga cagagtcacc atttgatttg gaacagcagt ggcaagatct
     7141 catgtccatc atggaaatgc aggccatgga agtgaacaca tcagcaagtg aaatcctgta
     7201 cagtgcccct cctggagacc cactgagcac caactacagc cttgccccca acactcccat
     7261 caatcagaat gtcagcctgc atcaggcgtc cctggggggc tgcagccagg acttcttact
     7321 cttcagcccc gaggtggaaa gcctgcctgt ggccagtagc tccacgctgc tcccgttggc
     7381 ccccagcaat tctaccagcc tcaactccac cttcggctcc accaacctga cagggctctt
     7441 ctttccaccc cagctcaatg gcacagccaa tgacacagca ggcccagagc tgcctgaccc
     7501 tttggggggt ctgttagatg aagctatgtt ggatgagatc agccttatgg acctggccat
     7561 tgaagaaggc tttaaccctg tgcaggcctc ccagctggag gaggaatttg actctgactc
     7621 aggcctttcc ttagactcga gccatagccc ttcttcccta agcagctctg aaggcagttc
     7681 ttcctcttct tcctcctcct cttcctcttc ttcctctgct tcttcctctg cctcttcctc
     7741 cttttctgag gaaggtgcgg ttggctacag ctctgactct gagaccctgg atctggaaga
     7801 ggccgagggt gctgtgggct accagcctga gtattccaag ttctgccgca tgagctacca
     7861 ggatccagct cagctctcat gcctgcccta cctggagcac gtgggccaca accacacata
     7921 caacatggca cccagtgccc tggactcagc cgacctgcca ccacccagtg ccctcaagaa
     7981 aggcagcaag gagaagcagg ctgacttcct ggacaagcag atgagccggg atgagcaccg
     8041 agcccgagcc atgaagatcc ctttcaccaa tgacaaaatc atcaacctgc ctgtggagga
     8101 gttcaatgaa ctgctgtcca aataccagtt gagtgaagcc cagctgagcc tcatccgaga
     8161 catccggcgc cggggcaaga acaagatggc ggcgcagaac tgccgcaagc gcaagctgga
     8221 caccatcctg aatctggagc gtgatgtgga ggacctgcag cgtgacaaag cccggctgct
     8281 gcgggagaaa gtggagttcc tgcgctccct gcgacagatg aagcagaagg tccagagcct
     8341 gtaccaggag gtgtttgggc ggctgcgaga tgagaacgga cgaccctact cgcccagtca
     8401 gtatgcgctc cagtacgccg gggacggcag tgtcctcctc atcccccgca cgatggccga
     8461 ccagcaggcc cggcggcagg agaggaagcc aaaggaccgg agaaagtgac caactttctt
     8521 gtacaaagtt gtgatatcca gcacagtggc ggccgctcga caatcaacct ctggattaca
     8581 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     8641 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     8701 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     8761 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     8821 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     8881 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     8941 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     9001 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     9061 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     9121 gtcggatctc cctttgggcc gcctccccgc ctggaattct accgggtagg ggaggcgctt
     9181 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     9241 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     9301 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     9361 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     9421 cagatggaca gcaccgctga gcaatggagg cgggtaggcc tttggggcag cggccaatag
     9481 cagttttgct ccttcgcttt cggggctcag gggcggggag ggggtgggtc ggggggcggg
     9541 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     9601 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     9661 tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     9721 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     9781 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     9841 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     9901 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     9961 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
    10021 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
    10081 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
    10141 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
    10201 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
    10261 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
    10321 gcgcacgacc ccatgcatct cgagggcccg gtacctttaa gaccaatgac ttacaaggca
    10381 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc
    10441 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
    10501 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    10561 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    10621 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
    10681 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
    10741 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
    10801 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
    10861 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
    10921 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
    10981 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
    11041 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
    11101 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
    11161 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
    11221 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
    11281 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
    11341 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
    11401 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
    11461 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
    11521 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
    11581 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
    11641 aaaatttaac gcgaatttta acaaaatatt aacgcttaca attt
//
