LOCUS       sequence-417839-        9966 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLAG-tagged PGUS driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9966)
  AUTHORS   LaPak KM, Saeidi S, Bok I, Wamsley NT, Plutzer IB, Bhatt DP, Luo J, 
            Ashrafi G, Ben Major M
  TITLE     Proximity proteomic analysis of the NRF family reveals the 
            Parkinson's disease protein ZNF746/PARIS as a co-complexed repressor
            of NRF2.
  JOURNAL   Sci Signal. 2023 Dec 12;16(815):eadi9018. doi: 
            10.1126/scisignal.adi9018. Epub 2023 Dec 12.
  PUBMED    38085818
REFERENCE   2  (bases 1 to 9966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2023-12-12"; volume: "16(815):eadi9018. doi"; 
            pages: " 10.1126/scisignal.adi9018. Epub 2023 Dec 12"
COMMENT     Sequence Label: pHAGE-PGUS
FEATURES             Location/Qualifiers
     source          1..9966
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             5..638
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    685..810
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1307..1540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1725..1769
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2830..2839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2839..2862
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2872..2896
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2926..4737
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     protein_bind    complement(4739..4763)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          4837..5066
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5049..5068
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(5139..5713,5714..5716,5717..5725)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5306..5323)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5533..5552
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5745..6344
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5745..5764)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6241..6261
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6359..6947
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6412..6432)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6830..6841)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7022..7255
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7374..7392)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7460..7564
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7565..7633,7634..8425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7783..7802)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8596..9184
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9085..9104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9338..9355
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9596..9617
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9632..9637,9638..9655,9656..9662)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9670..9686
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9675..9697
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9694..9710
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9694..9710
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9760..9779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
       61 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      121 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      181 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      241 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      301 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      361 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
      421 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
      481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      661 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      721 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      781 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      841 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      901 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      961 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1021 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1081 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1141 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1201 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1261 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1321 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1381 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1441 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1501 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1561 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1621 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1681 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1741 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1801 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1861 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1921 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1981 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccataccggt
     2821 cagctcgagg ccaccatgga ctacaaggac gacgacgaca agggcggatc aacaagtttg
     2881 tacaaaaaag caggctcatt taactttaag aaggagatat ataccatggt ccgtcctgta
     2941 gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc
     3001 gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt
     3061 gctgtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc
     3121 aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg
     3181 ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg
     3241 gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg
     3301 aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg
     3361 ggaatggtga ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt
     3421 aactatgccg gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac
     3481 gatatcaccg tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag
     3541 gtggtggcca atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca
     3601 actggacaag gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt
     3661 gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac
     3721 ccgcttcgcg tcggcatccg gtcagtggca gtgaagggcg aacagttcct gattaaccac
     3781 aaaccgttct actttactgg ctttggtcgt catgaagatg cggacttgcg tggcaaagga
     3841 ttcgataacg tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc
     3901 taccgtacct cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc
     3961 atcgtggtga ttgatgaaac tgctgctgtc ggctttaacc tctctttagg cattggtttc
     4021 gaagcgggca acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag
     4081 caagcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg
     4141 gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc
     4201 gcgccactgg cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat
     4261 gtaatgttct gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg
     4321 aaccgttatt acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg
     4381 gaaaaagaac ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac
     4441 ggcgtggata cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat
     4501 cagtgtgcat ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt
     4561 gaacaggtat ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt
     4621 aacaagaaag ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa
     4681 aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaataatac
     4741 ccagctttct tgtacaaagt ggttgattaa gatccactag taacggccgc cagtgtgctg
     4801 gaattaattc gctgtctgcg agggccagct gttggggtga gtactccctc tcaaaagcgg
     4861 gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct
     4921 ggcccgcggt gatgcctttg agggtggccg cgtccatctg gtcagaaaag acaatctttt
     4981 tgttgtcaag cttgaggtgt ggcaggcttg agatctggcc atacacttga gtgacaatga
     5041 catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc aggtcgagca
     5101 tgcatctagg gcggccaatt ccgcccctct cccccccccc cctctccctc ccccccccct
     5161 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     5221 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     5281 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     5341 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     5401 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     5461 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     5521 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     5581 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     5641 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     5701 acacgatgat aagcttgcca caacccacaa ggagacgacc ttccatgacc gagtacaagc
     5761 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
     5821 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
     5881 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     5941 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     6001 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     6061 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     6121 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     6181 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     6241 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     6301 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgatatcga tagatcctaa
     6361 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6421 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6481 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6541 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6601 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6661 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6721 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6781 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6841 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6901 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
     6961 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     7021 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     7081 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7141 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7201 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7261 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7321 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     7381 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     7441 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7501 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7561 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7621 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7681 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7741 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7801 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7861 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7921 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7981 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8041 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8101 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8161 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8221 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8281 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8341 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8401 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8461 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8521 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8581 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8641 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8701 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8761 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8821 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8881 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8941 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9001 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9061 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9121 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9181 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9241 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9301 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9361 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9421 caagctcatg gctgactaat ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9481 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9541 ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     9601 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     9661 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
     9721 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9781 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     9841 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     9901 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     9961 acttag
//
