LOCUS       sequence-417838-        9939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLAG-tagged NRF2-E79Q driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   LaPak KM, Saeidi S, Bok I, Wamsley NT, Plutzer IB, Bhatt DP, Luo J, 
            Ashrafi G, Ben Major M
  TITLE     Proximity proteomic analysis of the NRF family reveals the 
            Parkinson's disease protein ZNF746/PARIS as a co-complexed repressor
            of NRF2.
  JOURNAL   Sci Signal. 2023 Dec 12;16(815):eadi9018. doi: 
            10.1126/scisignal.adi9018. Epub 2023 Dec 12.
  PUBMED    38085818
REFERENCE   2  (bases 1 to 9939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2023-12-12"; volume: "16(815):eadi9018. doi"; 
            pages: " 10.1126/scisignal.adi9018. Epub 2023 Dec 12"
COMMENT     Sequence Label: pHAGE-NRF2-E79Q
FEATURES             Location/Qualifiers
     source          1..9939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             5..638
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    685..810
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1307..1540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1725..1769
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2830..2839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2839..2862
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2872..2896
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4712..4736)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          4810..5039
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5022..5041
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(5112..5686,5687..5689,5690..5698)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5279..5296)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5506..5525
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5718..6317
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5718..5737)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6214..6234
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6332..6920
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6385..6405)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6803..6814)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6995..7228
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7347..7365)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7433..7537
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7538..7606,7607..8398)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7756..7775)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8569..9157
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9058..9077
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9311..9328
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9569..9590
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9605..9610,9611..9628,9629..9635)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9643..9659
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9648..9670
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9667..9683
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9667..9683
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9733..9752
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
       61 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      121 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      181 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      241 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      301 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      361 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
      421 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
      481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      661 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      721 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      781 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      841 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      901 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      961 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1021 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1081 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1141 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1201 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1261 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1321 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1381 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1441 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1501 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1561 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1621 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1681 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1741 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1801 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1861 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1921 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1981 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccataccggt
     2821 cagctcgagg ccaccatgga ctacaaggac gacgacgaca agggcggatc aacaagtttg
     2881 tacaaaaaag ttggcatgat ggacttggag ctgccgccgc cgggactccc gtcccagcag
     2941 gacatggatt tgattgacat actttggagg caagatatag atcttggagt aagtcgagaa
     3001 gtatttgact tcagtcagcg acggaaagag tatgagctgg aaaaacagaa aaaacttgaa
     3061 aaggaaagac aagaacaact ccaaaaggag caagagaaag cctttttcgc tcagttacaa
     3121 ctagatgaac agacaggtga atttctccca attcagccag cccagcacat ccagtcagaa
     3181 accagtggat ctgccaacta ctcccaggtt gcccacattc ccaaatcaga tgctttgtac
     3241 tttgatgact gcatgcagct tttggcgcag acattcccgt ttgtagatga caatgaggtt
     3301 tcttcggcta cgtttcagtc acttgttcct gatattcccg gtcacatcga gagcccagtc
     3361 ttcattgcta ctaatcaggc tcagtcacct gaaacttctg ttgctcaggt agcccctgtt
     3421 gatttagacg gtatgcaaca ggacattgag caagtttggg aggagctatt atccattcct
     3481 gagttacagt gtcttaatat tgaaaatgac aagctggttg agactaccat ggttccaagt
     3541 ccagaagcca aactgacaga agttgacaat tatcattttt actcatctat accctcaatg
     3601 gaaaaagaag taggtaactg tagtccacat tttcttaatg cttttgagga ttccttcagc
     3661 agcatcctct ccacagaaga ccccaaccag ttgacagtga actcattaaa ttcagatgcc
     3721 acagtcaaca cagattttgg tgatgaattt tattctgctt tcatagctga gcccagtatc
     3781 agcaacagca tgccctcacc tgctacttta agccattcac tctctgaact tctaaatggg
     3841 cccattgatg tttctgatct atcactttgc aaagctttca accaaaacca ccctgaaagc
     3901 acagcagaat tcaatgattc tgactccggc atttcactaa acacaagtcc cagtgtggca
     3961 tcaccagaac actcagtgga atcttccagc tatggagaca cactacttgg cctcagtgat
     4021 tctgaagtgg aagagctaga tagtgcccct ggaagtgtca aacagaatgg tcctaaaaca
     4081 ccagtacatt cttctgggga tatggtacaa cccttgtcac catctcaggg gcagagcact
     4141 cacgtgcatg atgcccaatg tgagaacaca ccagagaaag aattgcctgt aagtcctggt
     4201 catcggaaaa ccccattcac aaaagacaaa cattcaagcc gcttggaggc tcatctcaca
     4261 agagatgaac ttagggcaaa agctctccat atcccattcc ctgtagaaaa aatcattaac
     4321 ctccctgttg ttgacttcaa cgaaatgatg tccaaagagc agttcaatga agctcaactt
     4381 gcattaattc gggatatacg taggaggggt aagaataaag tggctgctca gaattgcaga
     4441 aaaagaaaac tggaaaatat agtagaacta gagcaagatt tagatcattt gaaagatgaa
     4501 aaagaaaaat tgctcaaaga aaaaggagaa aatgacaaaa gccttcacct actgaaaaaa
     4561 caactcagca ccttatatct cgaagttttc agcatgctac gtgatgaaga tggaaaacct
     4621 tattctccta gtgaatactc cctgcagcaa acaagagatg gcaatgtttt ccttgttccc
     4681 aaaagtaaga agccagatgt taagaaatag tacccaactt tcttgtacaa agtggttgat
     4741 taagatccac tagtaacggc cgccagtgtg ctggaattaa ttcgctgtct gcgagggcca
     4801 gctgttgggg tgagtactcc ctctcaaaag cgggcatgac ttctgcgcta agattgtcag
     4861 tttccaaaaa cgaggaggat ttgatattca cctggcccgc ggtgatgcct ttgagggtgg
     4921 ccgcgtccat ctggtcagaa aagacaatct ttttgttgtc aagcttgagg tgtggcaggc
     4981 ttgagatctg gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg
     5041 tgtccactcc caggtccaac tgcaggtcga gcatgcatct agggcggcca attccgcccc
     5101 tctccccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     5161 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     5221 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     5281 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     5341 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     5401 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     5461 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     5521 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     5581 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     5641 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaaccca
     5701 caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     5761 acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     5821 acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     5881 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     5941 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     6001 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     6061 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     6121 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     6181 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     6241 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     6301 gcaagcccgg tgcctgatat cgatagatcc taatcaacct ctggattaca aaatttgtga
     6361 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6421 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6481 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6541 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6601 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6661 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6721 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6781 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6841 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6901 cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta
     6961 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7021 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7081 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7141 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7201 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7261 ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg
     7321 gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     7381 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     7441 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7501 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7561 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7621 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7681 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     7741 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     7801 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     7861 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     7921 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     7981 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8041 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8101 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8161 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8221 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8281 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8341 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8401 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8461 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8521 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8581 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8641 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8701 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8761 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8821 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8881 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8941 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9001 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9061 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9121 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9181 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9241 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9301 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9361 tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt
     9421 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     9481 cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact
     9541 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     9601 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     9661 ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac
     9721 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
     9781 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
     9841 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
     9901 aaagaaattg tatttgttaa atatgtacta caaacttag
//
