LOCUS       sequence-417834-        3971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  empty plasmid vector, non-fluorescent.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Hinnu M, Putrins M, Kogermann K, Kaldalu N, Tenson T
  TITLE     Fluorescent reporters give new insights into antibiotics-induced 
            nonsense and frameshift mistranslation.
  JOURNAL   Sci Rep. 2024 Mar 22;14(1):6883. doi: 10.1038/s41598-024-57597-8.
  PUBMED    38519558
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2024-03-22"; volume: "14(1):6883. doi"; pages: " 
            10.1038/s41598-024-57597-8"
COMMENT     Sequence Label: pSC101-AmpR EV
FEATURES             Location/Qualifiers
     source          1..3971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     120..139
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(join(198..989,990..1058))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     821..840
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1059..1163)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(1215..1242)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        join(1450..1455,1456..1472,1473..1478)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     terminator      1533..1627
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2119..2341
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2389..3339
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     terminator      complement(3877..3963)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agagcttgca tgcccaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
       61 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
      121 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
      181 atgcgtccgg cgtagagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
      241 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
      301 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
      361 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
      421 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
      481 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
      541 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
      601 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
      661 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
      721 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
      781 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
      841 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
      901 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
      961 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     1021 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     1081 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     1141 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     1201 accattatta tcataaaagg ccatccgtca ggatggcctt ctatgaatgc cttggctgcg
     1261 attcattctt tatatgaata aaattgttgt cagtttcacg tcttgtcctg ccatatcgcg
     1321 aaatttctgc gcaaaagcac aaaaaatttt tgcatctccc ccttgatgac gtgggttacg
     1381 accccattta gtagtcaacc gcagtgagtg agtctgacga ggccctttcg tcttcaagaa
     1441 ttctcatgtt tgacagctta tcatcgataa gctttaatgc gccatggatc gatagctggt
     1501 cgacaggact ctggggttcg agagctcgct tggactcctg ttgatagatc cagtaatgac
     1561 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg
     1621 tgagaatcca agcactaggg acagtaagac gggtaagcct gttgatgata ccgctgcctt
     1681 actgggtgca ttagccagtc tgaatgacct gtcacgggat aatccgaagt ggtcagactg
     1741 gaaaatcaga gggcaggaac tgctgaacag caaaaagtca gatagcacca catagcagac
     1801 ccgccataaa acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc
     1861 ttgcatgaat ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg
     1921 tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag
     1981 acaaaaggaa tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg
     2041 ttttaaggtc tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc
     2101 ggaactgact aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt
     2161 tattctttct ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa
     2221 ggtctagcgg aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag
     2281 cagaatttac agatacccac aactcaaagg aaaaggacta gtaattatca ttgactagcc
     2341 catctcaatt ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta
     2401 gttgttttca aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc
     2461 aagctaattt tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa
     2521 gaacggacgg tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa
     2581 aatgcttatg gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc
     2641 aggaatcctt tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca
     2701 agcgaaaaat tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa
     2761 aaattcataa aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg
     2821 atttatgagt ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag
     2881 attagccttg atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa
     2941 aggcttaacc aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg
     3001 aaattggtgg ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta
     3061 gatagacaaa tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac
     3121 aaaataccaa caaccattac atcagattcc tacctacata acggactaag aaaaacacta
     3181 cacgatgctt taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac
     3241 atgcaaagta agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc
     3301 acactagaga acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc
     3361 tatcagtgaa gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc
     3421 aaagggaaaa ctgtccatat gcacagatga aaacggtgta aaaaagatag atacatcaga
     3481 gcttttacga gtttttggtg cattcaaagc tgttcaccat gaacagatcg acaatgtaac
     3541 agatgaacag catgtaacac ctaatagaac aggtgaaacc agtaaaacaa agcaactaga
     3601 acatgaaatt gaacacctga gacaacttgt tacagctcaa cagtcacaca tagacagcct
     3661 gaaacaggcg atgctgctta tcgaatcaaa gctgccgaca acacgggagc cagtgacgcc
     3721 tcccgtgggg aaaaaatcat ggcaattctg gaagaaatag cgctttcagc cggcaaaccg
     3781 gctgaagccg gatctgcgat tctgataaca aactagcaac accagaacag cccgtttgcg
     3841 ggcagcaaaa cccgtaccct aggtctaggg cggcggattt gtcctactca ggagagcgtt
     3901 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     3961 ttgatgcctc t
//
